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WO2009123173A1 - Récipient de culture, procédé de culture et appareil de culture - Google Patents

Récipient de culture, procédé de culture et appareil de culture Download PDF

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Publication number
WO2009123173A1
WO2009123173A1 PCT/JP2009/056612 JP2009056612W WO2009123173A1 WO 2009123173 A1 WO2009123173 A1 WO 2009123173A1 JP 2009056612 W JP2009056612 W JP 2009056612W WO 2009123173 A1 WO2009123173 A1 WO 2009123173A1
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WO
WIPO (PCT)
Prior art keywords
culture
medium
cells
partition member
flow path
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2009/056612
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English (en)
Japanese (ja)
Inventor
郷史 田中
亮 末永
庸一 石崎
偉子 時田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyo Seikan Group Holdings Ltd
Original Assignee
Toyo Seikan Kaisha Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2008095557A external-priority patent/JP5309658B2/ja
Priority claimed from JP2008095556A external-priority patent/JP5309657B2/ja
Application filed by Toyo Seikan Kaisha Ltd filed Critical Toyo Seikan Kaisha Ltd
Publication of WO2009123173A1 publication Critical patent/WO2009123173A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02—Percolation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate

Definitions

  • the present invention relates to a culture vessel, a culture method, and a culture apparatus for culturing cells, tissues, microorganisms, and the like.
  • the medium and cells are first placed in the flask and cultured, and the medium is added and cultured in conjunction with cell growth.
  • the cells were transferred to a large bag, etc., and culture, medium supplementation, and planting were repeated to mass-culture cells.
  • the reason why the cells are transplanted when mass-culturing the cells is that the cell growth is suppressed when the cell density at the start of the culture is low.
  • the cell culture instrument described in Patent Document 1 is capable of expanding the culture region stepwise by using a member for preventing the flow of the culture solution in the bag. For this reason, it is not necessary to transplant cultured cells, and the risk of contamination can be reduced.
  • the culture container described in Patent Document 2 includes external sorting means for dividing a single bag-like sealed chamber into sub-compartments.
  • external partitioning means for dividing a single bag-like sealed chamber into sub-compartments.
  • a plurality of subcompartments can be created by tightening a part of the bag-like sealed chamber, and a sufficiently small starting environment can be provided to ensure a suitable cell density at the start of culture.
  • the culture region can be expanded stepwise in an environment where such contamination does not occur.
  • JP 2000-125848 A Japanese Patent No. 2981684
  • the present invention has been considered in view of the above circumstances, and in addition of a medium accompanying cell growth and expansion of a culture region, while maintaining an optimum culture density and realizing efficient culture, improvement in workability
  • An object of the present invention is to provide a culture vessel, a culture method, and a culture apparatus that can reduce contamination risk and achieve space saving.
  • the culture container of the present invention is a culture container for culturing cells by enclosing cells and a medium, and connects at least the front part and the rear part of the accommodating part of the culture container,
  • the configuration includes one flow path.
  • This storage part is separated into a front part and a rear part by a partition member, and one of the front part and the rear part is used as a culture part for culturing, and the other part is used as a medium supply part for storing a supplementary medium for the culture part.
  • the culture container is filled with a medium and further filled with cells, microorganisms, multicellular organisms, tissues, organs, and the like. Further, by providing a check valve in the flow path, it is possible to prevent the culture medium from flowing back from the culture section to the culture medium supply section. It is also preferable to use a pump for supplying a medium from the medium supply unit to the culture unit. Moreover, it is preferable to form a flow path in at least a part of the accommodating portion of the culture container, and it is also preferable to form the flow path with one or more tubes.
  • the culture container of this invention moves the partition member to the culture-medium supply part side, and the culture medium in a culture-medium supply part is supplied to a culture part via a flow path.
  • the culture container of the present invention is provided with one or two or more culture units for culturing, and one or two or more culture medium supply media for supplementing the culture unit, with the accommodating part of the culture container being a partition member. It is also preferable to include at least one flow path that is separated into a part and connects the culture part and the medium supply part.
