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WO2009005117A1 - 生体小分子の検出方法及び装置 - Google Patents

生体小分子の検出方法及び装置 Download PDF

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Publication number
WO2009005117A1
WO2009005117A1 PCT/JP2008/062055 JP2008062055W WO2009005117A1 WO 2009005117 A1 WO2009005117 A1 WO 2009005117A1 JP 2008062055 W JP2008062055 W JP 2008062055W WO 2009005117 A1 WO2009005117 A1 WO 2009005117A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
biomicromolecule
excitation light
irradiated
irradiation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/062055
Other languages
English (en)
French (fr)
Inventor
Kazunori Ikebukuro
Ryo Katayama
Eiji Takahashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kobe Steel Ltd
Tokyo University of Agriculture and Technology NUC
University of Tokyo NUC
Original Assignee
Kobe Steel Ltd
Tokyo University of Agriculture and Technology NUC
University of Tokyo NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kobe Steel Ltd, Tokyo University of Agriculture and Technology NUC, University of Tokyo NUC filed Critical Kobe Steel Ltd
Priority to US12/667,585 priority Critical patent/US8310676B2/en
Priority to EP08777807A priority patent/EP2180313A4/en
Publication of WO2009005117A1 publication Critical patent/WO2009005117A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/171—Systems in which incident light is modified in accordance with the properties of the material investigated with calorimetric detection, e.g. with thermal lens detection
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analyzing Materials Using Thermal Means (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　この発明は、簡易かつ安価な設備で、試料中の生体小分子を検出することを目的とする。そのために、試料Ｓ中の生体小分子が光学的に検出される。具体的には、生体小分子との相互作用が可能なアプタマーを含む試料Ｓに対して励起光Ｌｅが照射されるとともに、この励起光Ｌｅの照射により試料Ｓ内に生ずる光熱効果を測定するための測定光Ｌ２が照射される。この測定光Ｌ２の位相変化から、励起光Ｌｅによる試料Ｓの光熱効果が測定され、その測定信号の時間変化に基づいて生体小分子とそのアプタマーとの相互作用の発生の有無が判断される。
PCT/JP2008/062055 2007-07-04 2008-07-03 生体小分子の検出方法及び装置 Ceased WO2009005117A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/667,585 US8310676B2 (en) 2007-07-04 2008-07-03 Method and apparatus for detecting small biomolecules
EP08777807A EP2180313A4 (en) 2007-07-04 2008-07-03 METHOD AND DEVICE FOR DETECTING A BIOMICROMOLEKÜLS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007176313A JP4947520B2 (ja) 2007-07-04 2007-07-04 生体小分子の検出方法及び装置
JP2007-176313 2007-07-04

Publications (1)

Publication Number Publication Date
WO2009005117A1 true WO2009005117A1 (ja) 2009-01-08

Family

ID=40226158

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/062055 Ceased WO2009005117A1 (ja) 2007-07-04 2008-07-03 生体小分子の検出方法及び装置

Country Status (4)

Country Link
US (1) US8310676B2 (ja)
EP (1) EP2180313A4 (ja)
JP (1) JP4947520B2 (ja)
WO (1) WO2009005117A1 (ja)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5033820B2 (ja) * 2009-02-03 2012-09-26 株式会社日立ハイテクノロジーズ 全反射顕微鏡装置、及び蛍光試料分析方法
JP5967687B2 (ja) * 2009-07-17 2016-08-10 Ｎｅｃソリューションイノベータ株式会社 スフィンゴシルホスホリルコリンに結合するアプタマー分子
KR101886432B1 (ko) * 2011-12-23 2018-09-07 엘지디스플레이 주식회사 유기전계발광표시장치 및 이의 제조 방법
US10542918B2 (en) 2013-10-23 2020-01-28 Verily Life Sciences Llc Modulation of a response signal to distinguish between analyte and background signals
US9504405B2 (en) 2013-10-23 2016-11-29 Verily Life Sciences Llc Spatial modulation of magnetic particles in vasculature by external magnetic field
KR101531158B1 (ko) * 2014-03-13 2015-06-25 연세대학교 산학협력단 광열효과를 이용한 헤모글로빈 농도측정장치 및 그 방법
US9874554B1 (en) 2014-07-16 2018-01-23 Verily Life Sciences Llc Aptamer-based in vivo diagnostic system
JP2016114532A (ja) * 2014-12-17 2016-06-23 株式会社日立製作所 光熱変換分光分析装置
KR102238950B1 (ko) * 2019-02-21 2021-04-12 주식회사 아스타 극세초점 ldi 장치 및 방법

Citations (8)

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JP2001165939A (ja) * 1999-12-10 2001-06-22 Asahi Kasei Corp キャピラリー分析装置
WO2002090912A1 (en) * 2001-05-07 2002-11-14 Kanagawa Academy Of Science And Technology Methods for measuring and controlling the temperature of liquid phase in micro passage of microchip, device for the methods, and microchip
JP2004156925A (ja) * 2002-11-01 2004-06-03 Jsr Corp ラボオンチップ
JP2004301520A (ja) * 2003-03-28 2004-10-28 Kobe Steel Ltd 光熱変換測定装置及びその方法
JP2005283566A (ja) 2004-02-11 2005-10-13 Agilent Technol Inc ベースの設計方法
WO2006033348A1 (ja) * 2004-09-22 2006-03-30 National University Corporation Tokyo University Of Agriculuture And Technology 標識酵素
JP2006084431A (ja) * 2004-09-17 2006-03-30 Kobe Steel Ltd 光熱変換測定装置及びその方法
JP2006226880A (ja) 2005-02-18 2006-08-31 Hitachi High-Technologies Corp 液体クロマトグラフ質量分析方法

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JP2005283566A (ja) 2004-02-11 2005-10-13 Agilent Technol Inc ベースの設計方法
JP2006084431A (ja) * 2004-09-17 2006-03-30 Kobe Steel Ltd 光熱変換測定装置及びその方法
WO2006033348A1 (ja) * 2004-09-22 2006-03-30 National University Corporation Tokyo University Of Agriculuture And Technology 標識酵素
JP2006226880A (ja) 2005-02-18 2006-08-31 Hitachi High-Technologies Corp 液体クロマトグラフ質量分析方法

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Also Published As

Publication number Publication date
JP2009014496A (ja) 2009-01-22
US20110007317A1 (en) 2011-01-13
EP2180313A1 (en) 2010-04-28
JP4947520B2 (ja) 2012-06-06
US8310676B2 (en) 2012-11-13
EP2180313A4 (en) 2011-10-05

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