WO2009131491A1 - Method for cultivating pearls of specified shape and size - Google Patents
Method for cultivating pearls of specified shape and size Download PDFInfo
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- WO2009131491A1 WO2009131491A1 PCT/RU2009/000162 RU2009000162W WO2009131491A1 WO 2009131491 A1 WO2009131491 A1 WO 2009131491A1 RU 2009000162 W RU2009000162 W RU 2009000162W WO 2009131491 A1 WO2009131491 A1 WO 2009131491A1
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- core
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Definitions
- the invention relates to the production of cultured pearls and can be used to obtain large (from 20 mm) pearls of any shape on freshwater and sea pearls, as well as on mollusks that form the pearlescent layer.
- Modern pearl cultivation is mainly based on three main techniques. Firstly, spherical pearls of all sizes are obtained using the well-known technology of implanting a tissue implant using a core on various types of both sea pearls and freshwater mussels. Secondly, hemispherical pearls with a size of 10-22 mm are obtained using the so-called “blister” technology, again on pearls (oysters) and freshwater mussels. Thirdly, irregularly shaped pearls, or baroque, are obtained on freshwater mollusks using the process of reusable implantations without using a core.
- pearl oysters are used as a mollusk.
- SUBSTITUTE SHEET (RULE 26) A known method of introducing a seed core for growing pearls in the mantle of the mollusks, comprising opening the cusps, piercing the edge tissue of the mantle of the mollusk and introducing the seed core (ed. Certificate. USSR N ° 1701214).
- the piercing is carried out with L-shaped sponges, before piercing, the core is placed between the lips, tweezers are inserted when piercing at an angle to the transverse axis of the leaflet, and then the lips are opened and the mollusk tissues are pushed apart with the formation of a L-shaped through hole tunnel to accommodate the core.
- the tissue under the action of the elastic forces of the muscles of the mollusk tightly fixes the core, preventing its loss.
- the hole in the tissue is sealed with glue and the mollusk is returned to the habitat.
- the disadvantage of this introduction of the kernel is the injury to the mollusk and its subsequent death.
- a core is used in the form of a bead made of plastic resin, which is attached to a thin needle and laid on a pair of disks (internal with a smaller diameter and external with a large diameter) with which the hole in the sink is closed. Due to the needle, the core is fixed on the sink.
- the disadvantage of this method is that the resulting pearls were not large and round, but only hemispherical and small, while a large percentage of death of maternal mollusks was observed.
- the abalone is single-leaf and requires great care and attention than pearl oysters, which are bivalves.
- the closest technical solution to the claimed method is a method of culturing pearls of a given shape and size, including forming a hole in the mollusk shell, introducing the core into the mollusk through the hole obtained, sealing the shell and forming a pearl layer on the core (US Pat. No. 5,347,951).
- the core has a predetermined configuration and consists of the first part, around which mother-of-pearl is formed when the core is inserted into the mollusk through the hole.
- the other part of the core is a cork with a disk, which is firmly connected to the first part, and is equipped with retaining means on the outside of the sink to physically block the hole with a sufficient degree of force.
- the outer surface of the holding means has cuts or an inclined or hyperbolic surface.
- the disadvantage is the impossibility of cultivating large pearls, the large mortality of mollusks due to rejection of a foreign body, many traumatic operations, insufficient sealing of the shell after the introduction of the seed core.
- the level of infection is also relatively high, mainly due to tissue injuries.
- the purpose of the proposed technical solution is the cultivation of large pearls, reducing the mortality of mollusks due to the absence of rejection of a foreign body, the reliability and effectiveness of the method of cultivating pearls.
- This goal is achieved through the use of the proposed method of culturing pearls of a given shape and size, including the formation of a hole in the mollusk shell, introducing the core into the mollusk through the hole obtained, sealing the shell hole and the subsequent formation of the pearl layer on the core, while introducing the core inside
- SUBSTITUTE SHEET (RULE 26) mollusk on the mantle carry out directional compression using the core with the gradual and simultaneous immersion of the core and mantle inside the mollusk at a rate of 0.01 - 2 mm per day until a pearl-forming bag is formed from the mantle, after which the directed compression is stopped.
- the rate of immersion of the core and mantle inside the mollusk ensured the viability of the mollusk and the integrity of the mantle.
- the method is as follows.
