WO2010127497A1 - Apparatus and method for detecting disease using dna database - Google Patents

Abstract

An apparatus and a method for detecting disease using a DNA database are disclosed. The apparatus comprises a reaction module at least preset the diabetes DNA database sequence information, a signal reaction detecting module and a processor module preset the status information. The reaction module, the signal reaction detecting module and the processor module are connected orderly. The reaction module is used for reacting with the DNA sample to be detected; the signal reaction detection module detects the status information of the reaction and feedbacks the status information to the processor module; and the processor module makes analysis according to the feedback status information and the preset status information. The apparatus and method could realize the high-throughput automation detection for diabetes.

Description

 Apparatus and method for detecting diseases using a DNA library

 [1] The present invention relates to an apparatus and method for detecting a disease.

 Background technique

 [2] The number of people with diabetes in China has ranked second in the world. In 2007, the epidemiological survey in China showed that the prevalence of diabetes was as high as 10.4%. Diabetes is also the third disease causing national death. Blood glucose testing is the key to the diagnosis and treatment of diabetes. Blood sugar refers to the concentration of glucose in the blood. Blood glucose can be measured by intravenous blood and capillary blood. The venous blood plasma glucose concentration test is mainly used for the diagnosis of diabetes. The miniature blood glucose meter for measuring capillary whole blood can be used for blood glucose monitoring in emergency patients and blood glucose self-monitoring in diabetic patients, and should not be used for diagnosis. At present, glucose measurement in clinical laboratories in China is mainly glucose oxidase (GOD) method, glucose hexokinase (HK) method and oxygen electrode rate method. However, the current blood glucose testing technology used in clinical practice has always stayed on the invasive method, and patients suffer from double traumatic pains of insulin injection and blood glucose testing.

 Disclosure of invention

 technical problem

 [3] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and to provide an apparatus and method capable of high-throughput detection of diseases.

 Technical solution

 [4] The technical solution adopted by the present invention to solve its technical problems is:

 [5] The device for measuring disease, comprising a reaction module at least preset with information about the sequence of the DNA library of the disease, a signal reaction detection module, and a processor module preset with state information, a reaction module, a signal reaction detection module, and a processor module connection, The reaction module is configured to react with the DNA sample to be tested, and the signal reaction detection module detects the state information of the reaction, and feeds the state information to the processor module, and the processor module analyzes according to the feedback state information and the preset state information.

[6] The reaction module comprises a DNA probe containing sequence information of a disease DNA library and a DNA probe containing control sequence information; the disease is diabetes, and the reaction module can include a DNA library sequence containing diabetes Information DNA probes and DNA probes containing control sequence information. The reaction module includes micropores (such as nanotubes and microtubules) having coordinates, and the microwells correspond one-to-one with the DNA probes. The reaction module further includes a reaction system including a DNA detection reaction solution and a washing solution. The signal reaction detection module is further connected with a sensor module. The reaction module is a DNA microarray chip comprising a DNA probe containing sequence information of a diabetes DNA library and a DNA probe containing control sequence information.

 [7] The signal reaction detection module includes an image sensor for acquiring an image of the reaction module. The processor module is further connected with a display module and an interface module.

 [8] A device for detecting diseases by using a DNA library, comprising a reaction module preset with diabetes DNA library sequence information and control sequence information, a signal reaction detection module, and a processor module preset with state information, a reaction module, a signal reaction The detecting module and the processor module are sequentially connected, the reaction module is configured to react with the DNA sample to be tested, the signal reaction detecting module detects the state information of the reaction, and feeds the state information to the processor module, and the processor module according to the state of the feedback Information and preset status information are analyzed. The reaction module further includes a reaction system including a DNA detection reaction solution and a washing solution, and the signal reaction detecting module is further connected with a sensor module for feeding back reaction system status information.

