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WO2009009139A3 - Rna ligase polypeptides and methods of selection and use thereof - Google Patents

Rna ligase polypeptides and methods of selection and use thereof Download PDF

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Publication number
WO2009009139A3
WO2009009139A3 PCT/US2008/008550 US2008008550W WO2009009139A3 WO 2009009139 A3 WO2009009139 A3 WO 2009009139A3 US 2008008550 W US2008008550 W US 2008008550W WO 2009009139 A3 WO2009009139 A3 WO 2009009139A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
selection
rna ligase
polypeptides
ligase polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/008550
Other languages
French (fr)
Other versions
WO2009009139A2 (en
Inventor
Burchard Seelig
Jack W Szostak
Anthony D Keefe
Glen S Cho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Original Assignee
General Hospital Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Hospital Corp filed Critical General Hospital Corp
Publication of WO2009009139A2 publication Critical patent/WO2009009139A2/en
Publication of WO2009009139A3 publication Critical patent/WO2009009139A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention features polypeptides capable of catalyzing a ligation reaction between a first RNA molecule comprising, e.g., a hydroxyl group at the 3' position and a second RNA molecule comprising, e.g., a triphosphate group at the 5' position. The invention further features methods of selecting, optimizing, and using such polypeptides.
PCT/US2008/008550 2007-07-11 2008-07-11 Rna ligase polypeptides and methods of selection and use thereof Ceased WO2009009139A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95908407P 2007-07-11 2007-07-11
US60/959,084 2007-07-11

Publications (2)

Publication Number Publication Date
WO2009009139A2 WO2009009139A2 (en) 2009-01-15
WO2009009139A3 true WO2009009139A3 (en) 2009-05-28

Family

ID=40229362

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/008550 Ceased WO2009009139A2 (en) 2007-07-11 2008-07-11 Rna ligase polypeptides and methods of selection and use thereof

Country Status (1)

Country Link
WO (1) WO2009009139A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140329700A1 (en) * 2011-08-23 2014-11-06 Rutgers, The State University Of New Jersey Methods of isolating rna and mapping of polyadenylation isoforms

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763594A (en) * 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6696473B2 (en) * 2001-12-21 2004-02-24 X-Ceptor Therapeutics, Inc. Heterocyclic modulators of nuclear receptors
US20050113292A1 (en) * 2003-07-18 2005-05-26 Vanderbilt University Compositions of protein mimetics and methods of using same against HIV-1, SARS-coV and the like
US20050266439A1 (en) * 2002-09-20 2005-12-01 Sigridur Hjorleifsdottir Thermostable RNA ligase from thermus phage
US20060281113A1 (en) * 2005-05-18 2006-12-14 George Church Accessible polynucleotide libraries and methods of use thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763594A (en) * 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6696473B2 (en) * 2001-12-21 2004-02-24 X-Ceptor Therapeutics, Inc. Heterocyclic modulators of nuclear receptors
US20050266439A1 (en) * 2002-09-20 2005-12-01 Sigridur Hjorleifsdottir Thermostable RNA ligase from thermus phage
US20050113292A1 (en) * 2003-07-18 2005-05-26 Vanderbilt University Compositions of protein mimetics and methods of using same against HIV-1, SARS-coV and the like
US20060281113A1 (en) * 2005-05-18 2006-12-14 George Church Accessible polynucleotide libraries and methods of use thereof

Also Published As

Publication number Publication date
WO2009009139A2 (en) 2009-01-15

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