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WO2011099279A1 - Procédé de gestion de la précision de distribution, procédé de correction d'un volume distribué, procédé de gestion de performances d'agitation, dispositif d'analyse automatique et kit d'analyse - Google Patents

Procédé de gestion de la précision de distribution, procédé de correction d'un volume distribué, procédé de gestion de performances d'agitation, dispositif d'analyse automatique et kit d'analyse Download PDF

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Publication number
WO2011099279A1
WO2011099279A1 PCT/JP2011/000721 JP2011000721W WO2011099279A1 WO 2011099279 A1 WO2011099279 A1 WO 2011099279A1 JP 2011000721 W JP2011000721 W JP 2011000721W WO 2011099279 A1 WO2011099279 A1 WO 2011099279A1
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WO
WIPO (PCT)
Prior art keywords
dispensing
reagent
raman
amount
unit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2011/000721
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English (en)
Japanese (ja)
Inventor
貴行 水谷
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Beckman Coulter Inc
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Beckman Coulter Inc
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Publication date
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Publication of WO2011099279A1 publication Critical patent/WO2011099279A1/fr
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00594—Quality control, including calibration or testing of components of the analyser
    • G01N35/00613—Quality control
    • G01N35/00623—Quality control of instruments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/026—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having blocks or racks of reaction cells or cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016—Control of the volume dispensed or introduced

Definitions

  • the present invention relates to a dispensing accuracy management method, a dispensing amount correction method, a stirring performance management method, an automatic analyzer, and an analysis kit.
  • automatic analyzers analyze standard samples and quality control samples at the start of one day of analysis, or when reagents are changed to reagent bottles with different lot numbers due to running out of reagents, and the operator performs measurements based on the analysis results After confirming the accuracy from the values, analysis of the specimen is continued.
  • the automatic analyzer performs accuracy confirmation, that is, accuracy management by automatically analyzing the quality control sample every time a predetermined number of samples are analyzed.
  • An automatic analyzer that automatically determines whether or not reagent replacement or calibration curve calibration is necessary, or whether or not a quality control sample needs to be readjusted based on the measurement results when the measurement result of a quality control sample falls outside the control range Is disclosed (for example, see Patent Document 1).
  • JP 2009-36515 A Japanese Patent No. 4006203
  • the same operation is performed with a plurality of different dispensing amounts, and after confirming the linearity, the dispensing amount is corrected. It takes time and effort.
  • the present invention has been made in view of the above, and a dispensing accuracy management method, a dispensing amount correction method, a stirring performance management method, which can greatly reduce the maintenance time including confirmation of dispensing accuracy,
  • An object is to provide an automatic analyzer and an analysis kit.
  • the dispensing accuracy management method of the present invention is an automatic method for analyzing a reaction product of a sample and a reagent dispensed into a reaction container by a dispensing mechanism with Raman scattered light.
  • a dispensing accuracy management method in an analyzer comprising: a dispensing step in which different Raman reagents are dispensed by a dispensing probe in one reaction container; and an agitating step in which the Raman reagent in the dispensed reaction container is agitated And for each Raman reagent in the reaction vessel, a measurement step for photometric analysis using a Raman spectrometer, an analysis step for determining the concentration of each Raman reagent based on the data measured in the measurement step, and the analysis step Based on the calculation step for calculating the dispensing amount of each Raman reagent from the concentration of each Raman reagent, and whether the dispensing amount is abnormal based on the dispensing amount calculated in the calculation step
  • the dispensing step dispenses using two or more reagent probes as the dispensing probes
  • the calculating step includes the concentration of each Raman reagent.
  • the dispensing amount of each reagent probe is calculated from the determination step, the determination step determines whether the dispensing amount of each reagent probe calculated by the calculation step is within a predetermined range, and the output step includes the determination When it is determined in the step that the dispensing amount of each reagent probe is outside the predetermined range, a dispensing abnormality alert is output for the reagent probe outside the predetermined range.
  • the dispensing step and the measuring step are repeated a predetermined number of times, and the calculating step is performed based on the concentration of each Raman reagent obtained in the analyzing step.
  • An average dispensing amount of each reagent probe is calculated, and the determining step determines whether the average dispensing amount of each reagent probe is within a predetermined range.
  • the dispensing step and the measuring step are repeated a predetermined number of times, and the calculating step is based on the concentration of each Raman reagent determined in the analyzing step. And calculating a variation coefficient of the dispensing amount of each reagent probe based on the dispensing amount, and the determination step determines whether or not the variation coefficient is a predetermined value or less, In the output step, when it is determined in the determination step that the coefficient of variation is larger than a predetermined value, a dispensing abnormality alert is output for each dispensing probe.
  • the dispensing step involves dispensing the same amount of different Raman reagents into one reaction vessel with one dispensing probe, and the calculating step. Calculates the dispensing amount of each dispensing probe from the concentration of each Raman reagent, calculates a variation coefficient based on the dispensing amount, and the determination step determines whether the variation coefficient is a predetermined value or less. The output step outputs an alert indicating that the dispensing amount is abnormal when it is determined by the determination step that the coefficient of variation is greater than a predetermined value.
  • the calculating step calculates an average dispensing amount of the dispensing probe from the concentration of each Raman reagent obtained in the analyzing step, and the determining step Determines whether the average dispensing amount is within a predetermined range, and the output step is dispensing when the determination step determines that the average dispensing amount of the dispensing probe is outside the predetermined range. It is characterized by outputting a volume abnormality alert.
  • the dispensing step is performed by dispensing different Raman reagents in one reaction vessel with different dispensing probes by changing the dispensing amount.
  • the calculating step calculates a dispensed amount of each Raman reagent from the concentration of each Raman reagent obtained in the analyzing step, and the determining step includes each dispensed amount calculated in the calculating step and a scheduled amount. It is determined whether or not the relationship with the dispensing amount maintains linearity, and the output step outputs an alert of dispensing amount abnormality when it is determined by the determining step that there is a deviation from linearity.
  • the dispensing step and the measuring step are repeated a predetermined number of times, and the calculating step is performed based on the concentration of each Raman reagent determined in the analyzing step.
  • the average dispensing amount of the Raman reagent is calculated respectively, and the determination step determines whether or not the relationship between each average dispensing amount calculated by the calculation step and the planned dispensing amount maintains linearity.
  • the dispensing accuracy management method of the present invention is characterized in that, in the above-mentioned invention, the Raman reagent has a Raman scattering spectrum different from other Raman reagents used simultaneously in the measurement step.
  • the Raman reagent may be gold, silver, copper or platinum particles modified with a Raman active molecule, or silver / gold, platinum / gold, copper / gold alloy particles. It is characterized by being.
  • the dispensing amount correction method in the automatic analyzer of the present invention uses an analysis kit in which a predetermined amount of all the Raman reagents used in the dispensing accuracy management method of the automatic analyzer described above are accommodated in individual reaction containers.
  • a dispensing amount correction method in an automatic analyzer comprising: an analysis kit measuring step in which a reaction container containing each Raman reagent is transported to a photometric means, and the concentration of each Raman reagent is directly measured by a Raman spectrometer; Based on the concentration of each Raman reagent measured in the analysis kit measurement step, the dispensing accuracy management step for managing the dispensing accuracy of the automatic analyzer and the dispensing mechanism in which the dispensing accuracy is abnormal in the dispensing management step. And a correction step for performing correction.
  • the stirring performance management method of the automatic analyzer of the present invention is a stirring performance management method in an automatic analyzer that analyzes a reaction product of a sample and a reagent dispensed in a reaction container by a dispensing mechanism with Raman scattered light.
  • a magnetic particle dispensing step for dispensing a predetermined amount of magnetic particles, a stirring step for stirring the solution in the reaction vessel in a predetermined manner, and the magnetic particles in the reaction vessel by a magnetic material installed outside the reaction vessel Collecting each Raman reagent based on the data collected in the measurement step, the measurement step of performing photometric analysis of each Raman reagent coupled to the collected magnetic particles with a Raman spectrometer, and the data measured in the measurement step.
  • An analysis step for obtaining a reagent concentration a calculation step for calculating a reaction ratio with the magnetic particles from the concentration of each Raman reagent obtained in the analysis step, and determining whether the reaction ratio is within a predetermined range
  • the automatic analyzer of the present invention is an automatic analyzer for analyzing a reaction product of a sample and a reagent dispensed into a reaction container by a dispensing mechanism by Raman spectroscopy, and one reaction container at a predetermined timing.
  • Control means for dispensing different Raman reagents with a dispensing probe and stirring them, and then controlling each Raman reagent in the reaction vessel by photometric analysis with a Raman spectrometer and analyzing the concentration of each Raman reagent
  • Control means for controlling photometric analysis with a Raman spectrometer for each Raman reagent in the reaction vessel, analysis means for determining the concentration of the Raman reagent based on the data measured by the photometric analysis, and the analysis means
  • a calculating means for calculating a dispensed amount of the Raman reagent from the concentration of the Raman reagent determined by the step, and based on the dispensed amount of the Raman reagent calculated by the calculating means.
  • Min judging means for judging whether or not a Note abnormal, when said determination means determines that the abnormal dispensing, characterized by comprising output means for outputting a dispensing abnormality of the alert, the.
  • the control unit dispenses different Raman reagents into two reaction probes in one reaction container, and the dispensed reaction container contains After stirring the Raman reagent, control is performed so that each Raman reagent is photometrically analyzed by a Raman spectrometer, and the calculation means calculates the dispensing amount of each reagent probe from the concentration of each Raman reagent obtained by the analysis means.
