[go: up one dir, main page]

WO2010105020A3 - Baculovirus avec production de virions améliorée et procédé pour la production de baculovirus - Google Patents

Baculovirus avec production de virions améliorée et procédé pour la production de baculovirus Download PDF

Info

Publication number
WO2010105020A3
WO2010105020A3 PCT/US2010/026922 US2010026922W WO2010105020A3 WO 2010105020 A3 WO2010105020 A3 WO 2010105020A3 US 2010026922 W US2010026922 W US 2010026922W WO 2010105020 A3 WO2010105020 A3 WO 2010105020A3
Authority
WO
WIPO (PCT)
Prior art keywords
vsv
baculoviruses
production
amino acids
retroviral vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/026922
Other languages
English (en)
Other versions
WO2010105020A2 (fr
Inventor
Paul Mccray
Patrick Sinn
Gary Blissard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Iowa Research Foundation UIRF
Boyce Thompson Institute
Original Assignee
University of Iowa Research Foundation UIRF
Boyce Thompson Institute for Plant Research Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Iowa Research Foundation UIRF, Boyce Thompson Institute for Plant Research Inc filed Critical University of Iowa Research Foundation UIRF
Publication of WO2010105020A2 publication Critical patent/WO2010105020A2/fr
Publication of WO2010105020A3 publication Critical patent/WO2010105020A3/fr
Priority to US13/231,324 priority Critical patent/US8703469B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention porte sur un procédé de co-expression d'une partie du gène de protéine G VSV ou d'une partie « de tige » tronquée par GP64 et d'un rétrovirus, lequel procédé augmente le titre de vecteurs rétroviraux. Une protéine G VSV tronquée, de préférence composée d'un petit segment à partir de la partie C terminale de l'ectodomaine plus les domaines transmembranaires (TM) et de queue cytoplasmique (CTD) de G VSV, co-exprimée par des vecteurs rétroviraux, améliore les titres de production des vecteurs rétroviraux. Un mode de réalisation préféré utilise un produit de construction G VSV qui comprend un épitope c-Myc N terminal plus 42 d'acides aminés provenant de la partie C terminale de l'ectodomaine, 20 acides aminés provenant du domaine TM prédit et 29 acides aminés provenant du CTD prédit de la protéine G VSV.
PCT/US2010/026922 2007-07-06 2010-03-11 Baculovirus avec production de virions améliorée et procédé pour la production de baculovirus Ceased WO2010105020A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/231,324 US8703469B2 (en) 2007-07-06 2011-09-13 Baculoviruses with enhanced virion production and a method for the production of baculoviruses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15994009P 2009-03-13 2009-03-13
US61/159,940 2009-03-13

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2008/069132 Continuation-In-Part WO2009014878A2 (fr) 2007-07-06 2008-07-03 Baculovirus avec production de virion améliorée, et procédé de production de baculovirus
US12/667,956 Continuation-In-Part US8961951B2 (en) 2007-07-06 2008-07-03 Baculoviruses with enhanced virion production and a method for the production of baculoviruses

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/231,324 Continuation US8703469B2 (en) 2007-07-06 2011-09-13 Baculoviruses with enhanced virion production and a method for the production of baculoviruses

Publications (2)

Publication Number Publication Date
WO2010105020A2 WO2010105020A2 (fr) 2010-09-16
WO2010105020A3 true WO2010105020A3 (fr) 2011-01-13

Family

ID=42729091

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/026922 Ceased WO2010105020A2 (fr) 2007-07-06 2010-03-11 Baculovirus avec production de virions améliorée et procédé pour la production de baculovirus

Country Status (1)

