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WO2009096429A1 - Procédé pour l'extraction d'un nucléotide - Google Patents

Procédé pour l'extraction d'un nucléotide Download PDF

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Publication number
WO2009096429A1
WO2009096429A1 PCT/JP2009/051364 JP2009051364W WO2009096429A1 WO 2009096429 A1 WO2009096429 A1 WO 2009096429A1 JP 2009051364 W JP2009051364 W JP 2009051364W WO 2009096429 A1 WO2009096429 A1 WO 2009096429A1
Authority
WO
WIPO (PCT)
Prior art keywords
atp
nucleotide
extraction
reagent
measurement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2009/051364
Other languages
English (en)
Japanese (ja)
Inventor
Hiroshi Kido
Junji Chida
Tsunetomo Takei
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Tokushima NUC
Original Assignee
University of Tokushima NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Tokushima NUC filed Critical University of Tokushima NUC
Priority to JP2009551540A priority Critical patent/JP5522665B2/ja
Publication of WO2009096429A1 publication Critical patent/WO2009096429A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders

Definitions

  • Congenital diseases include mitochondrial encephalomyopathy (CPEO: chronic progressive external ophthalmoplegia, MELAS: mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes, MERRF: myoclonus epilepsy associated with ragged red fibers)
  • CPEO chronic progressive external ophthalmoplegia
  • MELAS mitochondrial myopathy
  • encephalopathy lactic acidosis and stroke-like episodes
  • MERRF myoclonus epilepsy associated with ragged red fibers
  • cerebral infarction As acquired diseases, cerebral infarction, neurodegenerative diseases of the brain (Alzheimer's mania, Parkinson's disease, Huntington's disease, viral encephalopathy) and the like have been reported.
  • these diseases are characterized by general clinical symptoms such as convulsions, epilepsy, psychomotor development delay, muscle weakness, liver dysfunction, and sensorineural hearing loss.
  • ATP level in peripheral blood was low (1 mM or less) in patients with encephalopathy (acute stage) and mitochondrial encephalomyopathy, but in patients with encephalopathy recovery stage, The ATP level in the blood was 1.2 ⁇ m or more, and it was confirmed that the ATP level was recovered (see FIG. 13).
  • the extraction solution used in the nucleotide extraction method of the present invention can be used as a nucleotide extraction reagent, and can be included in a reagent kit for measuring ATP, GTP, or UTP.
  • a reagent kit for measuring ATP, GTP, or UTP can be included in a reagent kit for measuring ATP, GTP, or UTP.
  • the extraction method of the present invention can also be used as an ATP extraction reagent attached to an existing ATP measurement reagent.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé d'extraction d'un nucléotide dans un échantillon d'une manière simple et très efficace. L'invention concerne également un réactif d'extraction de nucléotides. L'invention concerne en outre une trousse de réactifs pour mesurer un nucléotide, laquelle comprend le réactif d'extraction de nucléotides. Un nucléotide (par ex., ATP, GTP et/ou UTP) dans un échantillon peut être extrait de l'échantillon avec un degré élevé d'efficacité en utilisant une solution qui contient un composé phénolique. Un réactif d'extraction comprenant un composé phénolique peut être utilisé dans la mesure de l'ATP ou similaires. Il devient également possible d'obtenir une trousse de réactifs pour mesurer l'ATP, laquelle comprend le réactif d'extraction.
PCT/JP2009/051364 2008-01-29 2009-01-28 Procédé pour l'extraction d'un nucléotide Ceased WO2009096429A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009551540A JP5522665B2 (ja) 2008-01-29 2009-01-28 ヌクレオチドの抽出方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008017863 2008-01-29
JP2008-017863 2008-01-29

Publications (1)

Publication Number Publication Date
WO2009096429A1 true WO2009096429A1 (fr) 2009-08-06

Family

ID=40912781

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2009/051364 Ceased WO2009096429A1 (fr) 2008-01-29 2009-01-28 Procédé pour l'extraction d'un nucléotide

Country Status (2)

Country Link
JP (1) JP5522665B2 (fr)
WO (1) WO2009096429A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011152012A1 (fr) 2010-05-31 2011-12-08 国立大学法人徳島大学 Procédé pour analyser la gravité d'une maladie
CN102596010A (zh) * 2009-08-10 2012-07-18 P2科学有限公司 用于诊断狭窄和其他血流受限之病症的utp

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ARRETXE, M. ET AL.: "The effect of toxic discharges on ATP content in activated sludge", ENVIRONMENTAL TOXICOLOGY AND WATER QUALITY, vol. 12, 1997, pages 23 - 29 *
NOACK, H. ET AL.: "Evaluation of a Procedure for the Simultaneous Determination of Oxidized and Reduced Pyridine Nucleotides and Adenylates in Organic Phenol Extracts from Mitochondria", ANALYTICAL BIOCHEMISTRY, vol. 202, 1992, pages 162 - 165 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102596010A (zh) * 2009-08-10 2012-07-18 P2科学有限公司 用于诊断狭窄和其他血流受限之病症的utp
WO2011152012A1 (fr) 2010-05-31 2011-12-08 国立大学法人徳島大学 Procédé pour analyser la gravité d'une maladie
US20130071866A1 (en) * 2010-05-31 2013-03-21 The University Of Tokushima Method for testing the severtiy of an illness
CN103097891A (zh) * 2010-05-31 2013-05-08 国立大学法人德岛大学 疾病重症度的检查方法
EP2579037A4 (fr) * 2010-05-31 2013-10-30 Univ Tokushima Procédé pour analyser la gravité d'une maladie
JP5916130B2 (ja) * 2010-05-31 2016-05-11 国立大学法人徳島大学 病気の重症度の検査方法

Also Published As

Publication number Publication date
JPWO2009096429A1 (ja) 2011-05-26
JP5522665B2 (ja) 2014-06-18

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