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WO2009015367A3 - Stabilizing proteins using ionic liquids - Google Patents

Stabilizing proteins using ionic liquids Download PDF

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Publication number
WO2009015367A3
WO2009015367A3 PCT/US2008/071254 US2008071254W WO2009015367A3 WO 2009015367 A3 WO2009015367 A3 WO 2009015367A3 US 2008071254 W US2008071254 W US 2008071254W WO 2009015367 A3 WO2009015367 A3 WO 2009015367A3
Authority
WO
WIPO (PCT)
Prior art keywords
ionic liquids
stabilizing proteins
stabilizing
compositions
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/071254
Other languages
French (fr)
Other versions
WO2009015367A2 (en
Inventor
C Austen Angell
Nolene Byrne
Jean-Philippe Belieres
Limin Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arizona State University ASU
Original Assignee
Arizona State University ASU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arizona State University ASU filed Critical Arizona State University ASU
Publication of WO2009015367A2 publication Critical patent/WO2009015367A2/en
Publication of WO2009015367A3 publication Critical patent/WO2009015367A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Among other things, techniques and compositions for stabilizing proteins are disclosed. To stabilizing a protein, lyophilized protein is dissolved in a low-viscous solvent that includes water and ionic liquid. Compositions and techniques relate to reversible folding-unfolding, aggregation protection and multi-year stabilization in high protein concentration solutions of protic and/or aprotic ionic liquids.
PCT/US2008/071254 2007-07-25 2008-07-25 Stabilizing proteins using ionic liquids Ceased WO2009015367A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95188607P 2007-07-25 2007-07-25
US60/951,886 2007-07-25

Publications (2)

Publication Number Publication Date
WO2009015367A2 WO2009015367A2 (en) 2009-01-29
WO2009015367A3 true WO2009015367A3 (en) 2009-06-25

Family

ID=40282177

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/071254 Ceased WO2009015367A2 (en) 2007-07-25 2008-07-25 Stabilizing proteins using ionic liquids

Country Status (1)

Country Link
WO (1) WO2009015367A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883146B2 (en) 2007-11-30 2014-11-11 Abbvie Inc. Protein formulations and methods of making same
WO2011069037A2 (en) 2009-12-03 2011-06-09 The University Of North Carolina At Charlotte Stabilization and storage of biological pharmaceutical compositions
US10497970B2 (en) 2013-03-14 2019-12-03 Arizona Board Of Regents On Behalf Of Arizona State University Alkali ion conducting plastic crystals
IL312865B2 (en) 2013-09-11 2025-06-01 Eagle Biologics Inc Liquid protein formulations containing viscosity-reducing agents
US11471479B2 (en) 2014-10-01 2022-10-18 Eagle Biologics, Inc. Polysaccharide and nucleic acid formulations containing viscosity-lowering agents
CN116286771B (en) * 2023-02-03 2024-02-23 上海碧云天生物技术股份有限公司 Broad-spectrum protein stable preservation solution, preparation method and application thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANGELL C. ET AL.: "Parallel Developments in Aprotic and Protic Ionic Liquids: Physical Chemistry and Applications", ACC. CHEM. RES., vol. 40, no. 11, 2007, pages 1228 - 1236 *
BYRNE N. ET AL.: "Protein Unfolding, and the ''Tuning In'' of Reversible Intermediate States, in Protic Ionic Liquid Media", JOURNAL OF MOLECULAR BIOLOGY., vol. 378, no. 3, May 2008 (2008-05-01), pages 707 - 714, XP022762546, DOI: doi:10.1016/j.jmb.2008.02.050 *
BYRNE N. ET AL.: "Reversible Folding-unfolding, Aggregation Protection, and Multi-year Stabilization, in High Concentration Protein Solutions, Using Ionic Liquids", CHEMICAL COMMUNICATIONS, vol. 26, 19 April 2007 (2007-04-19), pages 2714 - 2716 *
TAMAR L. ET AL.: "Many Protic Ionic Liquids Mediate Hydrocarbon-Solvent Interactions and Promote Amphiphile Self-Assembly", LANGMUIR., vol. 23, no. 2, 2007, pages 402 - 404 *

Also Published As

Publication number Publication date
WO2009015367A2 (en) 2009-01-29

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