  • the culture method of the present invention is a culture method for culturing cells by enclosing cells and a medium in a culture vessel, wherein one or two or more for culturing the storage portion of the culture vessel with a partition member Separating into a culture part and one or two or more medium supply parts containing a supplementary medium for the culture part, and moving the partition member to the medium supply part side to supply the culture medium in the medium supply part to the culture part As a way to do.
  • a roller can be used as the partition member, and supply of the culture medium to the culture unit in the culture medium supply unit can be performed via one or more flow paths connecting the culture medium supply unit and the culture unit.
  • the flow path may be formed by a slit provided in a roller as a partition member, or may be formed by one or two or more bypass tubes that connect the medium supply unit and the culture unit.
  • the channel can also be formed in the culture container by adhering a part of the upper and lower surfaces of the culture container.
  • the culture apparatus of the present invention is a culture apparatus for culturing cells by enclosing the cells and culture medium in a culture container, and the culture container and one or two or more for culturing the housing part of the culture container And a partition member that separates the culture part into one or more medium supply parts that contain a supplementary medium for the culture part, and the medium in the medium supply part when the partition member is moved to the medium supply part side. Is provided with a flow path for supplying to the culture unit.
  • a roller can be used as the partition member, and the flow path can be formed by a part of the roller in the circumferential direction or a predetermined recess provided in the axial direction of the roller.
  • the concave portion includes a gap where the medium provided on the roller can move, and the shape thereof is not particularly limited. Furthermore, when the flow path is formed by adhering part of the upper and lower surfaces of the bypass tube or the culture vessel, it is preferable to provide a pump or a valve on the flow path.
  • a culture region can be continuously expanded and a medium can be added using a single culture vessel. Therefore, efficient culture can be performed while maintaining an optimal culture density, and it is possible to reduce damage to cells, increase work efficiency, and reduce contamination risk.
  • FIG. 1 is a diagram showing a culture vessel used in the present embodiment.
  • the culture container 1 shown in FIG. 1A includes a storage unit for filling the culture medium 10 and has a flow path that connects the front and back of the storage unit.
  • an adhesive portion 2 is formed in the vicinity of one side portion of the housing portion to bond the upper surface and the lower surface of the bag constituting the container in the front-rear direction (vertical direction in FIG. 1A). It is.
  • the bonding portion 2 does not reach the front end and the rear end of the bag. Accordingly, the flow path 3 is formed by the adhesive portion 2 along one side end of the bag.
  • the bonding portion 2 is obtained by melting the upper surface and lower surface of the bag with heat and press-bonding them, and bonding them together by heat sealing.
  • the culture container 1 when the culture container 1 is configured as described above, when cells grow in the front part of the storage part and a culture medium is added, the culture medium in the rear part of the storage part is moved to the flow path 3 by moving a partition member to be described later. Can be supplied to the front part of the accommodating part.
  • the culture vessel 1 can have a configuration in which two or more tubes 4 connected to the outside of the vessel are connected. Of these tubes 4, one is for injection for injecting cells and medium to be cultured into the culture container 1 from the outside, and the other is for recovering cultured cells and medium from the culture container 1. Can be used. Further, as shown in FIG. 1, when three tubes 4 are attached, the third tube can be used for sampling for taking out cultured cells and culture media from the culture vessel 1 as samples.
  • the culture vessel 1 is preferably formed into a bag shape (bag shape) using a soft wrapping material.
  • a soft packaging material By using a soft packaging material, flexibility and softness can be imparted to the culture vessel.
  • the soft packaging material include, for example, Japanese Patent Application Laid-Open No. 2002-255277 (a food packaging body using a soft packaging material film sheet and a method for taking out food), and Japanese Patent Application Laid-Open No. 2004-323077 (a pressure extraction type bag shape). Those described in (Containers) can be used.
  • the culture vessel 1 has gas permeability necessary for cell culture, and a part or all of the culture vessel 1 is transparent so that the contents can be confirmed.
  • the culture vessel material satisfying such conditions include polyolefin, ethylene-vinyl acetate copolymer, styrene elastomer, olefin thermoplastic elastomer, polyester thermoplastic elastomer, silicone thermoplastic elastomer, silicone rubber and the like. Can be mentioned.
