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WO2009013620A3 - Recombinaison homologue - Google Patents

Recombinaison homologue Download PDF

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Publication number
WO2009013620A3
WO2009013620A3 PCT/IB2008/002572 IB2008002572W WO2009013620A3 WO 2009013620 A3 WO2009013620 A3 WO 2009013620A3 IB 2008002572 W IB2008002572 W IB 2008002572W WO 2009013620 A3 WO2009013620 A3 WO 2009013620A3
Authority
WO
WIPO (PCT)
Prior art keywords
heavy chain
antibodies
functionality
human
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2008/002572
Other languages
English (en)
Other versions
WO2009013620A2 (fr
Inventor
Frank Grosveld
Rick Janssens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Erasmus University Medical Center
Original Assignee
Erasmus University Medical Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0711244A external-priority patent/GB0711244D0/en
Priority claimed from GB0805281A external-priority patent/GB0805281D0/en
Application filed by Erasmus University Medical Center filed Critical Erasmus University Medical Center
Publication of WO2009013620A2 publication Critical patent/WO2009013620A2/fr
Publication of WO2009013620A3 publication Critical patent/WO2009013620A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C07K16/462—Igs containing a variable region (Fv) from one specie and a constant region (Fc) from another
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention concerne des procédés permettant de produire des anticorps uniquement à chaîne lourde in vivo chez des mammifères non humains à partir de gènes d'immunoglobuline à chaîne lourde manquant de fonctionnalité CHI en réponse à un test de provocation antigénique. L'invention concerne également des anticorps à chaînes lourde humains uniquement et des domaines de liaison VH humains solubles générés par des procédés selon l'invention, en particulier. L'invention concerne également des mammifères non humains appropriés pour l'expression de divers locus de gène à chaîne lourde uniquement de vertébrés manquant de fonctionnalité CHI, et de mélanges comprenant des locus à chaîne lourde uniquement hybrides de segments de gènes V, D et J et des combinaisons de régions effectrices constantes manquant de fonctionnalité CHI émanant de diverses espèces de vertébrés appropriées pour la production d'anticorps à chaînes lourdes uniquement et de domaines VH solubles.
PCT/IB2008/002572 2007-06-11 2008-06-11 Recombinaison homologue Ceased WO2009013620A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0711244A GB0711244D0 (en) 2007-06-11 2007-06-11 Binding molecules
GB0711244.4 2007-06-11
GB0805281.3 2008-03-20
GB0805281A GB0805281D0 (en) 2008-03-20 2008-03-20 Homologous recombination

Publications (2)

Publication Number Publication Date
WO2009013620A2 WO2009013620A2 (fr) 2009-01-29
WO2009013620A3 true WO2009013620A3 (fr) 2009-05-14

Family

ID=40281904

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/002572 Ceased WO2009013620A2 (fr) 2007-06-11 2008-06-11 Recombinaison homologue

Country Status (1)

Country Link
WO (1) WO2009013620A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9434782B2 (en) 2009-07-08 2016-09-06 Kymab Limited Animal models and therapeutic molecules
US12486336B2 (en) 2015-09-30 2025-12-02 Igm Biosciences, Inc. Binding molecules with modified J-chain

