WO2011065412A1 - Fucoxanthin-enriched algae product and method for producing the same, and algae lipid and method for producing the same - Google Patents

Abstract

Provided is an algae product that has a low inorganic arsenic content, but is lipid-rich and has a particularly high fucoxanthin content, and further provided is a method for producing the algae product. It is possible to obtain the algae product by means of a method for producing a fucoxanthin-enriched algae product including each of the following steps at least twice: a step for treating a specific type of algae with an organic acid or alkali; and a step for separating the algae that has been treated with the organic acid or alkali.

Description

Processed algal product enriched with fucoxanthin and its production method, algal lipid and its production method

The present invention relates to a processed algal product and a method for producing the same, and more particularly to a processed algal product containing a high concentration of fucoxanthin and a method for producing the same. The present invention also relates to a method for producing algal lipids and algal lipids.

Among algae, lipid components contained in seaweed are known to have various functions. For example, fatty acids such as hexadecatetraenoic acid, octadecatetraenoic acid and eicosapentaenoic acid, and carotenoids such as fucoxanthin and β-carotene are included.

With regard to fatty acids such as eicosapentaenoic acid, functionalities such as blood lipid lowering action, blood pressure lowering action, antithrombotic action, anti-inflammatory action, and anticancer action have been reported (fucoxanthin). , Especially fucoxanthin, anti-obesity effect or anti-obesity effect (Non-patent document 2), high apoptosis-inducing ability to cancer cells (Non-patent document 3), DHA synthesis promoting effect (non-patent document) 4) It has been reported to have functionality such as anti-inflammatory action (Non-patent Document 5), anti-cancer action (Non-Patent Document 6), and anti-diabetic action (Non-Patent Document 7).

However, the lipid content in seaweed is low, and it is necessary to consume a large amount of seaweed in order to exhibit functionality. Therefore, it may be used in the form of lipid extracted from seaweed.

Regarding a method for producing algal lipids (seaweed-derived lipids), for example, a method in which salted kumbu is used as a raw material and extracted with an aqueous ethanol solution having a concentration of 55% or more and 70% or less (Patent Document 1), dry pulverized powder A method of extracting wakame as a raw material using acetone (Patent Document 2) and a method of extracting raw seaweed as a raw material using ethanol with a concentration of 80 to 100% (Patent Document 3) are disclosed.

These production methods can improve workability by increasing the viscosity during extraction, decreasing the efficiency of the solid-liquid separation process, and foaming when the solvent is distilled off under reduced pressure due to the effects of polysaccharides and proteins contained in algae. Has a problem that it significantly decreases. In addition, the extraction method using only an organic solvent reduces the extraction efficiency of algal lipids, i.e., compared to the unit weight of algal bodies used as raw materials, algal lipids such as fucoxanthin and highly unsaturated fatty acids. There is a problem that the extraction amount is small and economically inferior. For example, when extracted using only a highly polar solvent such as ethanol (for example, containing a high-concentration aqueous solution of 50% by weight or more), impurities such as polysaccharides, proteins, inorganic arsenic are mixed in algal lipids, and there are many impurities. In order to increase the purity of algal lipids, there is a problem in that subsequent complicated treatment is required. Furthermore, when the impurities are contained in a large amount, there is a problem that when the removal is performed by washing with water or the like, an emulsion is generated in the process due to the influence, and the recovery rate of the lipid component tends to be remarkably reduced.

In these production methods, an organic solvent is used. However, the use of the organic solvent is not always preferable from an economical viewpoint, and suppression of the use is demanded.

On the other hand, a method of removing a water-soluble component by incubating a refined seaweed in an alginate lyase aqueous solution and then solid-liquid separation is disclosed (Patent Document 4). This method is preferable in that no organic solvent is used, but is not economically preferable in that an expensive enzyme is used.

Also, a method for concentrating lipid components in algal bodies of algae such as kombu has been proposed for the purpose of utilizing algal lipids in the form of algal bodies rather than in the form of lipids (Non-patent Document 8). In this method, algae such as dried kombu are treated with 0.1N hydrochloric acid, and then treated with 0.25% sodium carbonate to increase the content of lipid components in the algae. However, the present inventors have confirmed that when algae such as seaweed, akamoku, and kombu are treated with 0.1N hydrochloric acid, fucoxanthin contained is decomposed, and after the treatment with hydrochloric acid, the algae is drunk when treated with alkali. It has been found that it has a problem that it cannot be recovered as an algal body or becomes extremely difficult and a desired processed seaweed product cannot be obtained.

In addition, as described above, the method described in Non-Patent Document 8 cannot obtain algal alga bodies containing lipid components containing concentrated fucoxanthin at a high concentration. Processing is required, and the problems in the above-mentioned method for producing algal lipids still remain unsolved.

In addition, as a method for separating polysaccharides such as fucoidan from seaweed, a method in which seaweed genus seaweed is powdered and extracted with hot water (Patent Document 5), seaweed is contacted with an aqueous alkali metal salt solution, and then removed from water-soluble components. A method (Patent Document 6), a method of adding water from a seaweed flake or powder and then extracting it by wet grinding (Patent Document 7), and a method of extracting Okinawa mozuku with an organic acid (Patent Document 8). Although it has been reported, these focus on polysaccharides, which are water-soluble components separated from seaweed, and do not focus on lipid components contained in algal bodies that are residues.
In recent years, there are concerns about the effects of inorganic arsenic contained in seaweed on the human body.

JP-A-9-173012 Japanese Patent Laid-Open No. 10-158156 JP 2004-75634 A JP 2009-51791 A Japanese Patent Laid-Open No. 11-113529 JP 2002-105102 A JP 2004-49072 A Japanese Patent No. 3408180

"AA, EPA, DHA-Highly unsaturated fatty acid" edited by Hikaru Kayama, Hoshiseisha Koseikaku H. Maeda et al., Biochemical and Biophysical Research Communications, 332 (2005) 392-397. Hosokawa, Bio Industry, 21 (2004) 52-57. T. Tsukui et al., Journal of Agricultural and Food Chemistry, 55 (2007) 5025. K.Shiratori et al., Experimental Eye Research, 81 (2005) 422-428. M. Hosokawa et al., Biochim. Biophys. Acta, 1675 (2004) 113-119. H. Maeda et al., J. Agric. Food Chem., 55 (2007) 7701-7706. Sato et al., Kitamizu trial, 72 (2006) 19-21

The present invention provides an algal processed product having a reduced content of inorganic arsenic and containing a high concentration of lipid components such as highly unsaturated fatty acids and fucoxanthin, particularly fucoxanthin, and a method for producing the same. The purpose is to do.
Another object of the present invention is to provide an algal lipid production method and algal lipid with good working efficiency and high extraction efficiency.

As a result of intensive studies to solve the above-mentioned problems, the present inventors treated specific algae with an organic acid and / or alkali without using an organic solvent, so that they can be easily recovered as algal bodies, In addition to containing high concentrations of lipid components such as polyunsaturated fatty acids, the degradation of fucoxanthin is suppressed, making it possible to obtain processed algal products that contain particularly high concentrations of fucoxanthin. It has been found that the content of inorganic arsenic in algal bodies treated with acids and / or alkalis or processed algal products is reduced.
Further, the present inventors treated specific algae with an organic acid and / or alkali, and then extracted from the separated algae (algae) using an organic solvent, thereby reducing workability and extraction efficiency. It has been found that algal lipids can be extracted economically because there is no decrease and the amount of organic solvent used can be reduced.
From these findings, the present inventors have completed the present invention.

That is, the first aspect of the present invention is a pine tree, cotton moth, kita wahige, ezoyahaz, hijiki, gananomoku, nejimoku, ezonomemok, hiranemomoku, yoremok, fusujimoku, umitranoo, yamatamomoku, cedar moss, peas , A process of treating one or more algae selected from a green alga belonging to the green alga and a red alga belonging to Susabinori, Asakusanori and Ogonori with an organic acid or alkali, and an algae treated with the organic acid or alkali. The present invention relates to a method for producing a processed algal product enriched with fucoxanthin, comprising at least two steps of separation.

In the present invention, the algae belong to one or more algae classes selected from the group consisting of Matsumo, Watamo, Kitahiwage, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononomemok, Fusizimoku, Umitorano, Yatsumatsumok, Sugimoku, Pea and Akamok. It may be.

In the present invention, the organic acid may be one or more selected from citric acid, malic acid, lactic acid, ascorbic acid and propionic acid. The alkali may be one or more selected from sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide.

Moreover, in this invention, the process which makes the powder of the algae which passed through the process processed with the said organic acid or alkali at least twice may be included.

The second of the present invention relates to a processed algal product enriched with fucoxanthin produced by any one of the production methods described above. In the present invention, the content of inorganic arsenic is preferably 2 ppm or less.

The third aspect of the present invention relates to Matsumo, Watamo, Kitahiwage, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononejimok, Hiraneshimok, Yoremoku, Fusujimoku, Umitoranoo, Yatsumatamamok, Sugimoku, Pepperum, Akamoku, Akamoku, Akamoku, Green A process of treating one or more algae selected from a red-breasted algae and a red seaweed belonging to the red alga class with an organic acid or alkali, and an algae treated with the organic acid or alkali. The present invention relates to a method for producing algal lipid, comprising a step of separating at least twice each, and further comprising a step of extracting a lipid component from the separated algae using an organic solvent.

In the present invention, the organic solvent may be one or more selected from ethanol, acetone and hexane.

In the present invention, the organic acid may be one or more selected from citric acid, malic acid, lactic acid, ascorbic acid and propionic acid.

In the present invention, the alkali may be one or more selected from sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide.