- Shellfish are sorted by weight, clean the surface of the sink from contamination. Then, in a mollusk shell, a hole with a diameter larger than the core is usually cut with a diamond drill. A plate with a hole is fixed on the sink with waterproof glue, on which the core is installed with a mechanism that allows for directed compression. The hole and plate are sealed with an underwater epoxy putty or a few drops of superglue. They begin to immerse the core and mantle inside the mollusk due to directional compression. The gradual and simultaneous immersion of the core and mantle inside the mollusk at a rate of 0.01 - 2 mm per day promotes the formation of a pearl-forming bag from the mantle. After the formation of the bag, directional compression is stopped and the formation of a pearl layer begins.
- the viability of a mollusk at the first stage of implantation of a nucleus is assessed visually by its activity, for example, by the activity of the mantle upon irritation, the integrity of the mantle is also determined visually, for example, by endoscopic methods.
- mantle tissue is gradually redistributed and gets used to a foreign body, adapts to it.
- the process of forming a pearl-forming bag from the mantle lasts an average of two weeks, while the mollusk is in an aquarium with sea water. After the operation of introducing the core into the mollusk is completed, the mollusk is again placed in a conventional underwater cage or farm.
- the second stage - the formation of the pearlescent layer - lasts for 3-6 months and depends on the size of the mollusk and the size of the core on which the pearlescent layer is formed.
- SUBSTITUTE SHEET (RULE 26) The shell is placed in the habitat for the period of formation of nacre on the surface of the core of the required thickness.
- the core can be made of various materials: epoxy resin, acrylic, etc.
- the core can have any configuration.
- the pearl layer formed on the core represents a large pearl from 20 mm in size, which repeats the shape of the core.
- the pearl thickness is not less than 0.4 mm.
- One of the advantages of the proposed technology is that the entire core can be held inside the sink. Using the proposed method significantly reduces mortality • - molluscs, it is possible to cultivate a large pearl.
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- Farming Of Fish And Shellfish (AREA)
Abstract
Description
Способ культивирования жемчуга заданной формы и размера The method of cultivating pearls of a given shape and size
Изобретение относится к производству культивированного жемчуга и может быть использовано для получения крупного (от 20мм) жемчуга любой формы на пресноводных и морских жемчужницах, а также на моллюсках, образующих перламутровый слой.The invention relates to the production of cultured pearls and can be used to obtain large (from 20 mm) pearls of any shape on freshwater and sea pearls, as well as on mollusks that form the pearlescent layer.
Современное культивирование жемчуга базируется главным образом на трех основных методиках. Во-первых, сферические жемчужины всех размеров получаются по хорошо известной технологии вживление имплантанта ткани с использованием ядра на различных видах, как морских жемчужниц, так и пресноводных мидиях. Во-вторых, полусферические жемчужины размером 10-22 мм получаются с использованием так называемой технологии «блиcтepa», опять же на жемчужницах (устрицах) и пресноводных мидиях. В-третьих, жемчужины неправильной формы, или барокко, получаются на пресноводных моллюсках с использованием процесса многоразовых имплантаций без использования ядра.Modern pearl cultivation is mainly based on three main techniques. Firstly, spherical pearls of all sizes are obtained using the well-known technology of implanting a tissue implant using a core on various types of both sea pearls and freshwater mussels. Secondly, hemispherical pearls with a size of 10-22 mm are obtained using the so-called “blister” technology, again on pearls (oysters) and freshwater mussels. Thirdly, irregularly shaped pearls, or baroque, are obtained on freshwater mollusks using the process of reusable implantations without using a core.
В настоящее время для производства культивированного жемчуга используются в качестве материнского моллюска жемчужные устрицы.Currently, for the production of cultured pearls, pearl oysters are used as a mollusk.
Однако мировые водные пространства, где могут развиваться жемчужницы, крайне ограничены и соответственно производство и улов жемчужниц тоже ограничен.However, the world water spaces where pearl mussels can develop are extremely limited and, accordingly, the production and catch of pearl mussels is also limited.
