WO2009066785A1 - Processed fiber product, and method for production thereof - Google Patents

Abstract

A processed fiber product having excellent strength, water-absorbability and washing durability can be produced by attaching a partially hydrolyzed product of a wheat protein to a fiber and then allowing a transglutaminase to act on the fiber.

Description

 Processed fiber and its manufacturing method

 The present invention relates to a processed fiber using transdal protein, protein and peptide, and a method for producing the same. Background art

 The textile substrate for clothing was the last polyester that appeared in the 1950s, and no remarkable new fiber substrate has been developed since then. 1 9 δ From the 0's onward, if there are insufficient properties of the fiber substrate itself, so-called fiber processing, such as spinning, is added, or a function is added later by chemical processing. ing. Improvements such as improving the wrinkle resistance of cotton, preventing shrinkage of wool, and chemically improving the shininess and sliminess of the surface of nylon and polyester are popular.

 Since the 1990s, so-called enzyme processing has been developed that uses natural fibers as an evening target and uses enzymes to compensate for the shortcomings of natural fibers as clothing materials. Cellulose hydrolyzing enzyme, which is representative of cotton, is partially hydrolyzed by cellulose hydrolyzing enzyme to obtain a softer and more textured fiber, and wool is targeted at the surface of wool cuticles. Processing that has been partially processed with enzymes to improve washing shrinkage of wool has been studied, and some have been put to practical use. In the same process, synthetic fibers are also being targeted. Synthetic polymers that were originally thought to be non-substrates have been found to have some effects on enzymes, and the surface of enzymes such as nylon and acrylic and polyester is processed with enzymes. Attempts have started.

However, the application of enzymes to fiber processing has become very active. On the other hand, most of the enzymes used in the above processing are hydrolytic enzymes, and the effects other than processing are that the surface of the fiber substrate is scraped off appropriately. Cannot be expected, There is a big limitation on the function. In order to expect the fiber substrate to have a function that it does not originally have, it is desired to use an enzyme that catalyzes a chemical bonding reaction, not a hydrolase.

 Transdalinase is one of the attractive enzymes that can satisfy the above-mentioned requirements. It binds dartamine and lysine residues in proteins, or incorporates primary amines into glutamine residues. It is an enzyme that catalyzes its action and is likely to be used in processing that acts on a polyamide-based fiber substrate and actively imparts new functions. In fact, in the field of textiles, several new processing methods have already been proposed that use transdermal taminase, mainly wool fibers.

 Since transglutaminase catalyzes the reaction of binding glutamine and lysine, it is expected to act on fiber substrates with glutamine and lysine residues, or similar residues. In fact, treatment with transglutaminase with wool as a target has been found to have effects such as cross-linking of glutamine and lysine residues in the wool substrate by enzyme-catalyzed reactions, increasing the strength of the wool. (Enzyme and M ic rob ial Techno l ogy 34 (2004) p64-72) o

 The above-mentioned action is a new function-imparting process that cannot be expected with the use of cellulose hydrolase and protein hydrolase, which have been studied for practical use so far. It can be done. However, the processing as described above requires that the fiber substrate has both glutamine and lysine residues at the same time, and the applicable fiber substrate is limited to some natural fibers such as wool.

Polyamide fibers other than wool, such as silk and naiguchi, do not have sufficient amounts of dartamine and lysine residues or similar residues that transdaltaminase acts on. Even if it is done, a crosslinking reaction cannot be expected. In order to expect a binding or cross-linking reaction to such a substrate using transdal eveningase, it is necessary to add a third component having many reactive residues that are insufficient. For example, taking a silk fiber substrate as an example, silk contains very little lysine and dartamine residues. Therefore, even if it is treated as it is with transdal evening minase, the possibility of mutual reaction is low. When processed in this way, the density of residues that can participate in the reaction increases, and the fiber substrate and the third component are bound together, resulting in an effective crosslinking reaction. In addition, if various functional materials are added in advance to the third component to be introduced, there is a possibility that a functional substance can be effectively introduced into the fiber substrate using an enzyme-catalyzed reaction.

