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WO2009066695A1 - 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット - Google Patents

糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット Download PDF

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Publication number
WO2009066695A1
WO2009066695A1 PCT/JP2008/071023 JP2008071023W WO2009066695A1 WO 2009066695 A1 WO2009066695 A1 WO 2009066695A1 JP 2008071023 W JP2008071023 W JP 2008071023W WO 2009066695 A1 WO2009066695 A1 WO 2009066695A1
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Prior art keywords
fecal sample
nucleic acids
preparing
solution
feces
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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PCT/JP2008/071023
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English (en)
French (fr)
Inventor
Yasuo Tanigami
Tomonori Nagaoka
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Olympus Corp
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Olympus Corp
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Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Priority to CN2008801248666A priority Critical patent/CN101952460A/zh
Priority to EP08851485A priority patent/EP2218792A4/en
Priority to JP2009542570A priority patent/JP5566111B2/ja
Publication of WO2009066695A1 publication Critical patent/WO2009066695A1/ja
Priority to US12/782,537 priority patent/US8754204B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

本発明は、糞便中の大腸剥離細胞等の哺乳細胞由来の核酸等を効率よく回収することが可能な糞便試料を、煩雑な操作を必要とせずに調製し得る方法、該方法に用いられる糞便試料調製用溶液及び採便用キット、並びに、該方法により調製された糞便試料を用いて糞便中の核酸を回収し解析する方法を提供する。より具体的には糞便試料から核酸を効率よく回収するための糞便試料の調製方法であって、採取された糞便を、水溶性有機溶媒を有効成分とする糞便試料調製用溶液に混合させることを特徴とする、糞便試料の調製方法、該方法に用いられる糞便試料調製用溶液、採便用キット、該調製方法により調製された糞便試料中から、腸内常在菌由来の核酸と腸内常在菌以外の生物由来の核酸とを同時に回収することを特徴とする核酸回収方法、並びに、該核酸回収方法により回収された核酸を解析することを特徴とする核酸解析方法である。
PCT/JP2008/071023 2007-11-20 2008-11-19 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット Ceased WO2009066695A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN2008801248666A CN101952460A (zh) 2007-11-20 2008-11-19 用于制备粪便样品的方法、制备粪便样品用溶液和采集粪便用试剂盒
EP08851485A EP2218792A4 (en) 2007-11-20 2008-11-19 PROCESS FOR PREPARATION OF A KOTPROBE, SOLUTION FOR PREPARATION OF A KOTPROBE AND KOTSAMMELKIT
JP2009542570A JP5566111B2 (ja) 2007-11-20 2008-11-19 Ｒｎａ回収方法
US12/782,537 US8754204B2 (en) 2007-11-20 2010-05-18 Method for preparing stool sample, solution for preparing stool sample, and kit for collecting stool

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-300298 2007-11-20
JP2007300298 2007-11-20
JP2008-080084 2008-03-26
JP2008080084 2008-03-26

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/782,537 Continuation US8754204B2 (en) 2007-11-20 2010-05-18 Method for preparing stool sample, solution for preparing stool sample, and kit for collecting stool

Publications (1)

Publication Number Publication Date
WO2009066695A1 true WO2009066695A1 (ja) 2009-05-28

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Family Applications (1)

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PCT/JP2008/071023 Ceased WO2009066695A1 (ja) 2007-11-20 2008-11-19 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット

Country Status (5)

Country Link
US (1) US8754204B2 (ja)
EP (1) EP2218792A4 (ja)
JP (1) JP5566111B2 (ja)
CN (1) CN101952460A (ja)
WO (1) WO2009066695A1 (ja)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010024251A1 (ja) * 2008-08-26 2010-03-04 オリンパス株式会社 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット
WO2010064634A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット
WO2010064628A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 核酸含有試料の調製方法、試料調製用溶液、及び核酸の解析方法
EP2314677A4 (en) * 2008-07-23 2011-12-14 Olympus Corp METHOD OF TAKING NUCLEIC ACID FROM A CHAIR SAMPLE, NUCLEIC ACID ANALYSIS METHOD AND DEVICE FOR TREATING CHAIR SAMPLES
CN112033743A (zh) * 2020-07-16 2020-12-04 上海市东方医院（同济大学附属东方医院） 一种帕金森粪便液提取装置
JP2022535747A (ja) * 2019-05-28 2022-08-10 ケース ウエスタン リザーブ ユニバーシティ Ｄｎａのメチル化を保存するための組成物および方法

