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WO2009014117A1 - Ｌ－リジンの製造法 - Google Patents

Ｌ－リジンの製造法 Download PDF

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Publication number
WO2009014117A1
WO2009014117A1 PCT/JP2008/063126 JP2008063126W WO2009014117A1 WO 2009014117 A1 WO2009014117 A1 WO 2009014117A1 JP 2008063126 W JP2008063126 W JP 2008063126W WO 2009014117 A1 WO2009014117 A1 WO 2009014117A1
Authority
WO
WIPO (PCT)
Prior art keywords
lysine
culture medium
escherichia coli
coli cell
succinyldiaminopimelate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/063126
Other languages
English (en)
French (fr)
Inventor
Hidetaka Doi
Takuji Ueda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to ES08791407T priority Critical patent/ES2403199T3/es
Priority to PL08791407T priority patent/PL2180052T3/pl
Priority to BRPI0814659-4A priority patent/BRPI0814659B1/pt
Priority to EP08791407A priority patent/EP2180052B1/en
Priority to CN200880100226.1A priority patent/CN101765659B/zh
Publication of WO2009014117A1 publication Critical patent/WO2009014117A1/ja
Priority to US12/691,211 priority patent/US20100190217A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Ｌ－リジン生産能を有するエシェリヒア・コリであって、メソ－α,ε－ジアミノピメリン酸合成経路の１又は２以上の酵素、例えば２，３，４，５－テトラヒドロピリジン－２，６－ジカルボン酸　Ｎ－スクシニルトランスフェラーゼ、スクシニルジアミノピメリン酸トランスアミナーゼ、スクシニルジアミノピメリン酸デスクシニラーゼ、又はジアミノピメリン酸エピメラーゼの活性が低下するように改変され、かつ、ジアミノピメリン酸デヒドロゲナーゼをコードする遺伝子が導入されたエシェリヒア・コリを培地中で培養し、該培地からＬ－リジンを採取することにより、Ｌ－リジンを製造する。
PCT/JP2008/063126 2007-07-23 2008-07-22 Ｌ－リジンの製造法 Ceased WO2009014117A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ES08791407T ES2403199T3 (es) 2007-07-23 2008-07-22 Método para producir L-lisina
PL08791407T PL2180052T3 (pl) 2007-07-23 2008-07-22 Sposób wytwarzania L-lizyny
BRPI0814659-4A BRPI0814659B1 (pt) 2007-07-23 2008-07-22 Método para produzir l-lisina
EP08791407A EP2180052B1 (en) 2007-07-23 2008-07-22 Method for production of l-lysine
CN200880100226.1A CN101765659B (zh) 2007-07-23 2008-07-22 L-赖氨酸的生产方法
US12/691,211 US20100190217A1 (en) 2007-07-23 2010-01-21 Method for producing l-lysine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-190795 2007-07-23
JP2007190795A JP2010226956A (ja) 2007-07-23 2007-07-23 Ｌ−リジンの製造法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/691,211 Continuation US20100190217A1 (en) 2007-07-23 2010-01-21 Method for producing l-lysine

Publications (1)

Publication Number Publication Date
WO2009014117A1 true WO2009014117A1 (ja) 2009-01-29

Family

ID=40281368

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063126 Ceased WO2009014117A1 (ja) 2007-07-23 2008-07-22 Ｌ－リジンの製造法

Country Status (9)

Country Link
US (1) US20100190217A1 (ja)
EP (1) EP2180052B1 (ja)
JP (1) JP2010226956A (ja)
KR (1) KR101537508B1 (ja)
CN (1) CN101765659B (ja)
BR (1) BRPI0814659B1 (ja)
ES (1) ES2403199T3 (ja)
PL (1) PL2180052T3 (ja)
WO (1) WO2009014117A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108504617A (zh) * 2018-04-10 2018-09-07 江南大学 一种高产l-赖氨酸的大肠杆菌重组菌及其构建方法
CN116042416A (zh) * 2023-01-09 2023-05-02 天津科技大学 高产ε-聚赖氨酸的多基因过表达链霉菌工程菌株及方法与应用