 Even if a synthetic fiber substrate such as nylon is used, if there is a reactive residue that can be a substrate for transdal ligase, the addition of the third component causes the residue of the synthetic fiber substrate to react with the third component. As in the case of silk, a cross-linking reaction via the third component and efficient introduction of functional substances may be possible. From such a point of view, a method of coating the surface of gelatin ^ polyester (JP-A-Hei 9 37 7 2) has been proposed, and it is disclosed that fibers with high moisture permeability and moisture absorption can be obtained by coating gelatin. Has been. In this method, from the viewpoint of film-forming properties, a high concentration (30 wt%) gelatin aqueous solution containing transdalinase is coated on the polyester surface. The knives used are likely to harden overnight, and it is not considered practical to coat a highly concentrated aqueous gelatin solution. Moreover, only whether or not the film durability is dissolved in hot water at 90 is confirmed, and it is not disclosed whether or not the effect is maintained even after practical treatment such as washing.

 In order to avoid the harmful effects of using high-concentration gelatin aqueous solution, a method of immersing 3 wt% gelatin aqueous solution containing transdal yuminase in polyester (Japanese Patent Laid-Open No. Hei 9 3 7 7 3) has been proposed. The film durability is 90% only by confirming the presence or absence of dissolution in hot water, and it is not disclosed whether the effect is maintained even after practical treatment such as washing.

Although it has been shown that coating the fiber with high-concentration gelatin by these methods has the effect of improving the moisture permeability and moisture absorption of the fiber, it has poor washing durability, water absorption and moisture absorption. The fact was that it did not last. Disclosure of the invention

 The present invention has been made in view of such a current situation, and eliminates the disadvantages of the background technology, and provides a fiber having excellent strength and water absorption and washing durability with a simple and low cost. The object is to provide a method of manufacturing. As a result of intensive studies to solve the above-mentioned problems, the present inventors have found a method of using a wheat protein partial hydrolyzate and arrived at the present invention. That is, the present invention is as follows.

 (1) A processed fiber product obtained by allowing trans-daltaminase to act after attaching a wheat protein partial hydrolyzate to the fiber surface.

 (2) The processed fiber product according to (1), wherein the wheat protein partial hydrolyzate is obtained by subjecting wheat protein to an enzyme treatment, an acid treatment, or an alkaline treatment.

 (3) The processed fiber product according to (1) or (2), wherein the partial hydrolyzate of wheat protein has an average molecular weight of from 70 to 500.000.

 (4) A method for producing a processed fiber product, characterized in that after the wheat protein partial hydrolyzate is adhered to the fiber surface, koji langle gluteinase is allowed to act.

(5) The production method according to (4), wherein the wheat protein partial hydrolyzate is obtained by subjecting wheat protein to enzyme treatment, acid treatment or alkali treatment.

 (6) The production method according to (4) or (5), wherein the wheat protein partial hydrolyzate has an average molecular weight of from 700 to 500.000.

In the present invention, the wheat protein partial hydrolyzate means a product obtained by partially hydrolyzing wheat dartene protein with an enzyme, acid, alkali, etc., and is not hydrolyzed wheat protein, Protein hydrolysates that have undergone excessive hydrolysis to amino acids are not included. Commercially available enzyme-partially hydrolyzed wheat gluten protein (for example, DMV WG E80G PU) can be used as it is, or it can be prepared by degrading wheat gluten with an appropriate proteolytic enzyme. . In addition, acid partially hydrolyzed wheat barley dulten protein and al force partially hydrolyzed wheat gluten protein can also be used. The average molecular weight of the wheat protein partial hydrolyzate is preferably 7 0 0 to 50 0, 0 0 0 Da, more preferably 3, 0 0 0 to 4 0, OOOD a A range of about 5, 0 0 0 to 3 0, 0 0 0 Da is particularly preferable.

 The method for attaching the wheat protein partial hydrolyzate to the fiber surface is not particularly limited. For example, the fiber is immersed in a solution obtained by dissolving or dispersing the wheat protein partial hydrolyzate in a solvent such as water, or An example is application or spraying of a wheat protein partial hydrolyzate to the fiber. Wheat protein partial hydrolyzate may be present in either the single fiber filaments of the yarn bundles forming the fibers or in the gaps and surfaces of the staples. It only has to be fixed or coated on a bundle of single fiber filaments or staples. -Wheat protein partial hydrolyzate used when dipping the fiber in a solution in which the wheat protein partial hydrolyzate is dissolved or dispersed in a solvent such as water, or when applying or spraying the wheat protein partial hydrolyzate on the fiber The concentration of the physical solution is preferably 1 to 30 g ZL, and more preferably 3 to 10 g ZL in terms of cost and workability.

