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WO2010127324A3 - Chimeric pdz domain-containing protein for viral detection - Google Patents

Chimeric pdz domain-containing protein for viral detection Download PDF

Info

Publication number
WO2010127324A3
WO2010127324A3 PCT/US2010/033308 US2010033308W WO2010127324A3 WO 2010127324 A3 WO2010127324 A3 WO 2010127324A3 US 2010033308 W US2010033308 W US 2010033308W WO 2010127324 A3 WO2010127324 A3 WO 2010127324A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
pathogen
pdz domain
human
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/033308
Other languages
French (fr)
Other versions
WO2010127324A2 (en
Inventor
Michael P. Belmares
Johannes Schweizer
Peter Lu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Arbor Vita Corp
Original Assignee
Becton Dickinson and Co
Arbor Vita Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co, Arbor Vita Corp filed Critical Becton Dickinson and Co
Publication of WO2010127324A2 publication Critical patent/WO2010127324A2/en
Publication of WO2010127324A3 publication Critical patent/WO2010127324A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention provides compositions and methods for detecting pathogen infections in human samples and purifying pathogen proteins from human samples. The detection and purification utilize specific chimeric PDZ domain-containing proteins to detect the presence of pathogen proteins or abnormal expression of human proteins resulting from pathogen infections, and purify such pathogen proteins. Specific methods, compositions, and kits are disclosed herein for the detection and purification of oncogenic human papilloma virus E6 proteins in clinical samples.
PCT/US2010/033308 2009-04-30 2010-04-30 Chimeric pdz domain-containing protein for viral detection Ceased WO2010127324A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17441609P 2009-04-30 2009-04-30
US61/174,416 2009-04-30

Publications (2)

Publication Number Publication Date
WO2010127324A2 WO2010127324A2 (en) 2010-11-04
WO2010127324A3 true WO2010127324A3 (en) 2010-12-23

Family

ID=43032815

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/033308 Ceased WO2010127324A2 (en) 2009-04-30 2010-04-30 Chimeric pdz domain-containing protein for viral detection

Country Status (1)

Country Link
WO (1) WO2010127324A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015097268A1 (en) * 2013-12-23 2015-07-02 Universite De Strasbourg A chimeric construct for diagnosis and therapy of papillomavirus-induced cancers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070292899A1 (en) * 2006-05-11 2007-12-20 Stephen Lovell Method Of Protein Extraction From Cells
US20080124702A1 (en) * 2001-02-16 2008-05-29 Lu Peter S Methods of diagnosing cervical cancer
WO2009006611A1 (en) * 2007-07-03 2009-01-08 Arbor Vita Corporation Small molecule inhibitors of pdz interactions

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124702A1 (en) * 2001-02-16 2008-05-29 Lu Peter S Methods of diagnosing cervical cancer
US20070292899A1 (en) * 2006-05-11 2007-12-20 Stephen Lovell Method Of Protein Extraction From Cells
WO2009006611A1 (en) * 2007-07-03 2009-01-08 Arbor Vita Corporation Small molecule inhibitors of pdz interactions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NOURRY ET AL.: "PDZ Domain Proteins: Plug and Play!", SCIENCE'S STKE, 2003, pages 1 - 12, Retrieved from the Internet <URL:www.stke.org/cgi/content/full/sigtrans;2003/179/re7> *

Also Published As

Publication number Publication date
WO2010127324A2 (en) 2010-11-04

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