Известны различные способы получения жемчуга, предусматривающие введение в мантийную полость моллюска ядра-затравки, представляющего собой обломки створок моллюска (Б.В.Зикеев. Переработка водного нерыбного сырья. M. 1950, стр. 212-21), кусочки мантии моллюска (заявка Японии 59-32088, 1984г), ассиметричные кристаллы карбонатов, полученные путем кристаллизации питательной среды, представляющей собой экстрапаллиальную жидкость из мантийного слоя моллюска (пат. РФ N°2183401).Various methods for producing pearls are known, involving introducing a seed core into the mantle cavity of the mollusk, which is fragments of mollusk flaps (B.V. Zikeev. Processing of non-fish raw water. M. 1950, pp. 212-21), slices of the mollusk mantle (Japanese application 59-32088, 1984), asymmetric crystals of carbonates obtained by crystallization of a nutrient medium, which is extrapallic fluid from the mantle layer of the mollusk (US Pat. RF N ° 2183401).
Известные способы дают возможность получить культивированный жемчуг, лишь по внешнему виду напоминающий естественный.Known methods make it possible to obtain cultured pearls, only in appearance resembling natural.
ЗАМЕНЯЮЩИЙ ЛИСТ (ПРАВИЛО 26) Известен способ введения ядра-затравки для выращивания жемчуга в мантийную полость моллюсков, предусматривающий раскрытие створок, прокалывание краевой ткани мантии моллюска и введение ядра-затравки (авт.свид. СССР N° 1701214). При этом с целью повышения надежности фиксации ядра-затравки и ускорения процесса регенерации ткани, прокалывание осуществляют Г-образными губками, перед прокалыванием ядро размещают между губками, пинцет вводят при прокалывании под углом к поперечной оси створки, а затем разжимают губки и раздвигают ткани моллюска с образованием Г-образного несквозного тоннеля для размещения ядра. Ткань под действием сил упругости мышц моллюска плотно фиксирует ядро, препятствуя его выпадению. После окончания операции при трансплантации ядра-затравки отверстие в ткани заклеивают клеем и моллюск возвращают в среду обитания. Недостатком такого введения ядра является травмирование моллюска и последующая его гибель.SUBSTITUTE SHEET (RULE 26) A known method of introducing a seed core for growing pearls in the mantle of the mollusks, comprising opening the cusps, piercing the edge tissue of the mantle of the mollusk and introducing the seed core (ed. Certificate. USSR N ° 1701214). Moreover, in order to increase the reliability of fixing the seed core and accelerate the process of tissue regeneration, the piercing is carried out with L-shaped sponges, before piercing, the core is placed between the lips, tweezers are inserted when piercing at an angle to the transverse axis of the leaflet, and then the lips are opened and the mollusk tissues are pushed apart with the formation of a L-shaped through hole tunnel to accommodate the core. The tissue under the action of the elastic forces of the muscles of the mollusk tightly fixes the core, preventing its loss. After the operation, during transplantation of the seed core, the hole in the tissue is sealed with glue and the mollusk is returned to the habitat. The disadvantage of this introduction of the kernel is the injury to the mollusk and its subsequent death.
Известно культивирование жемчуга на морском ушке семейства Галиотис (патент США 3871333). На репродуктивный орган морского ушка через перфорированное отверстие в раковине в репродуктивном органе осторожно укладывается округленный предмет, например, бусина, который образует ядро. Прежде чем перейти к этому этапу морское ушко выдерживается в морских условиях, по крайней мере, в течение двух недель. Желательно, чтобы закладка ядра производилась в период с ноября по февраль. Сразу же после закладки ядра отверстие в раковине полностью закрывается с помощью водоизолирующего связующего реактива или цемента и таким образом обработанное морское ушко выращивается в соответствующем аквариуме, после чего морское ушко вырабатывает перламутровое вещество вокруг ядра. В течение приблизительно 10- 12 месяцев нарастание перламутрового слоя становиться довольно толстым. Затем грубый жемчужный продукт извлекается из раковины и шлифуется до получения окончательного жемчужного продукта. Для реализации способа используют ядро в виде бусины из пластичной смолы, которая крепится к тонкой игле и уложена на пару дисков ( внутренний с меньшим диаметром и внешний с большим диаметром) с помощью которых закрывается отверстие в раковине. За счет иглы ядро фиксируется на раковине. После завершения культивирования в течениеCultivation of pearls on the abalone of the Galiotis family is known (US Pat. No. 3,871,333). A rounded object, for example, a bead that forms the core, is carefully placed on