 The amount of the wheat protein partial hydrolyzate adhered to the fiber surface is preferably 0.1 to 3 g per 1 g of fiber, and more preferably 0.3 to 1 g in terms of cost and workability.

Transdalinase used in the present invention (hereinafter sometimes referred to as TG) is an acyltransferase belonging to EC 2.3.2.13, and is used in proteins and peptides. It is an enzyme that has the activity of catalyzing the acyl transfer reaction using a residue as a donor and a lysine residue as an acceptor. A variety of sources are known, such as mammals, fish, and microorganisms. The enzyme used in the present invention may be any enzyme having this activity, and any origin may be used. It may also be a recombinant enzyme. Examples thereof include those derived from microorganisms such as actinomycetes (see Japanese Patent No. 2 5 7 2 7 16) and Bacillus subtilis (see Japanese Patent No. 3 8 7 3 4 8). Also derived from guinea pig liver (see Patent No. 1 6 8 9 6 14), from microorganisms (see W0 9 6/0 6 9 3 1), bovine blood, pork blood and other animal-derived Derived from fish, salmon, red sea bream, etc. (Seki et al., Journal of the Japanese Fisheries Society, 1990, 56, 125-132), derived from oysters (US patent) No. 5 7 3 6 3 5 6)). In addition, those produced by gene recombination (for example, Japanese Patent No. 3 0 1 0 5 89, Japanese Patent Application Laid-Open No. 1 1 1 7 5 8 7 6, WO 0 1 2 3 5 9 1, WO 0 2/0 8 1 6 94 publication, WO 2 0 04Z0 7 8 9 7 3 publication), disulfide bond-introduced transdal junction minase with improved heat resistance (WO 2 0 0 8/0 9 9 8 9 8 ) Etc.

 Microbial-derived transdalase minase commercially available from Ajinomoto Co., Inc. under the trade name “Activa” TG is an example of a transglutaminase used in the present invention.

 Transdaltaminase is allowed to act by immersing the fiber in a solution containing wheat protein partial hydrolyzate and TG, or by immersing the fiber in a wheat protein partial hydrolyzate solution and then TG solution. An example of this is the method of immersing in From the viewpoint of the enzymatic reactivity and stability of TG, ρ 含 む is preferably from 4 to 12, and more preferably from 5 to 8, in the solution containing TG of the wheat protein partial hydrolyzate and TG.

 The reaction time of the enzyme is not particularly limited as long as the enzyme can act on the substrate substance. It may be very short or may be allowed to act for a long time. Minutes to 24 hours are preferred. In addition, the reaction temperature may be any temperature as long as the enzyme maintains its activity, but it is preferable to operate at a temperature of 0 to 80 as a realistic temperature.

 The optimal addition amount of TG is a solution containing a partial hydrolyzate of wheat protein and TG, or the TG concentration in the TG solution is 10 to 300 UZL, preferably 100 to 300 UZL, more preferably 100-300 UZL, is appropriate, but can be appropriately adjusted depending on the type of fiber, TG reaction time, TG reaction temperature, and the like. Even if it exceeds 3 0 0 0 UZL, there is an effect, but it is not worth the cost.

 The amount of TG added is preferably 1 to 300 U for 1 g of fiber, and preferably 1 to 300 U for 1 g of wheat protein partial hydrolyzate. It can be adjusted as appropriate.

As for enzyme activity, benzyloxycarbonyl-L-Dal Yuminuriguri The reaction was carried out using syn and hydroxylamine as substrates, and the resulting hydroxamic acid was formed into an iron complex in the presence of trichloroacetic acid, and then the absorbance at 525 nm was measured, and the amount of hydroxamic acid was determined by a calibration curve. Calculate the activity. The amount of enzyme that produces 1 mol of hydroxamic acid per minute at 37, pH 6.0 was defined as 1 U.

