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WO2009097626A3 - Eléments de lecture à extrémités appariées dans le séquençage par synthèse - Google Patents

Eléments de lecture à extrémités appariées dans le séquençage par synthèse Download PDF

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Publication number
WO2009097626A3
WO2009097626A3 PCT/US2009/032970 US2009032970W WO2009097626A3 WO 2009097626 A3 WO2009097626 A3 WO 2009097626A3 US 2009032970 W US2009032970 W US 2009032970W WO 2009097626 A3 WO2009097626 A3 WO 2009097626A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing
synthesis
methods
paired
end reads
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/032970
Other languages
English (en)
Other versions
WO2009097626A2 (fr
Inventor
Timothy Harris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helicos BioSciences Corp
Original Assignee
Helicos BioSciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/025,033 external-priority patent/US7767400B2/en
Application filed by Helicos BioSciences Corp filed Critical Helicos BioSciences Corp
Priority to EP09706204A priority Critical patent/EP2247741A4/fr
Publication of WO2009097626A2 publication Critical patent/WO2009097626A2/fr
Publication of WO2009097626A3 publication Critical patent/WO2009097626A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés de génération d'éléments de lecture appariés dans un procédé de séquençage par synthèse, en particulier dans des systèmes présentant des longueurs d'éléments de lecture relativement courtes (par exemple, 15-35 bases), tels que par exemple, dans le séquençage d'une molécule unique par synthèse. Plusieurs mises en œuvre de ces procédés sont proposées. Les procédés qui permettent le re-séquençage de la matrice, qui donne des taux d'erreurs inférieurs, sont particulièrement avantageux. L'invention concerne en outre des procédés d'utilisations d'éléments de lecture appariés, par exemple, pour les placer sur des répétitions ou pour les assembler dans de grandes séquences, comprenant l'ensemble du génome complet.
PCT/US2009/032970 2008-02-03 2009-02-03 Eléments de lecture à extrémités appariées dans le séquençage par synthèse Ceased WO2009097626A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09706204A EP2247741A4 (fr) 2008-02-03 2009-02-03 Eléments de lecture à extrémités appariées dans le séquençage par synthèse

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US12/025,033 US7767400B2 (en) 2008-02-03 2008-02-03 Paired-end reads in sequencing by synthesis
US12/025,033 2008-02-03
US4739708P 2008-04-23 2008-04-23
US61/047,397 2008-04-23
US9891008P 2008-09-22 2008-09-22
US61/098,910 2008-09-22

Publications (2)

Publication Number Publication Date
WO2009097626A2 WO2009097626A2 (fr) 2009-08-06
WO2009097626A3 true WO2009097626A3 (fr) 2009-09-24

Family

ID=40913521

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/032970 Ceased WO2009097626A2 (fr) 2008-02-03 2009-02-03 Eléments de lecture à extrémités appariées dans le séquençage par synthèse

Country Status (2)

Country Link
EP (1) EP2247741A4 (fr)
WO (1) WO2009097626A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002044425A2 (fr) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Synthese d'acides nucleiques d'enzymes, et compositions et methodes modifiant la fidelite d'incorporation de monomeres
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
WO2010127304A2 (fr) * 2009-05-01 2010-11-04 Illumina, Inc. Procédés de séquençage
DK2456892T3 (en) 2009-07-24 2014-12-08 Illumina Inc Procedure for sequencing of a polynukleotidskabelon
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
US8753816B2 (en) * 2010-10-26 2014-06-17 Illumina, Inc. Sequencing methods
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
CA2891939C (fr) 2012-11-19 2020-10-27 Apton Biosystems, Inc. Analyse numerique d'analytes moleculaires au moyen d'une detection de molecules individuelles
CN105112516A (zh) * 2015-08-14 2015-12-02 深圳市瀚海基因生物科技有限公司 一种单分子靶向测序方法、装置、系统及应用
CN114921537A (zh) * 2017-03-17 2022-08-19 雅普顿生物系统公司 测序和高分辨率成像
WO2020018824A1 (fr) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Procédés, systèmes et kits d'amplification et de séquençage clonale d'acide nucléique
WO2020061237A1 (fr) * 2018-09-19 2020-03-26 Apton Biosystems, Inc. Couches d'analyte tassées de manière dense et procédés de détection
AU2020412459B2 (en) 2019-12-23 2022-12-08 Singular Genomics Systems, Inc. Methods for long read sequencing

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030044796A1 (en) * 1996-11-29 2003-03-06 Neri Bruce P. Reactions on dendrimers
US20030073081A1 (en) * 1999-03-19 2003-04-17 Takara Shuzo Co., Ltd Method for amplifying nucleic acid sequence
US20030143535A1 (en) * 2000-06-17 2003-07-31 Third Wave Technologies, Inc. Nucleic acid accessible hybridization sites
US20030182068A1 (en) * 2001-10-30 2003-09-25 Battersby Bronwyn J. Device and methods for directed synthesis of chemical libraries
US20060040297A1 (en) * 2003-01-29 2006-02-23 Leamon John H Methods of amplifying and sequencing nucleic acids
US20070099212A1 (en) * 2005-07-28 2007-05-03 Timothy Harris Consecutive base single molecule sequencing

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030044796A1 (en) * 1996-11-29 2003-03-06 Neri Bruce P. Reactions on dendrimers
US20030073081A1 (en) * 1999-03-19 2003-04-17 Takara Shuzo Co., Ltd Method for amplifying nucleic acid sequence
US20030143535A1 (en) * 2000-06-17 2003-07-31 Third Wave Technologies, Inc. Nucleic acid accessible hybridization sites
US20030182068A1 (en) * 2001-10-30 2003-09-25 Battersby Bronwyn J. Device and methods for directed synthesis of chemical libraries
US20060040297A1 (en) * 2003-01-29 2006-02-23 Leamon John H Methods of amplifying and sequencing nucleic acids
US20070099212A1 (en) * 2005-07-28 2007-05-03 Timothy Harris Consecutive base single molecule sequencing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2247741A4 *

Also Published As

Publication number Publication date
EP2247741A2 (fr) 2010-11-10
WO2009097626A2 (fr) 2009-08-06
EP2247741A4 (fr) 2011-02-23

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