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WO2009008664A3 - A producing method for gentamicin a2 or the precursors and recombinant microorganisms producing the same - Google Patents

A producing method for gentamicin a2 or the precursors and recombinant microorganisms producing the same Download PDF

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Publication number
WO2009008664A3
WO2009008664A3 PCT/KR2008/004033 KR2008004033W WO2009008664A3 WO 2009008664 A3 WO2009008664 A3 WO 2009008664A3 KR 2008004033 W KR2008004033 W KR 2008004033W WO 2009008664 A3 WO2009008664 A3 WO 2009008664A3
Authority
WO
WIPO (PCT)
Prior art keywords
producing
gentamicin
precursors
same
recombinant microorganisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2008/004033
Other languages
French (fr)
Other versions
WO2009008664A4 (en
WO2009008664A2 (en
Inventor
Jea Jeong Kim
Si Kyu Lim
Yeo Joon Yoon
Je Won Park
Mi Ok Lee
Bo-Mi Lee
Dong Hwan Kim
Jeoung Hyun Ryu
Keum Soon Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genotech Co Ltd
Original Assignee
Genotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genotech Co Ltd filed Critical Genotech Co Ltd
Publication of WO2009008664A2 publication Critical patent/WO2009008664A2/en
Publication of WO2009008664A3 publication Critical patent/WO2009008664A3/en
Publication of WO2009008664A4 publication Critical patent/WO2009008664A4/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
    • C12P19/48—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin the cyclohexyl radical being substituted by two or more nitrogen atoms, e.g. destomycin, neamin

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Disclosed herein is a method of producing aminoglycoside antibiotics containing 2-deoxystrept amine, paromamine, 2'-N-acetyl-paromamine, or gentamicin A2 by the recombination of biosynthetic genes in a heterologous host.
PCT/KR2008/004033 2007-07-09 2008-07-09 A producing method for gentamicin a2 or the precursors and recombinant microorganisms producing the same Ceased WO2009008664A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2007-0068464 2007-07-09
KR1020070068464A KR20090005436A (en) 2007-07-09 2007-07-09 Method for producing gentamicin A2 and its precursors and recombinant strains

Publications (3)

Publication Number Publication Date
WO2009008664A2 WO2009008664A2 (en) 2009-01-15
WO2009008664A3 true WO2009008664A3 (en) 2009-03-19
WO2009008664A4 WO2009008664A4 (en) 2009-05-07

Family

ID=40229272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/004033 Ceased WO2009008664A2 (en) 2007-07-09 2008-07-09 A producing method for gentamicin a2 or the precursors and recombinant microorganisms producing the same

Country Status (2)

Country Link
KR (1) KR20090005436A (en)
WO (1) WO2009008664A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110358718B (en) * 2019-07-19 2021-04-20 福州市鼓楼区荣德生物科技有限公司 Construction and application of engineering bacteria mainly producing gentamicin C1a
CN113403237B (en) * 2021-07-27 2021-12-28 青岛安惠仕生物制药有限公司 Gentamicin sulfate prepared by enhanced microbial fermentation and application method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040026067A (en) * 2002-09-17 2004-03-27 주식회사 대웅 Nucleotide sequences and amino acid sequences of deoxy-scyllo-inosose synthase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040026067A (en) * 2002-09-17 2004-03-27 주식회사 대웅 Nucleotide sequences and amino acid sequences of deoxy-scyllo-inosose synthase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JAMIE UNWIN ET AL.: "Gene cluster in micromonospora echinospora ATCC15835 of the biosynthesis of the gentamicin C complex", THE JOURNAL OF ANTIBIOTICS, vol. 57, no. 7, August 2004 (2004-08-01), pages 436 - 445 *
NICHOLAS ET AL.: "Biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics", NATURAL PRODUCT REPORTS, vol. 23, September 2006 (2006-09-01), pages 864 - 874 *
PNAISER ET AL.: "EM17 the distribution of the gantamicin gene cluster in nature", SOCIETY FOR GENERAL MICROBIOLOGY-153RD MEETING-UMIST, 2003, pages 31 *

Also Published As

Publication number Publication date
WO2009008664A4 (en) 2009-05-07
KR20090005436A (en) 2009-01-14
WO2009008664A2 (en) 2009-01-15

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