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WO2009075119A1 - 効率的な核初期化方法 - Google Patents

効率的な核初期化方法 Download PDF

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Publication number
WO2009075119A1
WO2009075119A1 PCT/JP2008/059586 JP2008059586W WO2009075119A1 WO 2009075119 A1 WO2009075119 A1 WO 2009075119A1 JP 2008059586 W JP2008059586 W JP 2008059586W WO 2009075119 A1 WO2009075119 A1 WO 2009075119A1
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Prior art keywords
mirna
nucleus initialization
initialization method
stem cells
effective nucleus
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Ceased
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PCT/JP2008/059586
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English (en)
French (fr)
Inventor
Shinya Yamanaka
Michiyo Koyanagi
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Kyoto University NUC
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Kyoto University NUC
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Priority to AU2008286249A priority Critical patent/AU2008286249B2/en
Priority to KR1020097005179A priority patent/KR101532442B1/ko
Priority to CA2658463A priority patent/CA2658463C/en
Priority to JP2009510625A priority patent/JP5558097B2/ja
Priority to US12/292,717 priority patent/US20100075421A1/en
Priority to US12/379,564 priority patent/US9683232B2/en
Publication of WO2009075119A1 publication Critical patent/WO2009075119A1/ja
Anticipated expiration legal-status Critical
Priority to US13/313,670 priority patent/US8791248B2/en
Ceased legal-status Critical Current

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Abstract

　人工多能性幹細胞の効率的な製造方法であって、miRNAの存在下で核初期化因子により核初期化する工程を含み、該miRNAが(a)胚性幹細胞において体細胞よりも高度に発現しており、かつ(b)該miRNAの存在下において該miRNAの非存在下の場合よりも高い核初期化効率を与える性質を有するmiRNAである上記の方法。
PCT/JP2008/059586 2007-12-10 2008-05-23 効率的な核初期化方法 Ceased WO2009075119A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2008286249A AU2008286249B2 (en) 2007-12-10 2008-05-23 Efficient method for nuclear reprogramming
KR1020097005179A KR101532442B1 (ko) 2007-12-10 2008-05-23 효율적인 핵 초기화 방법
CA2658463A CA2658463C (en) 2007-12-10 2008-05-23 Efficient method for nuclear reprogramming
JP2009510625A JP5558097B2 (ja) 2007-12-10 2008-05-23 効率的な核初期化方法
US12/292,717 US20100075421A1 (en) 2007-12-10 2008-11-25 Efficient method for nuclear reprogramming
US12/379,564 US9683232B2 (en) 2007-12-10 2009-02-25 Efficient method for nuclear reprogramming
US13/313,670 US8791248B2 (en) 2007-12-10 2011-12-07 Nuclear reprogramming factor comprising miRNA and a protein factor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US99689307P 2007-12-10 2007-12-10
US60/996,893 2007-12-10

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/292,717 Continuation-In-Part US20100075421A1 (en) 2007-12-10 2008-11-25 Efficient method for nuclear reprogramming

Publications (1)

Publication Number Publication Date
WO2009075119A1 true WO2009075119A1 (ja) 2009-06-18

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Application Number Title Priority Date Filing Date
PCT/JP2008/059586 Ceased WO2009075119A1 (ja) 2007-12-10 2008-05-23 効率的な核初期化方法

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US (2) US20100075421A1 (ja)
JP (1) JP5558097B2 (ja)
KR (1) KR101532442B1 (ja)
AU (1) AU2008286249B2 (ja)
WO (1) WO2009075119A1 (ja)

Cited By (66)

