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WO2009052348A3 - Manipulation de billes dans des gouttelettes - Google Patents

Manipulation de billes dans des gouttelettes Download PDF

Info

Publication number
WO2009052348A3
WO2009052348A3 PCT/US2008/080264 US2008080264W WO2009052348A3 WO 2009052348 A3 WO2009052348 A3 WO 2009052348A3 US 2008080264 W US2008080264 W US 2008080264W WO 2009052348 A3 WO2009052348 A3 WO 2009052348A3
Authority
WO
WIPO (PCT)
Prior art keywords
droplet
beads
actuator
droplets
manipulation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/080264
Other languages
English (en)
Other versions
WO2009052348A2 (fr
Inventor
Ramakrishnan Sista
Vamsee Pamula
Vijay Srinivasan
Michael Pollack
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advanced Liquid Logic Inc
Original Assignee
Advanced Liquid Logic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP08840456A priority Critical patent/EP2212683A4/fr
Application filed by Advanced Liquid Logic Inc filed Critical Advanced Liquid Logic Inc
Publication of WO2009052348A2 publication Critical patent/WO2009052348A2/fr
Publication of WO2009052348A3 publication Critical patent/WO2009052348A3/fr
Priority to US12/761,147 priority patent/US8470606B2/en
Priority to US12/761,066 priority patent/US8809068B2/en
Anticipated expiration legal-status Critical
Priority to US14/308,110 priority patent/US9086345B2/en
Priority to US14/746,276 priority patent/US9377455B2/en
Priority to US14/978,935 priority patent/US9494498B2/en
Priority to US15/266,693 priority patent/US10139403B2/en
Priority to US16/191,270 priority patent/US10809254B2/en
Priority to US16/948,074 priority patent/US11789015B2/en
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B01J2219/00466—Beads in a slurry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00655—Making arrays on substantially continuous surfaces the compounds being bound to magnets embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention porte sur un procédé de dispersion ou de circulation de billes magnétiquement sensibles à l'intérieur d'une gouttelette dans un actionneur de gouttelettes. Selon un mode de réalisation, l'invention utilise un actionneur de gouttelettes avec une pluralité d'électrodes à opérations de gouttelettes configurées pour transporter la gouttelette, et un champ magnétique présent au niveau d'une partie de la pluralité d'électrodes d'opérations de gouttelettes. Une gouttelette contenant une bille est disposée sur l'actionneur de gouttelettes en présence du champ magnétique uniforme. Les billes sont amenées à circuler dans la gouttelette pendant l'incubation par la conduite d'opérations de gouttelettes sur la gouttelette à l'intérieur d'une région uniforme du champ magnétique. D'autres modes de réalisation sont également présentés.
PCT/US2008/080264 2006-04-18 2008-10-17 Manipulation de billes dans des gouttelettes Ceased WO2009052348A2 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
EP08840456A EP2212683A4 (fr) 2007-10-17 2008-10-17 Manipulation de billes dans des gouttelettes
US12/761,147 US8470606B2 (en) 2006-04-18 2010-04-15 Manipulation of beads in droplets and methods for splitting droplets
US12/761,066 US8809068B2 (en) 2006-04-18 2010-04-15 Manipulation of beads in droplets and methods for manipulating droplets
US14/308,110 US9086345B2 (en) 2006-04-18 2014-06-18 Manipulation of beads in droplets and methods for manipulating droplets
US14/746,276 US9377455B2 (en) 2006-04-18 2015-06-22 Manipulation of beads in droplets and methods for manipulating droplets
US14/978,935 US9494498B2 (en) 2006-04-18 2015-12-22 Manipulation of beads in droplets and methods for manipulating droplets
US15/266,693 US10139403B2 (en) 2006-04-18 2016-09-15 Manipulation of beads in droplets and methods for manipulating droplets
US16/191,270 US10809254B2 (en) 2006-04-18 2018-11-14 Manipulation of beads in droplets and methods for manipulating droplets
US16/948,074 US11789015B2 (en) 2006-04-18 2020-09-01 Manipulation of beads in droplets and methods for manipulating droplets

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98078207P 2007-10-17 2007-10-17
US60/980,782 2007-10-17

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US11/639,531 Continuation-In-Part US8613889B2 (en) 2006-04-13 2006-12-15 Droplet-based washing