  • the determination unit determines whether the dispensing amount of each reagent probe calculated by the calculation unit is within a predetermined range, and the output unit determines whether the dispensing unit has a predetermined dispensing amount of each reagent probe. When it is determined that it is out of the range, an alert indicating that the dispensing amount is abnormal is output for the reagent probe outside the predetermined range.
  • control unit dispenses the same amount of different Raman reagents into one reaction container with one dispensing probe, and dispenses the reaction. After the Raman reagent in the container is stirred, control is performed so that each Raman reagent is photometrically analyzed by a Raman spectrometer, and the calculation means determines each of the dispensing probes from the concentration of each Raman reagent obtained by the analysis means.
  • An amount of injection is obtained, a coefficient of variation is calculated based on the dispensed amount, the determination unit determines whether or not the variation coefficient is equal to or greater than a predetermined value, and the output unit includes When it is determined that the value is greater than or equal to a predetermined value, a dispensing amount abnormality alert is output.
  • the control unit dispenses a different Raman reagent with one dispensing probe into one reaction container by changing a dispensing amount.
  • the control is performed so that each Raman reagent is subjected to photometric analysis with a Raman spectrometer, and the calculation means calculates each Raman reagent from the concentration of each Raman reagent obtained by the analysis means.
  • the determination means determines whether or not the relationship between each dispensing amount calculated by the calculating means and the planned dispensing amount maintains linearity
  • the output means includes: When the determination unit determines that each dispensed amount is deviated from linearity, an alert of a dispensed amount abnormality is output.
  • the automatic analyzer according to the present invention is the automatic analyzer according to the above invention, wherein the control means photometrically measures each Raman reagent with a Raman spectrometer for each reaction container constituting a predetermined amount of the Raman reagent. Control to analyze, the analysis means obtains the concentration of each Raman reagent based on the data obtained by measurement of the analysis kit, and when the determination means determines that the dispensing probe is abnormal dispensing, Correction means for correcting the calibration curve of each Raman reagent stored in the storage means based on the concentration of each Raman reagent obtained by analysis of the analysis kit.
  • the analysis kit of the present invention is an analysis kit for correcting the dispensing amount in the above-described automatic analyzer, and the analysis kit includes all Raman reagents used for dispensing accuracy management in the automatic analyzer. A predetermined amount is stored in an individual reaction vessel.
  • the automatic analyzer of the present invention is an automatic analyzer that analyzes a reaction product of a sample and a reagent dispensed into a reaction container by a dispensing mechanism with Raman scattered light, and performs one reaction at a predetermined timing.
  • Dispensing two or more types of Raman reagent modified with target particles into a container, and further dispensing a magnetic particle modified with a reactive substance that binds to the target molecule, and the reaction in a predetermined manner A stirring means for stirring the Raman reagent and magnetic particles dispensed in the container in a predetermined manner; a magnetic collecting means for collecting magnetic particles in the reaction container; and a magnetic particle collected by the magnetic collecting means; Photometric means for photometric analysis of each Raman reagent to be bound by a Raman spectrometer, analysis means for analyzing the concentration of each Raman reagent based on data photometrically measured by the photometric means, and each Raman test analyzed by the analytical means A calculation means for calculating a reaction ratio with the magnetic particles from the concentration of the liquid, a determination means for determining whether the reaction ratio is within a predetermined range, and the determination means determines that the reaction ratio is out of the predetermined range. Output means for outputting an alert of abnormal stirring.
  • the present invention produces an effect that a dispensing reagent in a dispensing part can be easily determined by dispensing a Raman reagent by a dispensing part and analyzing the dispensed Raman reagent.
  • FIG. 1 is a schematic diagram showing the configuration of the automatic analyzer according to the first embodiment of the present invention.
  • FIG. 2 is a schematic diagram showing the configuration of the first reagent dispensing unit.
  • FIG. 3 is a diagram for explaining the reaction between the measurement object in the specimen and the magnetic particles and label particles contained in the reagent.
  • FIG. 4 is a diagram showing a configuration of the magnetic flux collecting mechanism shown in FIG.
  • FIG. 5 is a diagram showing the configuration of the photometry unit shown in FIG.
  • FIG. 6 is a diagram showing the Raman shift of each Raman reagent.
  • FIG. 7 is a schematic diagram illustrating a method for generating Raman standard particles according to the first embodiment.
  • FIG. 1 is a schematic diagram showing the configuration of the automatic analyzer according to the first embodiment of the present invention.
  • FIG. 2 is a schematic diagram showing the configuration of the first reagent dispensing unit.
  • FIG. 3 is a diagram for explaining the reaction between the measurement object in the specimen and the magnetic particles
  • FIG. 8 is a dispensing operation diagram of a Raman reagent by each reagent probe according to the first embodiment.
  • FIG. 9 is a flowchart of the dispensing accuracy management process according to the first embodiment.
  • FIG. 10 is a schematic diagram showing the configuration of the automatic analyzer according to the second embodiment of the present invention.
  • FIG. 11 is a diagram illustrating the dispensing operation of the Raman reagent by the dispensing probe according to the second embodiment.
  • FIG. 12 is a flowchart of the dispensing accuracy management process according to the second embodiment.
  • FIG. 13 is a schematic diagram showing the configuration of the automatic analyzer according to the third embodiment of the present invention.
  • FIG. 14 is a flowchart of the dispensing accuracy management process according to the third embodiment.
  • FIG. 15 is a schematic diagram illustrating a configuration of an automatic analyzer according to the fourth embodiment of the present invention.
  • FIG. 16 is a flowchart of the dispensing accuracy management process according to the fourth embodiment.
  • FIG. 17 is a schematic diagram showing the configuration of the automatic analyzer according to the fifth embodiment of the present invention.
  • FIG. 18 is a view showing an analysis kit used in the automatic analyzer of FIG.
  • FIG. 19 is a flowchart of the dispensing unit correction process according to the fifth embodiment.
  • FIG. 20 is a flowchart of the analysis kit analysis process of FIG.
  • FIG. 21 is a schematic diagram showing the configuration of the automatic analyzer according to the sixth embodiment of the present invention.
  • FIG. 22 is an operation diagram of the stirring performance management process according to the sixth embodiment.
  • FIG. 23 is a flowchart of the stirring performance management process according to the sixth embodiment.
  • FIG. 1 is a schematic diagram showing a configuration of an automatic analyzer 1 according to the first embodiment of the present invention.
  • the automatic analyzer 1 according to the first embodiment of the present invention emits a laser beam to an aggregate of a complex of a specimen and a labeling substance, and the surface enhanced Raman from the aggregate.
  • a measurement mechanism 2 that measures scattered light and a control mechanism 4 that controls the entire automatic analyzer 1 including the measurement mechanism 2 and analyzes the measurement results in the measurement mechanism 2 are provided.
  • the automatic analyzer 1 automatically performs analysis on a plurality of samples by cooperation of these two mechanisms.
  • the measurement mechanism 2 will be described.
  • the measurement mechanism 2 is roughly divided into a sample transfer lane 21, a sample dispensing unit 22, a chip storage unit 23, a reaction container transfer lane 24, a first reagent storage 25, a second reagent storage 26, a first reagent dispensing unit 27, and 28, second reagent dispensing sections 29 and 30, a magnetic collection table 31, a first container transfer section 32, a second container transfer section 33, and a photometry section 34.
  • the sample transfer lane 21 includes a plurality of sample racks 21b that hold a plurality of sample containers 21a containing samples and sequentially transfer them in the direction of the arrows in the figure.
  • the specimen stored in the specimen container 21a is a body fluid such as blood, urine, saliva collected from the specimen provider.
  • the specimen dispensing unit 22 includes an arm that freely moves up and down in the vertical direction and rotates around the vertical line passing through the base end of the sample dispensing unit 22 as a central axis. A tip for aspirating and discharging the specimen is detachably attached to the tip of the arm.
  • the specimen dispensing unit 22 includes an intake / exhaust mechanism using an unillustrated intake / exhaust syringe or piezoelectric element. The sample dispensing unit 22 sucks the sample from the sample container 21a transferred to the predetermined position on the sample transfer lane 21 by the sample probe, rotates the arm clockwise in the drawing, and the reaction container transfer lane 24. The specimen is discharged into the upper reaction container 20.
  • the reaction container 20 is a synthetic resin container that is disposable for each analysis.
  • the chip storage unit 23 is provided with a chip case in which a plurality of chips are aligned.
  • the chip storage unit 23 supplies the chip from this case to the nozzle of the sample dispensing unit 22.
  • This tip is a disposable sample dispensing tip that is attached to the tip of the nozzle of the sample dispensing unit 22 and is exchanged for each sample dispensing in order to prevent carryover when measuring an infectious disease item.
  • the reaction container transfer lane 24 holds a plurality of reaction containers 20, and each reaction container 20 is placed at the sample dispensing position, the first reagent dispensing position, and the second reagent dispensing position along the direction of the arrow in the figure. Transport sequentially.
  • the first reagent storage 25 a plurality of first reagent containers 25a containing the first reagent to be dispensed in the reaction container 20 on the reaction container transfer lane 24 are stored, and around the rotation axis passing through the center by a drive unit (not shown). To be rotated. By this rotation, the first reagent storage 25 conveys the stored first reagent container 25a in the circumferential direction indicated by the arrow.
