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WO2009069980A3 - Protein chip for determining kinase or phosphatase activity - Google Patents

Protein chip for determining kinase or phosphatase activity Download PDF

Info

Publication number
WO2009069980A3
WO2009069980A3 PCT/KR2008/007069 KR2008007069W WO2009069980A3 WO 2009069980 A3 WO2009069980 A3 WO 2009069980A3 KR 2008007069 W KR2008007069 W KR 2008007069W WO 2009069980 A3 WO2009069980 A3 WO 2009069980A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
chip
kinase
phosphatase
protein chip
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2008/007069
Other languages
French (fr)
Other versions
WO2009069980A2 (en
Inventor
Moon-Hi Han
Hye-Jin Lim
Yong-Wan Cho
Yoon-Suk Lee
In-Cheol Kang
Eun-Kyoung Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Proteogen Inc
Original Assignee
Proteogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteogen Inc filed Critical Proteogen Inc
Publication of WO2009069980A2 publication Critical patent/WO2009069980A2/en
Publication of WO2009069980A3 publication Critical patent/WO2009069980A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed is a Well-on-a-Chip type protein chip for determining kinase or phosphatase activity, in which a Calixcrown derivative capable of recognizing a cationic functional group of a protein is immobilized on a chip base plate, and a substrate protein of kinase or phosphatase is immobilized by multiple ion recognition of the calixcrown derivative included in a well. Also, a method using the disclosed protein chip includes the steps of: a) treating the protein chip with kinase or phosphatase capable of reacting with a substrate protein; and b) measuring phosphorylation extent of the substrate protein by the treatment. Through the disclosed protein chip and method, it is possible to simply analyze the activity of kinase or phosphatase on a chip base plate by using a small amount of reagent.
PCT/KR2008/007069 2007-11-29 2008-11-28 Protein chip for determining kinase or phosphatase activity Ceased WO2009069980A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2007-0122885 2007-11-29
KR20070122885 2007-11-29

Publications (2)

Publication Number Publication Date
WO2009069980A2 WO2009069980A2 (en) 2009-06-04
WO2009069980A3 true WO2009069980A3 (en) 2009-09-03

Family

ID=40679158

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/007069 Ceased WO2009069980A2 (en) 2007-11-29 2008-11-28 Protein chip for determining kinase or phosphatase activity

Country Status (2)

Country Link
KR (1) KR20100099138A (en)
WO (1) WO2009069980A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3173788A3 (en) * 2012-03-14 2017-07-12 Marx, Stephen Means and methods for diagnostics and therapeutics of diseases
WO2013152967A1 (en) 2012-04-13 2013-10-17 Universite Libre De Bruxelles (Ulb) Materials coated with calixarenes
CN108823180A (en) * 2018-06-29 2018-11-16 云南大学 Make the dephosphorylized albumen of protein kinase B specific site and its nucleic acid
KR20210128502A (en) * 2019-03-15 2021-10-26 주식회사 이노파마스크린 Calix crown and uses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006031846A2 (en) * 2004-09-14 2006-03-23 Ut-Battelle, Llc Self-assembled monolayers on the surface of gold coated microcantilevers for application to chemical sensing and method of operation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006031846A2 (en) * 2004-09-14 2006-03-23 Ut-Battelle, Llc Self-assembled monolayers on the surface of gold coated microcantilevers for application to chemical sensing and method of operation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KIN-YA TOMIZAKI ET AL.: "Protein-detecting microarrays: Current accomplishments and requirements.", CHEM BIO CHEM., vol. 6, 2005, pages 782 - 799 *
SANG WOOK OH ET AL.: "Calixarene derivative as a tool for highly sensitive detection and oriented immobilization of proteins in a microarray format through noncovalent molecular interaction.", THE FASEB JOURNAL., vol. 19, 2005, pages 1335 - 1337 *
SHENG ZHANG ET AL.: "Selective anion sensing based on tetra-amide calkix[6]arene derivatives in solution and immobilized on gold surfaces via self-assembled mololayer.", LANGMUIR., vol. 22, 2006, pages 10732 - 10738 *

Also Published As

Publication number Publication date
KR20100099138A (en) 2010-09-10
WO2009069980A2 (en) 2009-06-04

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