 The processed fiber according to the present invention refers to natural fibers such as wool, silk, and cotton, synthetic fibers such as nylon, polyester, and acrylic, and those made by blending, blending, and blending of these. Protein fibers such as wool and silk, and polyamide fibers such as naydon have a transglutaminase reaction, and their terminal amino groups are also involved in cross-linking. improves. BEST MODE FOR CARRYING OUT THE INVENTION

 The following examples further illustrate the present invention. The present invention is not limited in any way by these examples. Example 1

 The following proteins and enzymes were used in the examples.

 Evening protein

 Glutamine peptide A: Wheat dartene protein partial hydrolyzate, DM V WG E 80 G P U (average molecular weight 9, 65 50 D)

 Dartamine peptide B: Wheat dartene protein partial hydrolyzate B: D MV WGE 80 GPA (average molecular weight 6 60 0 D)

 Gelatin A: Kishida Chemical's cow-derived Al-powered gelatin

 Enzyme

 Transglutaminase (E C 2. 3. 2. 1 3)

 Enzyme origin: Actinomycetes Streptomyces, Mobaraensis

Enzyme activity: 100 g unit / g silk fabric UIS L 0803 silk 2-1 attached white fabric, double flat) Exhaust treatment was performed for 1 hour each at 40 in 100 ml of an aqueous solution containing the same amount (lg) of lumine peptide (A and B) and gelatin A, respectively. Then, after drying the silk fabric after the glutamine peptide and gelatin exhaustion treatment, in 100 ml Tris-HCl buffer (pH 7) containing 10 mg of transglutaminase (100 U / L), 40 t: 1 hour Enzyme treatment was performed and dried (as a control, TG treatment was also performed on silk fabric that was not subjected to protein exhaustion treatment).

 The tear strength of the silk fabric after the treatment was measured by the pendulum method in accordance with JIS L 1096, and the tear strength (unit: 2 uton N) in the direction of cutting the warp was measured. Further, in order to examine how much the strength of the silk fabric by the above treatment is affected by repeated washing with water, the silk fabric after the above treatment was stirred for 10 minutes with a stirrer at 40 liters of distilled water at 40 liters. The washing process was repeated 3 times. Then, after the fabric was dried, the tear strength was measured by the same method as described above.

 As shown in Table 1, all of the glutamine peptides A, B, and gelatin A that had been exhausted showed an increase in the strength of the silk fibers, but the strength was maintained after washing and the increase in strength was the largest. Was dartamine peptide A. From this, it was clarified that glutamine peptide A was firmly attached to the surface of the silk fabric by the transdal reaction. On the other hand, when gelatin is used, sufficient tear strength cannot be obtained, and the method of the present invention using a wheat protein partial hydrolyzate is compared with the method disclosed in JP-A-9-3773. It showed a remarkable effect. Table 1 Strength of silk fibers after various protein treatments

. 実施例 2

Example 2

 In order to examine the effects of protein type and molecular weight, experiments were conducted using the following proteins other than those used in Example 1.

 Protein

 Glutamine peptide C: Wheat dartene protein partial hydrolyzate, SWP 5 00 0 (molecular weight estimated from S D S—P AGE 5, 0 0 0 to 3 0, 0 0 0 D)

 Daltamin peptide D: Wheat dartene protein partial degradation product, self-made (average molecular weight 3, 0 0 0 D)

 Glutamine Peptide E: Wheat Dalten Protein Partial Degradation Product, Katayama Chemical Research Institute Dalpearl 30 (acid, alkaline hydrolysis, molecular weight 40, 0 0 0 to 50, 0 0 0 D)

 For glutamine peptide D, wheat dartene was partially hydrolyzed with a protease (Bacillus amiguchi liquifaciens MRP protein) to a mean molecular weight of 300 D. After the completion of the reaction, insoluble materials were removed, and powder was prepared by drying with spray dry.

 Take about 1 lg of silk cloth (JIS L 0803 silk 2-1 attached white cloth, Hiraha double) and gluten-min peptide (3 types) each in 40 ml in 100 ml of aqueous solution containing the same amount (lg) as the weight of the cloth. In each case, exhaustion treatment was performed for 1 hour. Then, after drying the silk fabric after the glutamine peptide exhaustion treatment, the enzyme treatment was performed in 400 ml of Tris-HCl buffer (pH 7) containing 40 mg of Transdal Yuminase (100 UZ L), 40: for 1 hour. And dried. The tear strength of the silk fabric after the treatment was measured by the pendulum method according to JIS L 1096, and the tear strength (unit: Newton N) in the direction of cutting the warp was measured.