  • the measurement object in the specimen includes, for example, antibodies, proteins, peptides, amino acids, carbohydrates, hormones, steroids, vitamins, bacteria, DNA, RNA, cells, viruses, and any antigenic substances, haptens, antibodies, and combinations thereof and so on.
  • the first reagent is a reagent containing a magnetic substance solid-phased on a reaction substance that binds to a measurement target in a sample to be analyzed or a measurement target in the sample to be analyzed or an analog thereof.
  • the first reagent storage 25 accommodates a Raman reagent container 25b that accommodates a Raman reagent that is a dispensing accuracy confirmation reagent of first reagent dispensing units 27 and 28 described later.
  • the second reagent storage 26 stores a plurality of second reagent containers 26 a that store the second reagent to be dispensed into the reaction container 20 on the reaction container transfer lane 24.
  • the second reagent storage 26 is rotated around a rotation axis passing through the center by a drive unit (not shown), and conveys the stored second reagent container 26a in the circumferential direction indicated by an arrow.
  • the second reagent is a reagent containing a labeling substance that binds to the measurement target in the specimen, or a labeling substance that binds the measurement target in the specimen that is the analysis target or an analog thereof.
  • the labeling substance is a particle containing gold or silver having an atomic level surface roughness, and the surface of the particle containing gold or silver is a reactive substance that can bind to the measurement object or an analysis object. An object to be measured in the specimen or an analog thereof is coated.
  • the first reagent and the second reagent may be opposite reagents. That is, magnetic particles in which a first reagent is a reagent containing a labeling substance that binds to a measurement target in a sample, and a second reagent is a solid phase of a reactive substance that binds to the measurement target in the sample to be analyzed. It may be a reagent containing.
  • the first reagent storage 26 accommodates a reagent container 26b that contains a Raman reagent that is a reagent for confirming the dispensing accuracy of second reagent dispensing units 30 and 31, which will be described later.
  • the first reagent dispensing units 27 and 28 have a probe for aspirating and discharging the first reagent attached to the distal end portion, ascending and descending in the vertical direction, and rotating about a vertical line passing through the base end portion of the first reagent.
  • a freely performing arm is provided.
  • the first reagent dispensing units 27 and 28 suck the first reagent in the first reagent container 25a moved to a predetermined position by the first reagent storage 25 with the probe, rotate the arm, and the reaction container transfer lane 24. To dispense into the reaction container 20 conveyed to the first reagent discharge position.
  • the first reagent dispensing unit 27 includes a dispensing probe 27a that dispenses the first reagent, an arm 27b that supports the dispensing probe 27a, a connecting unit 27c that connects the arm and the drive unit, A drive part 27d for transferring the probe 27a, a pipe 27e for sending an extruding liquid L1 for transmitting pressure, a syringe pump 27f for applying suction and discharge pressure of the first reagent, and a plunger drive part 27h for driving the syringe pump 27f And an electromagnetic valve 27i that manages the liquid feeding operation of the extrusion liquid L1, a liquid feeding pump 27j that feeds the extrusion liquid L1, and a tank 27k that stores the extrusion liquid L1.
  • the first reagent dispensing unit 28 has the same configuration as that of the first reagent dispensing unit 27, and includes a dispensing probe 28a that dispenses the first reagent, an arm 28b that supports the dispensing probe 28a, an arm and a drive unit.
  • a plunger driving section 28h for driving the liquid, an electromagnetic valve 28i for managing the liquid feeding operation of the extrusion liquid L1, a liquid feeding pump 28j for feeding the extrusion liquid L1, and a tank 28k for storing the extrusion liquid L1.
  • the second reagent dispensing units 29 and 30 have the same configuration as the first reagent dispensing units 27 and 28, and the reagents in the second reagent container 26a moved to the predetermined position by the second reagent storage 26 The sample is sucked by the probe, the arm is swung, and dispensed to the reaction container 20 conveyed to the second reagent discharge position by the reaction container transfer lane 24.
  • the second reagent dispensing unit 29 has the same configuration as the first reagent dispensing unit 27, a dispensing probe 29a that dispenses the second reagent, an arm 29b that supports the dispensing probe 29a, an arm and a drive unit.
  • a plunger driving unit 29h for driving the liquid, an electromagnetic valve 29i for managing the liquid feeding operation of the extrusion liquid L1, a liquid feeding pump 29j for feeding the extrusion liquid L1, and a tank 29k for storing the extrusion liquid L1.
  • the second reagent dispensing unit 30 has the same configuration as the first reagent dispensing unit 27, and includes a dispensing probe 30a that dispenses the second reagent, an arm 30b that supports the dispensing probe 30a, an arm and a drive unit.
  • a plunger driving unit 30h for driving the liquid, an electromagnetic valve 30i for managing the liquid feeding operation of the extrusion liquid L1, a liquid feeding pump 30j for feeding the extrusion liquid L1, and a tank 30k for storing the extrusion liquid L1.
  • the first container transfer unit 32 transfers the reaction container 20 into which the sample, the first reagent, and the second reagent have been dispensed from the reaction container transfer lane 24 to the magnetism collection table 31 at a predetermined timing.
  • the first container transfer unit 28 includes an arm having a gripping device that can grip the reaction container 20 at the tip.
  • the first container transfer section 32 moves the reaction container 20 from the reaction container transfer lane 24 to the magnetic collecting table by moving the arm up and down in the vertical direction by a driving means such as a stepping motor and rotating the base end of the first container as a rotation axis. Transfer to 31.
  • the magnetic flux collecting table 31 can accommodate a plurality of reaction vessels 20 along the circumferential direction, and moves the reaction vessels 20 along the circumferential direction by rotating.
  • the magnetic flux collecting table 31 includes a stirring mechanism 35 that stirs the reaction liquid in the reaction vessel 20.
  • a plurality of magnetism collecting mechanisms 31a are provided at positions corresponding to the reaction container storage locations.
  • the magnetic flux collecting mechanism 31a is provided at the bottom of the magnetic flux collecting table 31 so as to be close to or in contact with the bottom surface of each of the accommodated reaction vessels 20. Therefore, the magnetic flux collecting table 30 includes two or more magnetic flux collecting mechanisms 31 and can perform a magnetic flux collecting process on the two or more reaction vessels 20 in parallel.
  • FIG. 3 is a diagram for explaining the reaction between the measurement target in the specimen and the magnetic particles 61 and the labeling particles 62 contained in the reagent.
  • the state in the reaction container 20 transferred to the magnetic collection table 31 will be described.
  • the sample in the sample container 21a moved to the suction position of the sample transfer lane 21 is injected into the reaction container 20 in the magnetic collection table 31 by the sample dispensing unit 22, and as shown in FIG.
  • the first reagent containing the magnetic particles 61 is dispensed by the first reagent dispensing device 27 or 28, and the second reagent containing the gold particles 62 as the labeling substance is dispensed by the second reagent dispensing device 29 or 30.
  • the magnetic particles 61 and the gold particles 62 react with the measurement object 60 in the specimen.
  • FIG. 3 (2) the magnetic particles 61, the gold particles 62, and the measurement object 60 are combined.
  • a body 63 is formed.
  • FIG. 4 is a diagram showing a configuration of the magnetic flux collecting mechanism 31a shown in FIG.
  • the magnetism collecting mechanism 31a has, for example, a shape in which a cone is integrally formed on a cylinder.
  • the apex of the conical portion functions as a magnetic pole.
  • the apex of the conical portion of the magnetism collecting mechanism 31 a is provided so as to be close to or in contact with the bottom surface of the magnetism collecting table 31.
  • the magnetic flux collecting mechanism 31a is not limited to a shape in which a cone is integrally formed on a cylinder, but may be a shape in which a cone shape is integrally formed on a prism.
  • the magnetic force is applied from the magnetic collection mechanism 31 a that is close to or in contact with the bottom surface of the reaction vessel 20 when the composite 63 is conveyed to the magnetic collection position of the magnetic collection table 31. It is attracted in response.
  • the composite 63 gradually aggregates on the bottom surface of the reaction container 20 so as to be close to the tip of the conical portion of the magnetic flux collecting mechanism 31 a.
  • an aggregate 64 is formed in the reaction vessel 20 after a predetermined time has elapsed.
  • the magnetic flux collecting mechanism 31 a forms the aggregates 64 on the bottom surface of the reaction vessel 20 by bringing the magnetic poles close to or in contact with the bottom surface of the reaction vessel 20.
  • the magnetic force of the magnetic flux collecting mechanism 31a is set corresponding to the aggregate diameter serving as a target.
  • the diameter of the aggregate is set to, for example, 200 ⁇ m or more and 2000 ⁇ m or less, 10 ⁇ m or more and 1000 ⁇ m or less, 50 ⁇ m or more and 500 ⁇ m or less, or 100 ⁇ m or more and 500 ⁇ m or less, depending on the spot diameter of laser light in the photometry unit 34 described later.
  • the apex angle ⁇ at the tip of the conical portion of the magnetic flux collecting mechanism 31a needs to be 90 ° or less, and more preferably 60 ° or less so that the formation of aggregates proceeds smoothly.
  • the second container transfer unit 33 performs photometry of the reaction container 20 in which the magnetic collection process is performed by the magnetic collection table 31 and the aggregate 64 is formed from the take-out position of the magnetic collection table 31 by the photometry unit 34 at a predetermined timing. Move to position.
  • the second container transfer unit 33 has the same configuration as that of the first container transfer unit 32 and includes an arm having a gripping device that can grip the reaction container 20 at the tip.