  • the culture container 1 of the present embodiment only needs to have at least one flow path that connects the front and rear of the accommodating portion.
  • FIG. 1 (a) bypasses such as FIGS. 1 (b) to 1 (f)
  • FIGS. 1 (b) to 1 (f) A configuration using the tube 5 is also possible. That is, the culture container 1 shown in FIG. 1B has the front side edge (the tube side for collecting the culture medium) and the rear side edge (the opposite side of the tube for collecting the culture medium) of the housing portion by the bypass tube 5. It is connected.
  • Examples of the material of the tube 4 and the bypass tube 5 include silicone rubber, soft vinyl chloride resin, polybutadiene resin, ethylene-vinyl acetate copolymer, chlorinated polyethylene resin, olefin-based thermoplastic elastomer, polyurethane-based thermoplastic elastomer.
  • SBS styrene / butadiene / styrene
  • SIS styrene / isoprene / styrene
  • SEBS styrene / ethylene / butylene / styrene
  • SEPS Styrene, ethylene, propylene, styrene
  • the culture container 1 can also be set as the structure which connected the bypass tube 5 to the front part side edge of the accommodating part from the rear-end part of an accommodating part.
  • the culture medium can be more efficiently moved from the rear portion of the storage portion to the bypass tube 5 by moving the partition member.
  • the culture vessel 1 is connected to the bypass tube 5 from the rear end of the storage portion to the front side edge of the storage portion, and the storage to which the bypass tube 5 is connected. It is also preferable to have a configuration in which both corners of the rear part of the part are cut to seal the housing part. If it does in this way, it will become possible to move a culture medium to the bypass tube 5 more efficiently from the rear part of a storage part by moving a partition member. Moreover, the control of the expansion of the culture area of the front part of the accommodating part is more accurate by cutting the corner on the opposite side of the part where the bypass tube 5 is connected at the front side edge of the accommodating part and sealing the accommodating part. And the culture efficiency can be kept high.
  • the culture vessel 1 has a configuration in which the bypass tube 5 is connected symmetrically from the rear end portion of the housing portion to the front side edge of the housing portion. It is also preferable. In this way, when the culture medium is supplied to the front part of the storage unit by moving the partition member, the culture medium can be spread evenly on the left and right, so that the culture efficiency can be further improved. Become. Furthermore, the culture vessel 1 can be configured to have both the flow path 3 as shown in FIG. 1A and the bypass tube 5 as shown in FIGS. 1B to 1F.
  • valve 6 is provided in the bypass tube 5 of the culture vessel 1 shown in FIG. 1 (b)
  • pump 7 is provided in the bypass tube 5 of the culture vessel 1 shown in FIG. 1 (c).
  • FIG. 2 is an example of the culture method using the culture container shown in FIG. 1 (a).
  • the housing part of the culture container 1 is divided into a front part and a rear part using a partition member 8, and the front part is cultured.
  • a configuration is shown in which a culture unit 11 for performing the above and a medium supply unit 12 for containing a supplementary medium in the rear part are shown.
  • the partition member 8 is not particularly limited as long as it can separate the culture vessel 1 into the culture unit 11 side and the culture medium supply unit 12 side and block the movement of the culture medium between them. Etc. can be used.
  • the partition member 8 When cells grow in the culture unit 11 and a culture medium is added from the culture medium supply unit 12 to the culture unit 11, the partition member 8 is inserted into the rear end of the storage unit by removing the clip of the flow path 3 or opening the valve. Move towards.
  • a check valve or the like can be used. If it does in this way, the internal pressure of the culture-medium supply part 12 will become high, and a culture medium will be added to the culture part 11 via the flow path 3.
  • the culture container 1 of this embodiment it is possible to adjust continuously the quantity of the culture medium to move by using the partition member 8, a valve
  • FIG. 3 is an example of the culture method using the culture container shown in FIG. 1B, and the storage part of the culture container 1 is divided into a front part and a rear part using a partition member 8, and the front part is cultured.
  • a configuration is shown in which a culture unit 11 for performing the above and a medium supply unit 12 for containing a supplementary medium in the rear part are shown.
