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WO2009002480A3 - Acyl transferase having altered substrate specificity - Google Patents

Acyl transferase having altered substrate specificity Download PDF

Info

Publication number
WO2009002480A3
WO2009002480A3 PCT/US2008/007807 US2008007807W WO2009002480A3 WO 2009002480 A3 WO2009002480 A3 WO 2009002480A3 US 2008007807 W US2008007807 W US 2008007807W WO 2009002480 A3 WO2009002480 A3 WO 2009002480A3
Authority
WO
WIPO (PCT)
Prior art keywords
substrate specificity
acyl transferase
altered substrate
perhydrolysis
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/007807
Other languages
French (fr)
Other versions
WO2009002480A2 (en
WO2009002480A9 (en
Inventor
Richard R Bott
Marguerite A Cervin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of WO2009002480A2 publication Critical patent/WO2009002480A2/en
Publication of WO2009002480A3 publication Critical patent/WO2009002480A3/en
Publication of WO2009002480A9 publication Critical patent/WO2009002480A9/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides methods for engineering the M. smegmatis acyltransferase to create compositions comprising at least one enzyme suitable for use in enzymatic aqueous acylation and/or perhydrolysis. The present invention further provides compositions comprising at least one perhydrolase enzyme suitable for use in enzymatic aqueous acylation and/or perhydrolysis.
PCT/US2008/007807 2007-06-26 2008-06-23 Acyl transferase having altered substrate specificity Ceased WO2009002480A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93741707P 2007-06-26 2007-06-26
US60/937,417 2007-06-26

Publications (3)

Publication Number Publication Date
WO2009002480A2 WO2009002480A2 (en) 2008-12-31
WO2009002480A3 true WO2009002480A3 (en) 2009-02-19
WO2009002480A9 WO2009002480A9 (en) 2009-04-09

Family

ID=39950146

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/007807 Ceased WO2009002480A2 (en) 2007-06-26 2008-06-23 Acyl transferase having altered substrate specificity

Country Status (1)

Country Link
WO (1) WO2009002480A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1387616T3 (en) 2001-05-18 2007-09-24 Danisco Process for preparing a dough with an enzyme
ES2539006T3 (en) 2004-07-16 2015-06-25 Dupont Nutrition Biosciences Aps Lipolytic enzyme, uses thereof in the food industry
GB0807161D0 (en) * 2008-04-18 2008-05-21 Danisco Process
MX389888B (en) * 2008-12-23 2025-03-20 Genomatica Inc METHODS AND COMPOSITIONS RELATED TO THIOESTERASE ENZYME.
WO2012010406A1 (en) 2010-07-22 2012-01-26 Unilever Plc Combinations of rhamnolipids and enzymes for improved cleaning
AR084876A1 (en) 2011-01-21 2013-07-10 Novozymes As PRODUCTION OF ALKYL ESTERS OF FATTY ACIDS
BR122019026127B1 (en) 2012-04-02 2021-12-21 Genomatica, Inc. RECOMBINANT HOST CELL AND CELL CULTURE
WO2017060471A1 (en) 2015-10-09 2017-04-13 Novozymes A/S Enzymatic or non-enzymatic biodiesel polishing process
EP3472335B1 (en) 2016-06-16 2024-12-25 Novozymes A/S Reduction of phospholipids in phospholipid-containing oil material
US20240218404A1 (en) 2021-05-04 2024-07-04 Novozymes A/S Enzymatic treatment of feedstock for hvo production
CN115806952B (en) * 2022-09-14 2024-05-03 福建师范大学 Mycobacterium smegmatis acyltransferase mutant capable of being efficiently coupled with glucose oxidase and preparation method and application thereof
CN116855470B (en) * 2023-07-10 2024-06-11 态创生物科技(广州)有限公司 Acyltransferase MsAcT variant, fusion protein, immobilization method and application thereof
CN117625506A (en) * 2023-11-29 2024-03-01 可莱尼化妆品科技有限公司 An engineering bacterium for producing N-acetyl-trans-4-hydroxyproline and its application
CN119776314B (en) * 2024-12-31 2025-11-11 可莱尼化妆品科技有限公司 Acyltransferase mutant and application thereof in synthesis of N-acetyl-trans-4-hydroxyproline

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005056782A2 (en) * 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
WO2007133263A2 (en) * 2005-12-09 2007-11-22 Genencor International, Inc. Acyl transferase useful for decontamination
WO2008019069A2 (en) * 2006-08-03 2008-02-14 Danisco Us, Inc., Genencor Division Enzymatic aqueous acylation
WO2008063400A1 (en) * 2006-11-09 2008-05-29 Danisco Us, Inc., Genencor Division Enzyme for the production of long chain peracid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005056782A2 (en) * 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
WO2007133263A2 (en) * 2005-12-09 2007-11-22 Genencor International, Inc. Acyl transferase useful for decontamination
WO2008019069A2 (en) * 2006-08-03 2008-02-14 Danisco Us, Inc., Genencor Division Enzymatic aqueous acylation
WO2008063400A1 (en) * 2006-11-09 2008-05-29 Danisco Us, Inc., Genencor Division Enzyme for the production of long chain peracid

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BERNHARDT P ET AL: "MOLECULAR BASIS OF PERHYDROLASE ACTIVITY IN SERINE HYDROLASES", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, VCH VERLAG, WEINHEIM, DE, vol. 44, no. 18, 29 April 2005 (2005-04-29), pages 2742 - 2746, XP008047813, ISSN: 0570-0833 *
DATABASE Geneseq [online] 24 July 2008 (2008-07-24), "Mycobacterium smegmatis arylesterase SEQ ID 1.", retrieved from EBI accession no. GSP:AQX98615 Database accession no. AQX98615 *
DATABASE Geneseq [online] 24 July 2008 (2008-07-24), "Mycobacterium smegmatis mutant perhydrolase (L12G,F154G).", retrieved from EBI accession no. GSP:ARW66032 Database accession no. ARW66032 *
DATABASE Geneseq [online] 25 August 2005 (2005-08-25), "M. gilvum perhydrolase homolog 14B.", XP002505225, retrieved from EBI accession no. GSP:AEA43714 Database accession no. AEA43714 *
DATABASE Geneseq [online] 25 August 2005 (2005-08-25), "M. parafortuitum perhydrolase homolog 20A.", XP002505226, retrieved from EBI accession no. GSP:AEA43716 Database accession no. AEA43716 *
DATABASE Geneseq [online] 25 August 2005 (2005-08-25), "Perhydrolase homolog SEQ ID NO 695.", retrieved from EBI accession no. GSP:AEA43769 Database accession no. AEA43769 *
MATHEWS IRIMPAN ET AL: "Structure of a novel enzyme that catalyzes acyl transfer to alcohols in aqueous conditions", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, EASTON, PA.; US, vol. 46, no. 31, 1 August 2007 (2007-08-01), pages 8969 - 8979, XP002465298, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
WO2009002480A2 (en) 2008-12-31
WO2009002480A9 (en) 2009-04-09

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