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WO2009034186A3 - Process for cell cultivation - Google Patents

Process for cell cultivation Download PDF

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Publication number
WO2009034186A3
WO2009034186A3 PCT/EP2008/062212 EP2008062212W WO2009034186A3 WO 2009034186 A3 WO2009034186 A3 WO 2009034186A3 EP 2008062212 W EP2008062212 W EP 2008062212W WO 2009034186 A3 WO2009034186 A3 WO 2009034186A3
Authority
WO
WIPO (PCT)
Prior art keywords
bio
reactor
cell culture
inner surfaces
provides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2008/062212
Other languages
French (fr)
Other versions
WO2009034186A2 (en
Inventor
Nadia Zghoul
Kurt Dittmar
Werner Lindenmaier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Original Assignee
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum fuer Infektionsforschung HZI GmbH filed Critical Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Publication of WO2009034186A2 publication Critical patent/WO2009034186A2/en
Publication of WO2009034186A3 publication Critical patent/WO2009034186A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C12N2533/18—Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The present invention provides a process for the production of a structurally stable porous matrix material, the inner surfaces of which are at least partially colonized by adhering cells, the process involving the cultivation of mammalian cells under cell culture conditions in cell culture medium in the bio-reactor, which is agitated by a rocking movement. Accordingly, the bio-reactor used in the process of the invention is not provided with movable stirrers or static mixing elements, but rather provides essentially flat or curved inner surfaces, i.e. without elements projecting or protruding over the inner surface of the bio-reactor.
PCT/EP2008/062212 2007-09-13 2008-09-12 Process for cell cultivation Ceased WO2009034186A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07116301.8 2007-09-13
EP07116301 2007-09-13

Publications (2)

Publication Number Publication Date
WO2009034186A2 WO2009034186A2 (en) 2009-03-19
WO2009034186A3 true WO2009034186A3 (en) 2010-03-11

Family

ID=40452607

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/062212 Ceased WO2009034186A2 (en) 2007-09-13 2008-09-12 Process for cell cultivation

Country Status (1)

Country Link
WO (1) WO2009034186A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8399245B2 (en) 2009-02-18 2013-03-19 Terumo Bct, Inc. Rotation system for cell growth chamber of a cell expansion system and method of use therefor
US9057045B2 (en) 2009-12-29 2015-06-16 Terumo Bct, Inc. Method of loading and distributing cells in a bioreactor of a cell expansion system
US9518249B2 (en) 2010-12-16 2016-12-13 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9453197B2 (en) 2010-12-16 2016-09-27 General Electric Company Methods of making cell carrier
US9534206B2 (en) 2010-12-16 2017-01-03 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9926523B2 (en) 2010-12-16 2018-03-27 General Electric Company Cell carriers and methods for culturing cells
US9453196B2 (en) 2010-12-16 2016-09-27 General Electric Company Cell carrier, methods of making and use
US20120323325A1 (en) * 2011-06-16 2012-12-20 Fulton Judith A Autologous in situ tissue engineering
CA2889022A1 (en) * 2012-10-23 2014-05-01 Genzyme Corporation Perfusion culturing methods and uses thereof
MX369522B (en) 2013-02-22 2019-11-11 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof.
SG11201506343QA (en) 2013-02-22 2015-09-29 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof
WO2015073918A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
US11008547B2 (en) 2014-03-25 2021-05-18 Terumo Bct, Inc. Passive replacement of media
TWI743024B (en) 2014-06-06 2021-10-21 美商健臻公司 Perfusion culturing methods and uses thereof
TWI776235B (en) 2014-06-09 2022-09-01 美商健臻公司 Seed train processes and uses thereof
AU2015369809B2 (en) 2014-12-22 2022-03-17 Genzyme Corporation Methods of culturing a mammalian cell
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
CN112877210B (en) * 2021-02-25 2022-07-26 上海揽微赛尔生物科技有限公司 Stem cell culture perfusion device with controllable pH value
US20250188399A1 (en) * 2022-02-08 2025-06-12 Ivy Farm Technologies Limited Bioreactor

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH037575A (en) * 1989-03-30 1991-01-14 Shimadzu Corp Apparatus for cell culture
US5026650A (en) * 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
DE4229325A1 (en) * 1992-09-02 1994-03-03 Heraeus Sepatech Culture vessel for cell cultures
US5380662A (en) * 1990-06-01 1995-01-10 Robbins Scientific Corporation Hybridization incubator with rotisserie mechanism
US5492826A (en) * 1993-12-10 1996-02-20 William Beaumont Hospital Apparatus and method for seeding endothelial cells
US5686301A (en) * 1993-09-02 1997-11-11 Heraeus Instruments Gmbh Culture vessel for cell cultures
DE19915610A1 (en) * 1999-04-07 2000-10-19 Augustinus Bader Process for colonization of substrates with biological cells and colonization devices that can be used for this
EP1260580A1 (en) * 2001-05-21 2002-11-27 Core Biotech Co., Ltd. Cell culture tube and multiple roller tube cell culture system using the same
US20030036192A1 (en) * 2001-02-15 2003-02-20 Vijay Singh Disposable perfusion bioreactor for cell culture
WO2007045619A1 (en) * 2005-10-17 2007-04-26 Hannover Med Hochschule Tissue implant and process for its production

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5026650A (en) * 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
JPH037575A (en) * 1989-03-30 1991-01-14 Shimadzu Corp Apparatus for cell culture
US5380662A (en) * 1990-06-01 1995-01-10 Robbins Scientific Corporation Hybridization incubator with rotisserie mechanism
DE4229325A1 (en) * 1992-09-02 1994-03-03 Heraeus Sepatech Culture vessel for cell cultures
US5686301A (en) * 1993-09-02 1997-11-11 Heraeus Instruments Gmbh Culture vessel for cell cultures
US5492826A (en) * 1993-12-10 1996-02-20 William Beaumont Hospital Apparatus and method for seeding endothelial cells
DE19915610A1 (en) * 1999-04-07 2000-10-19 Augustinus Bader Process for colonization of substrates with biological cells and colonization devices that can be used for this
US20030036192A1 (en) * 2001-02-15 2003-02-20 Vijay Singh Disposable perfusion bioreactor for cell culture
EP1260580A1 (en) * 2001-05-21 2002-11-27 Core Biotech Co., Ltd. Cell culture tube and multiple roller tube cell culture system using the same
WO2007045619A1 (en) * 2005-10-17 2007-04-26 Hannover Med Hochschule Tissue implant and process for its production

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
C. SCHWARZ, V. KLEIN ; CHAMBERS HARRAP: "Concise Dictionary", 1999, CHAMBERS HARRAP, EDINGBURGH, XP002562803, 48971 *

Also Published As

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