  • a valve 6 is attached to the central portion of the flow path formed by the bypass tube 4, and the culture unit 11 side and the culture medium supply unit 12 side are connected so as to be shut off and communicated.
  • a check valve or the like can be used.
  • the partition member 8 is not particularly limited as long as it can separate the culture vessel 1 into the culture unit 11 side and the culture medium supply unit 12 side and block the movement of the culture medium between them. Etc. can be used.
  • the valve 6 When cells grow in the culture unit 11 and the culture medium is added from the culture medium supply unit 12 to the culture unit 11, the valve 6 is opened and the partition member 8 is moved toward the rear end of the storage unit. If it does in this way, the internal pressure of the culture medium supply part 12 will become high, and a culture medium will be added to the culture part 11 via the bypass tube 4. FIG. After the addition, the valve is closed to shut off the culture unit 11 and the medium supply unit 12.
  • the amount of the moving medium can be continuously adjusted by using the partition member 8 and the valve 6 as described above. Further, the partition member 8 is moved toward the rear end of the accommodating portion in accordance with the cell growth rate, and a constant amount of medium is actively sent to the culturing portion 11 by using the pump 7, so that it is continuously continuous. It is also possible to supply a medium.
  • the amount of the moving medium can be continuously adjusted, and the density of the cells to be cultured can be maintained within the optimum range for the culture.
  • FIG. 4 is a diagram showing experimental conditions for confirming the optimum cell density range for culture.
  • FIG. 5 is a diagram showing the multiplication factor with respect to the culture time for each cell density.
  • FIG. 6 is an enlarged view of a part of the diagram showing the multiplication factor with respect to the culture time for each cell density.
  • FIG. 7 is a diagram showing the optimum cell density dependency in cell culture.
  • culture bags adjusted to cell densities of 4000, 10,000, 40,000, 120,000, and 200,000 (cells / ml) were prepared under the conditions shown in FIG.
  • an LLDPE bag thinness: 100 ⁇ m
  • a back size of 50 ⁇ 80 (mm) were used as the culture bag.
  • RPMI1640 (invitrogen) 50 ml was used as a medium
  • Jurkat E6.1 strain human leukemia T lymphoma
  • FIG. 6 is an enlarged view of the encircled portion in FIG. As shown in FIG. 6, after 24 hours from the start of the culture, the growth rate of the culture bag having a low cell density (4000, 10,000 cells / ml) is indicated by the culture bag having a high cell density (120,000, 200,000 cells). / Ml) of the growth rate is 1/2 or less.
  • FIG. 7 summarizes the optimum cell density dependence in such cell culture.
  • the culture method of the present embodiment it is possible to add a medium and continuously expand a culture region using a single culture container. Therefore, efficient culture can be performed while maintaining an optimal culture density, and it is possible to reduce damage to cells, increase work efficiency, and reduce contamination risk.
  • cell culture can be easily automated by controlling the movement of the partition member and the valves and pumps of the flow path.
  • FIG. 8 is a diagram showing a culture apparatus in the present embodiment.
  • the culture apparatus in the present embodiment includes a culture container 1 and a roller 8.
  • FIG. 8A shows a state in which the accommodating portion of the culture vessel 1 is partitioned into a culture portion 11 and a medium supply portion 12 by the roller 8.
  • the roller 8 is provided with a slit forming portion 8a over a predetermined angle in the circumferential direction (about 100 ° in the example of FIG. 8).
  • a plurality of slit forming portions 8a are provided in the axial direction of the roller.
  • the roller 8 partitions the accommodating portion by a portion having no slit and rotates to form the flow path 3 by the slit forming portion 8a when the slit forming portion 8a is positioned on the culture vessel 1. Thereby, the culture medium in the culture medium supply unit 12 can be supplied to the culture unit 11.
  • the amount of medium to be added from the medium supply unit 12 to the culture unit 11 is adjusted by the diameter of the roller, the rotation speed of the roller, the formation angle on the circumference of the slit, and the number of slit formation units 8a formed in the axial direction. can do.
  • the surface material forming the outer peripheral surface of the roller 8 for example, one or two or more materials of urethane rubber, silicone rubber, and thermoplastic resin can be used.
