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WO2010108005A3 - Novel neural progenitors from pluripotent stem cells, methods of producing same and use to produce neural cells - Google Patents

Novel neural progenitors from pluripotent stem cells, methods of producing same and use to produce neural cells Download PDF

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Publication number
WO2010108005A3
WO2010108005A3 PCT/US2010/027819 US2010027819W WO2010108005A3 WO 2010108005 A3 WO2010108005 A3 WO 2010108005A3 US 2010027819 W US2010027819 W US 2010027819W WO 2010108005 A3 WO2010108005 A3 WO 2010108005A3
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WIPO (PCT)
Prior art keywords
cells
stem cells
neural
pluripotent stem
novel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/027819
Other languages
French (fr)
Other versions
WO2010108005A2 (en
Inventor
David Reynolds
Thomas Schulz
Allan Robins
Stephen Dalton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Georgia Research Foundation Inc UGARF
Viacyte Inc
Original Assignee
University of Georgia Research Foundation Inc UGARF
Novocell Inc
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Filing date
Publication date
Application filed by University of Georgia Research Foundation Inc UGARF, Novocell Inc filed Critical University of Georgia Research Foundation Inc UGARF
Publication of WO2010108005A2 publication Critical patent/WO2010108005A2/en
Publication of WO2010108005A3 publication Critical patent/WO2010108005A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/195—Heregulin, neu differentiation factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to the discovery of a novel multipotent stem cell termed pre-rosette stage ectoderm progenitor cells or BSC cells. These cells may be isolated, grown stably for a number of which are novel, self-renewing early ectodermal cell types (pre-rosette stage ectoderm progenitors) exhibiting neural progenitor-like characteristics generations and cryopreserved for stable storage. These cells may be further differentiated into neural cells such as neurons and glia cells, which may be used for transplantation and other therapies. Methods of producing these cells from pluripotent stem cells, including human embryonic stem cells, umbilical/placental blood cord stem cells or induced pluripotent stem cells (iPS cells or iPSCs), including reprogrammed adult progenitor cells, are also disclosed.
PCT/US2010/027819 2009-03-18 2010-03-18 Novel neural progenitors from pluripotent stem cells, methods of producing same and use to produce neural cells Ceased WO2010108005A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21037109P 2009-03-18 2009-03-18
US61/210,371 2009-03-18

Publications (2)

Publication Number Publication Date
WO2010108005A2 WO2010108005A2 (en) 2010-09-23
WO2010108005A3 true WO2010108005A3 (en) 2011-03-31

Family

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PCT/US2010/027819 Ceased WO2010108005A2 (en) 2009-03-18 2010-03-18 Novel neural progenitors from pluripotent stem cells, methods of producing same and use to produce neural cells

Country Status (1)

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WO (1) WO2010108005A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11261425B2 (en) 2009-08-12 2022-03-01 Kyoto University Method for inducing differentiation of pluripotent stem cells into neural precursor cells
CA2770753C (en) 2009-08-12 2019-01-15 Kyoto University Method for inducing differentiation of pluripotent stem cells into neural precursor cells
WO2012058243A2 (en) 2010-10-26 2012-05-03 Case Western Reserve University Cell fate conversion of differentiated somatic cells into glial cells
EP2633036A4 (en) * 2010-10-26 2013-12-04 Univ Case Western Reserve METHODS OF DIFFERENTIATION FOR THE PRODUCTION OF GLIAL CELL POPULATIONS
KR20130101557A (en) 2010-11-02 2013-09-13 고꾸리쯔다이가꾸호오진 구마모또 다이가꾸 Method for producing intestinal cells
EP2658966B1 (en) * 2010-12-31 2017-04-05 University of Georgia Research Foundation, Inc. Differentiation of human pluripotent stem cells to multipotent neural crest cells
WO2012174467A2 (en) * 2011-06-15 2012-12-20 Salk Institute For Biological Studies Cord blood-derived neurons by expression of sox2
TWI493034B (en) * 2011-12-14 2015-07-21 Nat Univ Chung Hsing Neuronal epithelial cells differentiated by universal stem cells and the medium used and their differentiation methods
US9074186B2 (en) 2012-08-15 2015-07-07 Boston Medical Center Corporation Production of red blood cells and platelets from stem cells
US9730975B2 (en) 2014-11-25 2017-08-15 The Penn State Research Foundation Chemical reprogramming of human glial cells into neurons for brain and spinal cord repair
CN106434535B (en) * 2015-08-11 2021-05-14 中国科学院广州生物医药与健康研究院 Method for controlling stem cell differentiation
US12209253B2 (en) * 2016-08-29 2025-01-28 EMULATE, Inc. Development of spinal cord on a microfluidic chip
ES2626491B1 (en) 2015-12-03 2018-06-25 Consejo Superior De Investigaciones Científicas (Csic) MONOCLONAL ANTIBODIES AGAINST BAMBI AND USE FOR TREATMENT OF INFLAMMATORY DISEASES
WO2017123806A1 (en) 2016-01-12 2017-07-20 Cedars-Sinai Medical Center A method of non destructive monitoring of biological processes in microfluidic tissue culture systems
WO2017136462A2 (en) 2016-02-01 2017-08-10 EMULATE, Inc. Systems and methods for growth of intestinal cells in microfluidic devices
US11913022B2 (en) 2017-01-25 2024-02-27 Cedars-Sinai Medical Center In vitro induction of mammary-like differentiation from human pluripotent stem cells
US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
US11414648B2 (en) 2017-03-24 2022-08-16 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium
US12161676B2 (en) 2018-03-23 2024-12-10 Cedars-Sinai Medical Center Methods of use of islet cells
US11981918B2 (en) 2018-04-06 2024-05-14 Cedars-Sinai Medical Center Differentiation technique to generate dopaminergic neurons from induced pluripotent stem cells
EP3775161A4 (en) 2018-04-06 2022-04-06 Cedars-Sinai Medical Center NEURODEGENERATIVE DISEASE MODELS DERIVED FROM HUMAN PLURIPOTENT STEM CELLS ON A MICROFLUIDIC CHIP

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHAMBERS, S.M. ET AL.: "Highly efficient neural conversion og human ES and iPS cells by dual inhibition of SMAD signaling.", NATURE BIOTECHNOLOGY, vol. 27, no. 3, March 2009 (2009-03-01), pages 275 - 280 *
ELKABETZ, Y. ET AL.: "Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.", GENES DEV., vol. 22, 2008, pages 152 - 165 *
PATAPOUTIAN, A. ET AL.: "Roles of Wnt proteins in neural development and maintenance.", CURRENT OPINION IN NEUROBIOLOGY, vol. 10, 2000, pages 392 - 399 *
SMITH, J.R. ET AL.: "Inhibition of activin/nodal signaling promotes specification of human embryonic stem cells into neuroectoderm.", DEVELOPMENTAL BIOLOGY, vol. 313, 2008, pages 107 - 117 *
ZANG, Y. ET AL.: "AMP-activated protein kinase is involved in neural stem cell growth suppression and cell cycle arrest by 5-aminoimidazole-4- carboxamide-1-beta-D-ribofuranoside and glucose deprivation by down-regulating phospho-retinoblastoma protein and cyclin D.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, no. 10, 6 March 2009 (2009-03-06), pages 6175 - 6184 *

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Publication number Publication date
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