[go: up one dir, main page]

WO2009154804A3 - Sondes d'hybridation d'acides nucléiques peptidiques à fluorophores jumeaux - Google Patents

Sondes d'hybridation d'acides nucléiques peptidiques à fluorophores jumeaux Download PDF

Info

Publication number
WO2009154804A3
WO2009154804A3 PCT/US2009/003729 US2009003729W WO2009154804A3 WO 2009154804 A3 WO2009154804 A3 WO 2009154804A3 US 2009003729 W US2009003729 W US 2009003729W WO 2009154804 A3 WO2009154804 A3 WO 2009154804A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
peptide nucleic
twin
acid hybridization
hybridization probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/003729
Other languages
English (en)
Other versions
WO2009154804A2 (fr
Inventor
Eric Wickstrom
Nariman Amirkhanov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thomas Jefferson University
Original Assignee
Thomas Jefferson University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thomas Jefferson University filed Critical Thomas Jefferson University
Publication of WO2009154804A2 publication Critical patent/WO2009154804A2/fr
Publication of WO2009154804A3 publication Critical patent/WO2009154804A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0032—Methine dyes, e.g. cyanine dyes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0054—Macromolecular compounds, i.e. oligomers, polymers, dendrimers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0056—Peptides, proteins, polyamino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C07K14/003—Peptide-nucleic acids (PNAs)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un conjugué qui comprend une molécule d'acide nucléique peptidique, ladite molécule d'acide nucléique peptidique étant conjuguée à deux fluorophores identiques par le biais de lieurs peptidiques. La liaison du produit de recombinaison à une séquence d'ARN ou d'ADN complémentaire entraîne une augmentation de la fluorescence, qui permet de détecter l'événement de liaison. L'invention permet également d'identifier une séquence d'ARN ou d'ADN in vitro ou in vivo à l'aide du conjugué.
PCT/US2009/003729 2008-06-20 2009-06-22 Sondes d'hybridation d'acides nucléiques peptidiques à fluorophores jumeaux Ceased WO2009154804A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13260508P 2008-06-20 2008-06-20
US61/132,605 2008-06-20

Publications (2)

Publication Number Publication Date
WO2009154804A2 WO2009154804A2 (fr) 2009-12-23
WO2009154804A3 true WO2009154804A3 (fr) 2010-05-27

Family

ID=41434608

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/003729 Ceased WO2009154804A2 (fr) 2008-06-20 2009-06-22 Sondes d'hybridation d'acides nucléiques peptidiques à fluorophores jumeaux

Country Status (1)

Country Link
WO (1) WO2009154804A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108348622A (zh) * 2015-08-11 2018-07-31 耶路撒冷希伯来大学伊森姆研究发展有限公司 辐射发射肽核酸缀合物及其用于疾病、状况和紊乱的诊断、成像和治疗的用途
US11364259B2 (en) * 2016-12-27 2022-06-21 The University Of Tokyo MRNA functionalization method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005089524A2 (fr) * 2004-03-19 2005-09-29 U.S. Genomics, Inc. Compositions et procede de detection de molecules simples

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005089524A2 (fr) * 2004-03-19 2005-09-29 U.S. Genomics, Inc. Compositions et procede de detection de molecules simples

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
P.J. SCHUCK ET AL.: "A novel fluorophore for two-photon-excited single-molecule fluorescence", CHEMICAL PHYSICS, vol. 318, 2005, pages 7 - 11 *
XIAOBING TIAN ET AL.: "External Imaging of CCND1, MYC, and KRAS Oncogene mRNAs with Tumor-Targeted Radionuclide-PNA-Peptide Chimeras", ANNALS NEW YORK ACADEMY OF SCIENCE., vol. 1059, November 2005 (2005-11-01), pages 106 - 144 *
XIAOBING TIAN ET AL.: "Receptor-mediated internalization of chelator-PNA- peptide hybridization probes for radioimaging or magnetic resonance imaging of oncogene mRNAs in tumours", BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 35, February 2007 (2007-02-01), pages 72 - 76 *

Also Published As

Publication number Publication date
WO2009154804A2 (fr) 2009-12-23

Similar Documents

Publication Publication Date Title
EP2579899B1 (fr) Peptides pénétrant dans la cellule et leurs utilisations
WO2007070572A8 (fr) Sonde pour le sequencage d’acide nucleique et procedes d’utilisation
Hou et al. Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase
WO2009073345A3 (fr) Dosage pour détecter des anomalies génétiques dans des acides nucléiques génomiques
WO2007135368A3 (fr) Composés de type teinte et application de leurs conjugués marqués
WO2007123744A3 (fr) Systèmes et procédés pour analyse de séquençage par synthèse
WO2011001135A3 (fr) Diagnostic et traitement de la maladie d'alzheimer
WO2007136736A3 (fr) Procédés de tri et de synthèse d'acides nucléiques
WO2011034583A3 (fr) Inhibition de l'activation des récepteurs du type toll endosomaux
WO2010036359A3 (fr) Procédés de test d’activité de polymérase
WO2011029008A3 (fr) Corps de synthèse dirigés contre akt1
EP2004852A4 (fr) Amplification de fragments d'adn
Zhang et al. Targeting folded RNA: a branched peptide boronic acid that binds to a large surface area of HIV-1 RRE RNA
IL172297A0 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis iii
WO2009154804A3 (fr) Sondes d'hybridation d'acides nucléiques peptidiques à fluorophores jumeaux
EP2394665A1 (fr) Peptides pénétrant dans la cellule et leurs utilisations
Shtork et al. Diversifying i-motif-based pH sensors: labeling patterns tune the intracellular localization
EP2250182A4 (fr) Isolation de l'adn, de l'arn et de protéine à partir d'un seul et même échantillon
WO2005067648A3 (fr) Profilage spatial multiplex d'expression genique
WO2009058852A3 (fr) Ligand du petit sillon-oligonucléotides destinés au transfert d'énergie et procédés pour leur utilisation
WO2007086738A8 (fr) Acide atypique du gene de kinase semblable a la double courtine et son utilisation dans le diagnostic et la therapie de cancers d’origine neuroectodermique
WO2010129656A8 (fr) Procédé de marquage d'oligonucléotides à l'aide d'une réaction de cycloaddition
WO2006035273A3 (fr) Nouvelles sequences de nucleotides et d'acides amines, et leurs dosages et procedes d'utilisation pour le diagnostic
WO2007060671A3 (fr) Nouvelles sequences de nucleotides et d'acides amines, ainsi que tests et procedes d'utilisation de ces sequences a des fins de diagnostic
WO2025199140A1 (fr) Procédés et compositions associés à des balises à adn verrouillé pour la détection d'enzymes actives

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09767117

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09767117

Country of ref document: EP

Kind code of ref document: A2