The results are shown in Table 2 together with the results of Example 1. For glutamine peptides, an increase in tear strength was observed with an increase in average molecular weight. With regard to glutamine peptide C, an effect of smooth touch was also observed. Regarding glutamine peptide E, although it was an acid or alkaline hydrolyzate, an increase in tear strength was observed. Table 2 Effect of protein molecular weight on silk fiber strength

実施例 3

Example 3

 In order to examine the effects of the protein concentration and the transdaltaminase concentration, experiments were performed using glutamine peptide C used in Example 2. Take about 1 lg of silk fabric (JIS L 0803 silk 2-1 attached white cloth, Hiraha double) and perform exhaustion treatment for 1 hour each at 40 in 100 ml of aqueous solution containing glutamine peptide and C. It was. The silk fabric after the glutamine peptide C exhaustion treatment was dried, and then subjected to an enzyme treatment in 100 ml of Tris-HCl buffer (pH 7) containing transdalinase for 40 hours for 1 hour, followed by drying. Table 3 shows the concentrations of processed protein and transdal protein. The tear strength of the silk fabric after the treatment was measured by the pendulum method according to JIS L 1096, and the tear strength (unit: Newton N) in the direction of cutting the warp was measured.

Table 3 shows the results. With regard to protein concentration, a remarkable effect was obtained at 1 gZL or higher, and the tear strength increased significantly as the concentration increased. With regard to the transdermal concentration, a significant increase in tear strength was confirmed at all concentrations tested. Table 3 Effect of protein concentration and transdal concentration on silk fiber strength

 Protein concentration

 0.1 0.3 1 3 10 30

 (g / L)

 Enzyme activity

 1,000 1,000 1,000 1,000 1,000 1,000

 (UZL)

 Tear strength

 4.67 4.55 6.08 8.88 11.46 12.91

 (N)

Protein concentration

 10 10 10 10 0 0

 (gZL)

 Enzyme activity

 10 30 100 3,000 3,000 0

 (UZL)

 Tear strength

 12.39 10.56 11.39 11.90 4.33 4.10

 (N) Example 4

 Polyester fabric (JIS L 0803 'white fabric with polyester) lg was collected, and glutamine peptide A or gelatin A in 100 ml of an aqueous solution containing the same amount (lg) of each as the weight of the fabric at 40 ° C for 1 hour each. Exhaust treatment was performed. Then, after drying the polyester fabric after the glutamine peptide A and gelatin A exhaustion treatment, in 100 ml of Tris-HCl buffer (pH 7) containing 10 mg of transdaltaminase (10 0 UZL), 40, 1 hour, TG treatment was performed and dried (as a control, polyester fabric that had not been subjected to protein exhaustion treatment was also subjected to enzyme treatment).

The tear strength of the polyester fabric after the treatment was measured by the pendulum method according to HS L 1096, and the tear strength (unit: Newton N) in the direction of cutting the warp was measured. Further, in order to evaluate the change in surface hydrophilicity of the fabric after the treatment, a water absorption test based on the HS L 1907 dropping method was conducted. In this dripping method, the water drop infiltration area (unit: cm2) after 1 minute of water dripping was measured. further, In order to investigate how much the surface hydrophilicity of the polyester fabric by the above treatment is affected by repeated washing, the fabric after the above treatment is subjected to the JISL 0844 A-2 method (40 t: 5 g / detergent). A repeated washing test was conducted according to the conditions of stirring (42 rpm, 30 minutes). Repeated washing was performed with the detergent added for the first time and without detergent for the second time. Then, after the washed fabric was dried, the surface hydrophilicity was measured by the same method as described above.

 As shown in Table 4, the tear resistance of the polyester fabric exhausted with dartamine peptide A was improved. In addition, the polyester fabric exhausted with glutamine peptide A and gelatin A has a significantly improved surface hydrophilicity, but only the glutamine peptide retains the surface hydrophilicity after the washing test. . It was confirmed that surface hydrophilicity was improved even after washing by attaching glutamine peptide A to the polyester surface and allowing TG to act. The only drawback of polyester is that it does not absorb water (sweat), and in order to compensate for this drawback, it is often blended with cotton. It was suggested that the disadvantages of polyester could be improved.

