[go: up one dir, main page]

WO2009047189A3 - Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien - Google Patents

Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien Download PDF

Info

Publication number
WO2009047189A3
WO2009047189A3 PCT/EP2008/063171 EP2008063171W WO2009047189A3 WO 2009047189 A3 WO2009047189 A3 WO 2009047189A3 EP 2008063171 W EP2008063171 W EP 2008063171W WO 2009047189 A3 WO2009047189 A3 WO 2009047189A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
red
light
fluorescent light
wavelength
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2008/063171
Other languages
English (en)
French (fr)
Other versions
WO2009047189A2 (de
Inventor
Stefan W. Hell
Brian Rankin
Robert Kellner
Jaydev Jethwa
Thorsten Staudt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften
Priority to EP08837241A priority Critical patent/EP2195612A2/de
Priority to JP2010527448A priority patent/JP2011503525A/ja
Publication of WO2009047189A2 publication Critical patent/WO2009047189A2/de
Publication of WO2009047189A3 publication Critical patent/WO2009047189A3/de
Priority to US12/753,349 priority patent/US8039815B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]
    • G01N2021/655—Stimulated Raman
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
    • H01S3/00—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
    • H01S3/30—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range using scattering effects, e.g. stimulated Brillouin or Raman effects
    • H01S3/302—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range using scattering effects, e.g. stimulated Brillouin or Raman effects in an optical fibre

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

Zum fluoreszenzlichtmikroskopischen Messen einer Probe (2) wird ein Fluoreszenzfarbstoff in der Probe (2) mit Licht (8) einer bestimmter Wellenlänge von einem Zustand in einen anderen Zustand überführt, wobei Licht (10) einer anderen Wellenlänge mit einer solchen Intensität in eine derart ausgewählte Lichtleiterfaser (13) eingekoppelt wird, dass in der Lichtleiterfaser (13) Raman-Streuung in solchem Ausmaß stimuliert wird, dass ein aus der Lichtleiterfaser (13) austretendes Lichtspektrum neben der eingekoppelten Wellenlänge mindestens eine rotverschobene Stokes-Linien (19 bis 28) aufweist, deren Intensitätshalbwertsbreite kleiner als ihr halber Abstand zu der er ihr im Blauen benachbarten Linie (18 bis 28) des Lichtspektrums (17) ist, und wobei die eine Wellenlänge aus einer der rotverschobenen Stokes-Linien (19 bis 28) ausgewählt wird; und Fluoreszenzlicht (6) von der Probe (2) wird ortsauflösend gemessen.
PCT/EP2008/063171 2007-10-05 2008-10-01 Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien Ceased WO2009047189A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08837241A EP2195612A2 (de) 2007-10-05 2008-10-01 Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien
JP2010527448A JP2011503525A (ja) 2007-10-05 2008-10-01 赤方偏移したストローク線を用いる、試料の蛍光顕微鏡測定法
US12/753,349 US8039815B2 (en) 2007-10-05 2010-04-02 Fluorescent light microscope for measuring a sample using red-shifted stokes lines

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007048135.9 2007-10-05
DE102007048135A DE102007048135B4 (de) 2007-10-05 2007-10-05 Fluoreszenzlichtmikroskopisches Messen einer Probe mit rotverschobenen Stokes-Linien

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/753,349 Continuation US8039815B2 (en) 2007-10-05 2010-04-02 Fluorescent light microscope for measuring a sample using red-shifted stokes lines

Publications (2)

Publication Number Publication Date
WO2009047189A2 WO2009047189A2 (de) 2009-04-16
WO2009047189A3 true WO2009047189A3 (de) 2009-09-03

Family

ID=40262141

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/063171 Ceased WO2009047189A2 (de) 2007-10-05 2008-10-01 Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien

Country Status (5)

Country Link
US (1) US8039815B2 (de)
EP (1) EP2195612A2 (de)
JP (1) JP2011503525A (de)
DE (1) DE102007048135B4 (de)
WO (1) WO2009047189A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202009007250U1 (de) 2009-05-20 2009-11-26 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Feldveränderungsmittel zur Erzeugung komplementärer Lichtintensitätsmuster
DE102009056058B4 (de) * 2009-11-25 2021-08-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung zur Erzeugung von Anregungsstrahlung für selbstkalibrierende faseroptische Raman-Sensoren, Messeinrichtung zum Erfassen der Temperatur eines Messobjekts und Raman-Sensoranordnung
DE102010037190B4 (de) * 2010-08-27 2015-11-26 Leica Microsystems Cms Gmbh Vorrichtung zum zeitlichen Verschieben von Weißlichtlaserpulsen
FR2966292B1 (fr) * 2010-10-18 2013-01-18 Centre Nat Rech Scient Methode et dispositif d'emission laser pour l'analyse spectroscopique d'un echantillon
JP2012237714A (ja) * 2011-05-13 2012-12-06 Sony Corp 非線形ラマン分光装置、顕微分光装置及び顕微分光イメージング装置
CN102564642B (zh) * 2012-02-21 2013-08-07 中国计量学院 融合拉曼放大效应的光纤拉曼频移器的全分布光纤传感器
KR20150088879A (ko) * 2012-11-28 2015-08-03 트러스티스 오브 프린스턴 유니버시티 코히렌트성 반 스토크스 라만 분광법을 사용하는 검출 시스템 및 방법
DE202013006817U1 (de) * 2013-07-30 2014-10-31 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Gepulste Laserlichtquelle für die Fluoreszenzanregung
JP6103008B2 (ja) * 2015-09-09 2017-03-29 ソニー株式会社 非線形ラマン分光装置、顕微分光装置及び顕微分光イメージング装置
JP6729878B2 (ja) * 2016-04-05 2020-07-29 ウシオ電機株式会社 多光子励起用スーパーコンティニウム光生成光源、多光子励起用スーパーコンティニウム光生成方法、多光子励起蛍光顕微鏡及び多光子励起方法
DE102021128556A1 (de) 2021-11-03 2023-05-04 Amphos GmbH STED-Mikroskop
DE102021005684A1 (de) 2021-11-16 2023-05-17 Jörn Volkher Wochnowski STED-Verfahren mit Hohllichtwellenleitern

