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WO2009116840A3 - Chemical reagents regulating stem cell fate - Google Patents

Chemical reagents regulating stem cell fate Download PDF

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Publication number
WO2009116840A3
WO2009116840A3 PCT/KR2009/001443 KR2009001443W WO2009116840A3 WO 2009116840 A3 WO2009116840 A3 WO 2009116840A3 KR 2009001443 W KR2009001443 W KR 2009001443W WO 2009116840 A3 WO2009116840 A3 WO 2009116840A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cell
chemical reagents
cell fate
present
regulating stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2009/001443
Other languages
French (fr)
Other versions
WO2009116840A2 (en
Inventor
Dong Wook Kim
Ki-Chul Hwang
Sung Hou Kim
Ji Young Kim
Woo Chul Chang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industry Academic Cooperation Foundation of Yonsei University
Original Assignee
Industry Academic Cooperation Foundation of Yonsei University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industry Academic Cooperation Foundation of Yonsei University filed Critical Industry Academic Cooperation Foundation of Yonsei University
Publication of WO2009116840A2 publication Critical patent/WO2009116840A2/en
Publication of WO2009116840A3 publication Critical patent/WO2009116840A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurology (AREA)
  • Rheumatology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention relates to a method for inducing differentiation of a stem cell, which comprises culturing the stem cell in the presence of an inhibitor to protein kinase A or Rho kinase. The present invention provides novel small molecules having isoquinolinesulfonamide scaffold to effectively induce differentiation of stem cells. The present invention allows for directed differentiation of stem cells to a specific cell type.
PCT/KR2009/001443 2008-03-21 2009-03-20 Chemical reagents regulating stem cell fate Ceased WO2009116840A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3842208P 2008-03-21 2008-03-21
US61/038,422 2008-03-21

Publications (2)

Publication Number Publication Date
WO2009116840A2 WO2009116840A2 (en) 2009-09-24
WO2009116840A3 true WO2009116840A3 (en) 2009-12-30

Family

ID=41091423

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/001443 Ceased WO2009116840A2 (en) 2008-03-21 2009-03-20 Chemical reagents regulating stem cell fate

Country Status (1)

Country Link
WO (1) WO2009116840A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9068166B2 (en) 2010-09-16 2015-06-30 Industry-Academic Cooperation Foundation Yonsei University Use of compounds for inducing differentiation of mesenchymal stem cells to chondrocytes

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011062013A1 (en) * 2009-11-18 2011-05-26 国立大学法人徳島大学 Inducer of selective differentiation into neural stem cells and/or neural precursor cells
KR20120029225A (en) * 2010-09-16 2012-03-26 파미셀 주식회사 Use of ptk inhibitor for inducing differentiation of mesenchymal stem cells to cardiogenic cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008089351A1 (en) * 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008089351A1 (en) * 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEN, S. ET AL.: "Self renewal of embryonic stem cells by a small molecule.", PROC. NATL. ACAD. SCI. U. S. A., vol. 103, no. 46, 6 November 2006 (2006-11-06), pages 17266 - 17271 *
SAXE, J. P. ET AL.: "A phenotypic small-molecule screen identifies an orphan ligand-receptor pair that regulates neural stem cell differentiation.", CHEM. BIOL., vol. 14, no. 9, September 2007 (2007-09-01), pages 1019 - 1030 *
SCHUGAR, R. C. ET AL.: "Small molecules in stem cell self-renewal and differentiation.", GENE. THER., vol. 15, no. 2, 8 November 2007 (2007-11-08), pages 126 - 135 *
WATANABE, K. ET AL.: "A ROCK inhibitor permits survival of dissociated human embryonic stem cells.", NAT. BIOTECHNOL., vol. 25, no. 6, 27 May 2007 (2007-05-27), pages 681 - 686 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9068166B2 (en) 2010-09-16 2015-06-30 Industry-Academic Cooperation Foundation Yonsei University Use of compounds for inducing differentiation of mesenchymal stem cells to chondrocytes

Also Published As

Publication number Publication date
WO2009116840A2 (en) 2009-09-24

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