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006008548A2 (fr) 2004-07-22 2006-01-26 Erasmus University Medical Centre Rotterdam Molecules de liaison
CA2747534C (fr) 2008-12-18 2020-08-18 Erasmus University Medical Center Rotterdam Animaux transgeniques non humains exprimant des anticorps humanises et leur utilisation
GB0905023D0 (en) 2009-03-24 2009-05-06 Univ Erasmus Medical Ct Binding molecules
US9445581B2 (en) 2012-03-28 2016-09-20 Kymab Limited Animal models and therapeutic molecules
ES2724975T3 (es) 2009-12-10 2019-09-18 Regeneron Pharma Ratones que producen anticuerpos de cadena pesada
ME02288B (me) 2010-02-08 2016-02-20 Regeneron Pharma Miš sa zajedničkim lakim lancem
US9796788B2 (en) 2010-02-08 2017-10-24 Regeneron Pharmaceuticals, Inc. Mice expressing a limited immunoglobulin light chain repertoire
US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
AU2011266843C9 (en) * 2010-06-17 2018-03-01 Kymab Limited Animal models and therapeutic molecules
US10793829B2 (en) 2010-07-26 2020-10-06 Trianni, Inc. Transgenic mammals and methods of use thereof
CN103025884B (zh) 2010-07-26 2015-11-25 特里安尼公司 转基因动物和使用方法
US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
AU2011285919C1 (en) 2010-08-02 2015-09-17 Regeneron Pharmaceuticals, Inc. Mice that make binding proteins comprising VL domains
EP2683735A1 (fr) * 2011-03-10 2014-01-15 HCO Antibody, Inc. Molécules de type anticorps tricaténaires bispécifiques
CN103917650B (zh) 2011-08-05 2017-10-24 瑞泽恩制药公司 人源化的通用轻链小鼠
EP3839049A3 (fr) 2011-09-19 2021-10-20 Kymab Limited Anticorps, domaines variables&chaînes adaptées pour une utilisation humaine
JP2014531452A (ja) 2011-09-19 2014-11-27 カイマブ・リミテッド 動物、レパートリーおよび方法
WO2013045916A1 (fr) 2011-09-26 2013-04-04 Kymab Limited Chaînes légères substituts (cls) chimères comprenant vpreb humain
RS62942B1 (sr) 2011-10-17 2022-03-31 Regeneron Pharma Miševi sa ograničenim teškim lancem imunoglobulina
US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
SG11201405059XA (en) 2012-03-28 2014-09-26 Kymab Ltd Transgenic non-human vertebrate for the expression of class - switched, fully human, antibodies
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
US10251377B2 (en) 2012-03-28 2019-04-09 Kymab Limited Transgenic non-human vertebrate for the expression of class-switched, fully human, antibodies
PT2858487T (pt) 2012-06-12 2020-01-15 Regeneron Pharma Animais não humanos humanizados com locus de cadeias pesadas imunoglobulina restrito
LT2840892T (lt) 2013-02-20 2018-07-25 Regeneron Pharmaceuticals, Inc. Nežmogaus tipo gyvūnai su modifikuotomis imunoglobulino sunkiųjų grandinių sekomis
US9980470B2 (en) 2013-03-14 2018-05-29 Erasmus University Medical Center Antibody production
WO2014141192A1 (fr) 2013-03-15 2014-09-18 Erasmus University Medical Center Génération d'anticorps à chaînes lourdes uniquement
US9788534B2 (en) 2013-03-18 2017-10-17 Kymab Limited Animal models and therapeutic molecules
US9783618B2 (en) 2013-05-01 2017-10-10 Kymab Limited Manipulation of immunoglobulin gene diversity and multi-antibody therapeutics
US11707056B2 (en) 2013-05-02 2023-07-25 Kymab Limited Animals, repertoires and methods
US9783593B2 (en) 2013-05-02 2017-10-10 Kymab Limited Antibodies, variable domains and chains tailored for human use
CA2925723A1 (fr) 2013-10-01 2015-04-09 Kymab Limited Modeles d'animaux et molecules therapeutiques
KR20160131118A (ko) 2014-03-21 2016-11-15 리제너론 파마슈티칼스 인코포레이티드 상이한 결합 특징을 전시하는 vl 항원 결합 단백질
WO2015143414A2 (fr) * 2014-03-21 2015-09-24 Regeneron Pharmaceuticals, Inc. Animaux non humains qui produisent des protéines de liaison monodomaine
RU2761118C2 (ru) 2014-04-03 2021-12-06 АйДжиЭм БАЙОСАЙЕНСИЗ, ИНК. Модифицированная j-цепь
EP3271403A1 (fr) 2015-03-19 2018-01-24 Regeneron Pharmaceuticals, Inc. Animaux non humains qui sélectionnent des régions variables de chaînes légères qui se lient à l'antigène
EP3340786A4 (fr) * 2015-08-24 2019-03-13 Trianni, Inc. Production améliorée d'immunoglobulines
AU2016332900C1 (en) 2015-09-29 2024-07-04 Amgen Inc. ASGR inhibitors
WO2017059387A1 (fr) 2015-09-30 2017-04-06 Igm Biosciences, Inc. Molécules de liaison à chaîne j modifiée
US10813346B2 (en) 2015-12-03 2020-10-27 Trianni, Inc. Enhanced immunoglobulin diversity
JP6929877B2 (ja) 2016-02-04 2021-09-01 トリアニ・インコーポレイテッドＴｒｉａｎｎｉ， Ｉｎｃ． 免疫グロブリン産生の増強
CN112004411B (zh) * 2018-03-21 2023-05-26 晶体生物科学股份有限公司 产生人抗体的转基因鸡
CA3142165A1 (fr) 2019-06-07 2020-12-10 Amgen Inc. Constructions de liaison bispecifiques a lieurs clivables de maniere selective
EP4161969A1 (fr) 2020-06-04 2023-04-12 Amgen Inc. Constructions de liaisons bispécifiques
WO2022120033A1 (fr) 2020-12-03 2022-06-09 Amgen Inc. Constructions d'immunoglobulines à domaines de liaison multiples
EP4258865B1 (fr) * 2020-12-09 2025-09-03 Trianni, Inc. Anticorps contenant seulement une chaîne lourde