the reproductive organ of the abalone through a perforated hole in the shell in the reproductive organ. Before proceeding to this stage, the abalone is aged in marine conditions for at least two weeks. It is advisable that the kernel was laid in the period from November to February. Immediately after laying the core, the hole in the shell is completely closed with a waterproofing binder or cement, and the treated abalone is grown in a suitable aquarium, after which the abalone produces pearlescent material around the core. Within about 10-12 months, the growth of the pearlescent layer becomes quite thick. The coarse pearl product is then removed from the sink and polished until the final pearl product is obtained. To implement the method, a core is used in the form of a bead made of plastic resin, which is attached to a thin needle and laid on a pair of disks (internal with a smaller diameter and external with a large diameter) with which the hole in the sink is closed. Due to the needle, the core is fixed on the sink. After completion of cultivation for
ЗАМЕНЯЮЩИЙ ЛИСТ (ПРАВИЛО 26) предписанного периода морское ушко вынимается из воды и сферический протуберанец (ядро, покрытое слоем перламутра) отделяется от раковиныSUBSTITUTE SHEET (RULE 26) prescribed period, the abalone is removed from the water and the spherical prominence (the core covered with a layer of nacre) is separated from the shell
Недостатком метода является то, что полученный жемчуг не был крупным и круглым, а лишь полусферическим и мелким, при этом наблюдался большой процент гибели материнских моллюсков. Морское ушко является одностворчатым и требует большой осторожности и внимания, чем жемчужные устрицы, являющиеся двустворчатыми.The disadvantage of this method is that the resulting pearls were not large and round, but only hemispherical and small, while a large percentage of death of maternal mollusks was observed. The abalone is single-leaf and requires great care and attention than pearl oysters, which are bivalves.
Наиболее близким техническим решением к заявляемому способу является способ культивирования жемчуга заданной формы и размера, включающий образование отверстия в раковине моллюска, введение ядра внутрь моллюска через полученное отверстие, герметизацию раковины и формирование жемчужного слоя на ядре (пат США 5347951). Ядро имеет заданную конфигурацию и состоит из первой части, вокруг которой образуется перламутр при введении ядра внутрь моллюска через отверстие. Другая часть ядра представляет собой пробку с диском, которая прочно соединена с первой частью, и снабжена удерживающим средством на внешней стороне раковины для физического блокирования отверстия с достаточной степенью усилия. Желательно, чтобы внешняя поверхность удерживающего средства имела нарезки или наклонную или гиперболическую поверхность. Недостаток - невозможность культивирования крупного жемчуга, большая смертность моллюсков за счет отторжения инородного тела, много травмирующих операций недостаточная герметизация раковины после введения ядра-затравки. Уровень инфекционного заражения также сравнительно высок, главным образом из-за травматизма тканей.The closest technical solution to the claimed method is a method of culturing pearls of a given shape and size, including forming a hole in the mollusk shell, introducing the core into the mollusk through the hole obtained, sealing the shell and forming a pearl layer on the core (US Pat. No. 5,347,951). The core has a predetermined configuration and consists of the first part, around which mother-of-pearl is formed when the core is inserted into the mollusk through the hole. The other part of the core is a cork with a disk, which is firmly connected to the first part, and is equipped with retaining means on the outside of the sink to physically block the hole with a sufficient degree of force. Preferably, the outer surface of the holding means has cuts or an inclined or hyperbolic surface. The disadvantage is the impossibility of cultivating large pearls, the large mortality of mollusks due to rejection of a foreign body, many traumatic operations, insufficient sealing of the shell after the introduction of the seed core. The level of infection is also relatively high, mainly due to tissue injuries.
Цель предлагаемого технического решения - культивирования крупного жемчуга, снижение .смертности моллюсков за счет отсутствия отторжения инородного тела, надежность и эффективность способа культивирования жемчуга.The purpose of the proposed technical solution is the cultivation of large pearls, reducing the mortality of mollusks due to the absence of rejection of a foreign body, the reliability and effectiveness of the method of cultivating pearls.
