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WO2009022682A1 - 標的物質の検出方法 - Google Patents

標的物質の検出方法 Download PDF

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Publication number
WO2009022682A1
WO2009022682A1 PCT/JP2008/064422 JP2008064422W WO2009022682A1 WO 2009022682 A1 WO2009022682 A1 WO 2009022682A1 JP 2008064422 W JP2008064422 W JP 2008064422W WO 2009022682 A1 WO2009022682 A1 WO 2009022682A1
Authority
WO
WIPO (PCT)
Prior art keywords
dimer
region
probe
probes
forming
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/064422
Other languages
English (en)
French (fr)
Inventor
Mitsugu Usui
Chikako Hakii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eisai R&D Management Co Ltd
Original Assignee
Eisai R&D Management Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai R&D Management Co Ltd filed Critical Eisai R&D Management Co Ltd
Priority to US12/733,164 priority Critical patent/US8450058B2/en
Priority to EP08827494A priority patent/EP2180063B1/en
Priority to JP2009528128A priority patent/JP5289314B2/ja
Publication of WO2009022682A1 publication Critical patent/WO2009022682A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　パルサー法における検出感度の向上と多遺伝子同時検出が可能な標的物質の検出方法及び検出用キットを提供する。 　複数のダイマープローブ又はダイマー形成用プローブと、アシストプローブとを用いてシグナルプローブポリマーを形成させて標的物質を検出する方法であって、各ダイマープローブは５’側領域、中央領域及び３’側領域を含む、２種のダイマー形成用プローブ（２種のダイマー形成用プローブは中央領域が互いに相補的であり且つ３’側領域及び５’側領域が互いに非相補的である）から形成され、複数のダイマープローブは自己集合反応によりダイマープローブのポリマーを形成し得るように構成されており、アシストプローブは１つのダイマープローブの一方のダイマー形成用プローブの５’側領域に相補的な領域、他方のダイマー形成用プローブの３’側領域に相補的な領域、及び標的物質に結合可能なターゲット領域を有するようにした。
PCT/JP2008/064422 2007-08-14 2008-08-11 標的物質の検出方法 Ceased WO2009022682A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/733,164 US8450058B2 (en) 2007-08-14 2008-08-11 Method of detecting target substance
EP08827494A EP2180063B1 (en) 2007-08-14 2008-08-11 Method of detecting target substance
JP2009528128A JP5289314B2 (ja) 2007-08-14 2008-08-11 標的物質の検出方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-211521 2007-08-14
JP2007211521 2007-08-14

Publications (1)

Publication Number Publication Date
WO2009022682A1 true WO2009022682A1 (ja) 2009-02-19

Family

ID=40350734

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/064422 Ceased WO2009022682A1 (ja) 2007-08-14 2008-08-11 標的物質の検出方法

Country Status (4)

Country Link
US (1) US8450058B2 (ja)
EP (1) EP2180063B1 (ja)
JP (1) JP5289314B2 (ja)
WO (1) WO2009022682A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2023204045A1 (ja) * 2022-04-20 2023-10-26

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002031192A1 (en) 2000-10-11 2002-04-18 Sanko Junyaku Co., Ltd. Method of constructing self-assembly of probes and method of detecting the same
WO2006028162A1 (ja) 2004-09-08 2006-03-16 Eisai R & D Management Co., Ltd. シグナルプローブポリマーの形成方法
WO2006093097A1 (ja) * 2005-02-28 2006-09-08 Eisai R&D Management Co., Ltd. アシストプローブ及びその利用方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2039517C (en) * 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
KR20040041529A (ko) * 2001-09-28 2004-05-17 산코 준야쿠 가부시키가이샤 Ｄｎａ 칩의 시그널 증폭방법
CA2515734C (en) * 2003-02-14 2013-04-09 Eisai Co., Ltd. Signal amplification method for detecting expressed gene
CA2516360C (en) * 2003-02-21 2012-07-10 Eisai Co. Ltd. Signal amplification method for detecting mutant gene
KR101146251B1 (ko) * 2004-04-28 2012-05-16 에자이 알앤드디 매니지먼트 가부시키가이샤 혼성화 방법
US7862998B2 (en) * 2005-02-28 2011-01-04 Eisai R&D Management Co., Ltd. Assist probe and method of using the same
WO2007037282A1 (ja) * 2005-09-27 2007-04-05 Eisai R & D Management Co., Ltd. 微小粒子に自己集合体を形成させる方法及び標的分析物の検出方法
WO2009022683A1 (ja) * 2007-08-14 2009-02-19 Eisai R & D Management Co., Ltd. 標的物質の検出方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002031192A1 (en) 2000-10-11 2002-04-18 Sanko Junyaku Co., Ltd. Method of constructing self-assembly of probes and method of detecting the same
WO2006028162A1 (ja) 2004-09-08 2006-03-16 Eisai R & D Management Co., Ltd. シグナルプローブポリマーの形成方法
WO2006093097A1 (ja) * 2005-02-28 2006-09-08 Eisai R&D Management Co., Ltd. アシストプローブ及びその利用方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2023204045A1 (ja) * 2022-04-20 2023-10-26
WO2023204045A1 (ja) * 2022-04-20 2023-10-26 積水メディカル株式会社 超高感度アナライト測定方法
JP7529352B2 (ja) 2022-04-20 2024-08-06 積水メディカル株式会社 超高感度アナライト測定方法

Also Published As

Publication number Publication date
JPWO2009022682A1 (ja) 2010-11-18
EP2180063A1 (en) 2010-04-28
EP2180063A4 (en) 2011-01-19
EP2180063B1 (en) 2013-01-09
US20100151592A1 (en) 2010-06-17
US8450058B2 (en) 2013-05-28
JP5289314B2 (ja) 2013-09-11

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