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WO2010028019A3 - Direct reprogramming of somatic cells using non-integrating vectors - Google Patents

Direct reprogramming of somatic cells using non-integrating vectors Download PDF

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Publication number
WO2010028019A3
WO2010028019A3 PCT/US2009/055698 US2009055698W WO2010028019A3 WO 2010028019 A3 WO2010028019 A3 WO 2010028019A3 US 2009055698 W US2009055698 W US 2009055698W WO 2010028019 A3 WO2010028019 A3 WO 2010028019A3
Authority
WO
WIPO (PCT)
Prior art keywords
somatic cells
integrating vectors
pluripotent stem
direct reprogramming
stem cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/055698
Other languages
French (fr)
Other versions
WO2010028019A2 (en
Inventor
Konrad Hochedlinger
Matthias Stadtfeld
Nimet Maherali
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Harvard University
Original Assignee
General Hospital Corp
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Hospital Corp, Harvard University filed Critical General Hospital Corp
Publication of WO2010028019A2 publication Critical patent/WO2010028019A2/en
Publication of WO2010028019A3 publication Critical patent/WO2010028019A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Provided herein are methods for producing induced pluripotent stem cells with the use of a non-integrating vector. Reprogramming of cells using a non-integrating vector (e.g., adenoviral vector) permits the generation of induced pluripotent stem cells that do not contain leftover viral genes that can be reactivated in the cell. This type of transient reprogramming is further contemplated for production of induced pluripotent stem cells for delivery to an individual.
PCT/US2009/055698 2008-09-03 2009-09-02 Direct reprogramming of somatic cells using non-integrating vectors Ceased WO2010028019A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9385008P 2008-09-03 2008-09-03
US61/093,850 2008-09-03

Publications (2)

Publication Number Publication Date
WO2010028019A2 WO2010028019A2 (en) 2010-03-11
WO2010028019A3 true WO2010028019A3 (en) 2010-06-24

Family

ID=41797822

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/055698 Ceased WO2010028019A2 (en) 2008-09-03 2009-09-02 Direct reprogramming of somatic cells using non-integrating vectors

Country Status (1)

Country Link
WO (1) WO2010028019A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101516833B1 (en) 2007-03-23 2015-05-07 위스콘신 얼럼나이 리서어치 화운데이션 Reprogramming somatic cells
JP2011522540A (en) * 2008-06-04 2011-08-04 セルラー ダイナミクス インターナショナル， インコーポレイテッド Method for production of iPS cells using a non-viral approach
CA2734128A1 (en) 2008-08-12 2010-02-18 Cellular Dynamics International, Inc. Methods for the production of ips cells
US9228204B2 (en) 2011-02-14 2016-01-05 University Of Utah Research Foundation Constructs for making induced pluripotent stem cells
PL2852671T3 (en) 2012-05-21 2019-06-28 The Regents Of The University Of California MANUFACTURE OF HUMAN IPS CELLS USING SYNTHETIC SELFPLICATING RNA

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100011053A (en) * 2008-07-24 2010-02-03 차의과학대학교 산학협력단 Method for preparing induced pluripotent stem cells using herpes simplex amplicon viruses containing reprogramming-inducing genes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100011053A (en) * 2008-07-24 2010-02-03 차의과학대학교 산학협력단 Method for preparing induced pluripotent stem cells using herpes simplex amplicon viruses containing reprogramming-inducing genes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AOI, T. ET AL.: "Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells.", SCIENCE, vol. 321, 1 August 2008 (2008-08-01), pages 699 - 702 *
HIRSCH, T. ET AL.: "Adenoviral Gene Delivery to Primary Human Cutaneous Cells and Burn Wounds.", MOL. MED., vol. 12, no. 9-10, 2006, pages 199 - 207 *
OKITA, K. ET AL.: "Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors.", SCIENCE, vol. 322, October 2008 (2008-10-01), pages 949 - 953 *
STADTFELD, M. ET AL.: "Defining Molecular Cornerstones during Fibrobalst to iPS Cell Reprogramming in Mouse.", CELL STEM CELL., vol. 2, March 2008 (2008-03-01), pages 230 - 240 *
STADTFELD, M. ET AL.: "Induced Pluripotent Stem Cells Generation Withoug Viral Integration.", SCIENCE, vol. 322, 25 September 2008 (2008-09-25), pages 945 - 949 *

Also Published As

Publication number Publication date
WO2010028019A2 (en) 2010-03-11

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