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8338130B2 (en) 2011-02-25 2012-12-25 Medical Chemical Corporation Universal fecal fixative comprising a low molecular weight alcohol, a zinc salt and an organic acid
AU2012273121B2 (en) 2011-06-19 2016-08-25 Abogen, Inc. Devices, solutions and methods for sample collection
CN106526186A (zh) 2011-09-12 2017-03-22 克里蒂科斯有限责任公司 检测靶分子的非侵入性方法
US20140363397A1 (en) * 2011-09-14 2014-12-11 Queen's University At Kingston Method for treatment of disorders of the gastrointestinal system
US9744155B2 (en) * 2012-03-28 2017-08-29 Ixcela, Inc. IPA as a therapeutic agent, as a protective agent, and as a biomarker of disease risk
IL247662B2 (en) 2014-03-07 2025-02-01 Dna Genotek Inc Composition and method for stabilizing nucleic acids in biological samples
US9528105B2 (en) 2014-09-04 2016-12-27 Techlab, Inc. Nucleic acid extraction using organic solvents to remove inhibitors
US20210146352A1 (en) * 2017-07-24 2021-05-20 Epitope Diagnostics, Inc. Fecal sample collection and analyte extraction device and method
CN107312717A (zh) * 2017-08-08 2017-11-03 苏州阿迪康生物科技有限公司 一种粪便类样本的保存方法、保存用溶液、制备方法及应用
CN108893523A (zh) * 2018-05-31 2018-11-27 厦门安捷致善医学数据科技有限公司 一种粪便样品常温保存液
CN109517883A (zh) * 2018-12-05 2019-03-26 广州市达信智能科技有限公司 一种人粪便样本中脱落细胞的保存液
US11041847B1 (en) 2019-01-25 2021-06-22 Ixcela, Inc. Detection and modification of gut microbial population
CN111826371A (zh) * 2019-04-15 2020-10-27 上海锐翌生物科技有限公司 粪便核酸保存液及其制备方法和应用
CN111044323A (zh) * 2020-03-03 2020-04-21 贵州里定医疗网络科技股份有限公司 人体大便常规检测液基预处理采样瓶
EP4158016A4 (en) 2020-05-27 2024-06-05 Case Western Reserve University COMPOSITIONS AND METHODS FOR PRESERVING DNA METHYLATION
CN115486439B (zh) * 2022-09-23 2024-12-10 深圳微辰生命科技有限公司 粪便保存方法、乙醇在制备粪便保存液及短链脂肪酸检测试剂或试剂盒中的应用

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0672837B2 (ja) 1985-12-25 1994-09-14 アンドレアス・スツアバドス ペースト状試料物質用の検査容器
JPH06265542A (ja) * 1992-07-22 1994-09-22 Yakult Honsha Co Ltd マウス腸管障害の診断方法
JPH07120460A (ja) * 1993-10-28 1995-05-12 Dainippon Printing Co Ltd 便内潜血検出装置
JPH10503384A (ja) * 1995-12-22 1998-03-31 エグザクト ラボラトリーズ， インコーポレイテッド ゲノム上不均一な細胞サンプルにおける形質転換細胞のクローン集団検出のための方法
JPH11511982A (ja) 1995-09-06 1999-10-19 メディカル・リサーチ・カウンシル 細胞を分離する方法
JP2001128662A (ja) 1999-09-10 2001-05-15 Becton Dickinson & Co 診断検査用細胞に長期安定性をもたらす組成物
JP2002511585A (ja) * 1998-04-13 2002-04-16 エグザクト サイエンシーズ コーポレイション 糞便サンプルから疾患を診断するための方法
JP2003153688A (ja) 1991-05-01 2003-05-27 Cytyc Corp 細胞保存溶液
JP2004500897A (ja) 2000-06-21 2004-01-15 ダイジーン・コーポレーション 普遍的収集培地
JP2004519202A (ja) 1999-04-15 2004-07-02 パドマナバン ピー ナイール 大腸直腸癌および他の胃腸病態の非観血的検出
JP2005532824A (ja) 2002-05-10 2005-11-04 ザユニバーシティー オブ マイアミ 細胞および組織のｒｎａおよび形態の保存

