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WO2011099027A1 - Composition et procédé d'élaboration de biofongicide à base de trichoderma viride pour lutter contre les maladies transmises par le sol - Google Patents

Composition et procédé d'élaboration de biofongicide à base de trichoderma viride pour lutter contre les maladies transmises par le sol Download PDF

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Publication number
WO2011099027A1
WO2011099027A1 PCT/IN2010/000185 IN2010000185W WO2011099027A1 WO 2011099027 A1 WO2011099027 A1 WO 2011099027A1 IN 2010000185 W IN2010000185 W IN 2010000185W WO 2011099027 A1 WO2011099027 A1 WO 2011099027A1
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composition
rot
growth
days
culture
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PCT/IN2010/000185
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English (en)
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Chetan S. Patel
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma

Definitions

  • the present invention relates generally to a composition and method of making fungal based product containing Trichoderfna viride for controlling various soil born disease.
  • 0083/CAL/2000 - Granted Patent wherein the invention relates to a process for preparing a novel biocide comprising a consortium of two bacterial and two fungal strains for controlling a severe disease of tea plants caused by pathogenic fungal strains namely Corticium invisum and Corticium theae..
  • the preparation of the biocide is made by mining the four ingredients strains at the ratio of 4:3:2:1 and the culture, are prepared in the following manners a) Transferring the Bacillus subtilis and Pseudomanas putida cultures into mother culture scandium, b) allowing the another cultures to grow for 3— 7 day., c) transferring the grown mother cultures to main culture m4dium and allowing the main cultures to grow for 3— 7 days, d) transferring the Tnichoderma viridi and Trichoderma harzianum cultures in the mother culture medium, e) allowing the mother cultures to grow for 15 days, (f) transferring the mother cultures 4 ⁇ to the main culture, medium and allowing the main cultures to grow for 15 days, g) mixing the said ready cultures— Bacillus subtilis, Pseudomonas putida, Trichoderma viridi and Trichoderma harzianum in a ratio of 4:3:2:1 to obtain the biocide.
  • ATCC 74084 ##STR1## which are squalene synthase inhibitors and thus useful as cholesterol lowering agents. These compounds are also potent antifungal agents. Additionally, they inhibit farnesyl protein transferase and famesylation of the oncogene protein Ras and are thus useful in treating cancer.
  • This invention also relates to a process for obtaining compounds of structural formula (I).
  • the invention discloses an antagonistic Trichoderma fungal strain that targets soil-born fungi from the genus Fusaria and the genus Rhizoctonia.
  • Trichoderma harzianum In the existing system as given in WIPO Patent Application No. PCT/ES 1996/000206 - Granted Patent wherein the invention relates to liquid formulation based on strains of the stringy fungi Trichoderma harzianum and Trichoderma viride.
  • It comprises sources of carbon, mineral salts and traces of elements with organic and - inorganic nutrients, and vegetative structures reproducing strains of the stringy fungus Trichoderma harzianum Rifai which have been identified as Trichoderma harzianum IMI 352940, Trichoderma harzianum IMI 352941 , and Trichoderma harzianum CECT 20179; and Trichoderma viride Pers.: Fr identified as Trichoderma viride CECT 20178.
  • the formulation is useful as a biological control agent for combatting diseases of plants and plant material and biodamage produced or transmitted by stringy fungi and other pests of plants, and as a biolixiviation agent.
  • the invention concerns a mycofungicidal product consisting of immunizing commensals (:IC) and cereal flour where the immunizing commensal is Scytalidium lignicola ATCC 16,675, Trichoderma polysporum Rifai ATCC 20,475, Trichoderma viride sensu Bisby ATCC 20,476 or Trichoderma viride sensu Bisby strain CG BINAB.INRA T 030(Pasteur Institute, Paris). It is aimed also at uses of this product for the treatment of trees, wood and plants.
  • the rhizosphere-competent strains are particularly effective biocontrol agents for plant and/or treeidiseases associated with various fungi e.g., Pythium spp., $i(Sclerotium), spp., $i(Rhizoctonia solani) and basidiomycetes such as $i(Chondrostereum purpureum).
  • the inventor has invented a composition and method of making a fungal based product containing Trichoderma viride for controlling various soil born diseases like root rot, set rot, wilt, collar rot, red rot, rhizome rot, damping off, stem rot and club rot.
  • This invention relates to a composition and method for utilizing fungal based product containing Trichoderma viride for controlling various soil born diseases like root rot, set rot, wilt, collar rot, red rot, rhizon e rot, damping off, stem rot and club rot.
  • Soil borne disease have until recently, received less attention than plant diseases affecting the shoot and foliage. However, this is not a reflection of their economic importance, but rater of the difficulties in investigating and detecting pathogens below soil level. Many soil born diseases are stress related and it is in the tropics where crop growth is particularly limited by environmental stress, predisposing crops to infection by soil borne pathogen.
  • the present invention is an innovative combination of fungal based product containing Trichoderma viride with enzymes, fats and growth promoting molecules to control soil borne diseases like root rot, set rot, wilt, collar rot, red rot, r izome rot, damping off, stem rot, club rot and alike.
  • Trichoderma viride is inoculated aseptically on plate having 20 ml PAC-00 agar Media; such two to three plates are generally inoculated. Such inoculated plate is maintained in BOD incubator for at 26 ⁇ 1 °C for 6 to 7 days.
  • Composition for one liter of PAC-00 Media contains 300 gm potato, 20 gm dextrose, 20 gm agar agar and 1000 ml distilled water
  • Step-2 Starter culture (In flask)
  • Step-3 Seed Fermentor (In small fermentor)
  • One (1) lit culture grown in step-2 is inoculated aseptically in small fermenter having 10 litter PAC-00 media (Composition for one liter of PAC-00 broth Media contains 300 gm potato, 20 gm dextrose and 1000 ml distilled water), and allow it for further growth at 26 ⁇ 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM Step-4 Add to solid substrate (poly bags)
  • 20 ml culture form fermenter is inoculated aseptically in previously sterilized poly propelin polybag (12 x 10 inch) having 180 gm broken sorghum gain. After inoculation of culture mouth opening of poly bag seal with sponge and allowed to develop. Such 500 bag get inoculated from 10 lit broth recovered from fermentor
  • Step-8 Mixing (Liquid carrier, emulsifier)
  • 10 % fungal spore powder is than mixing in mineral oil (ground nut oil) and tween- 80 emulsifier mixture (90 % ground nut oil + 10 % tween-80) or in case of submerge technique homogenize mixture having spore of fungus where again homogenized by adding 10 to 15 % tween-80 which act as a emulsifier.
  • Mix formulation is allow to cure for two to three days in drums and than send for storage or for packing
  • Step-11 Distribution to farmer, retailer, distributor, etc.
  • the final material prepared has to be used in following form: Dose in one Acre: Liquid Form: 50 ml to 100 ml