  • the hardness of the surface material can be at least A90 (JIS K 6253) or less.
  • the hardness A90 indicates a value A90 measured with a durometer type A based on JIS K 6253.
  • the structure of the roller 8 is not limited to that shown in FIG. 8, and can be changed within a range in which the same effect can be obtained.
  • the cross section of the slit forming portion 8a can be formed as shown in FIGS. 9 (a) to 9 (c).
  • the cross section in the radial direction of the roller 8 may be elliptical as shown in FIG. In this way, by making the major axis direction of the ellipse perpendicular to the culture vessel 1, the movement of the medium between the culture unit 11 and the culture medium supply unit 12 can be prevented, and the major axis direction of the ellipse is set to the culture vessel 1.
  • the culture medium can be supplied from the culture medium supply unit 12 to the culture unit 11 by being parallel to each other.
  • FIGS. 9B and 9C it is possible to obtain the same effect by making the cross section in the radial direction of the roller 8 into a star shape or a petal shape.
  • two rollers 8 may be provided in the culture apparatus and pressed from both sides of the front and back surfaces of the culture vessel 1. Further, various modifications such as providing a slit on only one of the rollers are possible.
  • the culture method of the present embodiment it is possible to add a medium and continuously expand a culture region using a single culture container. Therefore, efficient culture can be performed while maintaining an optimal culture density, and it is possible to reduce damage to cells, increase work efficiency, and reduce contamination risk.
  • efficient culture can be performed while maintaining an optimal culture density, and it is possible to reduce damage to cells, increase work efficiency, and reduce contamination risk.
  • by counting the cell density in the culture part and controlling the movement of the roller so that the cell density is in a certain range it is possible to easily automate the cell culture.
  • Example 1 Cell culture was performed using a culture vessel 1 provided with a bypass tube 5 that constitutes a flow path for replenishing the medium shown in FIG.
  • the culture vessel 1 was an LLDPE bag (thickness 100 ⁇ m, size 145 ⁇ 295 mm), and the bypass tube 5 was a silicon tube (TYGON 3355L: inner diameter 4 mm).
  • RPMI1640 invitrogen
  • Jurkat E6.1 strain human leukemia T lymphoma
  • the culture part area extends from 170cm 2 to 430 cm 2, an enlarged view of the media amounts to cell density of 1.0 ⁇ 10 5 ⁇ 8.6 ⁇ 10 5 cells / ml in 100 ⁇ 500 ml.
  • the above culture conditions are shown in FIG.
  • Example 1 the initial culture density is set to be relatively high by setting the initial culture area to 170 cm 2 and the medium amount to 100 ml. And as shown in FIG.12 and FIG.13, the culture
  • the culture was performed for 120 hours in this manner, and the cultured cells were collected. The number of cells collected was 4.3 ⁇ 10 8 cells. The result is shown in FIG.
  • Example 1 Cell culture was performed using a conventional culture vessel without a flow path as it was without using a partition member or the like. The same culture container, medium and seeded cells as those in Example 1 were used. Then, the same number of cells as in Example 1 was seeded, the culture area was fixed at 430 cm 2 , and the medium amount was fixed at 500 ml for 120 hours, and the cultured cells were collected. The number of cells collected was 3.3 ⁇ 10 8 cells.
  • FIG. 11 shows the culture conditions
  • FIG. 12 and FIG. 13 show changes in the area of the culture part
  • FIG. 14 shows the experimental results.
  • Example 1 As shown in FIG. 14, the number of cells seeded in Example 1 and Comparative Example 1 was the same at 1.0 ⁇ 10 7 cells, but the number of recovered cells was 4.3 ⁇ 10 8 cells in Example 1, Comparative Example 1 is 3.3 ⁇ 10 8 cells, and Example 1 is about 30% more than Comparative Example 1.
  • Example 1 while expanding the culture portion in small increments in accordance with the growth of the cells, repeating the addition of the medium, the culture is performed without greatly changing the cell density from the start of the culture to the end of the culture. As a result, it was confirmed that the culture environment can be kept uniform in the optimum state as much as possible, and the cell culture speed can be increased. Further, as in Comparative Example 1, when the culture density at the start is low, a certain amount of time is required until an environment suitable for cell growth is reached. Therefore, when the culture density at the start is higher It was confirmed that the culture rate of the cells was slow compared to.