In the present invention, the content of inorganic arsenic in the algae separated in the second separation step may be 2 ppm or less.

The fourth aspect of the present invention relates to an algal lipid produced by any one of the aforementioned algal lipid production methods.

The fifth aspect of the present invention relates to a processed algal product enriched with any of the above fucoxanthins or a composition using the algal lipid.

The sixth aspect of the present invention relates to a processed algal product enriched with any of the above fucoxanthins or a food using the algal lipid, and the food may be for the sick or the elderly. .

According to the present invention, it is possible to provide a processed algal product having a reduced content of inorganic arsenic and containing a lipid component such as a highly unsaturated fatty acid and fucoxanthin, particularly fucoxanthin, and a method for producing the same. it can.
In addition, since the composition using the processed algal product of the present invention as a raw material is provided with functionality by fucoxanthin or the like, the functional component can be safely and simply consumed by ingesting the composition. Can be ingested.
According to the present invention, since the working efficiency and extraction efficiency are good, an economically excellent method for producing algal lipids can be provided. Moreover, the algal lipid obtained by the said manufacturing method contains highly unsaturated fatty acid and / or fucoxanthin in high concentration from mixing of an impurity reduced. Moreover, the algal lipid may be one from which inorganic arsenic has been effectively removed.
In addition, since the composition using the algal lipid of the present invention as a raw material is given functionality by a highly unsaturated fatty acid and / or fucoxanthin, a functional component can be obtained by ingesting the composition. It can be ingested safely and conveniently.

Hereinafter, the present invention will be described in more detail.
First, a method for producing a processed algal product enriched with fucoxanthin of the present invention will be described.
The method for producing a processed algal product enriched with fucoxanthin according to the present invention includes Matsumoto, Watamo, Kitahiwage, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononejimok, Hiraneshimok, Yoremoku, Fusujimoku, Umitora no Moe, Yatsumata A step of treating one or two or more algae selected from a red seaweed, a seaweed, a macabu and a gagome kombu, a green alga belonging to the green alga, and a sausobiori belonging to the red algae class, two or more algae selected from an organic acid or an alkali, and Each of the methods includes a step of separating algae treated with an organic acid or an alkali at least twice.

By the production method including such steps, lipid components such as highly unsaturated fatty acids and fucoxanthin, which are easy to recover as algae bodies of algae (for example, seaweed), are suppressed in decomposition of fucoxanthin, and have functionality, In particular, processed algal products (for example, processed seaweed products) containing fucoxanthin at a high concentration can be obtained. Moreover, since an organic solvent is not used, it is suitable from the economical viewpoint, for example, as a method for producing food.

In the present invention, as described above, the step of treating with an organic acid or alkali and the step of separating the algae treated with the organic acid or alkali may be included at least twice, for example, organic acid treatment of algae. May be performed at least twice, or alkali treatment may be performed at least twice. Further, after the organic acid treatment is performed once, the alkali treatment may be performed once, or in the reverse order, or the treatment of these organic acids and alkalis may be further continued several times. You may go. Moreover, it is not limited to these, An organic acid process and an alkali process can be combined suitably. In the above description, the separation step is omitted for convenience.

As described above, the step of treating with the organic acid or alkali treatment and the step of separating the algae treated with the organic acid or alkali may be performed at least twice, but the highly unsaturated fatty acid and fucoxanthin are more efficiently used. From the viewpoint of concentrating and obtaining processed algal products containing them in high concentration, it is preferable to perform treatment with an organic acid and treatment with an alkali.
For example, a step of treating with an organic acid or alkali, a first separation step of separating the algae treated with the organic acid or alkali, a step of treating the algae separated by the first separation step with an alkali or organic acid, the alkali Or the manufacturing method including the 2nd isolation | separation process which isolate | separates the algae processed with the organic acid is mentioned. Hereinafter, the method for producing a processed algal product enriched with fucoxanthin according to the present invention will be described based on the embodiment in which the algae is treated with an organic acid and an alkali as described above. It is not limited to.

In the present invention, in the algal body after processing the algal product or the algal body after treatment with a predetermined organic acid and alkali, a high concentration or high content of highly unsaturated fatty acid or fucoxanthin is not necessarily algal such as processed seaweed product. Processed products or specified organic acids and fucoxanthins of algae such as specific seaweeds as raw materials, not based on the level of fucoxanthin content of algal bodies after treatment with algae It is a concept including evaluating the rate of increase (concentration rate) of the content of processed algae such as processed seaweed obtained by the above method with respect to the content of. This is because the content of fucoxanthin and the like varies depending on the type of algae, season, and habitat. Therefore, in the processed algal product according to the present invention, the content of fucoxanthin per dry weight is the content of fucoxanthin per dry weight of algae such as seaweed in the raw material stage before the organic acid and alkali treatment. When it is concentrated 1.2 times or more, preferably 1.4 times or more, more preferably 1.8 times or more, the amount of fucoxanthin can be said to be high or high. And The dry weight refers to the algae (algae algae) such as seaweed as a raw material and processed algae such as processed seaweeds or algae after a separation process at 40 ° C. for 15 to 24 hours. It shall mean the weight when dried.

Further, the polyunsaturated fatty acid may vary depending on the type of algae, but linolenic acid (18: 3), stearidonic acid (18: 4), arachidonic acid (20: 4), eicosapentaenoic acid (20: And polyunsaturated fatty acids having 3 or more double bonds, polyunsaturated fatty acids having 4 or more double bonds, and the like.

Furthermore, it becomes possible to reduce the content of inorganic arsenic in algae such as seaweed by the action of organic acids.

Algae used in the present invention are Matsumoto, Watamo, Kitahiwage, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononezmok, Hiranekimoku, Yoremoku, Fusujimoku, Umitranoo, Yatsumatamamok, Sugimoku, Akamoku, Akamobu, Amakomo, Akamoku, Akamo It is 1 or 2 or more types of algae selected from the Boaonori which belongs to a class, and Susabinori, Asakusanori and Ogonori which belongs to a red alga class.

Among these algae, one or more algae belonging to one or more brown algae selected from the group consisting of Matsumo, Watamo, Kitahiwage, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononekomok, Fusizimoku, Umitorano, Yatsumatamok, Sugimoku, Peamo and Akamok It is also preferable to use These algae (sea algae) are preferable in terms of the content of lipid components, particularly fucoxanthin, and among them, fucoxanthin content is higher, and from the viewpoint of eating experience and availability, akamoku is more preferable.

In the present invention, the algae (sea algae) described above are used in principle, but any algae (sea algae) may be used in combination with the algae (sea algae) as appropriate.
Specific examples of the algae (seaweed) that can be used in combination include havanori, mosquitoes, ezofuro, Okinawa mozuku, ishimozuk, chigaiso, tsurumo, herayahaz, shiwayahaz, yahagususa, ajigusa, fukurinamiji, tsuba common gusa, sandadagusa, usa Futaemoku, Owanokomokumoku, Isomoku, Yokomokumoku, Togemoku, Tamahakumoku, Narasamo, Tamanashimok, Mametawara, Obamoku, Nagashimamoku, Yoremoku, Usubamoku, Hiranemoku, Yuzumokumoku, Yoremokumoku Can be illustrated.

As the algae such as seaweed used in the present invention, any algae in various states such as raw algae, frozen algae, salted algae, and dried algae can be used. In terms of economy, raw algae are preferable, but algae frozen from the viewpoint of improving the removal efficiency of polysaccharides and the like by preserving the algae, breaking the algal tissue, and improving the permeability of treatment liquids such as acids and alkalis. Alternatively, dried algae are preferred. The algae can be used as they are or in the state of being shredded, but in terms of improving the removal efficiency of polysaccharides and the like and ease of handling, the algae are finely cut to a size of about 0.5 to 50 mm. A disconnected state is preferred.

In one embodiment of the present invention, as described above, a specific algae is treated with an organic acid and an alkali, the algae subjected to these treatments are separated, and a processed algal product enriched with fucoxanthin is produced. After the organic acid treatment, the alkali treatment may be performed, or after the alkali treatment, the desired algal processed product (for example, seaweed processed product containing high fucoxanthin content) ) Can be obtained. Therefore, here, the former case, that is, the case where the alkali treatment is performed after the organic acid treatment is described as an example, and the description of the latter is omitted, but the latter case is also the same as the former case. Needless to say, the same effects can be obtained.

In an example of an embodiment of the present invention, algae such as seaweed in various states as described above are appropriately pretreated such as washing and desalting as necessary, and then the algae are treated with an organic acid. In addition, when using dried algae, you may use what was returned to the wet state with the tap water etc. By performing the organic acid treatment in this way, unlike the case of alkali treatment after using an inorganic acid such as hydrochloric acid or sulfuric acid, it is easy to recover as an algal body, and polysaccharides, proteins, etc. are eluted out of the algal body. At the same time, lipid components such as highly unsaturated fatty acids and fucoxanthin are retained in the algae while the degradation of fucoxanthin is effectively suppressed. Further, inorganic arsenic is effectively eluted from the algal bodies and removed.

The treatment method with an organic acid is not particularly limited, and a known method may be appropriately selected. For example, the algae is immersed in a solution containing an organic acid having a predetermined concentration (for example, an aqueous solution of an organic acid). Examples thereof include a method of standing still, a method of performing immersion, stirring, shaking and the like. At this time, the mixing ratio of the algae and the organic acid solution is not particularly limited, but from the viewpoint of the removal efficiency of polysaccharides and the like per unit time and the economy, it is generally about 100 parts by weight (dry weight) of the algae. The concentration of the organic acid can be appropriately determined depending on the strength of various acids and the like, but is generally about 0.1 to 10% by weight. The treatment temperature is not particularly limited, but is preferably 20 to 80 ° C., and preferably 25 to 60 ° C. from the viewpoint of removal efficiency of polysaccharides and the like, stability of lipid components such as fucoxanthin and economy. More preferred. The treatment time is not particularly limited, but is preferably 5 to 180 minutes and more preferably 5 to 90 minutes from the viewpoint of removal efficiency of polysaccharides and the economy.