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Composition et procédé d'élaboration de biofongicide contenant un mélange de Trichoderma viride et d'enzymes, de matières grasses et de molécules de promotion de croissance pour lutter contre les maladies transmises par le sol du type pourridié, pourriture du semenceau, flétrissures, pourriture du collet, pourriture rouge, pourriture du rhizome, fonte des semis, pourriture de la tige et hernie.
PCT/IN2010/000185 2010-02-09 2010-03-24 Composition et procédé d'élaboration de biofongicide à base de trichoderma viride pour lutter contre les maladies transmises par le sol Ceased WO2011099027A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN343MU2010 2010-02-09
IN343/MUM/2010 2010-02-09

Publications (1)

Publication Number Publication Date
WO2011099027A1 true WO2011099027A1 (fr) 2011-08-18

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2010/000185 Ceased WO2011099027A1 (fr) 2010-02-09 2010-03-24 Composition et procédé d'élaboration de biofongicide à base de trichoderma viride pour lutter contre les maladies transmises par le sol

Country Status (1)

Country Link
WO (1) WO2011099027A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4025394A (en) * 1975-05-06 1977-05-24 The University Of Waterloo Fermentation processes using scraped tubular fermentor
US4837155A (en) * 1983-04-29 1989-06-06 Bio-Technology General Corp. Method of growing trichoderma
US5288634A (en) * 1990-08-03 1994-02-22 Cornell Research Foundation, Inc. Method of increasing the percentage of viable dried spores of a fungus
US20040151699A1 (en) * 2002-07-26 2004-08-05 Salinas Mario Reyes Bactericidal, bacteriostatic and fungicidal composition, comprising two or more live species of trichoderma and its preparation procedure, and a composition based on latex for a pruning cicatrizing that includes a latex base and a composition of two or more live species of trichoderma
US20060292124A1 (en) * 2005-06-22 2006-12-28 Department Of Biotechnology Novel process for commercial production of biopesticides
US20090011480A1 (en) * 2007-06-01 2009-01-08 Solazyme, Inc. Use of Cellulosic Materials for Cultivation of Microorganisms

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4025394A (en) * 1975-05-06 1977-05-24 The University Of Waterloo Fermentation processes using scraped tubular fermentor
US4837155A (en) * 1983-04-29 1989-06-06 Bio-Technology General Corp. Method of growing trichoderma
US5288634A (en) * 1990-08-03 1994-02-22 Cornell Research Foundation, Inc. Method of increasing the percentage of viable dried spores of a fungus
US20040151699A1 (en) * 2002-07-26 2004-08-05 Salinas Mario Reyes Bactericidal, bacteriostatic and fungicidal composition, comprising two or more live species of trichoderma and its preparation procedure, and a composition based on latex for a pruning cicatrizing that includes a latex base and a composition of two or more live species of trichoderma
US20060292124A1 (en) * 2005-06-22 2006-12-28 Department Of Biotechnology Novel process for commercial production of biopesticides
US20090011480A1 (en) * 2007-06-01 2009-01-08 Solazyme, Inc. Use of Cellulosic Materials for Cultivation of Microorganisms

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BROWN ET AL.: "'The effect of acid pH on the growth kinetics of Trichoderma viride.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 17, no. 8, 1975, pages 1199 - 1210 *
ROUSSOS ET AL.: "Strategies for large scale inoculum development for solid state fermentation system: Conidiospores of Trichoderma harzianum.", BIOTECHNOLOGY TECHNIQUES, vol. 5, no. 6, 1991, pages 415 - 420 *
ROUSSOS ET AL.: "Zymotis, a large scale solid state fermenter design and evaluation.", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 42, no. 1, 1993, pages 37 - 52 *
TAO ET AL.: "Effects of air pressure amplitude on cellulase productivity by Trichoderma viride SL- 1 in periodic pressure solid state fermenter.", PROCESS BIOCHEMISTRY, vol. 34, no. ISS. 1, 1 January 1999 (1999-01-01), pages 25 - 29 *

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