Country Link
WO (1) WO2010105020A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107287217A (zh) * 2017-06-30 2017-10-24 浙江大学 CvBV29‑1基因在降低果蝇免疫力和制备免疫低下型果蝇模型中的应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119548525B (zh) * 2024-11-29 2025-07-25 安徽农业大学 含锰的化合物在制备防治家蚕核型多角体病毒药物中的应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512421A (en) * 1991-02-19 1996-04-30 The Regents Of The University Of California Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors
US6410316B1 (en) * 1999-03-15 2002-06-25 Chiron Corporation Methods for producing a vector producing cell line utilizing a high multiplicity of transduction
WO2005038035A2 (fr) * 2003-10-15 2005-04-28 University Of Iowa Research Foundation Procedes de production et d'utilisation de retrovirus pseudotypes in vivo
WO2009014878A2 (fr) * 2007-07-06 2009-01-29 Boyce Thompson Institute For Plant Research Baculovirus avec production de virion améliorée, et procédé de production de baculovirus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512421A (en) * 1991-02-19 1996-04-30 The Regents Of The University Of California Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors
US6410316B1 (en) * 1999-03-15 2002-06-25 Chiron Corporation Methods for producing a vector producing cell line utilizing a high multiplicity of transduction
WO2005038035A2 (fr) * 2003-10-15 2005-04-28 University Of Iowa Research Foundation Procedes de production et d'utilisation de retrovirus pseudotypes in vivo
WO2009014878A2 (fr) * 2007-07-06 2009-01-29 Boyce Thompson Institute For Plant Research Baculovirus avec production de virion améliorée, et procédé de production de baculovirus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107287217A (zh) * 2017-06-30 2017-10-24 浙江大学 CvBV29‑1基因在降低果蝇免疫力和制备免疫低下型果蝇模型中的应用

Also Published As

Publication number Publication date
WO2010105020A2 (fr) 2010-09-16

Similar Documents

Publication Publication Date Title
ES2847698T3 (es) Construcción de ácido nucleico
EA201000077A1 (ru) Вирус гриппа с нарушенной репликацией для экспрессии гетерологичных последовательностей
WO2008114149A3 (fr) Antigènes chimériques
NZ595736A (en) Chimeric viruses presenting non-native surface proteins and uses thereof
IL313003A (en) Tigit- and light-based chimeric proteins
WO2007100584A3 (fr) Agents antiviraux et vaccins antigrippaux
PE20090683A1 (es) Peptidos de senalizacion
WO2010082804A3 (fr) Méthode de production d'une protéine ou d'un peptide physiologiquement actif à l'aide d'un fragment d'immunoglobuline
WO2012142515A3 (fr) Protéines hybrides fc comprenant de nouveaux lieurs ou agencements
PE20090225A1 (es) Proteinas de fusion que degradan el peptido beta amiloide
WO2008150101A3 (fr) Polypeptide chimérique comportant une protéine adhésive de la moule et une matrice extracellulaire
WO2009133462A3 (fr) Protéines
WO2009142460A3 (fr) Corps synergique fusionné anticorps-peptide
EA201991918A1 (ru) Самосборные белковые наночастицы со встроенными белками с шестиспиральным пучком
WO2018129200A3 (fr) Agonistes du récepteur pac1 (maxcaps) et leurs utilisations
WO2007011975A3 (fr) Methodes d'expression de proteines de recombinaison a partir de vecteurs lentiviraux
WO2009099777A3 (fr) Protéines hybrides vih chimèriques utilisées en tant que vaccins
HK1215441A1 (zh) 人血清白蛋白结合化合物及其融合蛋白
NZ586074A (en) LCLRP peptides, constructs and uses thereof
WO2008113536A8 (fr) Peptides neurotrophiques
WO2010105020A3 (fr) Baculovirus avec production de virions améliorée et procédé pour la production de baculovirus
CO6382184A2 (es) Proteinas fijadoras de receptores de interleukinas-21
ES2585185T3 (es) Polipéptidos quiméricos y sus aplicaciones terapéuticas contra una infección por Flaviviridae
WO2007047504A3 (fr) Proteines hybrides de transferrine modifiees par des peptides natriuretiques
WO2010057242A3 (fr) Vaccin

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10751395

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 10751395

Country of ref document: EP

Kind code of ref document: A2