The organic acid is not particularly limited as long as it does not decompose fucoxanthin. For example, oxalic acid, citric acid, succinic acid, malonic acid, cinnamic acid, tartaric acid, adipic acid, fumaric acid, Use one or a combination of two or more selected from maleic acid, phthalic acid, malic acid, lactic acid, butyric acid, propionic acid, ascorbic acid, benzoic acid, valeric acid, glutaric acid, glycolic acid and sorbic acid. Can do. In addition, by using an organic acid as in the present invention, it is possible to prevent slime from being generated on the surface of algal bodies after the alkali treatment described below. In addition, citric acid, malic acid, lactic acid, ascorbic acid, and propionic acid are preferable from the viewpoint of preventing decomposition of fucoxanthin and preventing slime generation on the surface of the algal body after alkali treatment.

In an example of one embodiment of the present invention, after performing an organic acid treatment of algae such as seaweed as described above, the algae treated with the organic acid are separated, and polysaccharides are removed to remove lipid components and the like. Can be obtained while maintaining its shape (that is, as an algal body) (first separation step). The separation method at this time is not particularly limited, and known methods such as filtration, centrifugation, and filter press can be used. The separated solid part (algae alga body) is preferably washed with water or the like to remove organic acids, if necessary. The washing method is not particularly limited, and may be carried out using a known method. Even if the agglomerates (algae) obtained as a solid part are loosened, they may be stirred slowly in running water. good.

In an example of an embodiment of the present invention, algae such as seaweed separated in the first separation step are treated with alkali. By performing the alkali treatment in this way, polysaccharides and the like that were not extracted with the organic acid are further extracted, while lipid components such as highly unsaturated fatty acids and fucoxanthin are retained in the algal bodies. Algae bodies of algae such as seaweed containing a lipid component, particularly fucoxanthin in a high concentration, can be obtained. In addition, by performing an alkali treatment with an organic acid treatment instead of an inorganic acid, the algal bodies of algae such as seaweed become muddy and cannot be collected or difficult to collect.

The treatment method with alkali is not particularly limited, and a known method may be selected as appropriate. For example, an algae (algae) such as seaweed in a solution containing an alkali at a predetermined concentration (for example, an aqueous alkali solution). And the like, and a method of stirring, shaking and the like. At this time, the mixing ratio of the algae such as seaweed and the alkaline solution is not particularly limited, but from the viewpoints of removal efficiency of polysaccharides and the like per unit time and economical efficiency, the weight is approximately 100 parts by weight (dry weight). The concentration of the alkali is preferably 3 to 100,000 parts by weight, and the alkali concentration can be appropriately determined depending on the strength of various alkalis, but is generally 0.1 to 10% by weight. The treatment temperature is not particularly limited, but is preferably 20 to 80 ° C., and preferably 25 to 60 ° C. from the viewpoint of removal efficiency of polysaccharides and the like, stability of lipid components such as fucoxanthin and economy. More preferred. The treatment time is not particularly limited, but is preferably 5 to 180 minutes and more preferably 5 to 90 minutes from the viewpoint of removal efficiency of polysaccharides and the economy.

The alkali used in the present invention is not particularly limited, and calcium hydroxide, calcium carbonate, sodium hydroxide, potassium hydroxide, sodium carbonate, tripotassium phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate , Disodium hydrogen phosphate, sodium dihydrogen phosphate, trisodium phosphate, sodium acetate, sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, sodium benzoate, potassium sorbate, sodium propionate, sodium bicarbonate , Sodium ascorbate, calcium hydroxide, calcium carbonate, monopotassium citrate, tripotassium citrate, monosodium citrate, trisodium citrate, monosodium succinate, disodium succinate, potassium hydrogen tartrate, sodium tartrate, milk Combined and sodium malate, one or two or more selected from the like can be used. From the aspect of economical efficiency and removal of polysaccharides, one or more selected from sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide are preferred.

After the treatment with the alkali as described above, the algae such as seaweed treated with the alkali are separated (second separation step), the polysaccharides are removed, and the lipid components are further concentrated. Can be obtained while maintaining its shape (ie, as algae). The separation method at this time is the same as that after the organic acid treatment. The separated solid part (algae alga body) may be washed with water as in the case of the organic acid treatment, if necessary, to remove alkali.

Alkaline treatment as described above, washing with water as necessary to obtain a processed algal product that retains the shape of the algal algae (for example, a processed product of seaweed containing a high content of fucoxanthin), is further dried by a known method. You may do it. The drying method is not particularly limited, and examples thereof include a method of drying by natural force such as sun drying, freeze drying, blast drying, hot air drying, vacuum drying, or drying by microwave irradiation. .

In the present invention, the algae (algae processed product) that have undergone both the process of treating with an organic acid and / or the process of treating with an alkali as described above may be powdered. For example, in the example of the above embodiment, the powdering step is carried out after alkali treatment (after washing with water if necessary. After passing through the second separation step), and then refined by a known method in a wet state and then dried. The treatment may be performed, or after drying, it may be refined by a known method. For example, as a method of powdering algae (processed algae) dried by performing a predetermined treatment, there is no particular limitation as long as algae such as seaweed are refined, a crusher, a grinder , Homogenizers, ultrasonic generators, and the like, and these may be used in combination of two or more. The degree of refinement of the powder varies depending on various applications, but the size of the powder particles is generally 850 μm (20 mesh) or less, or 600 μm (30 mesh) or less.

Processed algae such as processed seaweed obtained as described above (algae algae) contain lipid components such as the above-mentioned highly unsaturated fatty acids and fucoxanthin, particularly fucoxanthin in high concentration, Since the content of inorganic arsenic is extremely low, for example, 2 ppm or less, it can be suitably used as a raw material for various uses, for example, foods, beverages, feeds, pharmaceuticals, etc., and used as a raw material for foods. More preferred. And since the composition which uses the said algal processed product mentioned later as a raw material is provided with functionality by fucoxanthin etc., a functional component is safe and simple by ingesting the said composition. Can be ingested. In addition, when heat treatment is performed in preparing the composition, lipid components, particularly fucoxanthin, may be decomposed by heating. However, as in the processed algal product of the present invention, the algae of seaweed and other algae When it is held, it is possible to suppress decomposition due to heating. Therefore, it is particularly effective to use the processed algal product of the present invention when a composition is prepared by heat processing.

Next, the method for producing algal lipids of the present invention will be described.
The method for producing algal lipids of the present invention includes Matsumo, Watamo, Kitaiwahige, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononejimok, Hiraneshimok, Yoremoku, Fusujimoku, Umitoranoo, Yatsumatamok, Sugimoku, Pepperum A process of treating one or more algae selected from Gagome Kombu, Boaonori belonging to the green alga class, and Susabinori, Asakusanori and Ogonori belonging to the red alga class with an organic acid or alkali, and treated with the organic acid or alkali. Each of the steps of separating the algae at least twice, and further comprising the step of extracting a lipid component from the separated algae using an organic solvent.

After obtaining algae bodies of algae from which polysaccharides and proteins have been removed by a production method including such steps, lipid components are extracted from the algae bodies using an organic solvent, so that only conventional organic solvents are used. Unlike the case of using it, algal lipids can be extracted efficiently without increasing the viscosity during extraction, reducing the efficiency of the solid-liquid separation process, and reducing the workability due to foaming when the solvent is distilled off under reduced pressure. it can. In particular, in the present invention, polysaccharides and proteins are removed from algal algal bodies, and algal algal bodies containing lipid components such as polyunsaturated fatty acids and / or fucoxanthin in advance at high concentrations are used efficiently. Algal lipids can be obtained. In particular, when using specific algae belonging to the class of brown algae, unlike when treating with inorganic acids such as hydrochloric acid, fucoxanthin is not decomposed, and in particular, efficiently obtaining algal lipids containing fucoxanthin. Can do.

In the method for producing algal lipids of the present invention, a high concentration or high content of highly unsaturated fatty acid or fucoxanthin in the algal alga body (also a processed algal product) after treatment with a predetermined organic acid and alkali, It has the same meaning as described in the above-described method for producing a processed algal product. In the method for producing algal lipids of the present invention, the highly unsaturated fatty acid has the same meaning as described in the method for producing algal processed products. Therefore, details thereof are omitted here.

Even in the method for producing algal lipids of the present invention, it is possible to reduce the content of inorganic arsenic in the algae by the action of organic acids, so the alga of the present invention obtained using the algae after the predetermined treatment Lipids can also be reduced in inorganic arsenic content.

Algae used in the method for producing algal lipids according to the present invention includes Matsumoto, Watamo, Kitaiwahige, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononejimok, Hiranekimo, Yoremoku, Fusujimoku, Umitranoo, Yatsumatamok, Sugimokoku It is one or two or more kinds of algae selected from wakame, macacombs and gagome kombu, bouuaonori belonging to the green algae class, and sasabiori, asakusanori and ogonoori belonging to the red algae class.
Among these algae, one or more algae selected from wakame, kombu, hijiki, mozuku, akamoku and laver are preferable in terms of easy availability, abundant food experience, and lipid component content. Akamoku is more preferred because of its high content of highly unsaturated fatty acids and fucoxanthin.

