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WO2010081335A1 - Procédé de production de biocarburant à partir de canne à sucre - Google Patents

Procédé de production de biocarburant à partir de canne à sucre Download PDF

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Publication number
WO2010081335A1
WO2010081335A1 PCT/CN2009/074298 CN2009074298W WO2010081335A1 WO 2010081335 A1 WO2010081335 A1 WO 2010081335A1 CN 2009074298 W CN2009074298 W CN 2009074298W WO 2010081335 A1 WO2010081335 A1 WO 2010081335A1
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originated
carbon source
sugarcane
microorganism
metabolite
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Qingyu Wu
Yun Cheng
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Tsinghua University
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Tsinghua University
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    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock

Definitions

  • the present invention refers to renewable energy.
  • the present invention refers to biofuel production, and more particularly biodiesel production.
  • Biofuel can be broadly defined as solid, liquid or gas fuel derived from biomass, in which liquid biofuel is the most common form for transportation use.
  • the growths of automotive transport and declining petroleum supply have strongly promoted the commercialization of liquid biofuel.
  • biofuel was made from food-based sources, such as bio-alcohols (ethanol, propanol and butanol etc.) from fermentation of corn starches (Smith AM. Prospects for increasing starch and sucrose yields for bioethanol production. Plant J. 2008, 54 : 546-58) and biodiesel from oil plants and animal fats (M. Canakci and H. Sanli. Biodiesel production from various feedstocks and their effects on the fuel properties. J Ind Microbiol Biotechnol.
  • Microorganism-originated biofuels such as microbial-, yeast- and especially algae-originated fuel
  • microorganism-originated fuel has advantages in avoiding threatening food supplies and biodiversity.
  • the recent decade witnessed a surge of interest in microorganism-originated oil, lipid and/or fatty acids, especially algal oil, as a promising supplement of oil source for biodiesel production (Meng X et al. Biodiesel production from oleaginous microorganisms. Renew Energy. 2009, 34 : 1 -5).
  • microorganism-originated fuel hasn't been widely accepted by energy market because of the technical and economic obstacles exist in their commercialization.
  • the cost of cultivating microorganism is still high, and sometimes it is hard to extract or collect interest components from microorganism for subsequent process of fuel preparation.
  • Some species of microorganism including oleaginous yeasts (such as Lipomyces starkeyi, Rhodotorula glutinis and Candida curvata), bacillus (such as Rhodococcus opacus and Acinetobacter calcoaceticus), fungi (such as Mortierella isabellina and Mortierella vinacea) and algae (such as Schizochytrium sp. , Nitzschia sp. and Botryococcus braunii) have been reported to accumulate metabolite (eg. lipids, oil, methyl-esters or soaps) which can be used as sole carbon and energy sources.
  • metabolite eg. lipids, oil, methyl-esters or soaps
  • triglyceride and fatty acids are usually the dominating components of algal oil, from which biodiesel can be prepared by transesterification, yielding monoalkyl esters of fatty acids and alcohols.
  • microorganism can grow with inorganic carbon source and sunlight as energy supply.
  • biomass productivity and oil content of photoautotrophic cultures are extremely low, the photoautotrophic technology mainly suffers from limited supply of light and lower energy conversion efficiencies.
  • microorganism during photoautotrophic cultivation can hardly provide enough useful components that can be prepared into biofuel.
  • the present inventor convincedly discovered that some species of microorganism (such as alga) accumulate very high proportion of oil when specific organic carbon source (such as glucose) is provided to the microorganism. The most important thing is that the accumulated oil can be prepared into high quality biofuel (such as biodiesel). Therefore, the described cultivation strategy allows microorganism to accumulate much higher proportion of oil within less time and the scale-up is much easier.
  • the present invention refers to a method of producing biofuel from microorganism-originated metabolite by using sugarcane as feedstock.
