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WO2010133963A3 - Formation de combinaisons d'échantillons au moyen de systèmes à ponts liquides - Google Patents

Formation de combinaisons d'échantillons au moyen de systèmes à ponts liquides Download PDF

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Publication number
WO2010133963A3
WO2010133963A3 PCT/IB2010/001254 IB2010001254W WO2010133963A3 WO 2010133963 A3 WO2010133963 A3 WO 2010133963A3 IB 2010001254 W IB2010001254 W IB 2010001254W WO 2010133963 A3 WO2010133963 A3 WO 2010133963A3
Authority
WO
WIPO (PCT)
Prior art keywords
liquid bridge
forming sample
sample combinations
bridge systems
combinations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2010/001254
Other languages
English (en)
Other versions
WO2010133963A2 (fr
Inventor
Mark Davies
Tara Dalton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Priority to EP10749681A priority Critical patent/EP2432579A2/fr
Publication of WO2010133963A2 publication Critical patent/WO2010133963A2/fr
Publication of WO2010133963A3 publication Critical patent/WO2010133963A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00599—Solution-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00889—Mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G01N2035/00544—Mixing by a special element, e.g. stirrer using fluid flow

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Structural Engineering (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

L'invention concerne des procédés permettant d'élaborer des ponts liquides et des procédés permettant de former des combinaisons prédéterminées d'échantillons au moyen de ponts liquides.
PCT/IB2010/001254 2009-05-20 2010-05-10 Formation de combinaisons d'échantillons au moyen de systèmes à ponts liquides Ceased WO2010133963A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10749681A EP2432579A2 (fr) 2009-05-20 2010-05-10 Formation de combinaisons d'échantillons au moyen de systèmes à ponts liquides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/469,325 US8501497B2 (en) 2006-02-07 2009-05-20 Forming sample combinations using liquid bridge systems
US12/469,325 2009-05-20

Publications (2)

Publication Number Publication Date
WO2010133963A2 WO2010133963A2 (fr) 2010-11-25
WO2010133963A3 true WO2010133963A3 (fr) 2011-01-13

Family

ID=42988495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2010/001254 Ceased WO2010133963A2 (fr) 2009-05-20 2010-05-10 Formation de combinaisons d'échantillons au moyen de systèmes à ponts liquides

Country Status (3)

Country Link
US (2) US8501497B2 (fr)
EP (1) EP2432579A2 (fr)
WO (1) WO2010133963A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8298833B2 (en) 2006-02-07 2012-10-30 Stokes Bio Limited Liquid bridge and system
US8501497B2 (en) 2006-02-07 2013-08-06 Stokes Bio Limited Forming sample combinations using liquid bridge systems

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1663497B2 (fr) 2003-09-05 2020-03-25 Stokes Bio Limited Systeme d'analyse microfluide
US8735169B2 (en) 2006-02-07 2014-05-27 Stokes Bio Limited Methods for analyzing agricultural and environmental samples
GB0701641D0 (en) * 2007-01-29 2007-03-07 Iti Scotland Ltd Analyte manipulation and detection
WO2012031630A1 (fr) * 2010-09-09 2012-03-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Dispositif microfluidique, système de dosage microfluidique et procédé de mesure et de dosage d'un écoulement microfluidique
EP2694213B1 (fr) * 2011-04-08 2020-05-06 Stokes Bio Limited Système de détection biologique
EP2694212A1 (fr) 2011-04-08 2014-02-12 Stokes Bio Limited Système et procédé de charge de fluides
CN103733074B (zh) * 2011-04-08 2016-06-15 斯多克斯生物有限公司 用于连续流动式pcr系统的系统及方法
EP4026616B1 (fr) * 2015-08-26 2024-12-11 Emulate, Inc. Ensemble collecteur de perfusion
GB201518389D0 (en) * 2015-10-16 2015-12-02 Isis Innovation Interactions between immiscible fluids
CN106940948B (zh) * 2017-05-09 2022-09-20 临沂大学 一种高径比可变的液桥实验装置及其应用
CN116440969A (zh) * 2018-11-27 2023-07-18 斯蒂拉科技公司 具有优化的相流的微流控芯片架构

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1574586A2 (fr) * 2004-03-12 2005-09-14 Samsung Electronics Co., Ltd. Procédé et dispositif d'amplification d'acide nucléique
US20060257893A1 (en) * 2005-02-18 2006-11-16 Toru Takahashi Devices and methods for monitoring genomic DNA of organisms
WO2007091228A1 (fr) * 2006-02-07 2007-08-16 Stokes Bio Limited Pont liquide et système
WO2007091230A1 (fr) * 2006-02-07 2007-08-16 Stokes Bio Limited Système d'analyse microfluidique
WO2007091229A1 (fr) * 2006-02-07 2007-08-16 Stokes Bio Limited Réseau de mise en file d'attente de gouttelettes microfluidiques

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020182749A1 (en) 1999-05-11 2002-12-05 Aclara Biosciences, Inc. Sample evaporative control
US6193471B1 (en) 1999-06-30 2001-02-27 Perseptive Biosystems, Inc. Pneumatic control of formation and transport of small volume liquid samples
GB0026404D0 (en) * 2000-10-28 2000-12-13 Siddall & Hilton Ltd Body support arrangements
JP2004535273A (ja) * 2001-04-04 2004-11-25 アラダイアル， インコーポレイテッド 液体を分配するためのシステムおよび方法
FR2839504B1 (fr) * 2002-05-07 2004-06-18 Commissariat Energie Atomique Dispositif et procede de distribution de produits liquides
EP1663497B2 (fr) 2003-09-05 2020-03-25 Stokes Bio Limited Systeme d'analyse microfluide
US7655470B2 (en) 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US8735169B2 (en) 2006-02-07 2014-05-27 Stokes Bio Limited Methods for analyzing agricultural and environmental samples
US8501497B2 (en) 2006-02-07 2013-08-06 Stokes Bio Limited Forming sample combinations using liquid bridge systems
US8492168B2 (en) * 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
EP2069070B1 (fr) 2006-09-28 2013-11-27 Stokes Bio Limited Appareil d'analyse par pcr quantitative (qpcr)

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1574586A2 (fr) * 2004-03-12 2005-09-14 Samsung Electronics Co., Ltd. Procédé et dispositif d'amplification d'acide nucléique
US20060257893A1 (en) * 2005-02-18 2006-11-16 Toru Takahashi Devices and methods for monitoring genomic DNA of organisms
WO2007091228A1 (fr) * 2006-02-07 2007-08-16 Stokes Bio Limited Pont liquide et système
WO2007091230A1 (fr) * 2006-02-07 2007-08-16 Stokes Bio Limited Système d'analyse microfluidique
WO2007091229A1 (fr) * 2006-02-07 2007-08-16 Stokes Bio Limited Réseau de mise en file d'attente de gouttelettes microfluidiques

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8298833B2 (en) 2006-02-07 2012-10-30 Stokes Bio Limited Liquid bridge and system
US8501497B2 (en) 2006-02-07 2013-08-06 Stokes Bio Limited Forming sample combinations using liquid bridge systems
US9533304B2 (en) 2006-02-07 2017-01-03 Stokes Bio Limited Forming sample combinations using liquid bridge systems

Also Published As

Publication number Publication date
US9533304B2 (en) 2017-01-03
US8501497B2 (en) 2013-08-06
US20100029512A1 (en) 2010-02-04
US20140038196A1 (en) 2014-02-06
EP2432579A2 (fr) 2012-03-28
WO2010133963A2 (fr) 2010-11-25

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