In the method for producing algal lipids according to the present invention, the above alga is used in principle, but any algae may be used in combination with the algae as appropriate.
Specific examples of the algae that can be used in combination include Shiomidomo, Yakuzusa, Herayahaz, Shirayahazu, Ajigusa, Kazunoamiji, Fukurinamiji, Phaeojio, Sanadagusa, Umiuchiwa, Okinawachiwa, Atsuba Commonweed, Commonweed, Japanese Pear, , Foot mozuku, shige, iroro, nebarimo, shiwa no kawa, mozuku, nisemo zuku, ezofukuro, hoso zobukuro, fennel spider, haberodoki, shark whale, hoso-kiwawamo, tsukawa-no-kori, gukomenori, habano-mochi Tobacco Rabbit, Keuru Shigusa, Hosobaka Turtle, Chigaiso, Elderly Turtle, Ainu Turtle, Aowakame, Hirome, Tsurum, Sujime, Kaji , Kurome, Arame, Trollocombu, Gagaracombu, Onicombu, Nagacombu, Gohei Kombu, Hibamata, Ezoi Shige, Nebutomok, Jolomok, Futaemoku, Shidamoku, Isomok, Sawglymoku, Azuma Nemomoku, Togemoku , Trumpet, etc .;

Red-tailed algae belonging to the red algae, Bentenamanori, Somewakemanori, Uppulinori, Katabenifukuronori, Benifukuronori, Fukuroragara, Nagaragara, Fusanori, Hirafusanori, Nisefusanori, Ragara, Benimozite , Pyramid, flounder, prickly pear, prickly pear, common plover, striped prickly grass, maca, onyxa, duckweed, nettle, isodantsu, hibirodo, prunus, prunus, black-necked swordfish Ginkgo biloba, Red-billed Ginkgo biloba, Pine tree, Rice bran, Nikumu-kade, Tambanori, Centipede, Huda , Hiramkade, Kyonoshiro, Tsurutsuru, Tosakamatsu, Hitomitsu, Sumidakade, Ibaranori, Kazunobara, Kagiibaranori, Saidaibara, Hosobanosakamodoki, Kainokawa, Saimi, Ootama-Otsunori, Otsuno-Mari , Giant goose, mizogoonori, birch, frog degussa, sunflower plover, peony, fushitsunagi, kosifushi nagi, masagoshibari, tengumo modi, anadrus, egonori, amikusa, hanegisu, igis Kokemodoki, Yuna, Venetian Ginori, Kurosozo, Mitsodezo, Hanesoso, Magilezozo, Busozo, Kiburiitogusa, Shaw Zhou Ke glue, A Liu Shan saw-Hiba, brush Saki sawtooth Hiba, and the like;

The green alga belongs to the green algae, Hoshihimeonori, Usahaoonori, Susiaoonori, Nagaaosa, Button Aosa, Ribbon Aosa, Anaaoosa, Hosojuzumo, Tamajusmo, Futjuzumo, Ooshiogusa, Watashiogusa, Tsuyanoshigusa, Aomogusa Fusaiwazuta, Takatsukizuta, Takanohazuta, Ichizuta, Marubahachiwa, Etogenomayuhaki, Prickly Pear Cactus, Cactus, Suzukakemo, Nagamil, Mill, Hymirmodoki, Hymir, Chromil, Ezomil, Tamamil, Hemimuo , Isosugina, and the like.

In the method for producing algal lipid according to the present invention, the above-mentioned algae can be used, but the state of these algae is the same as that described in detail in the above-mentioned method for producing a processed algal product. Moreover, the method for producing algal lipid according to the present invention comprises treating the above-mentioned specific algae with an organic acid and / or alkali, separating the algae subjected to these treatments, and using an organic solvent from the separated algae. Extracts lipid components to produce algal lipids. And the algae which extract a lipid component using an organic solvent can be obtained by performing the process similar to the manufacturing method of the processed algal product which the fucoxanthin of this invention mentioned above concentrated. Therefore, the method for producing algal lipids according to the present invention also includes a step of treating specific algae with an organic acid or alkali and a step of separating the algae treated with the organic acid or alkali at least twice. This can be the case where only the organic acid treatment is performed twice or more, the case where only the alkali treatment is performed twice or more, the case where the organic acid treatment and the alkali treatment are performed once or more each. However, as mentioned above, from the viewpoint of concentrating highly unsaturated fatty acids and fucoxanthin more efficiently and obtaining algal alga bodies (also processed algae) containing them in high concentrations, treatment with organic acids It is preferable to perform a treatment with alkali. In addition, when the treatment with an organic acid and the treatment with an alkali are performed, the organic acid treatment is performed, the alkali treatment is performed, or the alkali treatment is performed and then the organic acid treatment is performed to obtain an algal alga body. The desired algal lipid can be obtained by solvent extraction using the algal cells.

Thus, in the method for producing algal lipids according to the present invention, specific algae subjected to the organic acid treatment and / or alkali treatment substantially the same as the above-described method for producing a processed algal product are used. Therefore, although the embodiment of the method for producing algal lipid according to the present invention will be described below, the algae obtained by the production method shown in the example of the embodiment in the method for producing a processed algal product according to the present invention described above. A production method for obtaining a desired algal lipid using a processed product (algae alga body) will be described. Therefore, detailed description of the organic acid treatment, the alkali treatment, the first separation step, and the second separation step is omitted here. In addition, although the process after a 2nd isolation | separation process is demonstrated below, the following description demonstrates the example of one Embodiment in the case of performing an alkali treatment after performing an organic acid process.

In the method for producing algal lipid according to the present invention, as described in detail in the embodiment of the method for producing a processed algal product described above, after the treatment with an alkali, the algae treated with the alkali are separated. (Second separation step), the algae from which the polysaccharides and the like are removed and the lipid components and the like are further concentrated can be obtained while maintaining the shape. The separated solid part (algae) is preferably washed with water as in the case of the organic acid treatment, if necessary, to remove the alkali.

As described above, an algae containing a high concentration of highly unsaturated fatty acid and / or fucoxanthin, which retains the shape of the algal alga body, is obtained by alkali treatment and washing with water as necessary. In addition, the algae (algae) have a reduced content of inorganic arsenic, and the content is preferably 2 ppm or less.

In the present invention, lipid components are extracted from the algae separated by the second separation step (and further washed with water if necessary) using an organic solvent, but separated by the second separation step (and further washed with water if necessary). After that, the lipid component may be extracted from the algae using an organic solvent as it is (containing water), or after being subjected to various processing or being stored in various forms after being separated, Lipid components may be extracted from algae using an organic solvent.

The processing performed before the lipid component extraction is not particularly limited as long as it is a processing suitable for extraction and storage, and known processing can be performed in addition to drying processing, pulverization processing, freezing processing, and the like. Moreover, as a preservation | save aspect, well-known methods can be used other than the normal temperature preservation | save in a dry state, the freezing / refrigeration preservation | save in the state containing a water | moisture content or a dry state, the salt storage in the state containing a water | moisture content, etc.

The drying treatment is not particularly limited, and examples thereof include a method of drying by natural force such as sun-drying, a method such as freeze drying, blast drying, hot air drying, vacuum drying or microwave irradiation. It is done.

As the pulverization treatment, after the second separation step (after washing with water if necessary), the algae bodies of the obtained algae may be refined in a wet state by a known method to form a paste or juice. After miniaturization, it may be dried to form a powder. Further, after the second separation step (after washing with water if necessary), the obtained algal alga body may be dried and then refined by a known method to form a powder. The method for miniaturization is not particularly limited as long as the algae are miniaturized, and can be performed using a pulverizer, an attritor, a homogenizer, an ultrasonic generator, and the like. A combination of the above may also be used. In addition, the degree of refinement when powdered is not particularly limited, but from the viewpoint of efficiently performing extraction with an organic solvent, the size of the powder particles is generally 2 mm (10 mesh) or less. preferable.

Organic solvent from algae separated in the second separation step (including algae subjected to the above various treatments if necessary. In addition, algae subjected to the extraction operation through a predetermined treatment step may be referred to as processed algae). There is no particular limitation on the method for extracting the lipid component using the above-mentioned method, and the method is a method in which the algae is immersed in an organic solvent and left to stand, a method in which the algae is immersed, stirred, and shaken in the same manner. A spraying method or other known methods can be used.

The organic solvent used in the present invention is not particularly limited as long as the lipid component can be extracted from the algae (algae processed product) separated in the second separation step. Alcohols, ethers, ketones, aliphatic carbonization Hydrogen halogen compounds, aliphatic hydrocarbons, aromatic hydrocarbons, and the like can be used, and one or more selected from these can be used. Among these, from the viewpoint of efficiently extracting the lipid component, it is preferable to use one or more selected from methanol, ethanol, acetone, ethyl acetate, methylene chloride, chloroform, and hexane.
Moreover, when using a water-soluble organic solvent, the liquid mixture of an organic solvent and water can also be used, and the said liquid mixture is also contained in an organic solvent. The concentration of the organic solvent at this time is not particularly limited as long as the algal lipid can be extracted efficiently, but the actual concentration of the organic solvent is preferably 30 to 80% by volume.

In the present invention, when the lipid component is extracted from the algae separated in the second separation step using an organic solvent, for example, when the algae is immersed in an organic solvent, the extract and the solid part (algae) are added. To separate. There is no restriction | limiting in particular as a separation method, Well-known methods, such as filtration (suction filtration, a filter press, etc.), centrifugation, can be used.