 [9] The reaction module has a plurality of coordinate points, and each coordinate point is provided with a sequence containing a DNA library of diabetes

DNA probe or DNA probe containing control sequence information. The reaction module has a plurality of micropores, each micropore corresponding to one coordinate, and each micropore has a DNA containing the DNA sequence information of the diabetes DNA or DNA containing the control sequence information.

 [10] The signal reaction detection module includes an image sensor for acquiring an image of the reaction module, the image sensor acquires image state information after the reaction, and the processor module determines image state information and preset image state information according to the reaction. Analyze.

 [11] A method for detecting a disease using a DNA library, comprising the steps of: a) distributing a sample of the DNA to be tested on the surface of the DNA microarray chip, and a preset DNA probe or micropore containing information on the sequence of the disease DNA library; DNA reaction; b) obtaining DNA sample and DNA to be tested

 Status information of the probe reaction; c) analysis based on the acquired status information and preset status information. In the step c), the judgment result is obtained by comparing the acquired state information with the preset state information, and the state information is selected from the group consisting of: color, brightness, current on and off, level.

Beneficial effect [12] The beneficial effects of the present invention are: 1) the reaction module reacts with the DNA sample to be tested, the signal reaction detection module detects the reaction state, and the processor module compares the state information and obtains the analysis result, thereby achieving high diabetes Automated testing of flux; 2) After performing diabetes testing, the device is able to use the latest genetic information to detect and assess the health and genetic risk of the subject.

 DRAWINGS

 [13] FIG. 1 is a structural block diagram of an apparatus for detecting a disease using a DNA library in the present embodiment;

Fig. 2 is a schematic structural view of a DNA microarray chip of the present embodiment.

 Embodiments of the invention

 [15] As shown in FIG. 1 and FIG. 2, an apparatus for detecting diseases by using a DNA library includes a reaction module, a sensor module, a signal reaction detection module, a processor module, a display module, and an interface module. The reaction module and the sensor module are all connected to the signal reaction detection module; the signal reaction detection module is connected to the processor module; the display module and the interface module are all connected to the processor module. The reaction module is preset with disease DNA library sequence information and control sequence information. The sample liquid to be tested is evenly distributed on the surface of the reaction module, and the signal reaction detection module detects the reaction state of the reaction module and the sample to be tested and the feedback information of the sensor module, and is sent to the processor module after being preprocessed. The processor module processes and analyzes the result, and sends the calculated result value to the display module for display or to the interface module output.

 [16] The sample to be tested is taken from the blood or other tissues of the object to be tested (such as hair follicles, etc.), and after special treatment, a DNA sample with better detection characteristics is obtained, and the special treatment is coupled with a fluorescent dye as in the prior art.

 [17] The reaction module includes a reaction system, at least one DNA probe containing information on the sequence of the disease DNA library, and at least one DNA probe containing information on the control sequence, and each disease DNA library sequence information corresponds to a control sequence information. Each DNA probe corresponds to a coordinate, and the DNA probe can be coupled to fluorescein, biotin or other label. The reaction system includes a DNA detection reaction solution and a wash solution.

 [18] The signal response detection module includes an image sensor with a microscope head (eg CCD, CMOS)

 The sensor is input to the processor module after pre-processing (such as photoelectric conversion, AD analog-to-digital conversion), and the converted value of the temperature, special ion, humidity, etc. of the reaction system fed back by the sensor module is preprocessed and transmitted to the processor. Module.

 [19] The sensor module monitors the temperature, specific ions, humidity, etc. of the reaction system of the reaction module.