  • the present invention provides method for producing biofuel from microorganism-originated oil, lipid and/or fatty acids by using sugarcane as feedstock.
  • the present invention provides method for producing biodiesel from alga-originated oil, lipid and/or fatty acids by using sugarcane as feedstock.
  • the present invention provides method of producing biofuel, comprising: (a) providing fermentable carbon source of sugarcane;
  • the present invention provides method of producing biodiesel, comprising:
  • the subject invention is particularly applicable to biofuel production, especially biodiesel production.
  • the present invention provided a preferred embodiment of producing biodiesel from algal oil by transesterification using sugarcane-originated carbon source as feedstock.
  • sugarcane-originated carbon source provided in the present invention: bacteria, fungi, archaea, and protists, microscopic plants, alga and microscopic animals.
  • microorganism-originated metabolite can be produced from sugarcane-originated carbon source provided in the present invention: alcohols, fatty acids, oils, lipids, hydrocarbons and the derivatives thereof.
  • the following non-limiting list of techniques can be applied in preparing biofuel from microorganism-originated metabolite: extraction, fractionation, distillation, addition reaction, neutralization, hydrogenation, dehydrogenization, oxidation, reduction, substitution, esterification, transesterification and hydrolysis.
  • Figure 1 shows the comparison of glucose and sugarcane juice-originated carbon source for microalgal heterotrophic cultivation.
  • Figure I Cell density (- ⁇ "-) and residual sugar (TMOTM) in the medium with
  • FIG. 1 shows the comparison of glucose and bagasse-originated carbon source for microalgal heterotrophic cultivation.
  • Figure 2a Cell density ( ⁇ ) and residual sugar (•) in the medium with 1 1 g I " 1 glucose.
  • Figure 2b Cell density ( ⁇ ) and residual sugar (•) in the medium with 11 g I " 1 bagasse-originated carbon source.
  • Figure 3 shows heterotrophic fermentation in 5-1 bioreactor using glucose (a) and juice-originated carbon source (b) as feedstock, respectively.
  • Figure 4 shows microalgal heterotrophic cultivation in juice-originated carbon source media supplemented with and without other nutritional elements.
  • Figure 5 shows microalgal heterotrophic cultivation in non-sterilized medium containing 30 g I " 1 juice-originated carbon source. Cell density (•); residual sugar (D) .
  • Figure 6 is a schematic description that shows the progress of producing biofuel from microorgnism-originated metabolite by using carbon source of sugarcane as feedstock, comprising: (a) providing fermentable carbon source of sugarcane; (b) cultivating microorganism in medium containing the carbon source described in (a); (c) extracting or collecting interest microorganism-originated metabolite from microorganism; (d) preparing biofuel from microorganism- originated metabolite.
  • the present invention provides method of producing biofuel from microorganism-originated metabolite by using sugarcane as feedstock.
  • the present invention provides method for producing biodiesel from alga-originated oil, lipid and/or fatty acids by using sugarcane as feedstock.
  • the present invention provides method of producing biofuel, comprising:
  • the present invention provides method of producing biodiesel, comprising:
  • biofuel refers to solid, liquid or gaseous fuel obtained from renewable biological material. Biofuel is different from fossil fuels, which are derived from long dead biological material. Also, various plants and plant-derived materials are used for biofuel manufacturing.
  • biodiesel refers to non-petroleum-based diesel fuel consisting of alkyl (eg. methyl, propyl or ethyl) esters. Biodiesel is made by chemically-reacting lipids, typically vegetable oil or animal fat, and alcohol. It can be used alone or blended with conventional petrodiesel in unmodified diesel-engine vehicles (Specification for Biodiesel (B l OO)-ASTM D6751 ).
  • microorganism refers to an organism that is microscopic. Microorganisms are very diverse, they include bacteria, fungi, archaea, and protists, microscopic plants, algae and microscopic animals. In this invention, microorganism is cultivated in medium containing carbon source of sugarcane. Therefore, microorganism refers to microorganism that has the ability of using organic substrates to maintain life cycle and to generate interest metabolite that can be processed into biofuel or directly served as biofuel, unless otherwise noted.
