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WO2011047265A4 - Analyse par transfert d'énergie de résonance utilisant, comme substrat, une fraction de synaptobrévine - Google Patents

Analyse par transfert d'énergie de résonance utilisant, comme substrat, une fraction de synaptobrévine Download PDF

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Publication number
WO2011047265A4
WO2011047265A4 PCT/US2010/052847 US2010052847W WO2011047265A4 WO 2011047265 A4 WO2011047265 A4 WO 2011047265A4 US 2010052847 W US2010052847 W US 2010052847W WO 2011047265 A4 WO2011047265 A4 WO 2011047265A4
Authority
WO
WIPO (PCT)
Prior art keywords
cell
bont
hybrid
nucleic acid
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/052847
Other languages
English (en)
Other versions
WO2011047265A1 (fr
Inventor
Ward Tucker
Füsûn N. ZEYTIN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BIOSENTINEL Inc
Original Assignee
BIOSENTINEL Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US13/502,357 priority Critical patent/US9453254B2/en
Priority to ES10824166.2T priority patent/ES2576747T3/es
Priority to EP10824166.2A priority patent/EP2488868B1/fr
Priority to DK10824166.2T priority patent/DK2488868T3/en
Application filed by BIOSENTINEL Inc filed Critical BIOSENTINEL Inc
Publication of WO2011047265A1 publication Critical patent/WO2011047265A1/fr
Publication of WO2011047265A4 publication Critical patent/WO2011047265A4/fr
Anticipated expiration legal-status Critical
Priority to US14/941,452 priority patent/US10246492B2/en
Priority to US16/267,155 priority patent/US10508135B2/en
Priority to US16/712,462 priority patent/US10975125B2/en
Priority to US17/208,649 priority patent/US11661442B2/en
Priority to US18/137,604 priority patent/US12054516B2/en
Priority to US18/747,818 priority patent/US12344634B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des compositions et des méthodes pour analyser l'activité protéase intracellulaire de BoNT, en particulier l'activité protéase de BoNT/B, BoNT/G, BoNT/D et/ou BoNT/F. Idéalement, des cellules expriment une ou plusieurs protéines hybrides recombinantes contenant une ou plusieurs protéines fluorescentes et au moins une séquence de reconnaissance et de clivage de protéase de BoNT, l'analyse étant effectuée au moyen d'une analyse FRET (transfert d'énergie de fluorescence par résonance).
PCT/US2010/052847 2009-10-16 2010-10-15 Analyse par transfert d'énergie de résonance utilisant, comme substrat, une fraction de synaptobrévine Ceased WO2011047265A1 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
US13/502,357 US9453254B2 (en) 2009-10-16 2010-10-15 Resonance energy transfer assay with synaptobrevin substrate moiety
ES10824166.2T ES2576747T3 (es) 2009-10-16 2010-10-15 Ensayo de transferencia de energía de resonancia con un resto de sustrato de sinaptobrevina
EP10824166.2A EP2488868B1 (fr) 2009-10-16 2010-10-15 Analyse par transfert d'énergie de résonance utilisant, comme substrat, une fraction de synaptobrévine
DK10824166.2T DK2488868T3 (en) 2009-10-16 2010-10-15 Resonansenergioverføringsassay with synaptobrevinsubstratdel
US14/941,452 US10246492B2 (en) 2009-10-16 2015-11-13 Botulinum assay with synaptobrevin substrate moiety
US16/267,155 US10508135B2 (en) 2009-10-16 2019-02-04 Non-FRET cell based assay
US16/712,462 US10975125B2 (en) 2009-10-16 2019-12-12 Reporting constructs for characterization of Botulinum neurotoxins
US17/208,649 US11661442B2 (en) 2009-10-16 2021-03-22 Vesicles for characterization of botulinum neurotoxins
US18/137,604 US12054516B2 (en) 2009-10-16 2023-04-21 Cells for characterization of botulinum neurotoxins
US18/747,818 US12344634B2 (en) 2009-10-16 2024-06-19 Peptides for characterization of botulinum neurotoxins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25231509P 2009-10-16 2009-10-16
US61/252,315 2009-10-16

