[go: up one dir, main page]

WO2009050498A3 - Protéine - Google Patents

Protéine Download PDF

Info

Publication number
WO2009050498A3
WO2009050498A3 PCT/GB2008/003573 GB2008003573W WO2009050498A3 WO 2009050498 A3 WO2009050498 A3 WO 2009050498A3 GB 2008003573 W GB2008003573 W GB 2008003573W WO 2009050498 A3 WO2009050498 A3 WO 2009050498A3
Authority
WO
WIPO (PCT)
Prior art keywords
membrane protein
prokaryotic cells
chimeric constructs
membrane expression
chimeric membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2008/003573
Other languages
English (en)
Other versions
WO2009050498A2 (fr
Inventor
Bonnie Ann Wallace
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BIRKBECK
Original Assignee
BIRKBECK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BIRKBECK filed Critical BIRKBECK
Publication of WO2009050498A2 publication Critical patent/WO2009050498A2/fr
Publication of WO2009050498A3 publication Critical patent/WO2009050498A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne une molécule d'acide nucléique isolée codant pour une protéine membranaire chimère. La protéine membranaire chimère comprend au moins deux segments transmembranaires N-terminaux d'une protéine membranaire procaryote, tandis que les segments transmembranaires restants sont eucaryotes. L'invention concerne également une protéine membranaire chimère. La molécule d'acide nucléique codant pour la protéine membranaire chimère peut être insérée dans un organisme procaryote, par exemple dans une bactérie comme E. coli. Cela peut être fait en introduisant un vecteur dans l'organisme procaryote. La protéine membranaire chimère encodée par la molécule d'acide nucléique peut ensuite s'exprimer dans l'organisme procaryote.
PCT/GB2008/003573 2007-10-19 2008-10-20 Protéine Ceased WO2009050498A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0720563.6 2007-10-19
GB0720563A GB0720563D0 (en) 2007-10-19 2007-10-19 Protein

Publications (2)

Publication Number Publication Date
WO2009050498A2 WO2009050498A2 (fr) 2009-04-23
WO2009050498A3 true WO2009050498A3 (fr) 2009-06-04

Family

ID=38814197

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/003573 Ceased WO2009050498A2 (fr) 2007-10-19 2008-10-20 Protéine

Country Status (2)

Country Link
GB (1) GB0720563D0 (fr)
WO (1) WO2009050498A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0823366D0 (en) * 2008-12-22 2009-01-28 Uni I Oslo Synthesis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003040323A2 (fr) * 2001-11-08 2003-05-15 Children's Medical Center Corporation Canal ionique bacterien et technique de recherche de modulateurs du canal ionique
US20050202539A1 (en) * 2004-03-11 2005-09-15 Hydra Biosciences System for screening eukaryotic membrane proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003040323A2 (fr) * 2001-11-08 2003-05-15 Children's Medical Center Corporation Canal ionique bacterien et technique de recherche de modulateurs du canal ionique
US20050202539A1 (en) * 2004-03-11 2005-09-15 Hydra Biosciences System for screening eukaryotic membrane proteins

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 2 September 2003 (2003-09-02), "Bacterial voltage-sensitive ion-selective channel protein NaChBac.", XP002516535, retrieved from EBI accession no. GSP:ABR55917 Database accession no. ABR55917 *
FU D ET AL: "Structure of a glycerol-conducting channel and the basis for its selectivity", SCIENCE 2000 US, vol. 290, no. 5491, 2000, pages 481 - 486, ISSN: 0036-8075 *
IRENE NEOPHYTOU ET AL: "Eukaryotic integral membrane protein expression utilizing the Escherichia coli glycerol-conducting channel protein (GlpF)", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 77, no. 2, 9 September 2007 (2007-09-09), pages 375 - 381, XP019560704, ISSN: 1432-0614 *
LAAGE R ET AL: "Strategies for prokaryotic expression of eukaryotic membrane proteins", TRAFFIC, MUNKSGAARD, DK, vol. 2, no. 2, 1 February 2001 (2001-02-01), pages 99 - 104, XP009023195, ISSN: 1398-9219 *
LU ZHE ET AL: "Ion conduction pore is conserved among potassium channels", NATURE, vol. 413, 25 October 2001 (2001-10-25), pages 809 - 813, XP002517880, ISSN: 0006-3495 *

Also Published As

Publication number Publication date
GB0720563D0 (en) 2007-11-28
WO2009050498A2 (fr) 2009-04-23

Similar Documents

Publication Publication Date Title
WO2011044279A3 (fr) Systèmes microbiens pour la production de produits chimiques d'usage courant
WO2009011258A1 (fr) Procédé permettant de maintenir de manière stable un gène étranger dans une cellule
WO2009085935A3 (fr) Polypeptides présentant une activité cellulolytique et polynucléotides codant pour ceux-ci
WO2009085859A3 (fr) Polypeptides présentant une activité d'activation cellulolytique et polynucléotides codant pour ceux-ci
WO2009085864A3 (fr) Polypeptides présentant une activité d'activation cellulolytique et polynucléotides codant pour ceux-ci
EP3805348A3 (fr) Polypeptides avant une activite d activation cellulolytique et polynucleotides codant pour ceux-ci
WO2007109441A3 (fr) Polypeptides ayant ue activité endoglucanase et polynucléotides codant pour les polypeptides
WO2007115201A3 (fr) Polypeptides ayant une activité endoglucanase et polynucléotides encodant lesdits polypeptides
WO2009105141A3 (fr) Polypeptides ayant une activité endoglucanase et polynucléotides codant pour ceux-ci
WO2008066931A3 (fr) Chromosome de bacillus licheniformis
WO2011005867A8 (fr) Polypeptides ayant une activité cellulolytique améliorée et polynucléotides codant pour ceux-ci
MX2009012846A (es) Polipeptidos que tienen actividad celulosica mejorada y polinucleotidos que los codifican.
WO2006007373A3 (fr) Polynucleotides codant des proteines ahasl matures aux fins de creation de vegetaux tolerants a l'imidazolinone
WO2005121331A3 (fr) Polypeptides galnact2 tronques et acides nucleiques
WO2005001064A3 (fr) Polypeptides a activite alpha-amylase et polynucleotides codant pour ceux-ci
WO2009061413A3 (fr) Reporteur de fusion hybride et ses utilisations
WO2009034190A3 (fr) Marqueur d'affinité
WO2007096192A3 (fr) Production de protéines biologiquement actives
WO2010082804A3 (fr) Méthode de production d'une protéine ou d'un peptide physiologiquement actif à l'aide d'un fragment d'immunoglobuline
WO2008104890A3 (fr) Compositions et procédés de production d'apolipoprotéine
GB201201178D0 (en) Novel enzymes
EP4253534A3 (fr) Variants d'alpha-amylase et polynucléotides les codant
WO2013119302A3 (fr) Variants du polypeptide gh61 et polynucléotides codant pour ceux-ci
WO2005111217A3 (fr) Molecules d’acide nucleique de nicotiana et leurs utilisations
WO2011153276A3 (fr) Enzymes cellobiohydrolases chimères fonctionnelles et stables de classe i

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08839501

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08839501

Country of ref document: EP

Kind code of ref document: A2