  • the second container transfer unit 33 moves the reaction container 20 from the magnetism collecting table 31 to the photometry unit 34 by moving the arm up and down in the vertical direction by a driving means such as a stepping motor and rotating its base end as a rotation axis. Transport.
  • the photometric unit 34 performs photometric processing on the aggregate in the reaction vessel 20 that has been subjected to the magnetic flux collection process by the magnetic flux collecting mechanism 31 a and has been transferred by the second vessel transfer unit 33, and the aggregate 64. Measure the optical properties of The photometry unit 34 measures the optical characteristics of the aggregate 64 in a state in which a complex of the magnetic particles, the measurement object, and the label particles is aggregated in the reaction container 20 by the magnetic flux collection process.
  • FIG. 5 is a diagram showing the configuration of the photometry unit shown in FIG. As shown in FIG. 5, the photometry unit 34 includes a laser light source 34a, lenses 34b, 34d, and 34e, a dichroic mirror 34c, and a Raman spectrometer 34f.
  • the laser light emitted from the laser light source 34a is converged into parallel light by the lens 34b, reflected by the dichroic mirror 34c, and then collected by the lens 34d and incident on the aggregate 64, as indicated by the optical path L1.
  • the Raman scattered light whose surface is enhanced by the aggregate 64 is converged to parallel light by the lens 34d, passes through the dichroic mirror 34c, and is then collected by the lens 34e, and is subjected to the Raman spectrometer 34f. Is incident on.
  • the measurement result of the Raman spectrometer 34 f is output to the control unit 41 and analyzed by the analysis unit 43.
  • the reaction container 20 that has been subjected to photometric processing by the photometric unit 34 is taken out of the photometric unit 34 by a transfer mechanism (not shown) and discarded.
  • the control mechanism 4 includes a control unit 41, an input unit 42, an analysis unit 43, a storage unit 44, an output unit 45, and a dispensing accuracy management unit 46. These units included in the measurement mechanism 2 and the control mechanism 4 are electrically connected to the control unit 41.
  • the control unit 41 is configured using a CPU or the like, and controls the processing and operation of each unit of the analyzer 1.
  • the control unit 41 performs predetermined input / output control on information input / output to / from each of these components, and performs predetermined information processing on this information.
  • the input unit 42 is configured by using a keyboard, a mouse, and the like, and acquires various information necessary for analysis of the specimen, instruction information for analysis operation, and the like from the outside.
  • the analysis unit 43 analyzes the sample based on the Raman spectroscopic analysis result acquired from the photometry unit 34.
  • the storage unit 44 is configured by using a hard disk that magnetically stores information and a memory that electrically loads various programs related to the process from the hard disk when the analyzer 1 executes the process, Various information including the analysis result of the sample is stored.
  • the storage unit 44 may include an auxiliary storage device that can read information from a storage medium such as a CD-ROM, a DVD-ROM, or a PC card.
  • the storage unit 44 also stores a calibration curve for each Raman reagent for dispensing accuracy management of a reagent dispensing unit described later.
  • the output unit 45 is configured using a printer, a speaker, and the like, and outputs various information including the analysis result of the sample.
  • the output unit 45 may output information according to a predetermined format to an external device via a communication network (not shown).
  • the determination unit 46c of the dispensing management unit 46 determines that the dispensing amount of the reagent probe is outside the predetermined range
  • the output unit 45 indicates that the dispensing probe has an abnormal dispensing amount for the reagent probe that is outside the predetermined range. Output an alert.
  • the dispensing accuracy management unit 46 includes a dispensing control unit 46a, a calculation unit 46b, and a determination unit 46c.
  • the dispensing control unit 46a is configured so that the first reagent dispensing units 27 and 28 and the second reagent dispensing units 29 and 30 are used in the first reaction container 20 at a predetermined timing or timing designated by the operator.
  • Different Raman reagents accommodated in the Raman reagent containers 25b and 26b in the reagent container 25 and the second reagent container 26 are respectively dispensed, and after stirring the dispensed Raman reagent in the reaction container 20, the photometry unit 34
  • Each Raman reagent is subjected to photometric analysis, and the analysis unit 43 is controlled to analyze the concentration of each Raman reagent.
  • the Raman reagent used for the dispensing accuracy control is obtained by modifying a core active nanoparticle (including nanocolloids, nanorods, beads, GAN particles) with a Raman active molecule.
  • the modification means a state in which the core nanoparticle and the Raman active molecule are bonded, or a state in which the Raman active molecule is associated with the core nanoparticle.
  • nanoparticles gold, silver, copper or platinum particles, or silver / gold, platinum / gold, copper / gold alloy, etc. are used, and gold nanoparticles are particularly preferable in terms of stability.
  • the Raman active molecule refers to a substance capable of Raman-shifting the scattered light of the core nanoparticle by modifying the core nanoparticle, such as a polycyclic aromatic compound or a heterocyclic aromatic compound.
  • a polycyclic aromatic compound or a heterocyclic aromatic compound preferably benzotriazole, trans-1- (2-pyridyl) -2- (4-pyridyl) ethylene, 1,2-di (4-dipyridyl) ethylene, 2-iminothiolane, 2-naphthalenethiol, rhodamine 6G , Pyridine, 4-mercaptobenzoic acid, 3-mercaptobenzoic acid, thiophenol, 4-aminobenzenethiol, 2-aminobenzenethiol, benzenethiol, octadecanthyl, 11-amino-1-undecanthyl.
  • FIG. 6 is a diagram showing the Raman shift of each Raman reagent. 6 (6-1) is the Raman shift of Raman reagent A, FIG. 6 (6-2) is the Raman shift of Raman reagent B, FIG. 6 (6-3) is the Raman shift of Raman reagent C, and FIG. 4) shows the Raman shift of the Raman reagent D.
  • FIG. 6 (6-1) is the Raman shift of Raman reagent A
  • FIG. 6 (6-2) is the Raman shift of Raman reagent B
  • FIG. 6 (6-3) is the Raman shift of Raman reagent C
  • FIG. 4 shows the Raman shift of the Raman reagent D.
  • FIG. 6 (6-5) shows the Raman shift of the mixed solution of reagents A, B, C and D.
  • the Raman shift of each Raman reagent differs in the position of the main peak as shown in FIG. Therefore, even if each Raman reagent is dispensed into the same reaction vessel 20, as shown in FIG. 6 (6-5), the position of the main peak does not overlap, so that multiple analysis can be performed at one time. The time required for checking the dispensing accuracy can be greatly reduced.
  • the Raman reagent used in the first embodiment may be a nanoparticle modified with a Raman active molecule.
  • the spectral intensity of the Raman reagent is proportional to its concentration, a small amount of Raman reagent is used.
  • the Raman reagent and the magnetic particle are combined (hereinafter, the combination of the Raman reagent and the magnetic particle is referred to as “Raman standard particle”), and the Raman scattered light analysis is performed after collecting the magnetic flux. Therefore, it is possible to improve the analysis accuracy with a small amount of Raman standard particles.
  • FIG. 7 is a schematic diagram illustrating a method for generating Raman standard particles according to the first embodiment of the present invention.
  • At least one Raman-active molecule 72 is brought into contact with the metal particles 71 to cause a reaction.
  • the Raman active molecule 72 is adsorbed on the surface of the metal particle 71.
  • complex 73 which is SERS particle
  • the metal particles 71 are gold particles, silver particles, copper particles, or platinum particles, and are particles that cause surface plasmon enhancement.
  • the Raman active molecule 72 is 4-mercaptobenzoic acid, 3-mercaptobenzoic acid, 4-aminobenzenethiol, 2-aminobenzenethiol, benzenethiol, octadecanthyl, 11-amino-1-undecanthyl, etc., and metal particles 71 Produces a characteristic Raman peak.
  • the first composite 73 is reacted with the cross-linking agent 74 in contact with the condensing agent (FIG. 7C).
  • the crosslinking agent 74 is covalently bonded to the carboxyl group or thiol group of the Raman active molecule 72 in the presence of the condensing agent.
  • complex 73 is produced
  • the condensing agent is N- (3-dimethylaminopropyl) -N′-ethylcarbodiimide (EDC, WSC), 1- (3-dimethylaminopropyl) -3-ethyl (EDC method), N ′-( Ethylcarbonimidoyl) -N, N-dimethyl-1,3-polopandiamine / hydrochloric acid (EDC hydrochloride, EDAC), N, N′-diisopropylcarbodiimide (DIC), 1-ethyl-3-tert-butylcarbodiimide (BEC) ), 1-cyclohexyl-3- (2-morpholinoethyl) carbodiimide-p-toluenesulfonate (CMC), N, N′-di-tert-butylmethanediimine, 1,3-bis (p-tolyl) carbodiimide Or, N, N′-dicyclo
  • the magnetic particles 76 are brought into contact with the second complex 75 to be reacted (FIG. 7 (e)). Specifically, the amino group of the crosslinking agent 74 of the second complex 75 is reacted to covalently bond the magnetic particle 76 having a tosyl group as a surface functional group. As a result, Raman standard particles 77 in which the magnetic particles 76 are covalently bonded to the second composite 75 are generated (FIG. 7F).
  • the magnetic particle 76 is a particle whose core is ferrite and whose core is covered with resin or silica.
  • the magnetic particle 76 may be a particle whose core is resin or silica and whose core is covered with ferrite.
  • the calculation unit 46b is measured by the photometry unit 34 under the control of the dispensing control unit 46a, and the first reagent dispensing units 27 and 28 are determined from the concentrations of the Raman reagents or Raman standard particles obtained by the analyzing unit 43. And the dispensing amount of the Raman reagent actually dispensed from each reagent probe of the second reagent dispensing units 29 and 30 is calculated.
