[go: up one dir, main page]

WO2009148885A3 - Hematoxylin staining method to address gradient staining - Google Patents

Hematoxylin staining method to address gradient staining Download PDF

Info

Publication number
WO2009148885A3
WO2009148885A3 PCT/US2009/045190 US2009045190W WO2009148885A3 WO 2009148885 A3 WO2009148885 A3 WO 2009148885A3 US 2009045190 W US2009045190 W US 2009045190W WO 2009148885 A3 WO2009148885 A3 WO 2009148885A3
Authority
WO
WIPO (PCT)
Prior art keywords
staining
hematoxylin
gradient
composition
hematoxylin stain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/045190
Other languages
French (fr)
Other versions
WO2009148885A2 (en
Inventor
Jonathan Beer
Kurt Reinhardt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Ventana Medical Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Publication of WO2009148885A2 publication Critical patent/WO2009148885A2/en
Publication of WO2009148885A3 publication Critical patent/WO2009148885A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention is directed to any process and/or composition that substantially reduces or prevents gradient hematoxylin staining of tissue samples. Without being bound by a theory of operation, one possible explanation for observed gradient staining results is the formation of a more reactive staining species produced as a result of a pH change that occurs during the staining process. For an automated system, one disclosed embodiment comprises dispensing a hematoxylin stain onto a tissue sample, and then dispensing a bluing composition to the tissue sample to form a combined hematoxylin stain-bluing composition. The hematoxylin stain-bluing composition is then mixed, such as by agitation, on the slide sufficiently to reduce or eliminate gradient staining. Other approaches also can be employed, either alone or in combination with thoroughly mixing the hematoxylin stain-bluing composition on the slide. For example, the method may comprise treating the sample with process solutions, such as wash solutions, having a pH at or about that of the hematoxylin stain, such as a pH of from about 1 to about 6, more typically from about 2 to about 3.
PCT/US2009/045190 2008-05-30 2009-05-26 Hematoxylin staining method to address gradient staining Ceased WO2009148885A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13044208P 2008-05-30 2008-05-30
US61/130,442 2008-05-30

Publications (2)

Publication Number Publication Date
WO2009148885A2 WO2009148885A2 (en) 2009-12-10
WO2009148885A3 true WO2009148885A3 (en) 2010-01-28

Family

ID=40942438

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/045190 Ceased WO2009148885A2 (en) 2008-05-30 2009-05-26 Hematoxylin staining method to address gradient staining

Country Status (1)

Country Link
WO (1) WO2009148885A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108195653A (en) * 2017-09-16 2018-06-22 南京福怡科技发展股份有限公司 One kind redyes staining kit and preparation and application

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2839542A1 (en) 2011-06-17 2012-12-20 Constitution Medical, Inc. Fixative and staining solutions
EP2786114A4 (en) * 2011-11-30 2015-08-26 Dako Denmark As Method and system for automated deparaffinization and non-immunohistochemical special staining of tissue samples
EP2807490B1 (en) * 2012-01-26 2017-09-13 Leica Biosystems Richmond, Inc. Method for hematoxylin and eosin staining
CN103528872A (en) * 2013-10-14 2014-01-22 中国农业大学 Fluorescent staining technique of plant root tissue living body section and application of technique
AU2014363678B2 (en) * 2013-12-13 2016-12-08 Ventana Medical Systems, Inc. Staining reagents and other liquids for histological processing of biological specimens and associated technology
CN107110750A (en) * 2014-12-18 2017-08-29 文塔纳医疗系统公司 Hematoxylin solution and preparation and application comprising chlorion and sulfate ion
CN110621974A (en) * 2017-05-10 2019-12-27 文塔纳医疗系统公司 Stabilized two-part hematoxylin solution with pH adjustment
CN108414328A (en) * 2018-04-04 2018-08-17 南京烁朴生物科技有限公司 A kind of modified form hematoxylin dyeing liquor reagent, preparation method and colouring method
CN110823664A (en) * 2019-11-27 2020-02-21 武汉医尔特科技有限公司 Hematoxylin staining solution and preparation method thereof
WO2021183719A1 (en) * 2020-03-12 2021-09-16 Bowling Green State University Drug detection using cobalt thiocyanate and eosin y
EP4147024A2 (en) * 2020-05-08 2023-03-15 Ventana Medical Systems, Inc. Semi-synthesis and use of racemic hematoxylin
CN114279795B (en) * 2020-09-28 2024-06-25 天士力生物医药产业集团有限公司 Tissue sample rapid detection system, detection method and application
CN113790945B (en) * 2021-09-23 2024-02-20 南昌雨露实验器材有限公司 Improved hematoxylin dye solution and preparation method thereof
CN113945440A (en) * 2021-10-14 2022-01-18 山东高创医疗器械国家研究院有限公司 A kind of Pasteur staining kit and its preparation method and staining method
CN115014909A (en) * 2022-06-13 2022-09-06 四川金域医学检验中心有限公司 Efficient staining process method suitable for pathological tissues
CN117054202B (en) * 2023-08-15 2025-03-21 福州迈新生物技术开发有限公司 A hematoxylin staining solution suitable for fully automatic immunohistochemical staining instrument and a preparation method thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2916416A1 (en) * 1979-04-23 1980-11-06 Karl Hans Dr Heinlein POWDERED DETERGENT OR PREWASH
WO1992019952A1 (en) * 1991-05-07 1992-11-12 Wescor, Inc. Improved staining method for histology and cytology specimens
US5354366A (en) * 1993-09-27 1994-10-11 Deluxe Corporation Ink composition and resins and methods relating thereto
US5972230A (en) * 1997-08-28 1999-10-26 Kaiser Aluminum And Chemical Corporation Method for rejuvenation of filter elements used in the filtration of metalworking fluids
US20040191854A1 (en) * 2003-03-31 2004-09-30 Cytyc Corporation Papanicolau staining process
US20050150836A1 (en) * 2002-09-25 2005-07-14 Williams Charles S. Regeneration of chemically treated zeolite
US20050250211A1 (en) * 2002-04-15 2005-11-10 Kurt Reinhardt Automated high volume slide processing system

