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WO2010006166A3 - Bead manipulation techniques - Google Patents

Bead manipulation techniques Download PDF

Info

Publication number
WO2010006166A3
WO2010006166A3 PCT/US2009/050101 US2009050101W WO2010006166A3 WO 2010006166 A3 WO2010006166 A3 WO 2010006166A3 US 2009050101 W US2009050101 W US 2009050101W WO 2010006166 A3 WO2010006166 A3 WO 2010006166A3
Authority
WO
WIPO (PCT)
Prior art keywords
droplet
magnetically responsive
responsive beads
beads
redistributing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/050101
Other languages
French (fr)
Other versions
WO2010006166A2 (en
Inventor
Vamsee Pamula
Prasanna Thwar
Arjun Sudarsan
Ramakrishna Sista
Michael Sullivan
Scott Norton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advanced Liquid Logic Inc
Original Assignee
Advanced Liquid Logic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic Inc filed Critical Advanced Liquid Logic Inc
Publication of WO2010006166A2 publication Critical patent/WO2010006166A2/en
Publication of WO2010006166A3 publication Critical patent/WO2010006166A3/en
Priority to US12/985,409 priority Critical patent/US8637317B2/en
Anticipated expiration legal-status Critical
Priority to US14/163,442 priority patent/US20140193807A1/en
Priority to US14/491,231 priority patent/US9050606B2/en
Priority to US14/697,058 priority patent/US9205433B2/en
Priority to US14/928,096 priority patent/US9358551B2/en
Priority to US15/165,883 priority patent/US20160271623A1/en
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/028—Non-uniform field separators using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode -mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
PCT/US2009/050101 2006-04-13 2009-07-09 Bead manipulation techniques Ceased WO2010006166A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US12/985,409 US8637317B2 (en) 2006-04-18 2011-01-06 Method of washing beads
US14/163,442 US20140193807A1 (en) 2006-04-18 2014-01-24 Bead manipulation techniques
US14/491,231 US9050606B2 (en) 2006-04-13 2014-09-19 Bead manipulation techniques
US14/697,058 US9205433B2 (en) 2006-04-13 2015-04-27 Bead manipulation techniques
US14/928,096 US9358551B2 (en) 2006-04-13 2015-10-30 Bead manipulation techniques
US15/165,883 US20160271623A1 (en) 2006-04-13 2016-05-26 Bead Manipulation Techniques

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US7934608P 2008-07-09 2008-07-09
US61/079,346 2008-07-09
US8073108P 2008-07-15 2008-07-15
US61/080,731 2008-07-15
US10330208P 2008-10-07 2008-10-07
US61/103,302 2008-10-07
US10899708P 2008-10-28 2008-10-28
US61/108,997 2008-10-28
US12279108P 2008-12-16 2008-12-16
US61/122,791 2008-12-16
US14980809P 2009-02-04 2009-02-04
US61/149,808 2009-02-04

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US11/639,531 Continuation US8613889B2 (en) 2006-04-13 2006-12-15 Droplet-based washing
US12/985,409 Continuation US8637317B2 (en) 2006-04-13 2011-01-06 Method of washing beads

Publications (2)

Publication Number Publication Date
WO2010006166A2 WO2010006166A2 (en) 2010-01-14
WO2010006166A3 true WO2010006166A3 (en) 2010-04-29

Family

ID=41507733

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/050101 Ceased WO2010006166A2 (en) 2006-04-13 2009-07-09 Bead manipulation techniques

Country Status (1)

