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WO2009117522A3 - Séquenceur d'adn à base de nanopores et de nanotubes de carbone et séquenceur de reconnaissance série - Google Patents

Séquenceur d'adn à base de nanopores et de nanotubes de carbone et séquenceur de reconnaissance série Download PDF

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Publication number
WO2009117522A3
WO2009117522A3 PCT/US2009/037570 US2009037570W WO2009117522A3 WO 2009117522 A3 WO2009117522 A3 WO 2009117522A3 US 2009037570 W US2009037570 W US 2009037570W WO 2009117522 A3 WO2009117522 A3 WO 2009117522A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequencer
nanopore
carbon nanotube
based dna
nanotube based
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/037570
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English (en)
Other versions
WO2009117522A2 (fr
Inventor
Stuart Lindsay
Jin He
Peiming Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Arizona
Arizona State University ASU
Original Assignee
University of Arizona
Arizona State University ASU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Arizona, Arizona State University ASU filed Critical University of Arizona
Priority to US12/920,809 priority Critical patent/US8628649B2/en
Publication of WO2009117522A2 publication Critical patent/WO2009117522A2/fr
Priority to US13/122,104 priority patent/US8968540B2/en
Priority to PCT/US2009/059693 priority patent/WO2010042514A1/fr
Publication of WO2009117522A3 publication Critical patent/WO2009117522A3/fr
Anticipated expiration legal-status Critical
Priority to US14/577,715 priority patent/US9593372B2/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Nanotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Cette invention concerne des systèmes, des dispositifs et des procédés pour identifier des biopolymères, tels que des brins d'ADN, au fur et à mesure qu'ils traversent un élément de constriction tel qu'un nanopore de nanotube de carbone. Plus particulièrement, l'invention concerne des systèmes, des dispositifs et des procédés dans lesquels un fragment qui vient d'être transloqué du biopolymère forme un circuit électrique temporaire entre le nanopore du nanotube de carbone et une seconde électrode, qui peut également être un nanotube. En outre, l'invention concerne des systèmes, des dispositifs et des procédés dans lesquels l'élément de constriction est pourvu d'une unité fonctionnalisée qui, conjointement avec un fragment qui vient d'être transloqué du biopolymère, forme un circuit électrique temporaire qui peut être utilisé pour caractériser ledit fragment du biopolymère.
PCT/US2009/037570 2008-03-18 2009-03-18 Séquenceur d'adn à base de nanopores et de nanotubes de carbone et séquenceur de reconnaissance série Ceased WO2009117522A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/920,809 US8628649B2 (en) 2008-03-18 2009-03-18 Nanopore and carbon nanotube based DNA sequencer and a serial recognition sequencer
US13/122,104 US8968540B2 (en) 2008-10-06 2009-10-06 Trans-base tunnel reader for sequencing
PCT/US2009/059693 WO2010042514A1 (fr) 2008-10-06 2009-10-06 Séquenceur d'adn basé sur des nanopores et des nanotubes de carbone et éléments de reconnaissance sériels
US14/577,715 US9593372B2 (en) 2008-10-06 2014-12-19 Nanopore based sequencer

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US3764708P 2008-03-18 2008-03-18
US61/037,647 2008-03-18
US8300108P 2008-07-23 2008-07-23
US61/083,001 2008-07-23
US10301908P 2008-10-06 2008-10-06
US61/103,019 2008-10-06

Publications (2)

Publication Number Publication Date
WO2009117522A2 WO2009117522A2 (fr) 2009-09-24
WO2009117522A3 true WO2009117522A3 (fr) 2010-04-22

Family

ID=41091526

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/037570 Ceased WO2009117522A2 (fr) 2008-03-18 2009-03-18 Séquenceur d'adn à base de nanopores et de nanotubes de carbone et séquenceur de reconnaissance série

Country Status (2)

Country Link
US (1) US8628649B2 (fr)
WO (1) WO2009117522A2 (fr)

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Also Published As

Publication number Publication date
US20110120868A1 (en) 2011-05-26
US8628649B2 (en) 2014-01-14
WO2009117522A2 (fr) 2009-09-24

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