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WO2010115912A3 - Nucleic acid sequences based on the bacterial ssra gene and its tmrna transcript for the molecular detection of s.aureus - Google Patents

Nucleic acid sequences based on the bacterial ssra gene and its tmrna transcript for the molecular detection of s.aureus Download PDF

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Publication number
WO2010115912A3
WO2010115912A3 PCT/EP2010/054570 EP2010054570W WO2010115912A3 WO 2010115912 A3 WO2010115912 A3 WO 2010115912A3 EP 2010054570 W EP2010054570 W EP 2010054570W WO 2010115912 A3 WO2010115912 A3 WO 2010115912A3
Authority
WO
WIPO (PCT)
Prior art keywords
bacterial
aureus
nucleic acid
acid sequences
sequences based
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2010/054570
Other languages
French (fr)
Other versions
WO2010115912A2 (en
Inventor
Terry James Smith
Majella Maher
Justin O Grady
Thomas Barry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National University of Ireland Galway NUI
Original Assignee
National University of Ireland Galway NUI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University of Ireland Galway NUI filed Critical National University of Ireland Galway NUI
Publication of WO2010115912A2 publication Critical patent/WO2010115912A2/en
Publication of WO2010115912A3 publication Critical patent/WO2010115912A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A diagnostic kit for a bacterial species comprising an oligonucleotide probe capable of binding to at least a portion of the ssrA gene or its corresponding tRNA. The kit may comprise a primer for amplification of at least a portion of the ssrA gene.
PCT/EP2010/054570 2009-04-09 2010-04-07 Nucleic acid sequences based on the bacterial ssra gene and its tmrna transcript for the molecular detection of s.aureus Ceased WO2010115912A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IE2009/0288 2009-04-09
IE20090288 2009-04-09

Publications (2)

Publication Number Publication Date
WO2010115912A2 WO2010115912A2 (en) 2010-10-14
WO2010115912A3 true WO2010115912A3 (en) 2010-12-02

Family

ID=42224103

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/054570 Ceased WO2010115912A2 (en) 2009-04-09 2010-04-07 Nucleic acid sequences based on the bacterial ssra gene and its tmrna transcript for the molecular detection of s.aureus

Country Status (1)

Country Link
WO (1) WO2010115912A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000070086A1 (en) * 1999-05-14 2000-11-23 Enterprise Ireland (Trading As Bioresearch Ireland) Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms
US20060216733A1 (en) * 1999-04-07 2006-09-28 University Of Utah Research Foundation Eubacterial tmRNA sequences and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060216733A1 (en) * 1999-04-07 2006-09-28 University Of Utah Research Foundation Eubacterial tmRNA sequences and uses thereof
WO2000070086A1 (en) * 1999-05-14 2000-11-23 Enterprise Ireland (Trading As Bioresearch Ireland) Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
DIMOV IVAN K ET AL: "Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics.", LAB ON A CHIP DEC 2008 LNKD- PUBMED:19023470, vol. 8, no. 12, December 2008 (2008-12-01), pages 2071 - 2078, XP002586214, ISSN: 1473-0197 *
GLYNN BARRY ET AL: "Current and emerging molecular diagnostic technologies applicable to bacterial food safety", INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, vol. 59, no. 2, May 2006 (2006-05-01), pages 126 - 139, XP002586215, ISSN: 1364-727X *
LASLETT DEAN ET AL: "ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences.", NUCLEIC ACIDS RESEARCH, vol. 32, no. 1, 2004, pages 11 - 16, XP002586217, ISSN: 0305-1048 *
MIGNARD SOPHIE ET AL: "Identification of Mycobacterium using the EF-Tu encoding (tuf) gene and the tmRNA encoding (ssrA) gene", JOURNAL OF MEDICAL MICROBIOLOGY, vol. 56, no. 8, August 2007 (2007-08-01), pages 1033 - 1041, XP002586216, ISSN: 0022-2615 *
O' GRADY ET AL: "Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target", FOOD MICROBIOLOGY, ACADEMIC PRESS LTD, LONDON, GB LNKD- DOI:10.1016/J.FM.2007.07.007, vol. 25, no. 1, 6 August 2007 (2007-08-06), pages 75 - 84, XP022336928, ISSN: 0740-0020 *
O'GRADY JUSTIN ET AL: "tmRNA - a novel high-copy-number RNA diagnostic target - its application for Staphylococcus aureus detection using real-time NASBA", FEMS MICROBIOLOGY LETTERS, vol. 301, no. 2, December 2009 (2009-12-01), pages 218 - 223, XP002586218, ISSN: 0378-1097 *
SCHELER OTT ET AL: "Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications.", BMC BIOTECHNOLOGY 2009 LNKD- PUBMED:19445684, vol. 9, 2009, pages 45, XP002586219, ISSN: 1472-6750 *
WERNECKE MARTINA ET AL: "Evaluation of a novel real-time PCR test based on the ssrA gene for the identification of group B streptococci in vaginal swabs.", BMC INFECTIOUS DISEASES 2009 LNKD- PUBMED:19732424, vol. 9, 2009, pages 148, XP002586220, ISSN: 1471-2334 *

Also Published As

Publication number Publication date
WO2010115912A2 (en) 2010-10-14

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