The algal lipid of the present invention can be obtained by removing the organic solvent from the extract obtained as described above. The method for removing the solvent is not particularly limited as long as it does not adversely affect the algal lipid, and a known method such as a method of distilling off the organic solvent under reduced pressure can be appropriately employed.
At that time, in order to remove impurities contained in a small amount in the extract to obtain a higher purity algal lipid, for example, the algal lipid obtained by once removing the organic solvent from the extract as described above is used. Then, after adding the organic solvent again, filtration is performed, the organic solvent may be similarly removed from the obtained filtrate, and the operation may be repeated a plurality of times. Further, an inert gas such as nitrogen gas may be sprayed on the algal lipid in order to completely remove the solvent and in consideration of oxidation of the algal lipid.

In order to obtain higher purity algal lipids, the extract obtained in the process of removing impurities from the extract or the extract or a solution obtained by adding an organic solvent to the filtrate is placed on a column supporting silica gel or the like. It may be purified by using a method for obtaining a predetermined fraction through other known means.
In addition, what is necessary is just to perform each said highly purified process suitably as needed in consideration of manufacturing cost.

In the present invention, as described above, an organic acid treatment and an alkali treatment are once performed to obtain an algal body (algae processed product) from which polysaccharides, proteins, and the like have been removed in advance, and the alga processed product is used for the algae. Since extraction of lipid is performed, unlike conventional extraction using raw algae such as seaweed, the viscosity during extraction increases, the efficiency of the solid-liquid separation process decreases, and the solvent is kept under reduced pressure. It is possible to effectively suppress a decrease in workability due to foaming at the time of leaving and a decrease in algal lipid extraction efficiency. In addition, even when high-purity treatment to obtain higher-purity algae lipids is performed, impurities such as proteins and polysaccharides have already been removed from the algae lipids. Almost no influence by the above, etc., effectively reducing the recovery rate, and can obtain algal lipids with higher purity efficiently. In addition, the amount of organic solvent used during extraction can be reduced. Thus, the present invention can extract algal lipids that are economically excellent.

The algal lipid obtained as described above contains a high concentration of lipid components such as highly unsaturated fatty acids and / or fucoxanthin, and is previously extracted from algal bodies with extremely reduced inorganic arsenic. Therefore, the content of inorganic arsenic in the algal lipid can be made extremely low. Therefore, the algal lipid of the present invention can be suitably used as a raw material for various uses, for example, foods, beverages, feeds and pharmaceuticals, and more preferably used as a raw material for foods. And since the composition using the algal lipid as a raw material is given functionality by polyunsaturated fatty acid and / or fucoxanthin, etc., by ingesting the composition, the functional component is safe, And it can be ingested easily.

Examples of the composition when the processed algal product such as processed seaweed obtained by the method for producing processed algal product of the present invention is used for foods include seaweed products such as plate-like dried seaweed and boiled fish; bread, butter Breads such as rolls and bagels; confectionery such as baked goods, short breads and cakes; noodles such as udon and soba noodles; cooked rice such as rice, rice balls and rice cakes; Pasta such as spaghetti, fettuccine, penne, erike, ravioli and lasagna; dressings such as mayonnaise, salad creamy dressing, semi-solid dressing, emulsified liquid dressing and separated liquid dressing; diseases such as mousse, jelly and soup Food for the elderly or elderly .

The plate-like dried seaweed is in a form typified by dry laver, and the ingredients are not particularly limited as long as it is in the form of dry laver. If necessary, it may be baked using far infrared rays or the like, or seasoned with salt, mirin, or the like, and seasoned.

The food for the sick or the elderly includes a semi-solid food or a liquid food such as a swallowing meal or a meal for persons with difficulty in chewing.

The form of the food is not particularly limited. For example, in the case of a bar-type food, a block-type food or a cheerback-type food, the oral intake is easy. A bar-type food is a food having a form such as “SOYJOY (registered trademark) (Otsuka Pharmaceutical Co., Ltd.)”. If the form is a bar-type, the form of processed algae such as seaweed processed products is limited. What is necessary is just to use the thing of various states, such as powder form and small piece form.

Compositions when processed algal products such as processed seaweed products are used for beverages, for example, juices such as vegetable juice, orange juice and apple juice; milk beverages such as milk and fermented milk; fruit wine and sake Examples include alcoholic beverages; tea beverages such as green tea, black tea, and herbal tea.

Examples of compositions in which processed algal products such as processed seaweed products are used for feed include, for example, feed for pets, feed for livestock, and feed for seafood. Examples of the feed for pets include feeds for mammals such as dogs, cats, rabbits, hamsters and squirrels; and feeds for birds such as budgerigars, pigeons, bunchos and juvenile pine. Examples of livestock feed include feed for mammals such as cattle, pigs, sheep and goats; and feed for birds such as chickens, chabos, quails, turkeys, ducks, geese and ostriches. Furthermore, examples of the feed for seafood include feed for cultured fish such as red sea bream, yellowtail, eel and shrimp; feed for ornamental fish such as carp, goldfish, discus, arowana and neon tetra.

Examples of the composition when using processed algal products such as seaweed processed products for pharmaceutical applications include anti-obesity agents, blood sugar level increase inhibitors, cancer cell proliferation inhibitors, anti-inflammatory agents, etc. Various forms such as tablets, powders and capsules can be selected as necessary.

Examples of the composition when the algal lipid obtained by the method for producing algal lipids of the present invention is used for foods include breads such as bread, butter roll and bagels; confections such as baked goods, short breads and cakes Pastas such as spaghetti, fettuccine, penne, erike, ravioli and lasagna; dressings such as mayonnaise, salad creamy dressing, semi-solid dressing, emulsified liquid dressing and isolated liquid dressin; for patients such as mousse, jelly and soup Or the food for elderly people etc. can be mentioned. The food for the sick or the elderly includes a semi-solid food or a liquid food such as a swallow meal or a meal for persons with difficulty in chewing.

The form of the food is not particularly limited. For example, in the case of a bar-type food, a block-type food or a cheerback-type food, the oral intake is easy. The bar-type food means a food having a form such as “SOYJOY (registered trademark) (Otsuka Pharmaceutical Co., Ltd.)”.

Examples of the composition when algal lipid is used for feed include pet feed, livestock feed, and fishery feed. Examples of the feed for pets include feeds for mammals such as dogs, cats, rabbits, hamsters and squirrels; and feeds for birds such as budgerigars, pigeons, bunchos and juvenile pine. Examples of livestock feed include feed for mammals such as cattle, pigs, sheep and goats; and feed for birds such as chickens, chabos, quails, turkeys, ducks, geese and ostriches. Furthermore, examples of the feed for seafood include feed for cultured fish such as red sea bream, yellowtail, eel and shrimp; feed for ornamental fish such as carp, goldfish, discus, arowana and neon tetra.

Examples of the composition when algal lipids are used for pharmaceuticals include anti-obesity agents, blood glucose level increase inhibitors, cancer cell proliferation inhibitors, anti-inflammatory agents, etc. Various kinds of powders, capsules and the like can be selected as necessary.

Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to these examples.

First, a method for producing a processed algae product of the present invention, a processed algae product obtained by the manufacturing method (particularly a processed seaweed product), and an example of a food that is a composition using the processed algae product (processed seaweed product) will be described. To do.

(Frozen storage of seaweed)
The harvested seaweeds were frozen at −20 ° C. and stored at −20 ° C. until use.

(Thawing and desalting of frozen seaweed)
Various frozen seaweeds were thawed and desalted with tap water. After thawing and desalting, the water was drained using a 30-mesh (aperture 600 μm) sieve and used for the preparation of a seaweed processed product described below.

(Measurement of total lipid content)
<Processed seaweed products>
Distilled water (50 mL) was added to 5 g of the dried seaweed processed product (dried sample) obtained in Examples 1 to 3 and Comparative Examples 1 to 4, mixed, and allowed to stand in the dark at room temperature for 15 minutes. After standing, 200 mL of chloroform / methanol (1: 2, v / v) was added, mixed, and allowed to stand in the dark at room temperature for 1 hour. After standing, filter paper filtration was performed. To the residue, 150 mL of chloroform / methanol (1: 2, v / v) was added, mixed, and allowed to stand at room temperature in the dark for 1 hour. After standing, filter paper filtration was performed. To the residue, 150 mL of chloroform / methanol (1: 2, v / v) was added, mixed, and allowed to stand at room temperature in the dark for 1 hour. After standing, filter paper filtration was performed. The filtrate obtained by filtering each filter paper was transferred to a separatory funnel, and the lower layer portion was collected. The lower layer was dehydrated by adding anhydrous sodium sulfate to the recovered lower layer. After dehydration, the solvent was distilled off with a rotary evaporator, and nitrogen gas was further blown to obtain a concentrate. The weight of the obtained concentrate was defined as the total lipid weight, and the value obtained by dividing the value by the dry sample weight was defined as the total lipid content.

<Composition>
In the case of Examples 4 to 7, 100 mL of distilled water was added to 50 g of the composition obtained in each Example, and mixed and pulverized with an Oster blender (manufactured by Oster, ST-1 type). After mixing and grinding, the mixture was allowed to stand in the dark at room temperature for 15 minutes. After allowing to stand, 500 mL of chloroform / methanol (1: 2, v / v) was added, mixed, and allowed to stand in the dark at room temperature for 1 hour to obtain a mixture.
On the other hand, in the case of Examples 8 and 9, without adding distilled water to 50 g of the composition obtained in each Example, 500 mL of chloroform / methanol (1: 2, v / v) was added and mixed for 1 hour. The mixture was allowed to stand in the dark at room temperature to obtain a mixture.
Next, suction filtration was performed on each mixture after standing. To the residue was added 300 mL of chloroform / methanol (1: 2, v / v), mixed, and allowed to stand in the dark at room temperature for 1 hour. After standing, suction filtration was performed. To the residue was added 300 mL of chloroform / methanol (1: 2, v / v), mixed, and allowed to stand in the dark at room temperature for 1 hour. After standing, suction filtration was performed. The filtrate obtained by each filtration was moved to the separating funnel, and the lower layer part was collect | recovered. The lower layer was dehydrated by adding anhydrous sodium carbonate sulfate to the recovered lower layer. After dehydration, the solvent was distilled off with a rotary evaporator, and nitrogen gas was further blown to obtain a concentrate. The weight of the resulting concentrate was taken as the total lipid weight.

