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WO2009125343A3 - Method for producing selenoproteins - Google Patents

Method for producing selenoproteins Download PDF

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Publication number
WO2009125343A3
WO2009125343A3 PCT/IB2009/051459 IB2009051459W WO2009125343A3 WO 2009125343 A3 WO2009125343 A3 WO 2009125343A3 IB 2009051459 W IB2009051459 W IB 2009051459W WO 2009125343 A3 WO2009125343 A3 WO 2009125343A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
intein
secis
polypeptide
selenocysteine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2009/051459
Other languages
French (fr)
Other versions
WO2009125343A2 (en
Inventor
Elias ARNÉR
Qing Cheng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2009125343A2 publication Critical patent/WO2009125343A2/en
Publication of WO2009125343A3 publication Critical patent/WO2009125343A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention describes a method for the production of recombinant selenoproteins containing an internal selenocysteine residue without the presence of other amino acids derived from the SECIS element. A first nucleic acid sequence encoding a first part of a protein to be formed is positioned upstream of an intein, with a UGA codon (which will be translated into a selenocysteine reside) located at or near the 3'-end of the first nucleic acid sequence. A SECIS sequence is then inserted into the 5'-end of the intein. A precursor protein is formed containing the first part of the protein, the intein (with the SECIS) and a second polypeptide. The intein (with SECIS) is excised from the precursor and the first and second polypeptides are joined by a peptide bond to form a protein with an internal selenocysteine residue, or alternatively, after excision of the intein, the first polypeptide is ligated to a third polypeptide (i.e. a polypeptide which was not part of the precursor protein) to form a protein with an internal selenocysteine residue. The protein does not include any amino acids derived from the SECIS apart from the selenocysteine residue.
PCT/IB2009/051459 2008-04-07 2009-04-07 Method for producing selenoproteins Ceased WO2009125343A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4297108P 2008-04-07 2008-04-07
US61/042,971 2008-04-07

Publications (2)

Publication Number Publication Date
WO2009125343A2 WO2009125343A2 (en) 2009-10-15
WO2009125343A3 true WO2009125343A3 (en) 2009-12-03

Family

ID=41057534

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2009/051459 Ceased WO2009125343A2 (en) 2008-04-07 2009-04-07 Method for producing selenoproteins

Country Status (1)

Country Link
WO (1) WO2009125343A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102286518B (en) * 2011-05-18 2013-07-17 南京大学 Method for constructing stringent T7 expression system host bacterium
CN114027430A (en) * 2021-11-25 2022-02-11 金腰燕（广州）食品有限公司 A health solid beverage containing nidus Collocaliae

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856131A (en) * 1997-02-24 1999-01-05 Incyte Pharmaceuticals, Inc. Human selenoprotein
WO2001012657A2 (en) * 1999-08-16 2001-02-22 Karolinska Innovations Ab Methods and means for selenoprotein expression
US20030100055A1 (en) * 2000-12-18 2003-05-29 Sheppard Paul O. Seleno-cysteine containing protein zsnk13

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856131A (en) * 1997-02-24 1999-01-05 Incyte Pharmaceuticals, Inc. Human selenoprotein
WO2001012657A2 (en) * 1999-08-16 2001-02-22 Karolinska Innovations Ab Methods and means for selenoprotein expression
US20030100055A1 (en) * 2000-12-18 2003-05-29 Sheppard Paul O. Seleno-cysteine containing protein zsnk13

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHENG QING ET AL: "Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C.", NATURE PROTOCOLS 2006, vol. 1, no. 2, 2006, pages 604 - 613, XP001539555, ISSN: 1750-2799 *
ECKENROTH B ET AL: "Semisynthesis and characterization of mammalian thioredoxin reductase", BIOCHEMISTRY 20060425 AMERICAN CHEMICAL SOCIETY US, vol. 45, no. 16, 25 April 2006 (2006-04-25), pages 5158 - 5170, XP002546995 *
HONDAL ROBERT J: "Incorporation of selenocysteine into proteins using peptide ligation", PROTEIN AND PEPTIDE LETTERS, BENTHAM SCIENCE PUBLISHERS, SCHIPHOL, NL, vol. 12, no. 8, 1 November 2005 (2005-11-01), pages 757 - 764, XP009122816, ISSN: 0929-8665 *
IRONS ROBERT ET AL: "Both selenoproteins and low molecular weight selenocompounds reduce colon cancer risk in mice with genetically impaired selenoprotein expression", JOURNAL OF NUTRITION, vol. 136, no. 5, May 2006 (2006-05-01), pages 1311 - 1317, XP002547141, ISSN: 0022-3166 *
JOHANSSON LINDA ET AL: "Exploiting the 21st amino acid - purifying and labeling proteins by selenolate targeting", NATURE METHODS, vol. 1, no. 1, October 2004 (2004-10-01), pages 61 - 66, XP009122810, ISSN: 1548-7091 *
MATHYS S ET AL: "Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation", GENE, ELSEVIER, AMSTERDAM, NL, vol. 231, no. 1-2, 29 April 1999 (1999-04-29), pages 1 - 13, XP004166772, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
WO2009125343A2 (en) 2009-10-15

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