* Cited by examiner, † Cited by third party
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JP2010539934A (ja) * 2007-10-29 2010-12-24 リン、シー−ラン イントロンｒｎａを用いたヒト胚性幹様細胞の生成
JP2010539953A (ja) * 2008-01-16 2010-12-24 リン、シー−ラン 誘導性組換えｒｎａ因子を用いた腫瘍のない多能性胚性幹様細胞の生成
WO2011016588A1 (en) 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells
JP2011505814A (ja) * 2007-12-14 2011-03-03 ビオエヌテヒ・アクチエンゲゼルシャフト 体細胞を再プログラムするためのｒｎａの使用
WO2011102444A1 (ja) * 2010-02-18 2011-08-25 国立大学法人大阪大学 誘導多能性幹細胞の製造方法
WO2012074106A1 (ja) 2010-12-03 2012-06-07 国立大学法人京都大学 多能性幹細胞からの好酸球の製造方法
WO2013058403A1 (ja) 2011-10-21 2013-04-25 国立大学法人京都大学 層流による多能性維持単一分散細胞培養法
JP2013091611A (ja) * 2011-10-25 2013-05-16 Gifu Univ 間葉系細胞の分化調節剤およびこれを用いた医薬、並びに間葉系細胞への分化調節作用を有する物質のスクリーニング方法
WO2013077423A1 (ja) 2011-11-25 2013-05-30 国立大学法人京都大学 多能性幹細胞の培養方法
WO2013176233A1 (ja) 2012-05-23 2013-11-28 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
WO2014123242A1 (ja) 2013-02-08 2014-08-14 国立大学法人京都大学 巨核球及び血小板の製造方法
WO2014136581A1 (ja) 2013-03-06 2014-09-12 国立大学法人京都大学 多能性幹細胞の培養システム及び多能性幹細胞の継代方法
WO2014148646A1 (ja) 2013-03-21 2014-09-25 国立大学法人京都大学 神経分化誘導用の多能性幹細胞
WO2014157257A1 (ja) 2013-03-25 2014-10-02 公益財団法人先端医療振興財団 細胞の選別方法
WO2014168264A1 (ja) 2013-04-12 2014-10-16 国立大学法人京都大学 肺胞上皮前駆細胞の誘導方法
WO2014185358A1 (ja) 2013-05-14 2014-11-20 国立大学法人京都大学 効率的な心筋細胞の誘導方法
WO2014192909A1 (ja) 2013-05-31 2014-12-04 ｉＨｅａｒｔ Ｊａｐａｎ株式会社 ハイドロゲルを組み込んだ積層化細胞シート
WO2014200115A1 (ja) 2013-06-11 2014-12-18 国立大学法人京都大学 腎前駆細胞の製造方法及び腎前駆細胞を含む医薬
WO2015020113A1 (ja) 2013-08-07 2015-02-12 国立大学法人京都大学 膵ホルモン産生細胞の製造法
WO2015030149A1 (ja) 2013-08-29 2015-03-05 国立大学法人鳥取大学 細胞のアンチエイジングに関連する生体分子群
WO2015034012A1 (ja) 2013-09-05 2015-03-12 国立大学法人京都大学 新規ドーパミン産生神経前駆細胞の誘導方法
WO2015064754A1 (ja) 2013-11-01 2015-05-07 国立大学法人京都大学 新規軟骨細胞誘導方法
JP2015522257A (ja) * 2012-06-13 2015-08-06 ステムジェント， インコーポレイテッドＳｔｅｍｇｅｎｔ， Ｉｎｃ． 多能性幹細胞を調製する方法
JP2015534830A (ja) * 2012-11-02 2015-12-07 ロンザ ウォーカーズビル マイクロｒｎａ及び細胞リプログラミング
EP3081638A1 (en) 2015-04-16 2016-10-19 Kyoto University Method for producing pseudo-islets
US9476041B2 (en) 2010-07-12 2016-10-25 National University Corporation Tottori University Method for producing novel hipsc by means of siRNA introduction
US9512407B2 (en) 2009-09-07 2016-12-06 Genome Research Limited Cells and methods for obtaining them
US9745549B2 (en) 2012-10-19 2017-08-29 Somar Corp. Cell culture substrate, and cell culturing method using the substrate and method for inducing differentiation of pluripotent stem cells using the substrate
WO2017183736A1 (ja) 2016-04-22 2017-10-26 国立大学法人京都大学 ドーパミン産生神経前駆細胞の製造方法
EP3305899A1 (en) 2011-07-25 2018-04-11 Kyoto University Method for screening induced pluripotent stem cells
WO2018124118A1 (ja) 2016-12-27 2018-07-05 住友化学株式会社 人工多能性幹細胞の評価方法及び選抜方法、並びに人工多能性幹細胞の製造方法
WO2018135646A1 (ja) 2017-01-20 2018-07-26 国立大学法人京都大学 ＣＤ８α＋β＋細胞傷害性Ｔ細胞の製造方法
WO2018139548A1 (ja) 2017-01-26 2018-08-02 国立大学法人大阪大学 幹細胞の中胚葉系細胞への分化誘導用培地および中胚葉系細胞の製造方法
WO2018168829A1 (ja) 2017-03-14 2018-09-20 国立大学法人京都大学 多能性幹細胞からヘルパーｔ細胞を製造する方法
WO2018216743A1 (ja) 2017-05-25 2018-11-29 国立大学法人京都大学 中間中胚葉細胞から腎前駆細胞への分化誘導方法、および多能性幹細胞から腎前駆細胞への分化誘導方法
WO2018235583A1 (ja) 2017-06-19 2018-12-27 公益財団法人神戸医療産業都市推進機構 多能性幹細胞の分化能の予測方法及びそのための試薬
WO2019078263A1 (ja) 2017-10-17 2019-04-25 国立大学法人京都大学 多能性幹細胞から人工神経筋接合部を得る方法
WO2020013315A1 (ja) 2018-07-13 2020-01-16 国立大学法人京都大学 γδＴ細胞の製造方法
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US10711249B2 (en) 2014-12-26 2020-07-14 Kyoto University Method for inducing hepatocytes
WO2020235319A1 (ja) 2019-05-20 2020-11-26 味の素株式会社 軟骨又は骨の前駆細胞の拡大培養方法
WO2021117886A1 (ja) 2019-12-12 2021-06-17 国立大学法人千葉大学 巨核球および血小板を含む凍結乾燥製剤
WO2021174004A1 (en) 2020-02-28 2021-09-02 Millennium Pharmaceuticals, Inc. Method for producing natural killer cells from pluripotent stem cells
WO2021256522A1 (ja) 2020-06-17 2021-12-23 国立大学法人京都大学 キメラ抗原受容体発現免疫担当細胞
EP3929302A1 (en) 2014-07-14 2021-12-29 Chugai Seiyaku Kabushiki Kaisha Method for identifying epitope on protein
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WO2022019152A1 (ja) 2020-07-20 2022-01-27 学校法人 愛知医科大学 多能性細胞の未分化維持培養用組成物、多能性細胞の未分化維持培養用培地、多能性細胞の未分化状態での維持培養方法、および多能性細胞の製造方法
WO2022039279A1 (ja) 2020-08-18 2022-02-24 国立大学法人京都大学 ヒト始原生殖細胞／ヒト始原生殖細胞様細胞の維持増幅方法
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