(Measurement of fucoxanthin content)
Based on the calibration curve, the fucoxanthin content in the total lipid was quantified under the following HPLC conditions, and the fucoxanthin content in the dried seaweed processed product and the composition was calculated from the total lipid content calculated as described above. .
<HPLC conditions>
Pump: L-7100 (manufactured by Hitachi, Ltd.)
Detector: Photodiode array spectrophotometer L-7455 (manufactured by Hitachi, Ltd.)
Column: TOSOH TSKgel ODS-80Ts (250 × 4.6 mm id)
Mobile phase: 30% acetonitrile / methanol Flow rate: 1 mL / min
Column temperature: 28 ° C
Detection wavelength: 450nm

(Quantitative determination of inorganic arsenic)
Inorganic arsenic was quantified by HPLC-inductively coupled plasma mass spectrometry (manufactured by Agilent Technologies, 7500cx). The detection limit was 0.5 ppm.

(Measurement of moisture content (dry weight))
A part of the sample was dried in an air bath at 40 ° C. for 15-20 hours, and the water content was calculated based on the change in weight. The weight after drying was taken as the dry weight of the sample.

Example 1
100 g of akamoku (water content 82.0% by weight) thawed as described above was shredded to a size of about 0.5 to 3 cm and immersed in 400 mL of a 2% by weight aqueous propionic acid solution. The mixture was allowed to stand at 15 ° C. for 15 minutes and treated with an organic acid. After the organic acid treatment, suction filtration was performed, the liquid part was removed, and the solid part (seaweed) was collected. The collected solid part (seaweed) was gently stirred in a 50-mesh (mesh opening 300 μm) sieve and washed with water until the odor of propionic acid disappeared while changing the water. Next, the seaweed after washing with water is immersed in 400 mL of a 2% by weight aqueous sodium carbonate solution (generally, 2950% by weight of a 2% aqueous sodium carbonate solution with respect to 100 parts by weight of seaweed (dry weight)). Then, alkali treatment was performed. After alkali treatment, suction filtration was performed, the liquid part was removed, and the solid part (seaweed) was collected. The collected solid part (seaweed) was gently stirred in a 50-mesh sieve and washed with water until the slime was removed and the odor of sodium carbonate disappeared while changing the water. After washing with water, the obtained seaweed was collected with a 50 mesh sieve to obtain a processed seaweed product. The seaweed processed product was spread on a 50-mesh screen and dried at 40 ° C. for 18 hours to obtain a dried seaweed processed product, that is, an organic acid / alkali-treated dried red moss (treated akamoku-1). Table 1 shows the yield, total lipid content, fucoxanthin content, and inorganic arsenic concentration of the treated akamoku-1 with respect to the dry weight of the raw material akamoku.
The fucoxanthin elution time when the fucoxanthin content was measured by the above-described HPLC method was 3.8 minutes.

(Comparative Example 1)
An untreated dry akamoku (untreated akamoku-1) was obtained in the same manner as in Example 1 except that the 2% by weight aqueous propionic acid solution and the 2% by weight aqueous sodium carbonate solution were each replaced with distilled water. Table 1 shows the yield, total lipid content, fucoxanthin content, and inorganic arsenic concentration of untreated akamoku relative to the dry weight of raw material akamoku. The fucoxanthin elution time when the fucoxanthin content was measured by the above-described HPLC method was 3.8 minutes. In addition, the water washing was performed to the same extent as the water washing after the organic acid treatment and the alkali treatment in Example 1.

(Comparative Example 2)
A 0.1% hydrochloric acid treatment was carried out in the same manner as in Example 1 except that 0.1N hydrochloric acid was used instead of the 2% by weight aqueous propionic acid solution. However, it became a muddy state, and it was not possible to obtain a dried red peach mok treated with hydrochloric acid / alkali. Table 1 shows the fucoxanthin content contained in akamoku after 0.1N hydrochloric acid treatment (hydrochloric acid-treated akamoku). In addition, the fucoxanthin elution time when the fucoxanthin content was measured by the above-described HPLC method was 3.8 minutes, but the peak area was larger than that in the case of treatment with an aqueous propionic acid solution or in the case of no treatment. Diminished.

(Comparative Example 3)
In the same manner as in Example 1, an organic acid treatment with a 2 wt% aqueous propionic acid solution was performed. After washing with water, the obtained seaweed was spread on a 50-mesh screen and dried at 40 ° C. for 18 hours to obtain an organic acid-treated dried red peach (treated red peach mok-2). Table 1 shows the yield, total lipid content, fucoxanthin content, and inorganic arsenic concentration of the treated akamoku-2 with respect to the dry weight of the raw material akamoku. The fucoxanthin elution time when the fucoxanthin content was measured by the above-described HPLC method was 3.8 minutes.

(Example 2)
In the same manner as in Example 1, except that cold-thawed hinoki (water content 83.4%) was used instead of cold-thawed akamoku, and potassium carbonate was used instead of sodium carbonate, organic acid / alkali-treated dried hinoki (Treatment HIKIKI-1) was obtained. Table 1 shows the yield, total lipid content, fucoxanthin content, and inorganic arsenic concentration of treated hinoki-1 relative to the dry weight of raw cypress.

(Comparative Example 4)
In the same manner as in Comparative Example 1 except that cold-thawed cypress was used instead of cold-thawed akamoku, an untreated dried cypress (untreated cypress-1) was obtained. Table 1 shows the yield of untreated hinoki-1, total lipid content, fucoxanthin content, and inorganic arsenic concentration relative to the dry weight of raw hinoki.

(Example 3)
The treated akamoku-1 obtained in Example 1 was pulverized with an oster blender, and a powdery processed akamoku product was obtained through a 100-mesh (mesh size 150 μm) sieve. The total processed lipid content and fucoxanthin content of the powdered processed red peach moku were 111.2 mg / g and 16.4 mg / g, respectively, which were equivalent to the treated akamoku-1 obtained in Example 1.

From Table 1, the content of the total lipid and fucoxanthin per seaweed dry weight of the processed seaweed product (treated akamoku-1) in Example 1 was compared with that of untreated seaweed (untreated akamoku-1) in Comparative Example 1. It is about 1.8 times, and further, it is 1.5 times or more as compared with the seaweed (treated akamok-2) subjected to only the organic acid treatment in Comparative Example 3. Further, comparing Comparative Example 1 and Comparative Example 3, it can be seen that when only the organic acid treatment is performed, the concentration of fucoxanthin or the like is almost the same or slightly higher.
Thus, the organic acid treatment alone is slightly improved in the total lipid content and fucoxanthin content as compared with the untreated case, but by further subjecting the seaweed treated with the organic acid to an alkali treatment. It can be seen that the lipid component containing fucoxanthin remains at a high concentration. In addition, as shown in Table 1, it can also be seen that the concentration of inorganic arsenic is greatly reduced.
Further, as in Comparative Example 3 (same as in Example 1 after the organic acid treatment), the content of fucoxanthin contained in akamok in the treatment with 2% by weight of propionic acid is the same as in Comparative Example 1. The content of fucoxanthin contained in the untreated scallop is almost the same, and there is no decrease due to degradation. On the other hand, as in Comparative Example 2, the content of fucoxanthin in red mock treated with 0.1N hydrochloric acid was 26% of that of untreated red mushroom in Comparative Example 1, and 74% of fucoxanthin was decomposed. It became clear. This point can be confirmed from the HPLC measurement result.

As described above, according to the present invention, even if a specific seaweed belonging to the class of brown algae is subjected to an organic acid treatment and an alkali treatment, a seaweed alga body can be easily recovered and a highly unsaturated fatty acid having functionality. It is possible to obtain a processed seaweed product containing a high concentration of lipid components, such as fucoxanthin and fucoxanthin, and the content of inorganic arsenic is extremely reduced. Further, in Example 1 above, the content of highly unsaturated fatty acid having 3 or more double bonds is 50.3% by weight based on the fatty acid contained in the total lipid, and highly unsaturated fatty acid having 4 or more double bonds. It was confirmed that 43.7% by weight of saturated fatty acid was contained and the other examples were equivalent.
Therefore, if the seaweed processed product of the present invention is used, it is possible to safely and easily ingest a lipid component containing a highly unsaturated fatty acid or fucoxanthin having functionality.

Next, a preferred example of a composition using the processed fucoxanthin-rich seaweed product that is an example of the processed algal product of the present invention will be described.

Example 4
<Preparation of processed seaweed products>
In the same manner as in Example 1, a seaweed processed product (red processed product) before drying was obtained.
<Preparation of composition using seaweed processed product>
Next, 100 g of freshly washed raw laver (produced by Akashi) was added to 100 g of the processed akamoku product (water content 84.2% by weight), and chopped with an oster blender. After shredding, water was added and mixed well, and the mixture was sprinkled over a framed slat. The glue remaining on the slat was dried at 40 ° C. for 20 hours to obtain 32.6 g of plate-like dried seaweed (composition). . The obtained plate-like dried seaweed had a paste-like flavor and had good compatibility with rice, and could be eaten in the same manner as a paste such as rice balls and paste. Moreover, the fucoxanthin content contained in the obtained composition was measured. The results are shown in Table 2.

