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WO2009150477A3 - Transfer vehicle for producing proteins in transgenic animals - Google Patents

Transfer vehicle for producing proteins in transgenic animals Download PDF

Info

Publication number
WO2009150477A3
WO2009150477A3 PCT/GB2009/050677 GB2009050677W WO2009150477A3 WO 2009150477 A3 WO2009150477 A3 WO 2009150477A3 GB 2009050677 W GB2009050677 W GB 2009050677W WO 2009150477 A3 WO2009150477 A3 WO 2009150477A3
Authority
WO
WIPO (PCT)
Prior art keywords
transfer vehicle
vehicle
transgenic animals
producing proteins
site specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2009/050677
Other languages
French (fr)
Other versions
WO2009150477A2 (en
Inventor
Andreas Kolb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Aberdeen
Original Assignee
University of Aberdeen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Aberdeen filed Critical University of Aberdeen
Priority to EP09762026A priority Critical patent/EP2307550A2/en
Publication of WO2009150477A2 publication Critical patent/WO2009150477A2/en
Publication of WO2009150477A3 publication Critical patent/WO2009150477A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

There is provided a gene transfer vehicle comprising first and second site specific recombinase sites and at least one gene of interest located therebetween. The first and second site specific recombinase sites cannot recombine together. Optionally the vehicle encodes a recombinase enzyme. The gene transfer vehicle can be a viral vector, for example based on an adenovirus or retrovirus.The vehicle allows highly targeted insertion of a transgene enabling efficient insertion and expression.
PCT/GB2009/050677 2008-06-14 2009-06-15 Gene transfer vehicle and use therefor Ceased WO2009150477A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09762026A EP2307550A2 (en) 2008-06-14 2009-06-15 Transfer vehicle for producing proteins in transgenic animals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0810924.1 2008-06-14
GB0810924A GB0810924D0 (en) 2008-06-14 2008-06-14 In vivo transgenics

Publications (2)

Publication Number Publication Date
WO2009150477A2 WO2009150477A2 (en) 2009-12-17
WO2009150477A3 true WO2009150477A3 (en) 2010-02-11

Family

ID=39672298

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2009/050677 Ceased WO2009150477A2 (en) 2008-06-14 2009-06-15 Gene transfer vehicle and use therefor

Country Status (3)

Country Link
EP (1) EP2307550A2 (en)
GB (1) GB0810924D0 (en)
WO (1) WO2009150477A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018126095A1 (en) * 2016-12-29 2018-07-05 Applied Stemcell, Inc. Transgenic animals and method for bioproduction

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029284A2 (en) * 2002-09-30 2004-04-08 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029284A2 (en) * 2002-09-30 2004-04-08 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ELIZABETH A MAGA: "The Use of Recombinase Proteins to Generate Transgenic Large Animals", CLONING AND STEM CELLS, MARY ANN LIEBERT, LARCHMONT, US, vol. 3, no. 4, 1 January 2001 (2001-01-01), pages 233 - 241, XP007910656, ISSN: 1536-2302 *
HOUDEBINE LOUIS-MARIE: "The methods to generate transgenic animals and to control transgene expression.", JOURNAL OF BIOTECHNOLOGY 25 SEP 2002, vol. 98, no. 2-3, 25 September 2002 (2002-09-25), pages 145 - 160, XP002962237, ISSN: 0168-1656 *
IMPERIALE M J ET AL: "ADENOVIRUS VECTORS: BIOLOGY, DESIGN, AND PRODUCTION", CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY, SPRINGER, BERLIN, DE, vol. 273, 1 January 2004 (2004-01-01), pages 335 - 357, XP009070614, ISSN: 0070-217X *
KOLB ANDREAS F: "Genome engineering using site-specific recombinases.", CLONING AND STEM CELLS 2002, vol. 4, no. 1, 2002, pages 65 - 80, XP007910661, ISSN: 1536-2302 *
RYDING A D S ET AL: "Conditional transgenic technologies", JOURNAL OF ENDOCRINOLOGY, BRISTOL, GB, vol. 171, no. 1, 1 October 2001 (2001-10-01), pages 1 - 14, XP002249421, ISSN: 0022-0795 *
See also references of EP2307550A2 *
SORRELL ET AL: "Targeted modification of mammalian genomes", BIOTECHNOLOGY ADVANCES, ELSEVIER PUBLISHING, BARKING, GB, vol. 23, no. 7-8, 1 November 2005 (2005-11-01), pages 431 - 469, XP005076498, ISSN: 0734-9750 *

Also Published As

Publication number Publication date
WO2009150477A2 (en) 2009-12-17
GB0810924D0 (en) 2008-07-23
EP2307550A2 (en) 2011-04-13

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