  • the determination unit 46c determines whether or not the dispensing amount of each reagent probe calculated by the calculation unit 46b is within a predetermined range.
  • the sample dispensing unit 22 dispenses the sample in the sample container 21a to the reaction containers 20 that are sequentially transported, and the first reagent dispensing unit 27 or 28 is dispensed. Dispenses the first reagent in the first reagent container 25a, then the second reagent dispensing part 29 or 30 dispenses the second reagent in the second reagent container 26a and is stirred, and then the magnetic collecting table After the magnetic flux collection process is carried to 31, the photometric unit 34 performs Raman scattered light measurement of the formed aggregate 64 obtained by reacting the sample and the reagent, and the analysis unit 43 analyzes the measurement result, whereby the sample The antigen-antibody reaction analysis is automatically performed.
  • FIG. 8 is a diagram showing the dispensing operation of the Raman reagent by each reagent probe.
  • the dispensing accuracy management unit 46 receives a timing for dispensing accuracy management of the reagent dispensing unit or an instruction to confirm the dispensing accuracy of the operator.
  • the first reagent dispensing units 27 and 28 and the second reagent dispensing units 29 and 30 are controlled to dispense a predetermined amount of different Raman reagents into the same reaction vessel 20.
  • the dispensing control unit 46a of the dispensing accuracy management unit 46 uses the dispensing probe 27a of the first reagent dispensing unit 27 to store the Raman reagent A stored in the Raman reagent container 25b in the first reagent storage 25. A predetermined amount is dispensed into the reaction vessel 20 (see FIG. 8 (8-1)).
  • the dispensing control unit 46a uses the dispensing probe 28a of the first reagent dispensing unit 28 to transfer the Raman reagent B contained in another Raman reagent container 25b in the first reagent storage 25 to the dispensing probe 28a.
  • a predetermined amount is dispensed into the reaction container 20 into which the Raman reagent A has been dispensed (see FIG. 8 (8-2)).
  • the dispensing control unit 46a uses the dispensing probe 29a of the second reagent dispensing unit 29 to supply the Raman reagent C stored in the Raman reagent container 26b in the second reagent storage 26 with the dispensing probe 29a.
  • a predetermined amount is dispensed into the reaction vessel 20 into which reagents A and B have been dispensed (see FIG. 8 (8-3)).
  • the dispensing control unit 46a uses the dispensing probe 30a of the second reagent dispensing unit 30 to transfer the Raman reagent D stored in another Raman reagent container 26b in the second reagent storage 26 using the dispensing probe 30a.
  • a predetermined amount is dispensed into the reaction vessel 20 into which the Raman reagents A, B, and C have been dispensed (see FIG. 8 (8-4)).
  • the Raman reagents A, B, C and D dispensed in the reaction vessel 20 (see FIG.
  • the calculation unit 46b calculates the dispensing amount of each dispensing probe based on the concentration of the Raman reagents A, B, C and D, The dispensing accuracy of each dispensing probe can be confirmed.
  • Raman standard particles are used instead of the Raman reagent, the mixture is stirred by the stirring mechanism 35 and collected by the magnetism collecting mechanism 31a, and then the Raman scattered light analysis is performed by the photometric unit 34.
  • FIG. 9 is a flowchart of the dispensing accuracy management process according to the first embodiment.
  • the dispensing control unit 46a firstly dispenses the dispensing probe of the first reagent dispensing unit 27.
  • the Raman reagent A accommodated in the reagent container 25b in the first reagent container 25 is aspirated (step S101) and controlled to be discharged to the reaction container 20 on the reaction container transfer lane 24 (step S102).
  • the dispensing control unit 46a checks whether or not the dispensing of the Raman reagent by all the reagent dispensing probes provided in the automatic analyzer 1 has been completed (step S103). In the first embodiment, as shown in FIG. 7, the case where the number of reagent dispensing probes is four will be described. Yes.
  • Step S101 and Step S102 are repeated, and the dispensing control unit 46a dispenses the Raman reagent by all the reagent dispensing probes.
  • the first container transfer unit 32 transfers the reaction container 20 from the reaction container transfer lane 24 to the magnetic collection table 31. After the reaction container 20 is transferred to the magnetic collection table 31, the Raman reagent in the reaction container 20 is stirred by the stirring mechanism 35 (step S104).
  • the second container transfer unit 33 transfers the reaction container 20 from the magnetic collection table to the photometry unit 34, and then the photometry unit 34 performs Raman scattered light analysis on the Raman reagents A, B, C, and D in the reaction container 20. Is performed (step S105).
  • the analysis unit 43 analyzes the concentrations of the Raman reagents A, B, C, and D based on the Raman photometric analysis data measured by the photometry unit (step S106). Subsequently, the calculation unit 46b calculates the dispensing amounts of the reagent probes 27a, 28a, 29a, and 30a from the concentrations of the Raman reagents A, B, C, and D (step S107).
  • the determination unit 46c determines whether or not the dispensing amounts of the Raman reagents A, B, C, and D dispensed from the reagent probes 27a, 28a, 29a, and 30a calculated by the calculation unit 46b are within a predetermined range. (Step S108). When the dispensing amount of all the Raman reagents is within the predetermined range (step S108, Yes), the dispensing accuracy management process is terminated. On the other hand, when there is a dispensing amount of the Raman reagent outside the predetermined range (step S108, No), the first and / or second reagent dispensing including the reagent probe under dispensing the Raman reagent outside the predetermined range. An alert to the effect that the dispensing accuracy is out of range is output by the output unit 45 (step S109), and the dispensing accuracy management process is terminated.
  • multiple Raman measurement can be performed by dispensing different Raman reagents with a plurality of reagent dispensing units and performing Raman scattered light analysis. It is possible to manage the accuracy of a plurality of reagent dispensing sections by analyzing the degree. Thereby, it is possible to significantly reduce the time required for maintenance of the reagent dispensing unit.
  • the automatic analyzer 1A dispenses the same amount of different Raman reagents with one dispensing probe, analyzes the concentration of each Raman reagent with the photometric unit 34, and dispenses each Raman reagent.
  • the dispensing accuracy of the dispensing probe is confirmed by calculating the amount and the coefficient of variation of the dispensing amount.
  • FIG. 10 is a schematic diagram showing the configuration of the automatic analyzer 1A according to the second embodiment of the present invention.
  • the automatic analyzer 1A is different from the automatic analyzer 1 of the first embodiment in that it includes a dispensing accuracy management unit 47.
  • the dispensing accuracy management unit 47 of the second embodiment includes a dispensing control unit 47a, a calculation unit 47b, and a determination unit 47c, and includes first reagent dispensing units 27 and 28, second included in the automatic analyzer 1A. The dispensing accuracy of any one dispensing probe selected from the reagent dispensing units 29 and 30 is confirmed.
  • the dispensing control unit 47a uses the dispensing probe instructed by the operator or the dispensing probe in a predetermined order at a predetermined timing or a timing instructed by the operator to place the first reagent container in one reaction container 20. 25 or the different Raman reagents contained in the Raman reagent containers 25b and 26b in the second reagent storage 26, respectively, and after stirring the dispensed Raman reagent in the reaction container 20, the photometric unit 34 uses the Raman reagents to stir. The reagent is subjected to photometric analysis, and the analysis unit 43 is controlled to analyze the concentration of each Raman reagent.
  • the calculating unit 47b calculates the dispensing amount of each dispensing probe from the concentration of each Raman reagent obtained by the analyzing unit 43, and calculates the variation coefficient of the dispensing amount.
  • the determination unit 47c determines whether or not the variation coefficient calculated by the calculation unit 47b is equal to or less than a predetermined value.
  • FIG. 11 is a diagram illustrating the dispensing operation of the Raman reagent by the designated dispensing probe according to the second embodiment.
  • the dispensing accuracy management unit 47 receives a timing for dispensing accuracy management of the reagent dispensing unit or an instruction to confirm the dispensing accuracy of the operator.
  • a predetermined amount of different Raman reagents are dispensed into the same reaction vessel 20 by a dispensing probe instructed by the operator or a dispensing probe in a predetermined order.
  • the dispensing controller 47a causes the dispensing reagent 27a to dispense a predetermined amount of the Raman reagent A accommodated in the Raman reagent container 25b in the first reagent storage 25 into the reaction container 20 (FIG. 11 (11). See -1)).
  • the dispensing controller 47a similarly reacts the Raman reagent B accommodated in another Raman reagent container 25b in the first reagent container 25 with the dispensing probe 27a.
  • a predetermined amount is dispensed into the container 20 (see FIG. 11 (11-2)).
  • Embodiment 2 since different Raman reagents are dispensed by one dispensing probe 27a, it is preferable to wash the dispensing probe 27a between dispensing. Further, in order to manage the dispensing accuracy by the coefficient of variation, the dispensing amount of each Raman reagent is set to the same amount.
  • the Raman reagents A, B, C, D, and E dispensed into the reaction vessel 20 (see FIG. 11 (11-6)), after stirring with the stirring mechanism 35, Raman scattering light analysis was performed with the photometric unit 34, After analyzing the concentrations of the Raman reagents A, B, C, D and E by the analysis unit 43, the calculation unit 47b uses the Raman probes A, B, C, D and E based on the concentrations of the Raman reagents A, B, C, D and E. The dispensing accuracy of each dispensing probe is confirmed by calculating the dispensing amount and the variation coefficient of the dispensing amount.