[20] The processor module can be an embedded or PC hardware and software system architecture, which can respond to the signal detection module and transmit The information transmitted by the sensor module is analyzed and processed. The processor module can be built-in image recognition and processing software module, and complete the coordinate confirmation, brightness recognition, color recognition, or check the current on and off, the level of the image of the reaction module to achieve qualitative analysis, and if there is brightness, the coordinates are indicated. The DNA of the spot reacts with the sample to be tested. The DNA sequence consists of four bases, A, T, C, and G, which are paired with 1\C and G. The DNA hybridization reaction is described below as an example. As shown in Figure 2, the DNA sequence of the A-coordinate DNA probe is shown. The DNA sequence of the AATTCG, M coordinate DNA probe is TGCGA. If the A coordinate has brightness, it means there is a complementary sequence (TTAAGC); if the M point has no brightness, it means there is no complementary DNA sequence that reacts with it. (ACGCT). The DNA probe containing the control sequence information of the reaction module is a DNA probe of a normal gene.

 [21] The display module is used to display various types of displays such as CRT, LCD, LED, etc. The interface module is used to realize the signal connection between the entire detecting device and the outside world, such as various serial communication and network interfaces.

 [22] Taking diabetes as an example to illustrate the device and method for detecting diseases using a DNA library:

 [23] Screen differentially expressed genes between normal blood glucose and abnormal blood glucose, select N diabetes DNA library sequence information (including polygenic mutations and SNP locus sequences that cause diabetes), and customize homologous DNA library sequence information and control sequence information. Reaction module.

 [24] Extract the tissue containing DNA genetic information, such as blood or hair follicles, to be tested, and then make it into a DNA sample with good detection characteristics, and then fully mix the DNA sample with the coordinates of the reaction module. The DNA probe is hybridized, and after washing to remove the unreacted DNA sample, the reaction signal is sent to the processor module via the signal reaction detection module. The processor module converts the reaction signal into an interpretable signal for qualitative analysis of the reaction between the DNA library sequence information and the sample to be tested in the reaction module, thereby interpreting the health level and genetic background of the object to be tested.

[25] In the present embodiment, the DNA library sequence information includes a polygenic mutation and a SNP site sequence of diabetes, and the reaction module includes a DNA probe of a plurality of normal genes as an analysis control, and the reaction between the sample to be tested and the DNA probe is performed. To qualitatively analyze the sample to be tested. The reaction module is a DNA microarray chip having a plurality of DNA probes. After the reaction module reacts with the DNA sample to be tested, the state information (such as color, brightness, gray level, current, level) of the reaction module changes, such as the color of the image before the reaction module reacts, the brightness, and the color of the image after the reaction. The brightness is different, the current on/off state or the level is different; the image of the reaction module and the state information of the reaction module are obtained by the signal reaction detection module and fed back to the processor module, The processor module compares the status information with the preset status information.

 An analysis of health and genetic risks can be made. At the same time, since each DNA sequence of the reaction module has its own coordinates, it is possible to visually determine which gene of the patient is mutated by changing the state information of the coordinate (for example, the DNA probe changes from no brightness to brightness). The reaction module may include only a DNA probe containing information on the sequence of the diabetes DNA library, or a DNA probe containing information on the sequence of the DNA library of the diabetes and a DNA probe containing information on the control sequence.

[26] A method of detecting a disease using a DNA library, comprising the steps of:

 [27] a) uniformly distributing the sample to be tested (which can be coupled with the fluorescent dye) to the surface of the reaction module, and reacting with the DNA of the preset DNA probe containing the sequence information of the diabetic DNA library or the micropore; b The signal reaction detection module sends the detected reaction module and the reaction state information of the sample to be tested, and the information (temperature, special ions, humidity, etc.) fed back by the sensor module to the processor module, and the processor module calculates The resulting value is displayed by the display module or output via the interface module.

 [28] The device and method for detecting diseases using the DNA library of the present invention can be used not only for the detection of diabetes, but also for coronary heart disease or other diseases which can be detected by using DNA.

 The above is a further detailed description of the present invention in conjunction with the specific preferred embodiments. It is not intended that the specific embodiments of the invention are limited to the description. It will be apparent to those skilled in the art that the present invention may be practiced without departing from the spirit and scope of the invention.