Поставленная цель достигается за счет использования предлагаемого способа культивирования жемчуга заданной формы и размера, включающего образование отверстия в раковине моллюска, введение ядра внутрь моллюска через полученное отверстие, герметизацию отверстия раковины и последующее формирование жемчужного слоя на ядре, при этом при введении ядра внутрьThis goal is achieved through the use of the proposed method of culturing pearls of a given shape and size, including the formation of a hole in the mollusk shell, introducing the core into the mollusk through the hole obtained, sealing the shell hole and the subsequent formation of the pearl layer on the core, while introducing the core inside
ЗАМЕНЯЮЩИЙ ЛИСТ (ПРАВИЛО 26) моллюска на мантию осуществляют направленную компрессию с помощью ядра с постепенным и одновременным погружением ядра и мантии внутрь моллюска со скоростью 0,01 - 2 мм в сутки до формирования жемчугобразующего мешка из мантии, после чего направленную компрессию прекращают.SUBSTITUTE SHEET (RULE 26) mollusk on the mantle carry out directional compression using the core with the gradual and simultaneous immersion of the core and mantle inside the mollusk at a rate of 0.01 - 2 mm per day until a pearl-forming bag is formed from the mantle, after which the directed compression is stopped.
Скорость погружения ядра и мантии внутрь моллюска, определенная экспериментальным путем, обеспечивала жизнеспособность моллюска и целостность мантии.The rate of immersion of the core and mantle inside the mollusk, determined experimentally, ensured the viability of the mollusk and the integrity of the mantle.
Способ осуществляется следующим образом.The method is as follows.
Моллюсков сортируют по массе, очищают поверхность раковины от загрязнений. Затем в раковине моллюска вырезают обычно алмазным буром отверстие диаметром больше чем ядро. Закрепляют на раковине водозащитным клеем пластину с отверстием, на которой установлено ядро с механизмом, позволяющим осуществлять направленную компрессию. Отверстие и пластину герметизируют подводной эпоксидной замазкой или несколькими каплями суперклея . Начинают осуществлять погружение ядра и мантии внутрь моллюска за счет направленной компрессии. Постепенное и одновременное погружение ядра и мантии внутрь моллюска со скоростью 0,01 - 2 мм в сутки способствует формированию жемчугобразующего мешка из мантии. После образования мешка направленную компрессию прекращают и начинают формирование жемчужного слоя.Shellfish are sorted by weight, clean the surface of the sink from contamination. Then, in a mollusk shell, a hole with a diameter larger than the core is usually cut with a diamond drill. A plate with a hole is fixed on the sink with waterproof glue, on which the core is installed with a mechanism that allows for directed compression. The hole and plate are sealed with an underwater epoxy putty or a few drops of superglue. They begin to immerse the core and mantle inside the mollusk due to directional compression. The gradual and simultaneous immersion of the core and mantle inside the mollusk at a rate of 0.01 - 2 mm per day promotes the formation of a pearl-forming bag from the mantle. After the formation of the bag, directional compression is stopped and the formation of a pearl layer begins.
Жизнеспособность моллюска на первом этапе вживления ядра оценивается по его активности визуально, например, по активности мантии при ее раздражении, целостность мантии определяется также визуально, например, эндоскопическими методами.The viability of a mollusk at the first stage of implantation of a nucleus is assessed visually by its activity, for example, by the activity of the mantle upon irritation, the integrity of the mantle is also determined visually, for example, by endoscopic methods.
За счет использования заявляемого способа мантийная ткань постепенно перераспределяется и привыкает к инородному телу, адаптируется к нему. Процесс образования жемчугобразующего мешка из мантии длится в среднем в течении двух недель, при этом моллюск находится в аквариуме с морской водой. После того, как операция по внедрению ядра в моллюск закончена, моллюск вновь помещается в обычный подводный садок или ферму. Вторая стадия - образование перламутрового слоя - длиться в течении 3 - 6 месяцев и зависит от размера моллюска и размера ядра на котором формируется перламутровый слой.Through the use of the proposed method, mantle tissue is gradually redistributed and gets used to a foreign body, adapts to it. The process of forming a pearl-forming bag from the mantle lasts an average of two weeks, while the mollusk is in an aquarium with sea water. After the operation of introducing the core into the mollusk is completed, the mollusk is again placed in a conventional underwater cage or farm. The second stage - the formation of the pearlescent layer - lasts for 3-6 months and depends on the size of the mollusk and the size of the core on which the pearlescent layer is formed.