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7915018B2 (en) * 2004-10-22 2011-03-29 Ajinomoto Co., Inc. Method for producing L-amino acids using bacteria of the Enterobacteriaceae family
EP2186881B1 (en) * 2006-03-23 2014-04-23 Ajinomoto Co., Inc. A method for producing an L-amino acid using bacterium of the Enterobacteriaceae family with attenuated expression of a gene coding for small RNA
JP2011167071A (ja) * 2008-05-22 2011-09-01 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2012223092A (ja) 2009-08-28 2012-11-15 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2013074795A (ja) 2010-02-08 2013-04-25 Ajinomoto Co Inc 変異型ｒｐｓＡ遺伝子及びＬ−アミノ酸の製造法
JP2014036576A (ja) 2010-12-10 2014-02-27 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
PE20141569A1 (es) 2011-11-11 2014-11-08 Ajinomoto Kk Metodo para producir una sustancia diana mediante fermentacion
KR101518860B1 (ko) * 2013-10-11 2015-05-12 씨제이제일제당 (주) L-아미노산의 생산 방법
JP2017131111A (ja) 2014-06-03 2017-08-03 味の素株式会社 Ｌ−アミノ酸の製造法
KR101793328B1 (ko) * 2015-07-03 2017-11-03 씨제이제일제당 (주) Ｌ-라이신 생산능을 갖는 미생물 및 이를 이용한 ｌ-라이신 생산 방법
CN106190997B (zh) * 2016-07-20 2024-09-17 清华大学 一种nadh依赖性的二氨基庚二酸脱氢酶及其应用
EP3625351A1 (en) * 2017-05-19 2020-03-25 Zymergen Inc. Genomic engineering of biosynthetic pathways leading to increased nadph
CN114008070A (zh) * 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
CN111411092B (zh) * 2020-03-08 2022-07-05 安徽丰原发酵技术工程研究有限公司 高产l-赖氨酸的谷氨酸棒状杆菌及其应用
CN113201536B (zh) 2020-08-19 2022-09-13 中国科学院天津工业生物技术研究所 具有启动子活性的多核苷酸及其在生产氨基酸中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11192088A (ja) * 1993-12-08 1999-07-21 Ajinomoto Co Inc 発酵法によるｌ−リジンの製造法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830716A (en) * 1993-10-28 1998-11-03 Ajinomoto Co., Inc. Increased amounts of substances by modifying a microorganism to increase production of NADPH from NADH
JP4075087B2 (ja) * 1996-12-05 2008-04-16 味の素株式会社 Ｌ−リジンの製造法
HU224055B1 (hu) * 1997-10-04 2005-05-30 Degussa Ag Eljárás aszparaginsav és/vagy glutaminsav családba tartozó aminosavak mikrobiális termeltetésére, és hatóanyagok az eljárásban történő alkalmazásra
JP4380029B2 (ja) * 2000-07-05 2009-12-09 味の素株式会社 微生物を利用した物質の製造法
US7049106B2 (en) * 2001-04-10 2006-05-23 Degussa Ag Process for the production of L-amino acids by fermentation using coryneform bacteria with an attenuated mqo gene
JP3732789B2 (ja) * 2002-02-15 2006-01-11 株式会社トプコン 眼特性測定装置用模型眼及びその校正方法
WO2005010794A2 (en) * 2003-07-29 2005-02-03 Ajinomoto Co., Inc. Method for determining metabolic flux affecting substance production
ES2331956T3 (es) * 2004-01-30 2010-01-21 Ajinomoto Co., Inc. Microorganismo que produce l-aminoacidos y procedimiento para producir l-aminoacidos.
US7915018B2 (en) * 2004-10-22 2011-03-29 Ajinomoto Co., Inc. Method for producing L-amino acids using bacteria of the Enterobacteriaceae family
US7547531B2 (en) * 2005-01-18 2009-06-16 Ajinomoto Co., Inc. L-amino acid producing microorganism which has been modified to inactive the fimH gene, and a method for producing I-amino acid
EP1929028A1 (en) * 2005-09-27 2008-06-11 Ajinomoto Co., Inc. An l-amino acid-producing bacterium and a method for producing l-amino acids
WO2007037460A1 (en) * 2005-09-27 2007-04-05 Ajinomoto Co., Inc. An l-amino acid-producing bacterium and a method for producing l-amino acids
EP2186881B1 (en) * 2006-03-23 2014-04-23 Ajinomoto Co., Inc. A method for producing an L-amino acid using bacterium of the Enterobacteriaceae family with attenuated expression of a gene coding for small RNA
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
US8298295B2 (en) * 2007-09-28 2012-10-30 Intel Corporation Theft-deterrence method and apparatus for processor based devices

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11192088A (ja) * 1993-12-08 1999-07-21 Ajinomoto Co Inc 発酵法によるｌ−リジンの製造法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ISHINO S. ET AL.: "Cloning and Sequencing of the meso-Diaminopimelate-D-dehydrogenase (ddh) Gene of Corynebacterium glutamicum", AGRIC. BIOL. CHEM., vol. 52, no. 11, 1988, pages 2903 - 2909, XP008099523 *
KIM O.M. ET AL.: "Cloning and Sequencing of the ddh Gene involved in the Novel Pathway of Lysine Biosynthesis from Brevibacterium lactofermentum", J. MICROBIOL. BIOTECHNOL., vol. 5, no. 5, 1995, pages 250 - 256, XP007913418 *
See also references of EP2180052A4 *
YEH P. ET AL.: "General organization of the genes specifically onvolved in the diaminopimelate-lysine biosynthetic pathway of Corynebacterium glutamicum", MOL. GEN. GENET., vol. 212, 1988, pages 105 - 111, XP008099352 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108504617A (zh) * 2018-04-10 2018-09-07 江南大学 一种高产l-赖氨酸的大肠杆菌重组菌及其构建方法
CN116042416A (zh) * 2023-01-09 2023-05-02 天津科技大学 高产ε-聚赖氨酸的多基因过表达链霉菌工程菌株及方法与应用

Also Published As

Publication number Publication date
CN101765659A (zh) 2010-06-30
CN101765659B (zh) 2014-09-24
EP2180052A4 (en) 2011-06-29
KR20100056460A (ko) 2010-05-27
KR101537508B1 (ko) 2015-07-17
EP2180052B1 (en) 2013-01-23
BRPI0814659B1 (pt) 2023-01-17
EP2180052A1 (en) 2010-04-28
US20100190217A1 (en) 2010-07-29
PL2180052T3 (pl) 2013-06-28
JP2010226956A (ja) 2010-10-14
BRPI0814659A2 (pt) 2014-10-21
ES2403199T3 (es) 2013-05-16

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