  • FIG. 12 is a flowchart of the dispensing accuracy management process according to the second embodiment.
  • the dispensing controller 47a aspirates the Raman reagent A stored in the Raman reagent container 25b in the first reagent storage 25 by the dispensing probe 27a of the first reagent dispensing unit 27 (Step S201). Control is performed so as to discharge to the reaction vessel 20 on the vessel transfer lane 24 (step S202).
  • step S201 and step S202 are repeated, and the dispensing control unit 47a causes the set number of times of dispensing of the Raman reagent by the dispensing probe 27a.
  • step S203 Yes
  • the first container transfer unit 32 transfers the reaction container 20 from the reaction container transfer lane 24 to the magnetism collecting table 31. After the reaction container 20 is transferred to the magnetic collection table 31, the Raman reagent in the reaction container 20 is stirred by the stirring mechanism 35 (step S204).
  • the second container transfer unit 33 transfers the reaction container 20 from the magnetism collecting table to the photometry unit 34, and then the photometry unit 34 performs Raman scattered light on the Raman reagents A, B, C, D, and E in the reaction container 20.
  • Analysis is performed (step S205).
  • the analysis unit 43 analyzes the concentrations of the Raman reagents A, B, C, D, and E based on the Raman scattered light data measured by the photometry unit (step S206).
  • the calculation unit 47b calculates the dispensing amount of each Raman reagent A, B, C, D, and E by the reagent probe 27a from the concentration of each Raman reagent A, B, C, D, and E, and the variation of the dispensing amount.
  • a coefficient is calculated (step S207).
  • the determination unit 47c determines whether or not the variation coefficient of the predetermined dispensing amount dispensed by the reagent probe 27a calculated by the calculation unit 47b is equal to or less than a predetermined value (step S208). When it is below the predetermined value (step S208, Yes), the dispensing accuracy management process is terminated. If it is larger than the predetermined value (No at Step S208), an alert that the dispensing accuracy is out of the range for the first reagent dispensing unit 27 including the reagent probe 27a is output by the output unit 45 (Step S209). The dispensing accuracy management process is terminated.
  • the dispensing accuracy management since different Raman reagents are dispensed by one dispensing unit and Raman scattered light analysis is performed, multiple measurements can be performed. It is possible to perform accuracy management based on the variation coefficient of the dispensed amount in the predetermined dispensing unit with one analysis, and it is possible to greatly reduce the time required for maintenance of the dispensing unit.
  • the second embodiment it is possible to manage the accuracy of the dispensing probe of the sample dispensing unit 22 as well as the first and second reagent dispensing units.
  • a Raman reagent container that accommodates the Raman reagent in a shape that can be transferred in the sample transfer lane 21 is installed in the sample transfer lane 21, and the Raman reagent container The analysis may be performed by aspirating the Raman reagent.
  • the same amount of different Raman reagents are dispensed into the reaction vessel 20 by one dispensing probe, and the concentration of each Raman reagent is measured by the photometric unit 34.
  • the calculation unit 47b calculates the average dispensing amount of each Raman reagent and confirms the dispensing accuracy of the dispensing probe based on whether or not the average dispensing amount is within a predetermined range. Is done.
  • the calculation unit 47b calculates both the average dispensing amount and the coefficient of variation based on the analyzed concentration of each Raman reagent, and when either the average dispensing amount or the variation coefficient is out of the range, the output unit 45 It is good also as issuing an alert.
  • the automatic analyzer 1B As in the first embodiment, the automatic analyzer 1B according to the third embodiment dispenses different Raman reagents with different reagent probes, and the photometric unit 34 analyzes the concentration of each Raman reagent a plurality of times. By repeating, the average dispensing amount and the variation coefficient of each dispensing amount are calculated for each reagent probe, and the dispensing accuracy of each reagent probe is confirmed.
  • FIG. 13 is a schematic diagram showing the configuration of the automatic analyzer 1B according to the third embodiment of the present invention.
  • the automatic analyzer 1B is different from the automatic analyzer 1 of the first embodiment in that it includes a dispensing accuracy management unit 48.
  • the dispensing accuracy management unit 48 includes a dispensing control unit 48a, a calculation unit 48b, and a determination unit 48c.
  • the dispensing control unit 48a uses the dispensing probes 27a, 28a, 29a and 30a at the predetermined timing or the timing designated by the operator to place the first reagent container 25 or the second reagent container 26 in the first reaction container 20.
  • the different Raman reagents accommodated in the reagent containers 25b and 26b are respectively dispensed, and after stirring the dispensed Raman reagent in the reaction container 20, the Raman reagent is photometrically analyzed by the photometric unit 34, and the analyzing unit 43 Control to analyze the concentration of each Raman reagent.
  • the dispensing control unit 48a repeats the dispensing of the Raman reagent by the dispensing probes 27a, 28a, 29a and 30a and the analysis by the photometric unit 34 a predetermined number of times.
  • the dispensing amounts of the Raman reagents of the dispensing probes 27a, 28a, 29a, and 30a may be different, but the dispensing amount of each dispensing probe that is repeated a predetermined number of times depends on the average dispensing amount and the coefficient of variation. Same amount for calculation.
  • the calculation unit 48b calculates the dispensing amount of each of the dispensing probes 27a, 28a, 29a, and 30a from the concentration of each Raman reagent obtained by the analysis unit 43, and calculates the average dispensing amount and the dispensing from the dispensing amount. The coefficient of variation of quantity is calculated.
  • the determination unit 48c determines whether the average dispensing amount calculated by the calculation unit 48b is within a predetermined range and whether the variation coefficient is equal to or less than a predetermined value.
  • FIG. 14 is a flowchart of the dispensing accuracy management process according to the third embodiment.
  • the dispensing controller 48a aspirates the Raman reagent A accommodated in the Raman reagent container 25b in the first reagent storage 25 by the dispensing probe 27a of the first reagent dispensing unit 27 (step S401), and reacts. Control is performed so as to discharge to the reaction vessel 20 on the vessel transfer lane 24 (step S402).
  • the dispensing control unit 48a checks whether or not the Raman reagent dispensing by all the reagent dispensing probes provided in the automatic analyzer 1B has been completed (step S403).
  • Step S401 and Step S402 are repeated, and the dispensing control unit 48a dispenses the Raman reagent by all the reagent dispensing probes.
  • the first container transfer unit 32 transfers the reaction container 20 from the reaction container transfer lane 24 to the magnetism collection table 31. After the reaction container 20 is transferred to the magnetic collection table 31, the Raman reagent in the reaction container 20 is stirred by the stirring mechanism 35 (step S404).
  • the second container transfer unit 33 transfers the reaction vessel 20 from the magnetic collection table to the photometry unit 34, and then the photometry unit 34 performs Raman scattered light analysis on the Raman reagents A, B, C, and D in the reaction vessel 20. This is performed (step S405).
  • the analysis unit 43 analyzes the concentrations of the Raman reagents A, B, C, and D based on the Raman scattered light analysis data measured by the photometry unit (step S406).
  • the dispensing control unit 48a checks whether or not the analysis by the photometry unit 34 has been performed a predetermined number of times (n times) (step S407). If the dispensing control unit 48a determines that the Raman reagent dispensing and photometric processing has not been performed a predetermined number of times (n times) (No in step S407), steps S401 to S406 are repeated. When the dispensing control unit 48a determines that the dispensing of the Raman reagent and the photometric process have been performed a predetermined number of times (n times) (Yes in step S407), the calculating unit 48b calculates the Raman reagent A, B, C, and D. From the concentration (n times), the average dispensing amount and coefficient of variation of the reagent probes 27a, 28a, 29a and 30a are calculated (step S408).
  • the determination unit 48c determines whether the average dispensing amount of the Raman reagents A, B, C, and D dispensed from the reagent probes 27a, 28a, 29a, and 30a calculated by the calculation unit 48b is within a predetermined range. Determination is made (step S409). When it is within the predetermined range (step S409, Yes), it is determined whether or not the variation coefficient calculated by the calculation unit 48b is equal to or less than a predetermined value (step S411). When it is outside the predetermined range in step S409 (step S409, No), the output unit 45 outputs an alert that the dispensing accuracy is out of the range for the reagent dispensing unit including the reagent probe that is out of the predetermined range.
  • Step S410 If it is less than or equal to the predetermined value in step S411 (step S411, Yes), the dispensing accuracy management process is terminated. If it is larger than the predetermined value (step S411, No), the reagent having a reagent probe larger than the predetermined value is provided. An alert to the effect that the dispensing accuracy is out of range for the dispensing unit is output by the output unit 45 (step S412), and the dispensing accuracy management process is terminated.
  • the automatic analyzer 1C changes the dispensing amount of different Raman reagents by using one dispensing probe, and dispenses each Raman by the photometric unit 34.
  • the reagent concentration By analyzing the reagent concentration and calculating the dispensed amount of each Raman reagent, it is possible to determine whether the relationship between each dispensed amount and the planned dispensed amount is kept linear, thereby dispensing the dispensing probe. Check the accuracy.
  • FIG. 15 is a schematic diagram showing a configuration of an automatic analyzer 1C according to the fourth embodiment of the present invention.
  • the automatic analyzer 1C is different from the automatic analyzer 1 of the first embodiment in that it includes a dispensing accuracy management unit 49.
  • the dispensing accuracy management unit 49 includes a dispensing control unit 49a, a calculation unit 49b, and a determination unit 49c.