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006008548A2 (fr) * 2004-07-22 2006-01-26 Erasmus University Medical Centre Rotterdam Molecules de liaison
WO2007096779A2 (fr) * 2006-01-25 2007-08-30 Erasmus University Medical Center Rotterdam Exclusion allelique
WO2008035216A2 (fr) * 2006-09-18 2008-03-27 Erasmus University Medical Center Rotterdam Molécules de liaison
WO2008122886A2 (fr) * 2007-04-04 2008-10-16 Erasmus University Medical Center Rotterdam Manipulation 1 de la lignée germinale

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006008548A2 (fr) * 2004-07-22 2006-01-26 Erasmus University Medical Centre Rotterdam Molecules de liaison
EP1864998A2 (fr) * 2004-07-22 2007-12-12 Erasmus University Medical Center Rotterdam Molécules de liaison
WO2007096779A2 (fr) * 2006-01-25 2007-08-30 Erasmus University Medical Center Rotterdam Exclusion allelique
WO2008035216A2 (fr) * 2006-09-18 2008-03-27 Erasmus University Medical Center Rotterdam Molécules de liaison
WO2008122886A2 (fr) * 2007-04-04 2008-10-16 Erasmus University Medical Center Rotterdam Manipulation 1 de la lignée germinale

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JANSSENS RICK ET AL: "Generation of heavy-chain-only antibodies in mice", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC.; US, vol. 103, no. 41, 10 October 2006 (2006-10-10), pages 15130 - 15135, XP002494537, ISSN: 0027-8424 *
ZOU Y-R ET AL: "Cre-loxP-mediated gene replacement: a mouse strain producing humanized antibodies", CURRENT BIOLOGY, CURRENT SCIENCE, GB, vol. 4, no. 12, 1 December 1994 (1994-12-01), pages 1099 - 1103, XP024248918, ISSN: 0960-9822, [retrieved on 19941201] *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9434782B2 (en) 2009-07-08 2016-09-06 Kymab Limited Animal models and therapeutic molecules
US12486336B2 (en) 2015-09-30 2025-12-02 Igm Biosciences, Inc. Binding molecules with modified J-chain

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