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WO2009027507A3 - Aptamères se liant à une molécule cible intervenant dans l'hémostase - Google Patents

Aptamères se liant à une molécule cible intervenant dans l'hémostase Download PDF

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Publication number
WO2009027507A3
WO2009027507A3 PCT/EP2008/061395 EP2008061395W WO2009027507A3 WO 2009027507 A3 WO2009027507 A3 WO 2009027507A3 EP 2008061395 W EP2008061395 W EP 2008061395W WO 2009027507 A3 WO2009027507 A3 WO 2009027507A3
Authority
WO
WIPO (PCT)
Prior art keywords
aptamer
aptamers
bind
haemostasis
target molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2008/061395
Other languages
German (de)
English (en)
Other versions
WO2009027507A2 (fr
Inventor
Guenter Mayer
Jens Mueller
Bernd Poetzsch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rheinische Friedrich Wilhelms Universitaet Bonn
Original Assignee
Rheinische Friedrich Wilhelms Universitaet Bonn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rheinische Friedrich Wilhelms Universitaet Bonn filed Critical Rheinische Friedrich Wilhelms Universitaet Bonn
Publication of WO2009027507A2 publication Critical patent/WO2009027507A2/fr
Publication of WO2009027507A3 publication Critical patent/WO2009027507A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un aptamère se liant à une molécule cible intervenant dans l'hémostase, l'aptamère étant un aptamère se liant à la protéine C activée, lequel aptamère se lie à la protéine C activée avec une constante de dissociation KD comprise entre ≥ 0,001 nM et ≤ 80 nM et présente une longueur comprise entre ≥ 20 nucléotides et ≤ 160 nucléotides, ou ledit aptamère étant un aptamère de fusion se liant à la thrombine, qui comprend au moins deux aptamères se liant à la thrombine et au moins un lieur, ledit au moins un lieur liant lesdits au moins deux aptamères se liant à la thrombine.
PCT/EP2008/061395 2007-08-31 2008-08-29 Aptamères se liant à une molécule cible intervenant dans l'hémostase Ceased WO2009027507A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007041476.7A DE102007041476B4 (de) 2007-08-31 2007-08-31 Aptamere, die an ein an der Hämostase beteiligtes Zielmolekül binden
DE102007041476.7 2007-08-31

Publications (2)

Publication Number Publication Date
WO2009027507A2 WO2009027507A2 (fr) 2009-03-05
WO2009027507A3 true WO2009027507A3 (fr) 2009-05-07

Family

ID=40120244

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/061395 Ceased WO2009027507A2 (fr) 2007-08-31 2008-08-29 Aptamères se liant à une molécule cible intervenant dans l'hémostase

Country Status (2)

Country Link
DE (1) DE102007041476B4 (fr)
WO (1) WO2009027507A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013042077A1 (fr) * 2011-09-23 2013-03-28 Csir Diagnostic de tuberculose
US9545383B2 (en) 2014-04-01 2017-01-17 Massachusetts Institute Of Technology Blood clotting control
EP2980218A1 (fr) * 2014-07-30 2016-02-03 Rheinische Friedrich-Wilhelms-Universität Bonn Complexe de thrombine aptamère à utiliser comme antidote à action directe sur les inhibiteurs de la thrombine
CN111440235B (zh) * 2020-04-16 2022-11-25 成都中医药大学 一种捕获水蛭素类多肽的探针及其应用
WO2021245586A2 (fr) * 2020-06-03 2021-12-09 Rhodes University Aptamères de liaison à cd4 et leurs applications

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
DK0786469T3 (da) 1990-06-11 2006-07-10 Gilead Sciences Inc Nukleinsyreligander
US5650275A (en) 1990-06-11 1997-07-22 Nexstar Pharmacueticals Inc Target detection method using spectroscopically detectable nucleic acid ligands
EP0572529A4 (fr) * 1991-02-21 1994-11-02 Gilead Sciences Inc Aptamere specifique de biomolecules et procede de production.
DE60141844D1 (de) 2000-10-02 2010-05-27 Oklahoma Med Res Found Assay zum schnellen nachweis vom humanen aktivierten protein c und hochspezifischer monoklonaler antikörper dagegen
AUPR604101A0 (en) 2001-06-29 2001-07-26 Unisearch Limited Aptamers
WO2005059509A2 (fr) 2003-12-12 2005-06-30 Saint Louis University Biocapteurs permettant de detecter des macromolecules et d'autres analytes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BOCK L C ET AL: "SELECTION OF SINGLE-STRANDED DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN", NATURE, NATURE PUBLISHING GROUP, LONDON, UK, vol. 355, no. 6360, 6 February 1992 (1992-02-06), pages 564 - 566, XP000453533, ISSN: 0028-0836 *
FAMULOK MICHAEL ET AL: "Functional aptamers and aptazymes in biotechnology, diagnostics, and therapy.", CHEMICAL REVIEWS SEP 2007, vol. 107, no. 9, 23 August 2007 (2007-08-23), pages 3715 - 3743, XP002508782, ISSN: 0009-2665 *
GAL SANG WAN ET AL: "Selection of a RNA aptamer that binds to human activated protein C and inhibits its protease function", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 252, no. 3, March 1998 (1998-03-01), pages 553 - 562, XP002508781, ISSN: 0014-2956 *
TASSET D M ET AL: "Oligonucleotide inhibitors of human thrombin that bind distinct epitopes", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 272, no. 5, 10 October 1997 (1997-10-10), pages 688 - 698, XP004453692, ISSN: 0022-2836 *

Also Published As

Publication number Publication date
DE102007041476A1 (de) 2009-04-09
DE102007041476B4 (de) 2017-03-30
WO2009027507A2 (fr) 2009-03-05

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