Country Link
WO (1) WO2010006166A2 (en)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1859330T3 (en) 2005-01-28 2012-10-15 Univ Duke DEVICES AND METHODS OF HANDLING SMALL DROPS ON A PRINTED CIRCUIT CARD
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
EP2570812B1 (en) 2007-02-09 2018-07-18 Advanced Liquid Logic, Inc. Method of assembling a droplet actuator assembly
EP2109774B1 (en) 2007-02-15 2018-07-04 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US9631244B2 (en) 2007-10-17 2017-04-25 Advanced Liquid Logic, Inc. Reagent storage on a droplet actuator
CA2709928A1 (en) 2007-12-23 2009-07-09 Advanced Liquid Logic, Inc. Droplet actuator configurations and methods of conducting droplet operations
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
EP2286228B1 (en) 2008-05-16 2019-04-03 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
US9248450B2 (en) 2010-03-30 2016-02-02 Advanced Liquid Logic, Inc. Droplet operations platform
WO2011137533A1 (en) 2010-05-05 2011-11-10 The Governing Council Of The University Of Toronto Method of processing dried samples using digital microfluidic device
EP2588322B1 (en) 2010-06-30 2015-06-17 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
TWI419738B (en) * 2011-03-18 2013-12-21 Ind Tech Res Inst Particle transporter
CA2833897C (en) 2011-05-09 2020-05-19 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
EP2707724A4 (en) 2011-05-10 2015-01-21 Advanced Liquid Logic Inc Enzyme concentration and assays
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
US20130109596A1 (en) 2011-09-26 2013-05-02 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
BR112014032727B1 (en) 2012-06-27 2021-12-14 Illumina France METHOD AND SYSTEM FOR PERFORMING DROP OPERATIONS ON A DROPOLE ON A DROP ACTUATOR TO REDUCE BUBBLE FORMATION
US9863913B2 (en) 2012-10-15 2018-01-09 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
FI127992B (en) 2014-08-29 2019-07-15 Svanbaeck Sami Procedure and systems for determining dissolution properties of a substance
CN115927293A (en) * 2014-12-09 2023-04-07 生命技术公司 Efficient small volume nucleic acid synthesis
US10695762B2 (en) 2015-06-05 2020-06-30 Miroculus Inc. Evaporation management in digital microfluidic devices
EP3303547A4 (en) 2015-06-05 2018-12-19 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
WO2018039281A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
CA3049416A1 (en) 2016-12-28 2018-07-05 Miroculus Inc. Digital microfluidic devices and methods
WO2018187476A1 (en) 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
EP3658908B1 (en) 2017-07-24 2025-11-12 Integra Biosciences AG Digital microfluidics systems and methods with integrated plasma collection device
CN115582155B (en) 2017-09-01 2025-08-26 因特格拉生物科学股份公司 Digital microfluidic device and method of use thereof
EP3796999A4 (en) 2018-05-23 2022-03-09 Miroculus Inc. CONTROL OF EVAPORATION IN DIGITAL MICROFLUIDICS
EP3917670A4 (en) 2019-01-31 2022-11-02 Miroculus Inc. NON-POLLUTING COMPOSITIONS AND METHODS FOR MANIPULATING AND PROCESSING ENCAPSULATED DROPLETS
CN119158636A (en) 2019-04-08 2024-12-20 米罗库鲁斯公司 Multi-cassette digital microfluidic device and method of use
WO2021016614A1 (en) 2019-07-25 2021-01-28 Miroculus Inc. Digital microfluidics devices and methods of use thereof
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
US20240253040A1 (en) * 2023-01-30 2024-08-01 Idexx Laboratories, Inc. Methods, Systems, and Device for Electrowetting Bar-Coded Magnetic Beads

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050056569A1 (en) * 2003-07-29 2005-03-17 National Tsing Hua University Electrowetting electrode device with electromagnetic field for actuation of magnetic-bead biochemical detection system
EP1844852A1 (en) * 2006-04-13 2007-10-17 Samsung Electronics Co., Ltd. Apparatus and method for printing biomolecular droplet on substrate
US20070243634A1 (en) * 2006-04-18 2007-10-18 Pamula Vamsee K Droplet-based surface modification and washing
US20070275415A1 (en) * 2006-04-18 2007-11-29 Vijay Srinivasan Droplet-based affinity assays

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050056569A1 (en) * 2003-07-29 2005-03-17 National Tsing Hua University Electrowetting electrode device with electromagnetic field for actuation of magnetic-bead biochemical detection system
EP1844852A1 (en) * 2006-04-13 2007-10-17 Samsung Electronics Co., Ltd. Apparatus and method for printing biomolecular droplet on substrate
US20070243634A1 (en) * 2006-04-18 2007-10-18 Pamula Vamsee K Droplet-based surface modification and washing
US20070275415A1 (en) * 2006-04-18 2007-11-29 Vijay Srinivasan Droplet-based affinity assays

Also Published As

Publication number Publication date
WO2010006166A2 (en) 2010-01-14

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