On the other hand, when gelatin is used, sufficient tear strength and water droplet infiltration area cannot be obtained. Compared with the method disclosed in Japanese Patent Laid-Open No. Hei 9 37 7 3 The method of the invention showed a significant effect.

Table 4 Strength of polyester after various protein treatments, water drop penetration area

実施例 5

Example 5

 Nylon fabric (white cloth attached to JIS L 0803 nylon) Each lg was collected and exhausted for 1 hour each at 40 in 100 ml of an aqueous solution containing the same amount (lg) of glutamine peptide A or gelatin A as the weight of the fabric. Went. Then, after drying the nylon fabric after the glutamine peptide A and gelatin A exhaustion treatment, 100 ml of salmon squirrel containing 10 mg of transdaltaminase. In hydrochloric acid buffer (PH7) (100 UZL), 40 hours, 1 hour TG treatment was performed and dried (as a control, enzyme treatment was also applied to a knitted fabric not subjected to protein exhaustion treatment).

The tear strength of the nylon fabric after the treatment was measured by the pendulum method according to nSL 1096, and the tear strength (unit: Newton N) in the direction of cutting the warp was measured. Further, in order to evaluate the change in surface hydrophilicity of the fabric after the treatment, a water absorption test based on the HS L 1907 dropping method was conducted. In this dripping method, the water drop infiltration area (unit: cm2) 1 minute after water dripping was measured. Furthermore, in order to investigate how much the surface hydrophilicity of the nylon fabric treated by the above treatment is affected by repeated washing, the treated fabric was subjected to the JIS L 0844 A-2 method (40, detergent 5 g / A repeated washing test was conducted according to the conditions of stirring (42 rpm, 30 minutes). Repeated washing is performed with detergent added at the first time. The second time was performed without detergent. Then, after the washed fabric was dried, the surface hydrophilicity was measured by the same method as described above.

 As shown in Table 5, the nylon fabric that was exhausted with glutamine peptide A and gelatin A had improved tear strength. In addition, the polyester fabric that was exhausted with glutamine peptide A and gelatin A showed the ability to improve surface hydrophilicity. Glutamine peptide A showed a water droplet permeation area four times or more. In addition, only glutamine peptide retained surface hydrophilicity after the washing test. It was confirmed that surface hydrophilicity is improved even after washing by attaching glutamine peptide A to the nylon surface and allowing TG to act on it. On the other hand, when gelatin is used, a sufficient water droplet permeation area cannot be obtained, and the method of the present invention using a partially hydrolyzed product of wheat protein as compared with the method disclosed in JP 9-37 7 3 Showed a remarkable effect. Table 5 Nylon strength and water drop penetration area after various protein treatments

実施例 6

Example 6

Polyester fabric (JISL 0803 polyester-attached white fabric) 1. Collect 25 g of each, and for 1 hour each at 40 in 200 ml of an aqueous solution containing glutamine peptide A in the same amount (1.25 g) as the weight of the fabric. Exhaust treatment was performed. And after drying the peptide fabric after peptide exhaustion treatment, TG treatment in 200ml Tris-HCl buffer solution (pH7) containing 200mg of NAZE (1000U / L) at 40 for 1 hour, dried (as a control, protein exhaustion treatment, enzyme treatment Those that had not been subjected to protein exhaustion treatment were also carried out).

 The fabric after the above treatment was subjected to repeated washing tests according to the conditions of JISL 0844 A-2 method (40, detergent 5 g / l, stirring 42 rpm, 30 minutes). Laundry was performed under the condition that detergent was added, followed by washing under the condition without detergent and letting it air dry once. Water absorption tests based on the JIS L 1907 dropping method were performed before washing, after washing once, after washing 5 times, and after washing 10 times. In this dropping method, the water permeation area (unit: cm2) after 1 minute of water dropping was measured.

 Table 6 shows the results. Daltamin peptide A alone and not treated with transglutaminase had no effect after 5 launderings. In contrast, treatment with dartamine peptide A and transdalinase maintained the effect after 10 washes. Table 6 Effect of washing frequency on water drop surface area of TG treated polyester

 Water drop penetration area TK.