  • lipid is broadly defined as any fat-soluble molecule, such as fats, oils, waxes, cholesterol, sterols, fat-soluble vitamins, monoglycerides, diglycerides, triglycerides, phospholipids and others.
  • oil includes compound classes with otherwise unrelated chemical structures, properties and uses, including vegetable oils, petrochemical oils, volatile essential oils and microorganism-originated oils. Oil is a non-polar substance.
  • triglyceride also known as triacylglycerol, TAG or triacylglyceride
  • TAG triacylglyceride
  • sucgarcane juice refers to the liquid extracted from the crushed stalks of sugarcane.
  • bagasse refers to the dry, fibrous residue remaining after the extraction of juice from the crushed stalks of sugarcane.
  • transesterification refers to a process of exchanging the alcohol group of an ester compound with another alcohol.
  • heterotrophic refers to an organism or condition that uses organic substrates to get its chemical energy for its life cycle.
  • fermentable carbon source refers to the organic carbon source that can be directly or indirectly utilized by heterotrophic microorganism.
  • reducing sugar refers to any sugar that, in basic solution, forms some aldehyde or ketone. This allows the sugar to act as a reducing agent and react with, for example, 3,5-dinitrosalicylic acid (DNS), Benedict's reagent and Benedicting's solution. Reducing sugars include glucose, fructose, lactose, arabinose, maltose and glyceraldehyde. Significantly, sucrose and cellulose are not reducing sugars. According to one aspect of the present invention, the present invention provides method of providing fermentable carbon source to microorganism.
  • Carbon source is a nutrient that provides carbon skeletons needed for synthesis of new organic molecules, which can be classified into organic and inorganic carbon sources.
  • Carbon dioxide is known as inorganic carbon source.
  • the most common organic carbon source for heterotrophic microorganism includes, but not limited to, pentose, hexose, acetate, starch, sucrose, etc.
  • the carbon sources in sugarcane stalks are mainly juice-originated carbon source (sucrose, fructose and glucose etc.) and bagasse-originated carbon source (cellulose etc.), which are ideal for preparing fermentation media.
  • organic carbon source such as but not limited to bacteria, fungi, archaea, and protists, microscopic plants, algae and microscopic animals.
  • the recombinant or mutant species are also included in the spirit of the present invention.
  • alga was administrated with the described sugarcane-originated carbon source.
  • Chlorella protothecoides was chosen as the subject administrated with the described sugarcane-originated carbon source, because C. protothecoides has the ability of accumulating oil at high content whenever organic carbon source is provided.
  • the known organic carbon source of C. protothecoides is hexose.
  • sugarcane was processed before fermentation.
  • juice and bagasse were obtained and processed respectively.
  • the method used to separate juice from bagasse can be any device, method or technique that are known to those skilled in the field, includes but not limited to centrifugation, crushing, milling, grinding.
  • the sugarcane stalks was processed by juice extractor device equipped with roller mill apparatus, which is available in market. And the extracted sugarcane juice was collected and filtered through a screen filter.
  • the dominating carbon source in sugarcane juice is sucrose.
  • Sucrose is a disaccharide of glucose and fructose.
  • hydrolysis can be catalyzed by acid, base and enzyme.
  • sugarcane juice was supplemented with fructofuranosidase to achieve the hydrolysis of sucrose into glucose and fructose.
  • the dominating carbon source in bagasse is cellulose.
  • Cellulose is a polysaccharide consisting of a linear chain of several hundred to over ten thousand (1 ⁇ 4) linked D-glucose units.
  • hydrolysis can be catalyzed by acid, base and enzyme.
  • bagasse was supplemented with cellulase to achieve the hydrolysis of cellulose into glucose.