ЗАМЕНЯЮЩИЙ ЛИСТ (ПРАВИЛО 26) Раковину помещают в среду обитания на срок образования перламутра на поверхности ядра требуемой толщины. Ядро может изготавливаться из различных материалов: эпоксидная смола, акрил и др. Ядро может иметь любую конфигурацию.SUBSTITUTE SHEET (RULE 26) The shell is placed in the habitat for the period of formation of nacre on the surface of the core of the required thickness. The core can be made of various materials: epoxy resin, acrylic, etc. The core can have any configuration.
После формирования жемчужного слоя раковина извлекается из садка и ядро удаляется. Слой перламутра, образовавшийся на ядре, представляет крупную от 20 мм жемчужину, которая повторяет форму ядра. Толщина перламутра не менее 0,4 мм.After the formation of the pearl layer, the shell is removed from the cage and the core is removed. The pearl layer formed on the core represents a large pearl from 20 mm in size, which repeats the shape of the core. The pearl thickness is not less than 0.4 mm.
Одним из преимуществ, предлагаемой технологии является то, что все ядро может удерживаться внутри раковины. Используя заявляемый способ, значительно сокращается смертность •- моллюсков, появляется возможность культивировать крупные жемчужины.One of the advantages of the proposed technology is that the entire core can be held inside the sink. Using the proposed method significantly reduces mortality • - molluscs, it is possible to cultivate a large pearl.
ЗАМЕНЯЮЩИЙ ЛИСТ (ПРАВИЛО 26) SUBSTITUTE SHEET (RULE 26)
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| RU2008115630 | 2008-04-23 | ||
| RU2008115630/12A RU2374838C1 (en) | 2008-04-23 | 2008-04-23 | Method for cultivation of pearl with specified shape and size |
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| PCT/RU2009/000162 Ceased WO2009131491A1 (en) | 2008-04-23 | 2009-04-07 | Method for cultivating pearls of specified shape and size |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314905A (en) * | 2013-06-20 | 2013-09-25 | 北海市源龙珍珠有限公司 | Purplish red color pearl cultivating method |
| CN103329826A (en) * | 2013-06-20 | 2013-10-02 | 北海市源龙珍珠有限公司 | Sapphire blue colorful pearl cultivation method |
| CN104885985A (en) * | 2015-06-26 | 2015-09-09 | 海南大学 | Method for cultivating round pearl of penguin pearl shell |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1701214A1 (en) * | 1990-01-02 | 1991-12-30 | Ю.Я.Гороховский | Method for introduction of seed for growing pearl in shell mantle cavity |
| US5347951A (en) * | 1990-11-15 | 1994-09-20 | Pacific Pearl Culture Ltd. | Process for producing pearls in abalone and other shell-bearing molluska and nucleus used therewith |
| KR20010057027A (en) * | 1999-12-17 | 2001-07-04 | 대 일 이 | the cultivating method of sea-ear pearl |
| RU2183401C1 (en) * | 2001-01-09 | 2002-06-20 | Кулешин Виктор Александрович | Method to grow pearl |
-
2008
- 2008-04-23 RU RU2008115630/12A patent/RU2374838C1/en not_active IP Right Cessation
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2009
- 2009-04-07 WO PCT/RU2009/000162 patent/WO2009131491A1/en not_active Ceased
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1701214A1 (en) * | 1990-01-02 | 1991-12-30 | Ю.Я.Гороховский | Method for introduction of seed for growing pearl in shell mantle cavity |
| US5347951A (en) * | 1990-11-15 | 1994-09-20 | Pacific Pearl Culture Ltd. | Process for producing pearls in abalone and other shell-bearing molluska and nucleus used therewith |
| KR20010057027A (en) * | 1999-12-17 | 2001-07-04 | 대 일 이 | the cultivating method of sea-ear pearl |
| RU2183401C1 (en) * | 2001-01-09 | 2002-06-20 | Кулешин Виктор Александрович | Method to grow pearl |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314905A (en) * | 2013-06-20 | 2013-09-25 | 北海市源龙珍珠有限公司 | Purplish red color pearl cultivating method |
| CN103329826A (en) * | 2013-06-20 | 2013-10-02 | 北海市源龙珍珠有限公司 | Sapphire blue colorful pearl cultivation method |
| CN104885985A (en) * | 2015-06-26 | 2015-09-09 | 海南大学 | Method for cultivating round pearl of penguin pearl shell |
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| RU2374838C1 (en) | 2009-12-10 |
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