 (cm2) 1 time before 5 times after 10 times

 ― 0.94 0.94 0.94 0.94

 To Ku'Lutamin. Huh. Tide A 8.75 2.81 0.94 0.94

 To Ku'Lutamin. Huh. Chido A + TG 9.06 5.31 4.69 3.75

Example 7

 In order to investigate the effects of the concentration of protein and the concentration of transdaltaminase, experiments were conducted using glutamine peptide A.

Polyester fabric UIS L 0803 Polyester-attached white fabric) 1.25 g is collected and glutamine peptide A is equal to the weight of the fabric (1.25 g), or 10 in 40 ml of an aqueous solution containing 1 (0.125 g) of 10 minutes. Each was exhausted for 1 hour. And the polyester fabric after peptide exhaustion treatment is dried Then, TG treatment was performed for 1 hour in 40 ml (10000 UZL, 1000 U / L, respectively) in 200 ml of Tris-HCl buffer (PH7) containing 2000 rag or 200 mg of transglutaminase, Dried (as a control, a protein exhaustion treatment was performed without enzyme treatment).

 The fabric after the above treatment was subjected to repeated washing tests according to the conditions of the L 0844 A-2 method (40, detergent 5 g / stirring 42 rpm, 30 minutes). Laundry was performed under the condition that detergent was added, followed by washing under the condition without detergent and letting it air dry once. Water absorption tests based on the JIS L 1907 dropping method were performed before washing, after washing once, after washing 5 times, and after washing 10 times. In this dropping method, the water permeation area (unit: cm2) after 1 minute of water dropping was measured.

Table 7 shows the results. When the enzyme concentration was increased 10 times, the peptide concentration was reduced to 1/10, and in both cases, the effect was retained until after 10 washes.

Table 7 Effect of number of washings on water drop surface area of TG treated polyester

実施例 8

Example 8

 Polyester fabric (J IS L 0803 white fabric with polyester) 1.25g was collected and 100ml of Tris-HCl buffer (pH7) containing glutamine peptide A and transdaltaminase lOOOmg in the same amount (1.25g) as the weight of the fabric. Inside (10000U ZL), 40t: 1 hour, glutamine peptide exhaustion treatment and transglutaminase treatment were performed simultaneously.

The fabric after the above treatment was subjected to repeated washing tests according to the conditions of JISL 0844 A-2 method (40 ° C., detergent 5 g / stirring 42 rpm, 30 minutes). Laundry was performed under the condition that detergent was added, followed by washing under the condition without detergent and letting it air dry once. Water absorption tests based on the JIS L 1907 dropping method were performed before washing, after washing once, after washing 5 times, and after washing 10 times. In this dropping method, the water permeation area (unit: cm2) was measured 1 minute after dropping. Table 8 shows the results. Even when the exhaustion treatment of dartamine peptide A and the transdalinase treatment were performed at the same time, the effect was retained even after 10 washes. Table 8

 Water drop penetration area Washing | 1 li rinsing

 ¾t before rinsing

 (cm2) After 1 time After 5 times After 10 times

 To Ku'Lutamin. Huh. Chido A

 1 2.50 6.25 4.40 2.1 0

 + TG

Industrial applicability

 According to the present invention, a fiber processed product having improved strength and excellent water absorption can be obtained simply and at low cost, and is extremely useful in the textile industry.

Claims

The scope of the claims 1. A processed fiber product obtained by applying a transglutaminase after attaching a wheat protein partial hydrolyzate to the fiber surface.  2. The processed fiber product according to claim 1, wherein the wheat protein partial hydrolyzate is obtained by subjecting wheat protein to an enzyme treatment, an acid treatment or an alkali treatment.  3. The processed fiber product according to claim 1 or 2, wherein the wheat protein partial hydrolyzate has an average molecular weight of from 70 to 500.000.  4. A method for producing a processed fiber product, characterized in that after a wheat protein partial hydrolyzate is adhered to a fiber surface, transdalinase is allowed to act.  5. The method according to claim 4, wherein the wheat protein partial hydrolyzate is obtained by subjecting wheat protein to enzyme treatment, acid treatment or alkali treatment.  6. The method according to claim 4 or 5, wherein the wheat protein partial hydrolyzate has an average molecular weight of from 70 to 500.000.