  • the preferred embodiments should not be considered as limitation, some other species may utilize the sugarcane-originated carbon source without or with different processing.
  • the present invention provides method of cultivating microorganism in medium containing the sugarcane originated-carbon source provided according to the present invention.
  • the sugarcane originated-carbon source can be provided to microorganism in any form, by any pathway or strategy, at any amount, which depends on the specific needs of specific microorganism.
  • the sugarcane originated-carbon source can be provided once at a time or by fed-batch.
  • the sugarcane originated-carbon source can be provided in the form of liquid media or solid media.
  • the sugarcane originated-carbon source can be provided at a concentration allowing microorganism to accumulate metabolite suitable for biofuel preparation.
  • the media containing sugarcane originated-carbon source can be sterilized or non-sterilized.
  • the media containing sugarcane originated-carbon source can be supplemented with or without other components, which depends on the needs of specific microorganism.
  • Various devices and conditions for cultivating microorganism are well known and readily used by those skilled in the art and include, but not limited to, bioreactor, flask, incubator, pH, dissolved oxygen, temperature, stirrer, inoculation amount, shaking speed, ventilation or light etc.
  • alga was cultivated in sterilized liquid medium containing sugarcane (juice or bagasse) originated-carbon source.
  • alga was cultivated in non-sterilized liquid medium containing sugarcane originated-carbon source.
  • nitrogen source such yeast extract
  • ion source such as phosphate, magnesium salt, ferric salt
  • vitamin such as VB i
  • trace element such as VB i
  • alga was cultivated in flask with shaken, and in another preferred embodiment, alga was cultivated in bioreactor. In a preferred embodiment, alga was cultivated in media containing sugarcane originated-carbon source with an initial concentration of 1 -60 g I "1 , preferably at 5-50 g I "1 , more preferably at 10-30 g I "1 .
  • the present invention provides method of extracting or collecting interest microorganism-originated metabolite by using sugarcane-originated carbon source as feedstock.
  • the following non-limiting list of microorganism-originated metabolite can be produced from sugarcane-originated carbon source provided in the present invention: alcohols, fatty acids, oils, lipids, hydrocarbons and the derivatives thereof.
  • the devices and methods for extracting or collecting interest metabolite depend on the character of interest metabolite, the condition of subject microorganism and the needs of subsequent process. Various devices and methods for extracting or collecting interest microorganism-originated metabolite are well known to those skilled in the art.
  • the interest microorganism-originated metabolite is algal oil, in which triglyceride and fatty acids are the dominating components.
  • algal oil can be easily extracted by organic reagent such as but not limited to acetone, n-hexane, chloroform, methane, acetonitrile etc.
  • algal oil was extracted from the algal cells by Soxhlet apparatus with n-hexane as solvent. After removing n-hexane by a rotary evaporator, oil was obtained.
  • the present invention provides method of preparing biofuel from microorganism-originated metabolite obtained according to the present invention.
  • the following non-limiting list of techniques can be applied in preparing biofuel from microorganism-originated metabolite: extraction, fractionation, distillation, addition reaction, neutralization, hydrogenation, dehydrogenization, oxidation, reduction, substitution, esterification, transesterification and hydrolysis.
  • the method of preparing biofuel from microorganism-originated metabolite depends on the character of interest metabolite. In a prepared embodiment, the inventor unexpectedly found that specific metabolite, preferably oil, was accumulated in algal cells when the described sugarcane-originated carbon source was administrated to alga.
  • the inventor also unexpectedly found that the specific metabolite, preferably algal oil, was extremely suitable for biodiesel preparation.
  • the extracted algal oil was processed into biodiesel by transesterification. The process involves reacting oils catalytically with short-chain aliphatic alcohols (typically methanol or ethanol). These reactions are often catalyzed by the addition of acid, base or enzyme.
  • the said algal oil reacted with methanol by lipase catalysis.