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US12/761,066 Continuation US8809068B2 (en) 2006-04-18 2010-04-15 Manipulation of beads in droplets and methods for manipulating droplets
US12/761,066 Continuation-In-Part US8809068B2 (en) 2006-04-18 2010-04-15 Manipulation of beads in droplets and methods for manipulating droplets
US12/761,147 Continuation US8470606B2 (en) 2006-04-18 2010-04-15 Manipulation of beads in droplets and methods for splitting droplets

Publications (2)

Publication Number Publication Date
WO2009052348A2 WO2009052348A2 (fr) 2009-04-23
WO2009052348A3 true WO2009052348A3 (fr) 2009-07-30

Family

ID=40568074

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/080264 Ceased WO2009052348A2 (fr) 2006-04-18 2008-10-17 Manipulation de billes dans des gouttelettes

Country Status (2)

Country Link
EP (1) EP2212683A4 (fr)
WO (1) WO2009052348A2 (fr)

Families Citing this family (61)

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US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
EP2570812B1 (fr) 2007-02-09 2018-07-18 Advanced Liquid Logic, Inc. Procédé d'assemblage d'un dispositif actionneurs de gouttelettes
EP2109774B1 (fr) 2007-02-15 2018-07-04 Advanced Liquid Logic, Inc. Détection de capacité sur un actuateur goutte
WO2011084703A2 (fr) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Analyses d'enzymes sur un diffuseur à gouttelettes
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US9631244B2 (en) 2007-10-17 2017-04-25 Advanced Liquid Logic, Inc. Reagent storage on a droplet actuator
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US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
EP2286228B1 (fr) 2008-05-16 2019-04-03 Advanced Liquid Logic, Inc. Dispositifs et procédés actionneurs de gouttelettes pour manipuler des billes
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
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WO2011137533A1 (fr) 2010-05-05 2011-11-10 The Governing Council Of The University Of Toronto Procédé de traitement d'échantillons séchés utilisant un dispositif microfluidique numérique
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US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
CA2833897C (fr) 2011-05-09 2020-05-19 Advanced Liquid Logic, Inc. Retroaction microfluidique utilisant une detection d'impedance
EP2707724A4 (fr) 2011-05-10 2015-01-21 Advanced Liquid Logic Inc Concentration d'enzymes et dosages
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US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
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US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
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BR112014032727B1 (pt) 2012-06-27 2021-12-14 Illumina France Método e sistema para realizar operações de gotícula em uma gotícula em um atuador de gotículas para redução da formação de bolhas
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CN119158636A (zh) 2019-04-08 2024-12-20 米罗库鲁斯公司 多盒式数字微流控装置和使用方法
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US20070243634A1 (en) * 2006-04-18 2007-10-18 Pamula Vamsee K Droplet-based surface modification and washing
WO2008098236A2 (fr) * 2007-02-09 2008-08-14 Advanced Liquid Logic, Inc. Dispositifs actionneurs de gouttelettes et procédés employant des perles magnétiques

Non-Patent Citations (5)

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Title
MITSUHIRO SHIKIDA ET AL.: "Using wettability and interfacial tension to handle droplets of magnetic beads in a micro- chemical-analysis system", SENSORS AND ACTUATORS B, vol. 113, 8 March 2005 (2005-03-08), pages 563 - 569, XP025111760 *
NICOLE WEAVER: "Application of Magnetic Microspheres to Pyrosequencing on a Digital Microfluidic Platform", ACADEMIC YEAR 2004-2005, 31 December 2005 (2005-12-31), pages 1 - 11, XP002485691, Retrieved from the Internet <URL:http://old.ee.duke.edu/undergrads/independent_study.php> *
See also references of EP2212683A4 *
YIZHONG WANG ET AL.: "Efficient in-droplet separation of magnetic particles for digital microfluidics", J. MICROMECH. MICROENG, vol. 17, 21 September 2007 (2007-09-21), pages 2148 - 2156, XP020119955 *
YVES FOUILLET ET AL.: "Digital microfluidic design and optimization of classic and new fluidic functions for lab on a chip systems", MICROFLUID NANOFLUID, vol. 4, 20 March 2007 (2007-03-20), pages 159 - 165, XP019568462 *

Also Published As

Publication number Publication date
EP2212683A2 (fr) 2010-08-04
EP2212683A4 (fr) 2011-08-31
WO2009052348A2 (fr) 2009-04-23

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