(Example 5)
<Preparation of processed powdered seaweed>
The processed red crab product obtained in the same manner as in Example 4 was spread on a 50-mesh screen and dried at 40 ° C. for 18 hours to obtain a dried red crab product. The dried akamoku processed product was pulverized with an oster blender and passed through a 140 mesh (mesh opening 106 μm) sieve to obtain a powdered akamoku processed product.
<Preparation of composition using seaweed processed product>
Wash 420g of glutinous rice until the water is clean in a pan case, pour water for 30 minutes, and 300mL of tap water, and make a koji using an automatic home bakery (manufactured by Panasonic Corporation; SD-BM152). did. When starting to boil, 20 g of the above processed powdered red mock-up product and 30 g of mugwort leaves that had been boiled and extracted with cold water and finely chopped and squeezed with water were added. The obtained grass was 680 g. The grass basket was taken out from the bread case to obtain 20 rolled grass baskets. The obtained Kusanagi was able to eat deliciously. Moreover, the fucoxanthin content contained in the obtained composition was measured. The results are shown in Table 2.

(Example 6)
200 g of strong powder, 200 g of durum semolina powder and 50 g of the processed powdered red peach cake obtained in the same manner as in Example 5 were mixed and placed in a bread case. 100 g of whole egg was added to 170 mL of tap water in which 7 g of salt was dissolved, and then 4 g of olive oil was added to prepare a dough using an automatic home bakery. The obtained dough was wrapped in a wrap and aged for 60 minutes in a refrigerator. After aging, the flour was sprinkled on the surface and stretched to a thickness of about 1 mm with a roll. Next, folding was performed in a staircase pattern while shifting the folds, and the width was cut to 5 mm to obtain 640 g of pasta (composition). The obtained pasta had good physical properties and could be eaten deliciously. Moreover, the fucoxanthin content contained in the obtained composition was measured. The results are shown in Table 2.

(Example 7)
In an enamel pan, 200 g of milk and 35 g of sugar were added, and the mixture was heated while mixing until the sugar was dissolved. The fire was stopped, and 10 g of gelatin previously soaked in 40 mL of tap water was added and dissolved. Next, a solution obtained by dispersing 4 g of matcha tea and 6 g of the processed powdered red peach cake in 60 mL of 80 ° C. tap water was added and mixed well. Cool the bottom with ice water and mix the dough while mixing. Then mix meringue prepared with fresh cream 150g, egg white 20g and sugar 5g for 6 or 7 minutes with a whisk. It cooled for hours and obtained 448g of mousses (composition). The resulting mousse was delicious. Moreover, the fucoxanthin content contained in the obtained composition was measured. The results are shown in Table 2.

(Example 8)
<Preparation of processed powdered seaweed>
The processed red crab product obtained in the same manner as in Example 4 was spread on a 50-mesh screen and dried at 40 ° C. for 18 hours to obtain a dried red crab product. The dried akamoku processed product was pulverized by an airflow type pulverizer and passed through a 270 mesh (aperture 53 μm) sieve to obtain a powdered akamoku processed product.
<Preparation of composition using seaweed processed product>
Pre-emulsified 20g corn salad oil, 20g casein sodium, 145g dextrin, 6g indigestible dextrin, 4g powdered processed red mockup, 2g vitamin mix, 3g glycerin fatty acid ester, 4g gellan gum and 796g distilled water, and then emulsified with an emulsifier. Thus, 924 g of the emulsion (composition) was obtained as a liquid food which is a food for the sick or elderly. Moreover, the fucoxanthin content contained in the obtained composition was measured. The results are shown in Table 2.

Example 9
<Preparation of paste-like seaweed processed product>
After the processed red peach cake was prepared in the same manner as in Example 4, the processed red peach cake was obtained by using a mascolloider (manufactured by Masuko Sangyo Co., Ltd.).
<Preparation of composition using seaweed processed product>
10 g of the paste-like red mock processed product, 2 g of matcha tea (commercial product) and 4 g of sugar were added to 200 g of milk and stirred to obtain 215 g of a milk beverage (composition). Moreover, the fucoxanthin content contained in the obtained composition was measured. The results are shown in Table 2.

Next, an example of the algal lipid production method of the present invention, the algal lipid obtained by the production method, and a food product that is a composition using the algal lipid will be described.

(Frozen storage of algae)
The various harvested algae were frozen at −20 ° C. and stored at −20 ° C. until use.
(Thawing and desalting of frozen algae)
Various frozen algae were thawed and desalted with tap water. After thawing and desalting, the water was drained using a 30-mesh sieve (600 μm openings) and used for preparation of various processed algal products.

(Quantitative determination of inorganic arsenic)
Inorganic arsenic was quantified by HPLC-inductively coupled plasma mass spectrometry (manufactured by Agilent Technologies, 7500cx). The detection limit was 0.5 ppm.

(Preparation of processed algae)
100 g of the various algae were chopped to a size of about 0.5 to 3 cm, immersed in 400 ml of a 2% by weight aqueous propionic acid solution, and allowed to stand at 30 ° C. for 15 minutes for organic acid treatment. After the organic acid treatment, suction filtration was performed, the liquid part was removed, and the solid part (algae) was collected. The collected solid part (algae) was gently stirred in a 50 mesh (mesh opening 300 μm) sieve and washed with water until the smell of propionic acid disappeared while changing the water. Next, the algae after washing were immersed in 400 ml of a 2 wt% aqueous sodium carbonate solution and allowed to stand at 30 ° C. for 15 minutes for alkali treatment. After alkali treatment, suction filtration was performed, the liquid part was removed, and the solid part (algae) was collected. The collected solid part (algae) was gently stirred in a 50-mesh sieve, and washed with water until the slime was removed and the odor of sodium carbonate disappeared while changing the water. After washing with water, the obtained algae were collected with a 50-mesh sieve to collect the algae, and a processed algae product was obtained.

(Preparation of processed dried algae)
The processed algal product obtained as described above was spread on a 50-mesh screen and dried at 40 ° C. for 20-24 hours to obtain a dried algal processed product.

(Measurement of water content)
A part of the sample was dried in an air bath at 40 ° C. for 15-20 hours, and the water content was calculated based on the change in weight. The weight after drying was taken as the dry weight of the sample.

(Measurement of total lipid content of composition)
To 50 g of the composition obtained in Example 13 described later, 500 mL of chloroform / methanol (1: 2, v / v) was added, mixed, and allowed to stand in the dark at room temperature for 1 hour to obtain a mixture.
On the other hand, 100 mL of distilled water was added to 50 g of the composition obtained in Example 14, which will be described later, and mixed and pulverized with an oster blender (manufactured by oster, ST-1 form). After mixing and grinding, the mixture was allowed to stand in the dark at room temperature for 15 minutes. After allowing to stand, 500 mL of chloroform / methanol (1: 2, v / v) was added, mixed, and allowed to stand in the dark at room temperature for 1 hour to obtain a mixture.
Next, suction filtration was performed on each mixture after standing. To the residue was added 300 mL of chloroform / methanol (1: 2, v / v), mixed, and allowed to stand in the dark at room temperature for 1 hour. After standing, suction filtration was performed. To the residue was added 300 mL of chloroform / methanol (1: 2, v / v), mixed, and allowed to stand in the dark at room temperature for 1 hour. After standing, suction filtration was performed. The filtrate obtained by each filtration was moved to the separating funnel, and the lower layer part was collect | recovered. The lower layer was dehydrated by adding anhydrous sodium carbonate sulfate to the recovered lower layer. After dehydration, the solvent was distilled off with a rotary evaporator, and nitrogen gas was further blown to obtain a concentrate. The weight of the resulting concentrate was taken as the total lipid weight.

(Measurement of fucoxanthin content)
Based on the calibration curve, the fucoxanthin content in the total lipid was quantified under the following HPLC conditions, and the fucoxanthin content in the dried alga was calculated from the total lipid content.
HPLC conditions Pump: L-7100 (manufactured by Hitachi, Ltd.)
Detector: Photodiode array spectrophotometer L-7455 (manufactured by Hitachi, Ltd.)
Column: TOSOH TSKgel ODS-80Ts (250 × 4.6 mm id)
Mobile phase: 30% acetonitrile / methanol Flow rate: 1 mL / min
Column temperature: 28 ° C
Detection wavelength: 450nm

(Example 10)
Akamoku was used as the algae, and a processed red algae product was obtained according to the preparation of the algae processed product. The inorganic arsenic concentration of the processed red scented product was below the detection limit (0.5 ppm).
Shredded 57.5 g of the processed red peach mushroom (water content 85.4%) to a size of about 0.5 to 3 cm, add 100 ml of ethanol and 50.9 ml of distilled water, add to room temperature and dark for 2 hours. Left to stand. After standing, suction filtration was performed. The solvent of the obtained filtrate was distilled off with a rotary evaporator to obtain a concentrate. To the obtained concentrate, 50 ml of 70% ethanol was added and sonicated to dissolve. Filter paper filtration was performed, and the solvent of the obtained filtrate was distilled off with a rotary evaporator to obtain a concentrate. To the obtained concentrate, 30 ml of 70% ethanol was added and dissolved by sonication. Filter paper filtration was performed, and the solvent of the obtained filtrate was distilled off with a rotary evaporator. Further, nitrogen gas was blown to obtain a concentrate. The obtained concentrate was used as a red cocom lipid. Table 3 shows the amount of red mock lipid and the amount of fucoxanthin obtained.