  • Example 1 Microorganisms, maintenance and inoculum
  • Chlorella protothecoides was cultivated in basic medium supplemented with 10 g l ⁇ glucose and 3 g I "1 yeast extract (YE).
  • Basic medium contains: 0.7 g I "1 KH 2 PO 4 , 0.3 g T 1 K 2 HPO 4 , 0.3 g I "1 MgSO 4 » 7H 2 O, 0.3 mg I "1 FeSO 4 » 7H 2 O and 0.01 mg I "1 VBi .
  • Heterotrophic cells in exponential phase were used to inoculate fresh media.
  • sugarcane juice was extracted from the sugarcane stalks by juice extractor equipped with roller mill apparatus; the extracted sugarcane juice was collected and filtered through a screen filter; the extracted juice was supplemented with fructofuranosidase (Valisase R Ivertase ANL, 15000 Valley Summer Unit g " 1 ) and hydrolyzed according to the instruction provided by manufacturer. The concentration of reducing sugar (hydrolysate) was monitored at regular intervals by dinitrosalicyclic acid (DNS) method to determine the complete digestion.
  • DFS dinitrosalicyclic acid
  • the bagasse was collected after the extraction of juice from the crushed stalks of sugarcane. The bagasse was washed with water for 3 times to remove the residual juice, and then air-dried to constant weight. The bagasse was processed into dry powder by mill before later use. 50 g of the bagasse powder was added into 1000 ml of 50 mM citrate buffer (pH 4.8), and then supplemented with 6.5 ml cellulase (DENICELL 101L, India) to make a reaction mixture. The mixture was incubated at 50 0 C, 140 rpm for 24-36 hours, and then the mixture was inactivated at 100 0 C for 5 min. The supernate was collected by centrifugation (100Og, 10 min) for cultivating alga. The concentration of reducing sugar in supernate was measured by DNS method.
  • Example 5 Cultivation in shake flask with carbon source originated from bagasse as feedstock
  • basic medium supplemented with H g l ⁇ glucose and 3 g I "1 YE were respectively used as positive control to investigate the cell growth and oil accumulation of algal cells in basic medium supplemented with 11 g I "1 bagasse-originated carbon source and 3 g I "1 YE ( Figure 2 b). All media and cultivation apparatus were sterilized with steam at 112°C, 0.12 Mpa for 30 min. C. protothecoides in exponential phase was inoculated into media at equivalent initial cell density.
  • Heterotrophic cultivation was carried out in 500 ml flasks containing 200 ml medium at 28 ⁇ 1 °C with continuous shaking (220 rpm). Samples were taken at regular intervals to determine the cell density and sugar concentration, curves of cell growth and sugar consumption were recorded.
  • Algal heterotrophic fermentation in bioreactors instead of cultivation in shake flask, is commonly used in practical production. Therefore algal fermentations in 5-1 bioreactors (Minifors, INFORS AG CH-4103, Bottmingen, Switzerland) containing 3-1 medium were conducted with 3 g I "1 YE and 30 g I "1 glucose ( Figure 3 a) or juice-originated carbon source ( Figure 3 b) as the starting condition. Carbon source and YE were batch-fed whenever the carbon source was exhausted.
  • Example 7 Cultivation in sugarcane juice-originated carbon source media supplemented with and without other nutritional elements
  • the sugarcane juice prepared in example 2 was adjusted to 20 g I "1 (the concentration of reducing sugar) and served as medium (a).
  • Medium (b) was prepared by adding 3 g I "1 YE, 0.7 g I "1 KH 2 PO 4 , 0.3 g 1 "1 K 2 HPO 4 , 0.3 g I "1 MgSO 4 «7H 2 O, 0.3 mg I "1 FeSO 4 «7H 2 O and 0.01 mg I "1 VB 1 to medium (a). All media and cultivation apparatus were sterilized with steam at 112°C, 0.12 Mpa for 30 min. C.
