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WO2009028662A1 - 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出 - Google Patents

細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出 Download PDF

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Publication number
WO2009028662A1
WO2009028662A1 PCT/JP2008/065532 JP2008065532W WO2009028662A1 WO 2009028662 A1 WO2009028662 A1 WO 2009028662A1 JP 2008065532 W JP2008065532 W JP 2008065532W WO 2009028662 A1 WO2009028662 A1 WO 2009028662A1
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WO
WIPO (PCT)
Prior art keywords
gene
cdt
cytolethal distending
distending toxin
bacterium belonging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/065532
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English (en)
French (fr)
Inventor
Shinji Yamasaki
Worada Samosorunsuk
Masahiro Asakura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fuso Pharmaceutical Industries Ltd
Osaka Metropolitan University
Original Assignee
Fuso Pharmaceutical Industries Ltd
Osaka Prefecture University PUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuso Pharmaceutical Industries Ltd, Osaka Prefecture University PUC filed Critical Fuso Pharmaceutical Industries Ltd
Priority to EP08828171.2A priority Critical patent/EP2192184B8/en
Priority to US12/675,726 priority patent/US8586327B2/en
Priority to CN200880113260.2A priority patent/CN101835897B/zh
Priority to AU2008292375A priority patent/AU2008292375B8/en
Priority to JP2009530205A priority patent/JP5345936B2/ja
Priority to EP17180547.6A priority patent/EP3255148B1/en
Priority to CA2697480A priority patent/CA2697480C/en
Publication of WO2009028662A1 publication Critical patent/WO2009028662A1/ja
Anticipated expiration legal-status Critical
Priority to US14/052,434 priority patent/US9663828B2/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/205—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Campylobacter (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C07K16/121—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Helicobacter (Campylobacter) (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56922—Campylobacter
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/205—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Campylobacter (G)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　本発明は、C. ハイオインテスティナリスの細胞膨張化致死毒CDTおよびそれをコードするポリヌクレオチドの提供、およびcdt遺伝子を利用したC. ハイオインテスティナリスの新規検出方法の提供を課題とする。 　発明者らは、カンピロバクター属細菌の細胞膨張化致死毒（CDT）に着目し、C. ジェジュニ、C. コリとC. フィータスのcdtA、cdtB およびcdtC 遺伝子を特異的に検出できるマルチプレックスPCR 法では増幅されないが、これら３菌種共通プライマーでcdtB遺伝子が増幅される菌株を、タイの腸炎患者から分離したカンピロバクター属様細菌のcdt遺伝子を検出することにより見出した。16S rRNA 遺伝子解析から、この菌株をC. ハイオインテスティナリスと同定し、さらに、cdtB遺伝子の上流、下流についてゲノムウォーキングにより、cdt遺伝子の全塩基配列を決定した。
PCT/JP2008/065532 2007-08-31 2008-08-29 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出 Ceased WO2009028662A1 (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP08828171.2A EP2192184B8 (en) 2007-08-31 2008-08-29 Detection of bacterium belonging to genus campylobacter which targets cytolethal distending toxin
US12/675,726 US8586327B2 (en) 2007-08-31 2008-08-29 Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin
CN200880113260.2A CN101835897B (zh) 2007-08-31 2008-08-29 通过靶向细胞致死肿胀毒素对弯曲杆菌属细菌进行的检测
AU2008292375A AU2008292375B8 (en) 2007-08-31 2008-08-29 Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin
JP2009530205A JP5345936B2 (ja) 2007-08-31 2008-08-29 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出
EP17180547.6A EP3255148B1 (en) 2007-08-31 2008-08-29 Detection of bacterium belonging to the genus campylobacter which targets cytolethal distending toxin
CA2697480A CA2697480C (en) 2007-08-31 2008-08-29 Detection of bacteria belonging to the genus campylobacter by targeting cytolethal distending toxin
US14/052,434 US9663828B2 (en) 2007-08-31 2013-10-11 Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007226013 2007-08-31
JP2007-226013 2007-08-31

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/675,726 A-371-Of-International US8586327B2 (en) 2007-08-31 2008-08-29 Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin
US14/052,434 Division US9663828B2 (en) 2007-08-31 2013-10-11 Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin

Publications (1)

Publication Number Publication Date
WO2009028662A1 true WO2009028662A1 (ja) 2009-03-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/065532 Ceased WO2009028662A1 (ja) 2007-08-31 2008-08-29 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出

Country Status (8)

Country Link
US (2) US8586327B2 (ja)
EP (2) EP3255148B1 (ja)
JP (2) JP5345936B2 (ja)
KR (1) KR101588065B1 (ja)
CN (1) CN101835897B (ja)
AU (1) AU2008292375B8 (ja)
CA (1) CA2697480C (ja)
WO (1) WO2009028662A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013255507A (ja) * 2007-08-31 2013-12-26 Osaka Prefecture Univ 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7563594B2 (en) 2003-12-05 2009-07-21 Fuso Pharmaceutical Industries, Ltd. Cytolethal distending toxins and detection of Campylobacter bacteria using the same as a target
CN106801052B (zh) * 2017-01-19 2019-07-16 广东省农业科学院动物卫生研究所 一种猪等孢球虫rRNA基因序列及其获取方法
WO2019004804A2 (ko) * 2017-06-30 2019-01-03 한국화학연구원 패혈증 진단용 키트 및 이를 이용한 진단방법
CN112899379B (zh) * 2020-12-30 2022-05-20 广东省微生物研究所（广东省微生物分析检测中心） 含有特异性分子靶标的空肠弯曲菌标准菌株及其检测和应用

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013255507A (ja) * 2007-08-31 2013-12-26 Osaka Prefecture Univ 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出
US9663828B2 (en) 2007-08-31 2017-05-30 Osaka Prefecture University Public Corporation Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin

Also Published As

Publication number Publication date
JP2013255507A (ja) 2013-12-26
KR20100080595A (ko) 2010-07-09
JP5770795B2 (ja) 2015-08-26
EP3255148A1 (en) 2017-12-13
KR101588065B1 (ko) 2016-01-22
EP3255148B1 (en) 2019-06-19
US20100273161A1 (en) 2010-10-28
EP2192184A4 (en) 2011-08-17
US20150184230A1 (en) 2015-07-02
AU2008292375B8 (en) 2014-08-14
AU2008292375A1 (en) 2009-03-05
AU2008292375B2 (en) 2014-07-03
CA2697480A1 (en) 2009-03-05
EP2192184B1 (en) 2017-08-09
JPWO2009028662A1 (ja) 2010-12-02
CA2697480C (en) 2017-07-11
AU2008292375A2 (en) 2010-04-29
EP2192184B8 (en) 2017-11-08
CN101835897B (zh) 2014-05-14
JP5345936B2 (ja) 2013-11-20
EP2192184A1 (en) 2010-06-02
US8586327B2 (en) 2013-11-19
US9663828B2 (en) 2017-05-30
CN101835897A (zh) 2010-09-15

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