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2916416A1 (en) * 1979-04-23 1980-11-06 Karl Hans Dr Heinlein POWDERED DETERGENT OR PREWASH
WO1992019952A1 (en) * 1991-05-07 1992-11-12 Wescor, Inc. Improved staining method for histology and cytology specimens
US5354366A (en) * 1993-09-27 1994-10-11 Deluxe Corporation Ink composition and resins and methods relating thereto
US5972230A (en) * 1997-08-28 1999-10-26 Kaiser Aluminum And Chemical Corporation Method for rejuvenation of filter elements used in the filtration of metalworking fluids
US20050250211A1 (en) * 2002-04-15 2005-11-10 Kurt Reinhardt Automated high volume slide processing system
US20050150836A1 (en) * 2002-09-25 2005-07-14 Williams Charles S. Regeneration of chemically treated zeolite
US20040191854A1 (en) * 2003-03-31 2004-09-30 Cytyc Corporation Papanicolau staining process

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108195653A (en) * 2017-09-16 2018-06-22 南京福怡科技发展股份有限公司 One kind redyes staining kit and preparation and application

Also Published As

Publication number Publication date
WO2009148885A2 (en) 2009-12-10

Similar Documents

Publication Publication Date Title
WO2009148885A3 (en) Hematoxylin staining method to address gradient staining
CN104471405B (en) Methods and compositions for hematoxylin-eosin staining
WO2007084429A3 (en) Biological sample processing composition and method
MX2009003070A (en) Novel pectate lyase and method of use for bio-scouring.
Simmons et al. Water-soaked porous evidence: a comparison of processing methods
Chagas et al. The challenging SO2-mediated chemical build-up of protein aggregates in wines
WO2007106837A3 (en) Method for combining immunostaining and fish using covalently binding fluorophores
PT2162476E (en) Method for preparing an aqueous formulation containing an acrylic comb polymer solution and an acrylic thickening emulsion, formulation thus obtained and use thereof in paper coating
Truss et al. Quantitative surface analysis of hemp fibers using XPS, conventional and low voltage in‐lens SEM
TR201910282T4 (en) New method for coloring and processing substrates.
Yuan et al. Dyeing behaviors of ionic liquid treated wool
ATE486119T1 (en) COMPOSITION OF A WINDOW WASHING LIQUID, ADDITIVE CONCENTRATE FOR USE THEREIN AND METHOD FOR USE THEREOF
BRPI0709361A8 (en) composition, methods for preparing a food and method for treating listeria
ATE490369T1 (en) METHOD FOR HYDROPHOBIZING TEXTILE MATERIALS
US7399366B1 (en) Product and processes for preventing the occurrence of rust stains resulting from irrigation systems using water containing iron ions and for cleaning off rust stains resulting from using said irrigation systems
WO2010048212A3 (en) Methods and compositions for preventing artifacts in tissue samples
WO2011038022A3 (en) Methods and compositions for selectively treating skin
CA2941801A1 (en) Hematoxylin precipitate cleaning method and system
NL2024455B1 (en) Zeolite composition suitable for tanning leather
TW200700721A (en) Process for the determination and control of deposit formation in an aqueous system
CN102268669A (en) WC-TiC-Co cemented carbide metallographic staining agent and treatment method
Hastrup et al. Non-enzymatic depolymerization of cotton cellulose by fungal mimicking metabolites
Shalaby et al. Antimicrobial finishing of regular and modified nylon‐6 fabrics
TW200734624A (en) Method for quantitative determination of iron
Grigsby et al. Determination of resin distribution and coverage in MDF by fiber staining

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09759046

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09759046

Country of ref document: EP

Kind code of ref document: A2