  • Example 8 Cultivation in non-sterilized medium containing sugarcane-originated carbon source
  • the basic medium described in example 1 was supplemented with 30 g I "1 sugarcane juice-originated carbon source prepared according to the method in example 2. All media and cultivation apparatus were not sterilized before using.
  • C. protothecoides in exponential phase was inoculated into media at a relative higher inoculation concentration (about 4 g T 1 ).
  • Heterotrophic cultivation was carried out in 500 ml flasks containing 200 ml medium at 28 ⁇ 1 °C with continuous shaking (220 rpm). Samples were taken at regular intervals to determine the cell density and sugar concentration, curves of cell growth and sugar consumption were recorded ( Figure 5).
  • Biodiesel preparation from algal oil Alga-based biodiesel was prepared by transesterification of algal oil by using sugarcane-originated carbon source.
  • the lipase catalyzed transesterification was performed in shaking flasks and heated to the reaction temperature on a constant temperature shaker, with the rotation rate of 180 rpm.
  • the reaction conditions were 30% immobilized lipase (w/w, 12,000 U), 10% water content (w/w) based on lipids quantity and 3 : 1 molar ratio of methanol to oil, at the temperature of 38 0 C and the pH value of 7.0
  • biodiesel such as density, viscosity, flash point, cold filter plugging point, solidifying point and heating value were also measured.
  • juice-originated carbon source can be used as carbon source appropriate to algal utilization. It is showed that the biomass, oil content and conversion ratio obtained in media containing juice-originated carbon source reached equivalent levels to those of glucose media (Table 1 ). Table 1. The effects of different carbon source feedings on heterotrophic cultivation (in 500 ml flasks containing 200 ml media).
  • Feedings sugar (g) yield (g) (g) Biomass/Sugar Qj l/ Sugar
  • the present inventor proved that media containing sugarcane-originated carbon source can be used directly, and there is no need to add other nutritional elements (example 7). Therefore the cost of cultivation was reduced.
  • cells in sole sugarcane-originated carbon source grew faster and consumed more carbon source during the first two days, but reach a lower level of maximum cell density than that with supplementation, less nitrogen in the source feed may account for the lower level. Nevertheless, the result indicated that sugarcane-originated carbon source can be used directly as feedstock for microalgal cultivation.
  • the main fatty acids methyl esters detected in biodiesel from both juice-originated and bagasse-originated carbon source include 9-Octadecenoic acid methyl ester, 9, 12-Octadecadienoic acid methyl ester and hexadecenoic acid methyl ester. Other minor methyl esters were also detected (Table 4). This result illustrates that the main components of biodiesel from both juice and bagasse feeding are similar.
  • Table 4 The components of biodiesel produced from sugarcane- originated carbon souce feeding.
  • Biodiesel obtained from heterotrophic algal oil by transesterification was characterized by a density of 0.864 kg-L “1 , a higher heating value of 41 MJ-kg "1 and a viscosity of 5.2 ⁇ l O ⁇ 4 Pa-s (at 40 0 C) (Table 5).

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Abstract

La présente invention concerne un procédé permettant de produire du biocarburant à partir d'un métabolite d'un micro-organisme en utilisant de la canne à sucre comme matière première. Dans un mode de réalisation préféré, une algue est cultivée en utilisant le carbone issu de la canne à sucre, ce qui entraîne une accumulation d'huile d'algue dans les cellules d'algue, du biodiesel étant produit à partir de ladite huile d'algue par transestérification.
PCT/CN2009/074298 2009-01-16 2009-09-28 Procédé de production de biocarburant à partir de canne à sucre Ceased WO2010081335A1 (fr)

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CN2009100004641A CN101475823B (zh) 2009-01-16 2009-01-16 以甘蔗作为原料生产生物柴油的方法
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US20220348862A9 (en) * 2019-07-12 2022-11-03 Buckman Laboratories International, Inc. System and method for optimization of the fermentation process
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