  • the present invention is not limited to the above embodiment, and that various modifications can be made within the scope of the present invention.
  • the partition member 8 is automatically moved continuously from the start of culture to the end of the culture in small amounts, or two or more partition members
  • the culture vessel can be appropriately changed, for example, to have a configuration including two or more culture units or medium supply units.
  • the present invention can be suitably used in the fields of biopharmaceuticals, regenerative medicine, immunotherapy and the like that require culturing a large amount of cells.

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Abstract

Selon l'invention, dans la culture de cellules, de tissus, de microorganismes et autres, on maintient une densité de culture optimale pour permettre une culture efficace. En même temps, la détérioration des cellules est réduite, le rendement du processus est amélioré et le risque de contamination est réduit. L'invention concerne un récipient de culture de cellules (1) renfermant des cellules et un milieu. Le récipient de culture (1) comporte au moins un canal reliant la partie avant de l'élément de stockage à sa partie arrière. L'élément de stockage est subdivisé en une partie avant et une partie arrière à l'aide d'un élément de paroi de séparation (8). La partie avant ou la partie arrière est utilisée comme récipient de culture (11) pour réaliser la culture, tandis que l'autre partie est employée comme récipient de fourniture de milieu (12) et contient le milieu de remplacement à délivrer à la partie dédiée à la culture (11). Lorsque l'on décale la paroi de séparation (8) vers le côté correspondant à la partie dédiée à la fourniture de milieu (12), le milieu présent dans cette partie (12) est délivré à la partie (11) dédiée à la culture des cellules. Un rouleau peut être utilisé en tant qu'élément de paroi de séparation (8). Le milieu est délivré à la partie dédiée à la culture (11) via le canal reliant celle-ci à la partie dédiée à la fourniture de milieu (12).
PCT/JP2009/056612 2008-04-01 2009-03-31 Récipient de culture, procédé de culture et appareil de culture Ceased WO2009123173A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2008-095557 2008-04-01
JP2008095557A JP5309658B2 (ja) 2008-04-01 2008-04-01 培養方法、及び培養装置
JP2008095556A JP5309657B2 (ja) 2008-04-01 2008-04-01 培養容器
JP2008-095556 2008-04-01

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102286375A (zh) * 2010-06-18 2011-12-21 中国科学院大连化学物理研究所 一种产油微生物的培养方法
WO2013088537A1 (fr) * 2011-12-14 2013-06-20 株式会社日立製作所 Récipient de culture cellulaire, et dispositif de sous-culture cellulaire automatisé et procédé de sous-culture cellulaire l'utilisant
WO2016104452A1 (fr) * 2014-12-25 2016-06-30 オリンパス株式会社 Appareil de culture de cellules et sac de culture de cellules
WO2016121292A1 (fr) * 2015-01-30 2016-08-04 東洋製罐グループホールディングス株式会社 Dispositif de culture cellulaire et procédé de culture cellulaire
CN106170540A (zh) * 2014-05-09 2016-11-30 东洋制罐集团控股株式会社 多腔室培养容器以及细胞培养方法
JP2019522978A (ja) * 2016-07-05 2019-08-22 ゼネラル・エレクトリック・カンパニイ 調節容積式の細胞培養のための装置および方法

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JPH0613499U (ja) * 1992-07-24 1994-02-22 積水化学工業株式会社 培養容器
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CN107109338A (zh) * 2014-12-25 2017-08-29 奥林巴斯株式会社 细胞培养装置和细胞培养袋
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JP2019522978A (ja) * 2016-07-05 2019-08-22 ゼネラル・エレクトリック・カンパニイ 調節容積式の細胞培養のための装置および方法
JP7026990B2 (ja) 2016-07-05 2022-03-01 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 調節容積式の細胞培養のための装置および方法
US11566218B2 (en) 2016-07-05 2023-01-31 Global Life Sciences Solutions Usa Llc Apparatus and methods for adjustable volume cell culture

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