  • the first reagent dispensing units 27 and 28, the second reagent dispensing unit 29, and The dispensing accuracy is confirmed by the linearity of the dispensing amount of any one of the 30 dispensing probes 27a, 28a, 29a and 30a.
  • the dispensing controller 49a uses the dispensing probe instructed by the operator or the dispensing probe in a predetermined order at a predetermined timing or a timing instructed by the operator to place the first reagent container in one reaction container 20.
  • 25 or different Raman reagents accommodated in the Raman reagent containers 25b and 26b in the second reagent storage 26 are respectively dispensed by changing the dispensing amount, and after stirring the Raman reagent in the dispensed reaction container 20
  • the photometric unit 34 performs photometric analysis of each Raman reagent, and the analysis unit 43 controls the concentration of each Raman reagent to be analyzed.
  • the calculating unit 49b calculates the dispensing amount of each dispensing probe from the concentration of each Raman reagent obtained by the analyzing unit 43.
  • the determination unit 49c determines whether or not each dispensing amount calculated by the calculation unit 49b maintains linearity.
  • FIG. 16 is a flowchart of dispensing accuracy management according to the fourth embodiment.
  • the dispensing control unit 49a aspirates a predetermined amount of the Raman reagent A stored in the Raman reagent container 25b in the first reagent storage 25 by using a designated or predetermined dispensing probe (step S501), and the reaction container Control is performed to discharge the reaction container 20 on the transfer lane 24 (step S502).
  • the dispensing control unit 49a checks whether or not the dispensing probe has finished dispensing all the set dispensing amounts of the Raman reagent (step S503).
  • at least three or more dispensing amounts are selected, but it is preferable to confirm with five or more dispensing amounts.
  • Steps S501 and S502 are repeated until dispensing of all set dispensing amounts of the Raman reagent is completed (No in step S503), and the dispensing control unit 49a sets all of the set dispensing amounts.
  • the first container transfer unit 32 transfers the reaction container 20 from the reaction container transfer lane 24 to the magnetism collecting table 31. After the reaction container 20 is transferred to the magnetic collection table 31, the Raman reagent in the reaction container 20 is stirred by the stirring mechanism 35 (step S504).
  • the second container transfer unit 33 transfers the reaction container 20 from the magnetism collecting table to the photometry unit 34, and then the photometry unit 34 performs a Raman scattered light analysis for each Raman reagent in the reaction container 20 (step S505).
  • the analysis unit 43 analyzes the concentration of each Raman reagent based on the Raman photometric analysis data measured by the photometry unit (step S506).
  • the calculation unit 49b calculates the dispensing amount of each Raman reagent by the dispensing probe from the concentration of each Raman reagent (step S507).
  • the determining unit 49c determines whether or not the relationship between each dispensing amount of the dispensing probe calculated by the calculating unit 49b and the planned dispensing amount maintains linearity (step S508).
  • the relationship between each dispensed amount and the planned dispensed amount deviates from linearity (step S508, Yes)
  • an alert is output by the output unit for the dispensed amount in which the difference is recognized.
  • an alert that the dispensing accuracy is out of the range may be output by the output unit 45 for the dispensing probe (step S509).
  • the reagent dispensing unit not only the reagent dispensing unit but also the linearity of the dispensing amount of the dispensing probe of the sample dispensing unit 22 can be determined and the accuracy can be managed.
  • a Raman reagent container that accommodates the Raman reagent in a shape that can be transported in the sample transport lane is installed in the sample transport lane 21, and the Raman reagent container is subjected to Raman.
  • a Raman analysis may be performed by aspirating the reagent.
  • the dispensing amount of each dispensing unit is determined using an analysis kit in which a predetermined amount of each Raman reagent used for dispensing accuracy management in an automatic analyzer is housed in an individual reaction vessel 20. It is an automatic analyzer for correction.
  • FIG. 17 is a schematic diagram showing a configuration of an automatic analyzer 1D according to the fifth embodiment of the present invention.
  • the automatic analyzer 1D is different from the automatic analyzer 1 of the first embodiment in that it includes a dispensing accuracy management unit 50.
  • the dispensing accuracy management unit 50 includes a dispensing control unit 50a, a calculation unit 40b, a determination unit 50c, and a correction unit 50d, and includes first reagent dispensing units 27 and 28, a second reagent.
  • the dispensing accuracy is managed based on the dispensing amount of the dispensing probes 27a, 28a, 29a, and 30a of the dispensing units 29 and 30 or the variation coefficient of the dispensing amount.
  • the accuracy of analysis can be improved by correcting each dispensing unit based on the above.
  • the dispensing control unit 50a performs the first reagent dispensing unit 27 and 28 and the second reagent dispensing unit 29 and 30 in the first reaction container 20 at a predetermined timing or timing designated by the operator.
  • Different Raman reagents accommodated in the Raman reagent containers 25b and 26b in the reagent container 25 and the second reagent container 26 are respectively dispensed, and after stirring the dispensed Raman reagent in the reaction container 20, the photometry unit 34 Control to measure the concentration of each Raman reagent.
  • the dispensing control unit 50a conveys the reaction vessel 20 installed in the reaction vessel transfer lane 24 to the photometry unit 34 for the reaction vessel 20 that stores a predetermined amount of different Raman reagents constituting the analysis kit 100, respectively.
  • the photometric unit 34 performs Raman scattered light analysis, and the analysis unit 43 controls to analyze the concentration of each Raman reagent.
  • the analysis kit 100 used in the fifth embodiment includes a reaction container 20 that contains a predetermined amount of Raman reagents A, B, C, and D used as dispensing accuracy confirmation reagents.
  • the upper part of each reaction vessel 20 is sealed with a lid or a seal.
  • the calculation unit 50b and the determination unit 50c play the same role as in the first embodiment.
  • the correction unit 50d calculates the calibration curve of each Raman reagent A, B, C, D stored in the storage unit 44d. to correct.
  • FIG. 19 is a flowchart of the dispensing unit correction process according to the fifth embodiment.
  • FIG. 20 shows a flowchart of the analysis kit analysis process of FIG.
  • the dispensing control unit 50a performs the analysis process of the analysis kit 100 (step S601).
  • the first container transfer unit 32 and the first container transfer unit 33 set the reaction container 20 containing the Raman reagent to the photometric unit 34.
  • the photometric unit 34 performs Raman photometric analysis (step S702).
  • the dispensing control unit 50a checks whether the analysis has been completed for all the reaction containers 20 of the analysis kit 100 (step S703), and if not completed (step S703, No), the Raman reagents A, B, C , Step S701 and Step S702 are repeated until the analysis is completed for the reaction containers 20 that respectively accommodate D.
  • step S703, Yes the process proceeds to step S602 in FIG.
  • the analysis kit 100 can be used repeatedly by storing it in a cool and dark place without discarding it after the photometric process by the photometric unit 34.
  • steps S101 to S109 (see FIG. 9) described in the first embodiment are performed (step S602).
  • the dispensing control unit 50a checks whether there is a reagent probe determined to be dispensing abnormality by the determination unit 46c (step S603), and is determined to be dispensing abnormality. If a reagent probe is present (step S603, Yes), a Raman reagent calibration curve is generated based on the analysis result of the analysis kit 100 analyzed in step S601 for the Raman reagent for dispensing accuracy management of the dispensing probe. Correction is performed (step S604). If there is no reagent probe determined to be dispensing abnormality (step S603, No), the dispensing accuracy management process according to the fifth embodiment is terminated.
  • the automatic analyzer 1D can not only notify the reagent dispensing unit of dispensing abnormality by the output unit 45, but also can perform first and / or second of dispensing abnormality based on the analysis result of the analysis kit 100. By correcting the calibration curve for the reagent dispensing unit, the analysis can be continued without being interrupted by the maintenance process.
  • the analysis kit 100 is preferably a combination of each Raman reagent contained in a separate reaction vessel 20, but a solution containing a Raman reagent mixture having a known concentration of each Raman reagent contained in one reaction vessel 20. There may be.
  • calibration curve correction processing using the analysis kit for dispensing accuracy management processing of the fourth embodiment is illustrated.
  • the analysis kit is an analysis kit for confirming the linearity of the dispensed amount, and each reaction container 20 accommodates Raman reagents A, B, C, D, and E having different dispensed amounts.
  • the dispensing accuracy management process of the fourth embodiment is performed, and dispensing that deviates from linearity is performed. Calibration curves can be corrected for quantities.
  • the automatic analyzer according to the sixth embodiment automatically manages the stirring performance of the stirring mechanism in the apparatus using a plurality of different Raman reagents.
  • FIG. 21 is a schematic diagram showing a configuration of an automatic analyzer 1E according to the sixth embodiment of the present invention.
  • the automatic analyzer 1E includes a first reagent container 25e that houses a Raman reagent container 25c that houses a Raman reagent used for the stirring performance management process, and a stirring performance management unit 51.
  • the stirring performance management process of the sixth embodiment uses magnetic particles 61 accommodated in the reagent container 25a as the first reagent and a Raman reagent that specifically reacts with the magnetic particles 61.
  • the Raman reagent used in the stirring performance management process is a unique core and nanoparticle with Raman activity (including nanocolloids, nanorods, beads, and GAN particles) modified with a Raman active molecule. Refers to a further modified target molecule that reacts automatically.
  • the Raman reagent that specifically reacts with the magnetic particles 61 will be described as Raman reagents F and G.
  • the Raman reagents F and G are Raman reagents obtained by modifying Raman active molecules having different main peak positions when measuring Raman scattered light.
