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WO2010039941A3 - Dispositif de détection par nanocapteur biologique - Google Patents

Dispositif de détection par nanocapteur biologique Download PDF

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Publication number
WO2010039941A3
WO2010039941A3 PCT/US2009/059208 US2009059208W WO2010039941A3 WO 2010039941 A3 WO2010039941 A3 WO 2010039941A3 US 2009059208 W US2009059208 W US 2009059208W WO 2010039941 A3 WO2010039941 A3 WO 2010039941A3
Authority
WO
WIPO (PCT)
Prior art keywords
bio
nanosensor
dna
disease
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/059208
Other languages
English (en)
Other versions
WO2010039941A2 (fr
WO2010039941A8 (fr
WO2010039941A9 (fr
Inventor
Saion Kumar Sinha
Evan Terezia Sapi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NEW HAVEN, University of
Original Assignee
NEW HAVEN, University of
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NEW HAVEN, University of filed Critical NEW HAVEN, University of
Priority to CA2739370A priority Critical patent/CA2739370A1/fr
Priority to AU2009298497A priority patent/AU2009298497B2/en
Priority to US13/063,623 priority patent/US9919922B2/en
Priority to EP09818504.4A priority patent/EP2334811B1/fr
Publication of WO2010039941A2 publication Critical patent/WO2010039941A2/fr
Publication of WO2010039941A3 publication Critical patent/WO2010039941A3/fr
Publication of WO2010039941A9 publication Critical patent/WO2010039941A9/fr
Anticipated expiration legal-status Critical
Publication of WO2010039941A8 publication Critical patent/WO2010039941A8/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • General Physics & Mathematics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un dispositif de détection d'acide nucléique et un procédé qui fait appel à la technologie des nanocapteurs biologiques pour détecter un ADN duplex. Le dispositif est particulièrement applicable à la détection de la présence ou de l'absence d'un ADN duplex et à sa corrélation avec le diagnostic de maladies infectieuses. Dans un mode de réalisation, la maladie infectieuse est la maladie de Lyme ou une infection bactérienne ou virale. Le dispositif comprend un élément de nanocapteur biologique comprenant des nanotubes amorcés avec un ADN simple brin, à paroi simple ou à parois multiples. Le procédé consiste à mettre en contact l'élément de nanocapteur biologique avec une solution à analyser contenant potentiellement l'ADN d'intérêt. L'ADN d'intérêt qui s'hybride à l'ADN simple brin entraîne une modification mesurable des propriétés électriques du nanocapteur biologique. Les corrélations entre les résultats fournis par le dispositif et la présence d'états pathologiques peuvent déboucher sur un diagnostic rapide de maladies telles que la maladie de Lyme ou d'infections d'origine alimentaire telles que la salmonellose.
PCT/US2009/059208 2008-10-02 2009-10-01 Dispositif de détection par nanocapteur biologique Ceased WO2010039941A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2739370A CA2739370A1 (fr) 2008-10-02 2009-10-01 Dispositif de detection par nanocapteur biologique
AU2009298497A AU2009298497B2 (en) 2008-10-02 2009-10-01 Bionanosensor detection device
US13/063,623 US9919922B2 (en) 2008-10-02 2009-10-01 Bionanosensor detection device
EP09818504.4A EP2334811B1 (fr) 2008-10-02 2009-10-01 Dispositif de détection par nanocapteur biologique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19498608P 2008-10-02 2008-10-02
US61/194,986 2008-10-02

Publications (4)

Publication Number Publication Date
WO2010039941A2 WO2010039941A2 (fr) 2010-04-08
WO2010039941A3 true WO2010039941A3 (fr) 2010-05-27
WO2010039941A9 WO2010039941A9 (fr) 2010-12-02
WO2010039941A8 WO2010039941A8 (fr) 2014-07-24

Family

ID=42074211

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/059208 Ceased WO2010039941A2 (fr) 2008-10-02 2009-10-01 Dispositif de détection par nanocapteur biologique

Country Status (5)

Country Link
US (1) US9919922B2 (fr)
EP (1) EP2334811B1 (fr)
AU (1) AU2009298497B2 (fr)
CA (1) CA2739370A1 (fr)
WO (1) WO2010039941A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11713330B2 (en) 2019-04-16 2023-08-01 Helixbind, Inc. Methods and devices for ultrasensitive direct detection of microorganisms
CA3208356A1 (fr) * 2021-01-20 2022-07-28 12-15 Molecular Diagnostics, Inc. Capteur auto-desinfectant et procede de fonctionnement
WO2025054393A1 (fr) * 2023-09-08 2025-03-13 12-15 Molecular Diagnostics, Inc. Procédé et dispositif de détection de maladies, d'agents pathogènes et de parasites

