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WO2009006584A3 - Methods and compositions for identifying cellular rna transcripts - Google Patents

Methods and compositions for identifying cellular rna transcripts Download PDF

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Publication number
WO2009006584A3
WO2009006584A3 PCT/US2008/069178 US2008069178W WO2009006584A3 WO 2009006584 A3 WO2009006584 A3 WO 2009006584A3 US 2008069178 W US2008069178 W US 2008069178W WO 2009006584 A3 WO2009006584 A3 WO 2009006584A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
rna transcripts
compositions
cellular rna
identifying cellular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/069178
Other languages
French (fr)
Other versions
WO2009006584A2 (en
Inventor
Dyk Linda F Van
Kevin W Diebel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Colorado Boulder
Original Assignee
University of Colorado Boulder
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Colorado Boulder filed Critical University of Colorado Boulder
Publication of WO2009006584A2 publication Critical patent/WO2009006584A2/en
Publication of WO2009006584A3 publication Critical patent/WO2009006584A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided herein are methods to determine modifications of cellular RNA transcripts and/or map the 5' and 3' ends of RNA transcripts.
PCT/US2008/069178 2007-07-03 2008-07-03 Methods and compositions for identifying cellular rna transcripts Ceased WO2009006584A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94792807P 2007-07-03 2007-07-03
US60/947,928 2007-07-03

Publications (2)

Publication Number Publication Date
WO2009006584A2 WO2009006584A2 (en) 2009-01-08
WO2009006584A3 true WO2009006584A3 (en) 2009-03-05

Family

ID=40226821

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/069178 Ceased WO2009006584A2 (en) 2007-07-03 2008-07-03 Methods and compositions for identifying cellular rna transcripts

Country Status (1)

Country Link
WO (1) WO2009006584A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2666870A1 (en) * 2012-05-23 2013-11-27 Pathoquest Method for differential treatment of nucleic acid contents of a sample, sample enrichment, kit and uses thereof
WO2015126927A2 (en) * 2014-02-18 2015-08-27 Duke University Compositions for the inactivation of virus replication and methods of making and using the same

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FU H. ET AL.: "Identification of human fetal liver miRNAs by a novel method", FEBS LETTERS, vol. 579, no. 17, July 2005 (2005-07-01), pages 3849 - 3854, XP004961982, DOI: doi:10.1016/j.febslet.2005.05.064 *
HONDA A. ET AL.: "Identification of the 5' terminal structure of influenza virus genome RNA by a newly developed enzymatic method", VIRUS RESEARCH, vol. 55, no. 2, June 1998 (1998-06-01), pages 199 - 206 *
SUNKAR R. ET AL.: "Identification and characterization of endogenous small interfering RNAs from rice", NUCLEIC ACIDS RESEARCH, vol. 33, no. 14, 2 August 2005 (2005-08-02), pages 4443 - 4454 *
ZHANG M. ET AL.: "Identification of the 5' terminal sequence of SAR-55 amd MEX-14 strains of Hepatitis E virus and confirmation that the genome is capped", JOURNAL OF MEDICAL VIROLOGY, vol. 65, no. 2, October 2001 (2001-10-01), pages 293 - 295 *

Also Published As

Publication number Publication date
WO2009006584A2 (en) 2009-01-08

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