  • the stirring performance management unit 51 includes a dispensing control unit 51a, a calculation unit 51b, and a determination unit 51c, and manages the stirring performance of the stirring mechanism 35.
  • the dispensing control unit 51a dispenses the Raman reagents F and G modified with target particles into one reaction vessel 20 at a predetermined timing or a timing instructed by an operator, and reacts with the target molecules. After further dispensing the magnetic particles 61 modified in the above, the mixture is stirred in a predetermined manner and reacted to form a complex, and the magnetic particles 61 in the reaction vessel 20 are moved by the magnetic collecting mechanism 31a installed outside the reaction vessel 20. After collecting the composite, the Raman reagent F and G in the aggregate generated by the magnetic collection is measured by the photometry unit 34, and the analysis 43 is controlled to analyze the concentration of each Raman reagent.
  • the calculation unit 51b calculates the reaction ratio between the magnetic particles 61 and the Raman reagents F and G from the concentrations of the Raman reagents F and G obtained by the analysis unit 43.
  • the determination unit 51c determines whether or not the reaction ratio calculated by the calculation unit 51b is within a predetermined range.
  • FIG. 22 is an operation diagram of the stirring performance management process according to the sixth embodiment.
  • a predetermined amount of Raman reagent F stored in the Raman reagent container 25c is dispensed into the reaction container 20 by the reagent probe 27a or 28a of the first reagent dispensing unit 27 or 28. Note (see Fig. 22 (22-1)). Thereafter, a predetermined amount of the Raman reagent G accommodated in another Raman reagent container 25c is dispensed into the same reaction container 20 by the reagent probe 27a or 28a (see FIG. 22 (22-2)). Further, a predetermined amount of the magnetic particles 61 accommodated in the first reagent container is dispensed by the reagent probe 27a or 28a (see FIG. 22 (22-3)).
  • the dispensing amount of the magnetic particles 61 is not more than the total amount of the Raman reagents F and G.
  • a composite 65 in which the Raman reagent F and the magnetic particles 61 have reacted and a composite 66 in which the Raman reagent G and the magnetic particles 61 have reacted.
  • the generated composites 65 and 66 are magnetized by the magnetism collecting mechanism 31a.
  • the composites 65 and 66 contain magnetic particles, the composites 65 and 66 are attracted by the magnetic force of the magnetic collecting mechanism 31a that is close to or in contact with the bottom surface of the reaction vessel 20 when transported to the magnetic collection position, and gradually move toward the bottom surface of the reaction vessel 20. Aggregates to form aggregates 67 (see FIG. 22 (22-5)). By analyzing the concentration of the Raman reagents F and G in the aggregate 67, the reaction ratio between the magnetic particles 61 and the Raman reagents F and G is calculated, and the stirring performance of the stirring mechanism 35 is managed based on the reaction ratio. To do.
  • FIG. 23 is a flowchart of the stirring performance management process according to the sixth embodiment.
  • the dispensing control unit 51a uses the reagent probe 27a or 28a of the first reagent dispensing unit 27 or 28 to perform the Raman reagent container 25c in the first reagent storage 25.
  • the Raman reagent F stored in the container is aspirated (step S801) and controlled to be discharged into the reaction container 20 on the reaction container transfer lane 24 (step S802).
  • the dispensing control unit 51a checks whether or not dispensing of all the Raman reagents has been completed (step S803).
  • two types of Raman reagents F and G are used, and the reaction ratio between each Raman reagent and the magnetic particles 61 as the first reagent is calculated.
  • Steps S801 and S802 are repeated until the dispensing of all the Raman reagents is completed (No in step S803), and the dispensing control unit 51a confirms that the dispensing of all the Raman reagents is completed (step S803). Yes), the dispensing control unit 51a uses the reagent probe 27a or 28a of the first reagent dispensing unit 27 or 28 to suck the magnetic particles 61 accommodated in the first reagent container 25a in the first reagent storage 25. (Step S804) and control to discharge to the reaction vessel 20 on the reaction vessel transfer lane 24 (Step S805).
  • the Raman reagents F and G and the magnetic particles 61 are dispensed with the same reagent probe, the reagent probe is washed between the dispensing of each reagent.
  • the first container transfer unit 32 transfers the reaction container 20 from the reaction container transfer lane 24 to the magnetism collection table 31.
  • the Raman reagent in the reaction vessel 20 is stirred by the stirring mechanism 35 (step S806), and the magnetic particles 61 and the Raman reagents F and G are reacted for a predetermined time, whereby the composites 65 and 66 are obtained. It is generated (step S807).
  • the composites 65 and 66 generated by the magnetic collecting mechanism 31a are collected at the bottom of the reaction vessel 20 to form an aggregate 67 (step S808).
  • the second container transfer unit 33 transfers the reaction container 20 from the magnetic collection table to the photometry unit 34, and the photometry unit 34 performs Raman scattered light analysis on the aggregate 67 in the reaction container 20 (step S809).
  • the analysis unit 43e analyzes the concentrations of the Raman reagents F and G in the aggregate 67 based on the Raman scattered light analysis data measured by the photometry unit 34 (step S810), and the calculation unit 51b calculates the Raman reagents F and G.
  • the reaction ratio between the magnetic particles 61 and the Raman reagents F and G is calculated from the concentration of (Step S811).
  • the determining unit 51c determines whether or not the reaction ratio of the Raman reagent F and G calculated by the calculating unit 51b with the magnetic particles 61 is within a predetermined range (step S812). When it is within the predetermined range (step S812, Yes), the stirring performance management process is terminated. If it is out of the predetermined range (step S812, No), an alert indicating that the stirring performance of the stirring mechanism 35 is poor is output by the output unit 45 (step S813), and the stirring performance management process is terminated.
  • the automatic analyzer 1E according to the sixth embodiment can automatically manage the stirring performance of the stirring mechanism in the apparatus by a simple method using the reaction between the Raman reagent and the magnetic particles. is there.
  • the dispensing performance management method, the dispensed amount correction method, the stirring performance management method, the automatic analyzer and the analysis kit of the present invention are very useful for reducing the time required for maintenance. Suitable for fields where analysis accuracy is required.

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Abstract

L'invention concerne un procédé de gestion de la précision selon lequel il est possible de réduire fortement le temps d'entretien, y compris la vérification de la précision de distribution. L'invention concerne également un procédé de correction d'un volume distribué, un procédé de gestion de performances d'agitation, un dispositif d'analyse automatique et un kit d'analyse. Spécifiquement, un dispositif d'analyse automatique (1) comprend une unité de commande de la distribution (46a) qui effectue une commande de manière à ce que différents réactifs de Raman soient distribués dans des cuves de réaction (20) du dispositif (1) en utilisant des sondes de distribution, de manière à ce que les réactifs de Raman distribués dans les cuves de réaction (20) soient agités, de manière à ce que les réactifs de Raman soient analysés par photométrie par une unité photométrique (34), et de manière à ce que les réactifs de Raman soient analysés par une unité d'analyse (43) ; une unité de calcul (46b) qui calcule les volumes de distribution des sondes de distribution à partir des densités des réactifs de Raman ; une unité de détermination (46c) qui détermine si une distribution anormale a lieu ou non en fonction des volumes de distribution calculés des sondes de distribution ; et une unité de sortie (45) qui émet un avertissement de distribution anormale lorsque l'unité de détermination (46c) détermine qu'une distribution anormale a lieu.
PCT/JP2011/000721 2010-02-09 2011-02-09 Procédé de gestion de la précision de distribution, procédé de correction d'un volume distribué, procédé de gestion de performances d'agitation, dispositif d'analyse automatique et kit d'analyse Ceased WO2011099279A1 (fr)

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JP2010026705A JP2011163910A (ja) 2010-02-09 2010-02-09 分注精度管理方法、分注量補正方法、攪拌性能管理方法、自動分析装置および分析キット

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JPH09257796A (ja) * 1996-03-19 1997-10-03 Olympus Optical Co Ltd 分析装置および分析方法
JP2000105239A (ja) * 1998-09-29 2000-04-11 Hitachi Ltd 生化学自動分析装置
JP2001091521A (ja) * 1999-09-21 2001-04-06 Olympus Optical Co Ltd 自動分析装置
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JP2003083988A (ja) * 2001-09-13 2003-03-19 Olympus Optical Co Ltd 自動分析装置
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JPH04329362A (ja) * 1991-04-30 1992-11-18 Shimadzu Corp 自動分析装置
JPH09257796A (ja) * 1996-03-19 1997-10-03 Olympus Optical Co Ltd 分析装置および分析方法
JP2000105239A (ja) * 1998-09-29 2000-04-11 Hitachi Ltd 生化学自動分析装置
JP2001091521A (ja) * 1999-09-21 2001-04-06 Olympus Optical Co Ltd 自動分析装置
JP2003004753A (ja) * 2001-06-18 2003-01-08 Aloka Co Ltd 分注良否判定装置
JP2003083988A (ja) * 2001-09-13 2003-03-19 Olympus Optical Co Ltd 自動分析装置
JP2007046979A (ja) * 2005-08-09 2007-02-22 Juki Corp 分注量の自動補正方法及びその機能を有する分注装置
WO2008116093A2 (fr) * 2007-03-20 2008-09-25 Becton, Dickinson And Company Dosages utilisant des particules actives en spectroscopie raman amplifiée en surface (sers)

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EP4632386A1 (fr) * 2024-04-10 2025-10-15 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Analyseur d'échantillons

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