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US20070045756A1 (en) * 2002-09-04 2007-03-01 Ying-Lan Chang Nanoelectronic sensor with integral suspended micro-heater
US20070134696A1 (en) * 2002-11-21 2007-06-14 Ming Zheng Dispersion of carbon nanotubes by nucleic acids
US20070158766A1 (en) * 2000-12-11 2007-07-12 President And Fellows Of Harvard College Nanosensors
US20070172829A1 (en) * 2001-12-04 2007-07-26 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting Borrelia burgdorferi
US20070212677A1 (en) * 2004-11-22 2007-09-13 Odyssey Thera, Inc. Identifying off-target effects and hidden phenotypes of drugs in human cells
US20070278111A1 (en) * 2004-09-30 2007-12-06 Salah Boussaad Redox potential mediated, heterogeneous, carbon nanotube biosensing
US20080004368A1 (en) * 2004-11-18 2008-01-03 Shanfeng Wang Block Copolymers of Polycarpolactone and Poly (Propylene Funarate)
US20080044651A1 (en) * 2004-06-02 2008-02-21 Mysticmd Inc. Coatings Comprising Carbon Nanotubes
US20080131880A1 (en) * 2006-11-22 2008-06-05 Bortolin Laura T PNA-DNA oligomers and methods of use thereof

Family Cites Families (18)

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US6376177B1 (en) 1999-10-06 2002-04-23 Virtual Pro, Inc. Apparatus and method for the analysis of nucleic acids hybridization on high density NA chips
WO2001079529A1 (fr) 2000-04-17 2001-10-25 Purdue Research Foundation Biocapteur et procede associe
US6958216B2 (en) * 2001-01-10 2005-10-25 The Trustees Of Boston College DNA-bridged carbon nanotube arrays
US7052854B2 (en) 2001-05-23 2006-05-30 University Of Florida Research Foundation, Inc. Application of nanotechnology and sensor technologies for ex-vivo diagnostics
US6824974B2 (en) 2001-06-11 2004-11-30 Genorx, Inc. Electronic detection of biological molecules using thin layers
US7318908B1 (en) * 2001-11-01 2008-01-15 The Board Of Trustees Of The Leland Stanford Junior University Integrated nanotube sensor
AU2002951274A0 (en) 2002-08-30 2002-09-19 Commonwealth Scientific And Industrial Research Organisation Methods for the chemical and physical modification of nanotubes, methods for linking the nanotubes, methods for the directed positioning of nanotubes, and uses thereof
US20030134410A1 (en) * 2002-11-14 2003-07-17 Silva Robin M. Compositions and methods for performing biological reactions
US7163659B2 (en) * 2002-12-03 2007-01-16 Hewlett-Packard Development Company, L.P. Free-standing nanowire sensor and method for detecting an analyte in a fluid
EP1781771A2 (fr) * 2004-08-24 2007-05-09 Nanomix, Inc. Dispositifs de detection a nanotubes, destines a la detection de sequences d'adn
WO2006052891A1 (fr) * 2004-11-09 2006-05-18 The Regents Of The University Of California Identification d'analytes au moyen de dispositifs electroniques
JP2008525822A (ja) * 2004-12-28 2008-07-17 ナノミックス・インコーポレーテッド ポリヌクレオチド配列のｄｎａ検出／認識用ナノ電子装置
JP2009540798A (ja) * 2006-03-15 2009-11-26 プレジデント アンド フェロウズ オブ ハーバード カレッジ ナノバイオエレクトロニクス
JP2009540333A (ja) 2006-06-12 2009-11-19 プレジデント アンド フェロウズ オブ ハーバード カレッジ ナノセンサーおよび関連技術
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EP2049436B1 (fr) 2006-08-11 2012-10-17 Agency for Science, Technology and Research Capteur à nanofil, réseau de capteurs à nanofils et leur méthode de fabrication
JP4953860B2 (ja) 2007-03-09 2012-06-13 独立行政法人科学技術振興機構 イオン性カードラン誘導体を用いるナノ構造体
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Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070158766A1 (en) * 2000-12-11 2007-07-12 President And Fellows Of Harvard College Nanosensors
US20070172829A1 (en) * 2001-12-04 2007-07-26 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting Borrelia burgdorferi
US20070045756A1 (en) * 2002-09-04 2007-03-01 Ying-Lan Chang Nanoelectronic sensor with integral suspended micro-heater
US20070134696A1 (en) * 2002-11-21 2007-06-14 Ming Zheng Dispersion of carbon nanotubes by nucleic acids
US20080044651A1 (en) * 2004-06-02 2008-02-21 Mysticmd Inc. Coatings Comprising Carbon Nanotubes
US20070278111A1 (en) * 2004-09-30 2007-12-06 Salah Boussaad Redox potential mediated, heterogeneous, carbon nanotube biosensing
US20080004368A1 (en) * 2004-11-18 2008-01-03 Shanfeng Wang Block Copolymers of Polycarpolactone and Poly (Propylene Funarate)
US20070212677A1 (en) * 2004-11-22 2007-09-13 Odyssey Thera, Inc. Identifying off-target effects and hidden phenotypes of drugs in human cells
US20080131880A1 (en) * 2006-11-22 2008-06-05 Bortolin Laura T PNA-DNA oligomers and methods of use thereof

Non-Patent Citations (1)

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Also Published As

Publication number Publication date
AU2009298497A1 (en) 2010-04-08
WO2010039941A2 (fr) 2010-04-08
EP2334811A2 (fr) 2011-06-22
AU2009298497B2 (en) 2013-12-19
WO2010039941A8 (fr) 2014-07-24
EP2334811B1 (fr) 2015-06-03
CA2739370A1 (fr) 2010-04-08
US9919922B2 (en) 2018-03-20
EP2334811A4 (fr) 2012-05-16
US20120178639A1 (en) 2012-07-12
WO2010039941A9 (fr) 2010-12-02

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