Claims

Claim  [1] A device for detecting diseases by using a DNA library, comprising: a reaction module including at least a sequence information of a disease DNA library, a signal reaction detection module, and a processor module preset with state information, a reaction module, a signal The reaction detection module and the processor module are connected, the reaction module is configured to react with the DNA sample to be tested, the signal reaction detection module detects the state information of the reaction, and feeds the state information to the processor module, and the processor module according to the feedback state information and Preset status information is analyzed.  [2] The apparatus for detecting a disease using a DNA library according to claim 1, wherein the reaction module comprises a DNA probe containing sequence information of a disease DNA library and a DAA probe containing control sequence information.  [3] The apparatus for detecting a disease using a DNA library according to claim 2, wherein the reaction module comprises micropores having coordinates, and the micropores are in one-to-one correspondence with the DNA probes. [4] The apparatus for detecting a disease using a DNA library according to claim 1, wherein the reaction module further comprises a reaction system comprising a DNA detection reaction solution and a washing solution. [5] The apparatus for detecting a disease using a DNA library according to claim 5, wherein: the signal reaction detecting module is further connected to the sensor module. [6] The apparatus for detecting diseases by using a DNA library according to claim 1, wherein: the reaction module is a DNA microarray chip, and the DNA microarray chip includes a DNA probe containing sequence information of a disease DNA library and contains DNA probe for control sequence information. [7] The apparatus for detecting a disease using a DNA library according to claim 1, wherein: the signal reaction detecting module includes an image sensor for acquiring an image of the reaction module. [8] The apparatus for detecting a disease using a DNA library according to claim 7, wherein: the processor module is further connected with a display module and an interface module. [9] The apparatus for detecting diseases using DNA according to any one of claims 1 to 8, characterized in that : The disease is diabetes. [10] A device for detecting diseases by using a DNA library, comprising: a reaction module preset with diabetes DNA library sequence information and control sequence information, a signal reaction detection module, and a processor module preset with state information, reaction The module, the signal reaction detection module and the processor module are connected in sequence, The reaction module is configured to react with the DNA sample to be tested, and the signal reaction detection module detects the state information of the reaction, and feeds the state information to the processor module, and the processor module performs analysis according to the feedback state information and the preset state information.  [11] The apparatus for detecting a disease using a DNA library according to claim 10, wherein: the reaction module further comprises a reaction system, the reaction system comprising a DNA detection reaction solution and a washing solution, and the signal reaction detecting module further A sensor module for feeding back reaction system status information is connected.  [12] The apparatus for detecting a disease using a DNA library according to claim 11, wherein: the reaction module has a plurality of coordinate points, and each coordinate point is provided with a DNA probe containing a sequence information of a diabetes DNA library or A DNA probe containing control sequence information.  [13] The apparatus for detecting a disease using a DNA library according to claim 11, wherein: the reaction module has a plurality of micropores, each micropore corresponding to one coordinate, and each micropore has a DNA containing diabetes DNA of library sequence information or DNA containing control sequence information.  [14] The apparatus for detecting a disease using a DNA library according to any one of claims 10 to 13, wherein: the signal reaction detecting module includes an image sensor for acquiring an image of a reaction module, the image sensor acquiring The image state information after the reaction is analyzed by the processor module according to the image state information after the reaction and the preset image state information.  [15] A method for detecting a disease using a DNA library, comprising: the steps of:  a) distributing the DNA sample to be tested on the surface of the DNA microarray chip, and reacting with the DNA probe or the DNA in the microwell containing the sequence information of the disease DNA library;  b) obtaining state information of the reaction of the DNA sample to be tested with the DNA probe or the DNA in the micropore; c) performing analysis according to the acquired state information and preset state information.  [16] The method for detecting a disease using a DNA library according to claim 15, wherein: in the step c), the judgment result is obtained by comparing the acquired state information with the preset state information, and the state information is selected from the group consisting of : Color, brightness, current on and off, high and low levels.  [17] A method for detecting a disease using a DNA library according to claim 15 or 16, wherein the disease is diabetes.