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3957436A (en) * 1974-04-29 1976-05-18 Kallestad Laboratories, Inc. Resultant color step indicator
US5998483A (en) * 1991-09-20 1999-12-07 Camiener; Gerald W. Glyoxal-containing preservative compositions
US5670325A (en) * 1996-08-14 1997-09-23 Exact Laboratories, Inc. Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample
US5741650A (en) * 1996-01-30 1998-04-21 Exact Laboratories, Inc. Methods for detecting colon cancer from stool samples
MXPA97000542A (es) * 1996-03-25 2004-05-27 Univ Mexico Nacional Autonoma Sondas de adn especificas para identificacion delas especies taenia solium y taenia saginata.
US5855913A (en) * 1997-01-16 1999-01-05 Massachusetts Instite Of Technology Particles incorporating surfactants for pulmonary drug delivery
US6020137A (en) * 1996-08-14 2000-02-01 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
US6146828A (en) * 1996-08-14 2000-11-14 Exact Laboratories, Inc. Methods for detecting differences in RNA expression levels and uses therefor
US6143529A (en) * 1996-08-14 2000-11-07 Exact Laboratories, Inc. Methods for improving sensitivity and specificity of screening assays
US6203993B1 (en) * 1996-08-14 2001-03-20 Exact Science Corp. Methods for the detection of nucleic acids
US6100029A (en) * 1996-08-14 2000-08-08 Exact Laboratories, Inc. Methods for the detection of chromosomal aberrations
US6300077B1 (en) * 1996-08-14 2001-10-09 Exact Sciences Corporation Methods for the detection of nucleic acids
US5952200A (en) * 1997-02-06 1999-09-14 University Of South Carolina Method of diagnosing cancer in human cells using a reverse transcriptase-polymerase chain reaction for identifying the presence of stromelysin-3
US20020004201A1 (en) * 1997-06-16 2002-01-10 Lapidus Stanley N. Methods for the detection of loss of heterozygosity
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
WO2000029618A1 (en) * 1998-11-12 2000-05-25 University Of Virginia Patent Foundation Non-invasive detection of helicobacter pylori infection
US6335193B1 (en) 1999-04-15 2002-01-01 Padmanabhan P Nair Isolated colonocytes
JP4599684B2 (ja) * 2000-07-26 2010-12-15 株式会社島津製作所 糞便からの核酸精製法
CN100368566C (zh) 2005-12-28 2008-02-13 中国科学院南海海洋研究所 水生动物粪样细菌dna提取试剂盒及方法
CH706214B1 (fr) 2012-03-09 2016-09-30 Sowind SA Barillet de pièce d'horlogerie.

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0672837B2 (ja) 1985-12-25 1994-09-14 アンドレアス・スツアバドス ペースト状試料物質用の検査容器
JP2003153688A (ja) 1991-05-01 2003-05-27 Cytyc Corp 細胞保存溶液
JPH06265542A (ja) * 1992-07-22 1994-09-22 Yakult Honsha Co Ltd マウス腸管障害の診断方法
JPH07120460A (ja) * 1993-10-28 1995-05-12 Dainippon Printing Co Ltd 便内潜血検出装置
JPH11511982A (ja) 1995-09-06 1999-10-19 メディカル・リサーチ・カウンシル 細胞を分離する方法
JPH10503384A (ja) * 1995-12-22 1998-03-31 エグザクト ラボラトリーズ， インコーポレイテッド ゲノム上不均一な細胞サンプルにおける形質転換細胞のクローン集団検出のための方法
JP2002511585A (ja) * 1998-04-13 2002-04-16 エグザクト サイエンシーズ コーポレイション 糞便サンプルから疾患を診断するための方法
JP2004519202A (ja) 1999-04-15 2004-07-02 パドマナバン ピー ナイール 大腸直腸癌および他の胃腸病態の非観血的検出
JP2001128662A (ja) 1999-09-10 2001-05-15 Becton Dickinson & Co 診断検査用細胞に長期安定性をもたらす組成物
JP2004500897A (ja) 2000-06-21 2004-01-15 ダイジーン・コーポレーション 普遍的収集培地
JP2005532824A (ja) 2002-05-10 2005-11-04 ザユニバーシティー オブ マイアミ 細胞および組織のｒｎａおよび形態の保存

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LIND ET AL.: "A CpG island hypermethylation profile of primary colorectal carcinomas and colon cancer cell lines", MOLECULAR CANCER, vol. 3, no. 28, 2004
NILSSON ET AL., JOURNAL OF CLINICAL MICROBIOLOGY, vol. 42, no. 8, 2004, pages 3781 - 3788

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2314677A4 (en) * 2008-07-23 2011-12-14 Olympus Corp METHOD OF TAKING NUCLEIC ACID FROM A CHAIR SAMPLE, NUCLEIC ACID ANALYSIS METHOD AND DEVICE FOR TREATING CHAIR SAMPLES
WO2010024251A1 (ja) * 2008-08-26 2010-03-04 オリンパス株式会社 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット
WO2010064634A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット
WO2010064628A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 核酸含有試料の調製方法、試料調製用溶液、及び核酸の解析方法
JP2022535747A (ja) * 2019-05-28 2022-08-10 ケース ウエスタン リザーブ ユニバーシティ Ｄｎａのメチル化を保存するための組成物および方法
CN112033743A (zh) * 2020-07-16 2020-12-04 上海市东方医院（同济大学附属东方医院） 一种帕金森粪便液提取装置
CN112033743B (zh) * 2020-07-16 2024-04-30 上海市东方医院（同济大学附属东方医院） 一种帕金森粪便液提取装置

Also Published As

Publication number Publication date
CN101952460A (zh) 2011-01-19
JP5566111B2 (ja) 2014-08-06
US20100248250A1 (en) 2010-09-30
EP2218792A1 (en) 2010-08-18
US8754204B2 (en) 2014-06-17
JPWO2009066695A1 (ja) 2011-04-07
EP2218792A4 (en) 2010-12-08

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