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/502,357 A-371-Of-International US9453254B2 (en) 2009-10-16 2010-10-15 Resonance energy transfer assay with synaptobrevin substrate moiety
US14/941,452 Continuation-In-Part US10246492B2 (en) 2009-10-16 2015-11-13 Botulinum assay with synaptobrevin substrate moiety

Publications (2)

Publication Number Publication Date
WO2011047265A1 WO2011047265A1 (fr) 2011-04-21
WO2011047265A4 true WO2011047265A4 (fr) 2011-06-16

Family

ID=43876579

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/052847 Ceased WO2011047265A1 (fr) 2009-10-16 2010-10-15 Analyse par transfert d'énergie de résonance utilisant, comme substrat, une fraction de synaptobrévine

Country Status (7)

Country Link
US (1) US9453254B2 (fr)
EP (2) EP3067696B1 (fr)
DK (1) DK2488868T3 (fr)
ES (2) ES2676277T3 (fr)
LT (1) LT3067696T (fr)
PL (1) PL2488868T3 (fr)
WO (1) WO2011047265A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101932936B (zh) 2007-09-14 2016-04-27 拜奥麦迪逊公司 利用切割序列和间隔子的共振能量转移测定
US10246492B2 (en) * 2009-10-16 2019-04-02 Biomadison, Inc. Botulinum assay with synaptobrevin substrate moiety
US9249449B2 (en) * 2010-01-22 2016-02-02 Japan Science And Technology Agency Probe reagent for measurement of proteolytic activity
JP6156954B2 (ja) 2012-11-21 2017-07-05 イプセン バイオイノベーション リミテッド タンパク分解性にプロセシングされたポリペプチドの製造方法
CA2913686C (fr) * 2013-06-28 2021-08-03 Merz Pharma Gmbh & Co. Kgaa Moyens et procedes pour determiner l'activite biologique de polypeptides de neurotoxine dans des cellules
US20150118701A1 (en) * 2013-10-25 2015-04-30 Biomadison, Inc. Enzyme assay with duplicate fluorophores
CN107109389A (zh) * 2014-08-12 2017-08-29 拜奥麦迪逊公司 具有修饰的轻链特异性的肉毒杆菌神经毒素及其生产方法
CA3024297A1 (fr) 2016-05-16 2017-11-23 Biomadison, Inc. Essai ameliore avec un fragment a base de synaptobrevine
US12019066B2 (en) * 2016-05-16 2024-06-25 Biomadison, Inc. Assay with synaptobrevin based moiety
GB201702500D0 (en) 2017-02-16 2017-04-05 Univ Sheffield Stable vamp reporter assay

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0981633A2 (fr) 1997-02-13 2000-03-01 Memorial Sloan-Kettering Cancer Center Molecules hybrides pour la detection optique des modifications des micro-environnements cellulaires
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US8137924B2 (en) 2003-12-19 2012-03-20 Wisconsin Alumni Research Foundation Method and compositions for detecting botulinum neurotoxin
ES2345506T3 (es) * 2005-04-05 2010-09-24 Allergan, Inc. Ensayos de fret a base de colorantes lipofilos para determinar la actividad de la toxina clostridial.
CN101932936B (zh) * 2007-09-14 2016-04-27 拜奥麦迪逊公司 利用切割序列和间隔子的共振能量转移测定

Also Published As

Publication number Publication date
EP2488868B1 (fr) 2016-03-30
PL2488868T3 (pl) 2016-09-30
EP2488868A1 (fr) 2012-08-22
LT3067696T (lt) 2018-09-10
US9453254B2 (en) 2016-09-27
EP3067696A1 (fr) 2016-09-14
ES2676277T3 (es) 2018-07-18
WO2011047265A1 (fr) 2011-04-21
ES2576747T3 (es) 2016-07-11
EP2488868A4 (fr) 2013-10-02
EP3067696B1 (fr) 2018-06-06
DK2488868T3 (en) 2016-05-09
US20120322092A1 (en) 2012-12-20

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