(Comparative Example 5)
50 g of akamoku (water content 83.2%, referred to as untreated akamoku) used for the preparation of the processed akamoku product of Example 10 was cut into a size of about 0.5 to 3 cm, 100 ml of ethanol and 58. 1 ml was mixed and left at room temperature in a dark place for 2 hours. After standing, a concentrate was obtained in the same manner as in Example 10. The obtained concentrate was used as a red cocom lipid. Table 3 shows the weights of the obtained red mock lipid and fucoxanthin. When the solvent was distilled off by the rotary evaporator, the foaming was remarkably reduced, the degree of vacuum could not be increased, and it took a long time to distill off the solvent as compared with Example 10. It was thought to be due to the influence of polysaccharides and / or proteins present.

(Example 11)
Using hinoki as algae, a hinoki processed product was obtained according to the preparation of the algae processed product. The inorganic arsenic concentration of the processed hijiki was below the detection limit (0.5 ppm).
Cut 57.1 g of the processed hinoki product (water content 86.0%) to a size of about 0.5 to 3 cm, add 100 ml of ethanol and 50.9 ml of distilled water, add to room temperature and dark for 2 hours. Left to stand. After standing, a concentrate was obtained in the same manner as in Example 10. The obtained concentrate was used as hijiki lipid. Table 3 shows the weight of the obtained hinoki lipid and the weight of fucoxanthin.

(Comparative Example 6)
50 g of hinoki (water content 84.0%, referred to as untreated cypress) used for the preparation of the processed hinoki product of Example 11 was cut into a size of about 0.5 to 3 cm, 100 ml of ethanol and 58. 0 ml was mixed and added, and allowed to stand in the dark at room temperature for 2 hours. After standing, a concentrate was obtained in the same manner as in Example 10. The obtained concentrate was used as hijiki lipid. Table 3 shows the weight of the obtained hinoki lipid and the weight of fucoxanthin. Compared with Example 11, it took a long time to distill off the solvent as in Comparative Example 5.

(Example 12)
Akamoku was used as the algae, and after the processed processed algae was obtained according to the preparation of the processed algae, the processed processed dried akamoku was obtained according to the adjustment of the processed processed algae.
Acetone (100 ml) was added to 5 g of the dried red peach processed product, and the mixture was stirred at room temperature in the dark for 2 hours. After stirring, suction filtration was performed. The solvent of the obtained filtrate was distilled off with a rotary evaporator, and nitrogen gas was further blown to obtain a concentrate. The obtained concentrate was used as a red cocom lipid. Table 3 shows the weights of the obtained red mock lipid and fucoxanthin.

(Comparative Example 7)
Instead of the dried processed red peach cake, 5 g of a dried product (referred to as dried untreated akamoku) obtained by drying the dried red peach cake (referred to as untreated akamoku) at 40 ° C. for 15-20 hours. Akamoc lipid was obtained in the same manner as in Example 12 except that it was used. The obtained lipid weight and fucoxanthin content are shown in Table 3.

As is apparent from Table 3, the method for producing algal lipids according to the present invention is the weight obtained by removing the moisture of the processed algal product from each weight shown in Table 3 (dry weight; for example, 8. in Example 10 and Comparative Example 5). 4 g, 8.0 g in Example 11 and Comparative Example 6) It can be seen that the weight of fucoxanthin and algal lipid per unit algae obtained by dividing by 4) is high, and the extraction efficiency is good. Furthermore, unlike the conventional production method, there is no increase in viscosity at the time of extraction, reduction in efficiency of the solid-liquid separation process, foaming when the solvent is distilled off under reduced pressure, and workability and efficiency are good. In Comparative Example 7, the reason why the obtained algal lipid weight and fucoxanthin weight were low was that the polysaccharides and proteins present in the algal bodies were coagulated during drying, so that the permeability of the solvent was hindered. it was thought.

(Example 13)
An emulsifier was used in the same manner as in Example 10 to obtain 2 g of akamoku lipid, 18 g of corn oil, 20 g of sodium caseinate, 145 g of dextrin, 10 g of indigestible dextrin, 2 g of vitamin mix, 3 g of glycerin fatty acid ester, 4 g of gellan gum and 796 g of distilled water. And emulsified to obtain a nutritionally adjusted liquid food. The fucoxanthin content contained in the obtained nutritionally adjusted liquid food was 25.8 mg / 100 g of the nutritionally adjusted liquid food.

(Example 14)
In the same manner as in Example 10, 5 g of red moku lipid, 15 g of corn oil, 250 g of hardened coconut oil, 30 g of hardened palm oil, and 50 g of hardened palm oil were added to 1.5 g of lecithin, glycerin fatty acid ester. 1.5g was added and melt | dissolved at 65 degreeC, and the oil phase part was produced.
On the other hand, an aqueous phase part was prepared by dissolving 60 g of buttermilk powder, 2 g of whey mineral, 5 g of crystalline cellulose, 2 g of vitamin mix, and 1.5 g of sucrose fatty acid ester in 577.5 g of water at 60 ° C. The oil phase part and the aqueous phase part were emulsified with an emulsifier to obtain an akamoku lipid-containing emulsion.
Next, 200 g of milk and 35 g of sugar were placed in an enamel pan. The mixture was heated while mixing until the sugar was dissolved. The fire was stopped, and 10 g of gelatin previously soaked in 40 ml of tap water was added and dissolved. Next, a product in which 10 g of matcha was dispersed in 60 ml of 80 ° C. tap water was added and mixed well. Cool the bottom with ice water and mix the dough with the mixture. Mix the meringue prepared with 150g of whipped cream, 20g of egg white and 5g of sugar, and mix with a whisk. And then cooled in a refrigerator for 3 hours to obtain a mousse. The resulting mousse was delicious.
The fucoxanthin content contained in the obtained mousse was 21.1 mg / 100 g mousse.

The present invention relates to a processed algal product containing a high concentration of lipid components such as highly unsaturated fatty acids and fucoxanthin, particularly fucoxanthin, and a method for producing the same, and by adding the processed algal product to foods, etc. Functionality can be imparted to the food, and the functional component can be ingested safely and conveniently.

The present invention relates to a method for producing algal lipids and algal lipids. Since the working efficiency and extraction efficiency are good, an economically superior method for producing algal lipids can be provided. Moreover, the algal lipid obtained by the said manufacturing method has impurities, such as polysaccharide, reduced more than the conventional manufacturing method, and contains highly unsaturated fatty acid and / or fucoxanthin in high concentration. Moreover, the algal lipid may be one from which inorganic arsenic has been effectively removed. Therefore, by adding the algal lipid of the present invention to foods and the like, it becomes possible to impart functionality to foods, and functional components can be taken safely and simply.

Claims (16)

Matsumoto, Watamo, Kitaiwahiz, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononejimok, Hiranekimo, Yoremoku, Fusujimoku, Umitorano, Yatsumatamamok, Sugimoku, Peamo, Akamoku, Bamboo At least two steps of treating one or more algae selected from Susabinori, Asakusanori and Ogonori belonging to the alga class with an organic acid or alkali, and separating the algae treated with the organic acid or alkali, respectively A method for producing a processed algal product enriched with fucoxanthin, characterized by comprising The algae is an algae belonging to one or more types of brown algae selected from Matsumo, Watamo, Kitaiwage, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononekomok, Fusisujimok, Umitorano, Yasumatsumok, Sugimoku, Peamo and Akamok. A method for producing a processed algal product in which the fucoxanthin according to 1 is concentrated. The method for producing a processed algal product enriched with fucoxanthin according to claim 1 or 2, wherein the organic acid is one or more selected from citric acid, malic acid, lactic acid, ascorbic acid and propionic acid. The process for producing a processed algal product enriched with fucoxanthin according to any one of claims 1 to 3, wherein the alkali is one or more selected from sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide. . The method for producing a processed algal product enriched with fucoxanthin according to any one of claims 1 to 4, comprising a step of powdering algae that has undergone the treatment with the organic acid or alkali at least twice. A processed algal product enriched with fucoxanthin produced by the production method according to any one of claims 1 to 5. The processed algal product enriched with fucoxanthin according to claim 6, wherein the content of inorganic arsenic is 2 ppm or less. Matsumoto, Watamo, Kitaiwahiz, Ezoyahaz, Hijiki, Uganomok, Nejimok, Ezononejimok, Hiranekimo, Yoremoku, Fusujimoku, Umitorano, Yatsumatamamok, Sugimoku, Peamo, Akamoku, Bamboo At least a step of treating one or more algae selected from Susabiori, Asakusanori and Ogonori belonging to algae with an organic acid or alkali, and a step of separating the algae treated with the organic acid or alkali, respectively A method for producing algal lipid, comprising a step of extracting a lipid component from an isolated algae using an organic solvent. The method for producing algal lipid according to claim 8, wherein the organic solvent is one or more selected from ethanol, acetone and hexane. The method for producing algal lipids according to claim 8 or 9, wherein the organic acid is one or more selected from citric acid, malic acid, lactic acid, ascorbic acid and propionic acid. 11. The method for producing algal lipid according to claim 8, wherein the alkali is one or more selected from sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide. 12. The method for producing algal lipid according to claim 8, wherein the content of inorganic arsenic in the algae separated in the second separation step is 2 ppm or less. Algal lipid produced by the production method according to any one of claims 8 to 12. A composition comprising the processed algal product enriched with fucoxanthin according to claim 6 or 7, or the algal lipid according to claim 13. 14. A processed algal product enriched with fucoxanthin according to claim 6 or 7, or a food product using the algal lipid according to claim 13. The food according to claim 15, wherein the food is for a sick person or an elderly person.