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1118904A1 (de) * 1998-09-29 2001-07-25 Japan Science and Technology Corporation Vorrichtung zur erzeugung kurzer lichtpulse mit variabler wellenlänge und ein verfahren
DE10124983A1 (de) * 2000-05-23 2002-03-07 Imra America Inc Modulare hochenergetische breit abstimmbare ultraschnelle Faserquelle
US20030011765A1 (en) * 2001-07-03 2003-01-16 Xie Xiaoliang Sunney System and method for polarization coherent anti-stokes raman scattering microscopy
EP1288705A2 (de) * 2001-08-28 2003-03-05 Nippon Telegraph and Telephone Corporation Wellenlängenverstimmbare Pulslichtquelle
WO2004077142A1 (de) * 2003-02-25 2004-09-10 Toptica Photonics Ag Erzeugung abstimmbarer picosekunden-lichtimpulse im sichtbaren spektralbereich
US20050238070A1 (en) * 2004-03-25 2005-10-27 Gennady Imeshev Optical parametric amplification, optical parametric generation, and optical pumping in optical fibers systems

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5491344A (en) * 1993-12-01 1996-02-13 Tufts University Method and system for examining the composition of a fluid or solid sample using fluorescence and/or absorption spectroscopy
DE10012881B4 (de) * 2000-03-16 2008-09-04 Nokia Siemens Networks Gmbh & Co.Kg Ramanverstärkeranordnung
US7190705B2 (en) * 2000-05-23 2007-03-13 Imra America. Inc. Pulsed laser sources
DE20122791U1 (de) 2000-06-17 2007-11-29 Leica Microsystems Cms Gmbh Scanmikroskop
US6818907B2 (en) * 2000-10-17 2004-11-16 The President And Fellows Of Harvard College Surface plasmon enhanced illumination system
EP1378034B1 (de) * 2001-04-11 2005-06-29 The University Of Southampton Quellen und verfahren zur erzeugung von optischen pulsen
US7224518B2 (en) * 2003-02-25 2007-05-29 Toptica Photonics Ag Fiber-optic amplification of light pulses
US7064824B2 (en) * 2003-04-13 2006-06-20 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. High spatial resoulution imaging and modification of structures
JP2005084266A (ja) * 2003-09-05 2005-03-31 Kawasaki Heavy Ind Ltd 光制御装置および光制御方法
DE10347712A1 (de) * 2003-10-14 2005-05-12 Leica Microsystems Rastermikroskop
DE102005020003B4 (de) 2005-04-27 2007-10-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Fluoreszenzmikroskop

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1118904A1 (de) * 1998-09-29 2001-07-25 Japan Science and Technology Corporation Vorrichtung zur erzeugung kurzer lichtpulse mit variabler wellenlänge und ein verfahren
DE10124983A1 (de) * 2000-05-23 2002-03-07 Imra America Inc Modulare hochenergetische breit abstimmbare ultraschnelle Faserquelle
US20030011765A1 (en) * 2001-07-03 2003-01-16 Xie Xiaoliang Sunney System and method for polarization coherent anti-stokes raman scattering microscopy
EP1288705A2 (de) * 2001-08-28 2003-03-05 Nippon Telegraph and Telephone Corporation Wellenlängenverstimmbare Pulslichtquelle
WO2004077142A1 (de) * 2003-02-25 2004-09-10 Toptica Photonics Ag Erzeugung abstimmbarer picosekunden-lichtimpulse im sichtbaren spektralbereich
US20050238070A1 (en) * 2004-03-25 2005-10-27 Gennady Imeshev Optical parametric amplification, optical parametric generation, and optical pumping in optical fibers systems

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BENABID F ET AL: "Compact, stable and efficient all-fibre gas cells using hollow-core photonic crystal fibres", NATURE, vol. 434, no. 7032, 24 March 2005 (2005-03-24), NATURE PUBLISHING GROUP, LONDON, UK, pages 488 - 491, XP002492113, ISSN: 0028-0836 *
BENABID F ET AL: "Stimulated Raman scattering in hydrogen-filled hollow-core photonic crystal fiber", SCIENCE, vol. 298, no. 5592, 11 October 2002 (2002-10-11), AMERICAN ASSOC. ADV. SCI USA, pages 399 - 402, XP002529809, ISSN: 0036-8075 *
BENABID F ET AL: "Ultrahigh efficiency laser wavelength conversion in a gas-filled hollow core photonic Crystal fiber by pure stimulated rotational Raman scattering in molecular hydrogen", PHYSICAL REVIEW LETTERS, vol. 93, no. 12, 17 September 2004 (2004-09-17), APS, USA, pages 123903/1 - 123903/4, XP002529808, ISSN: 0031-9007 *
BEWERSDORF J ET AL: "Picosecond pulsed two-photon imaging with repetition rates of 200 and 400 MHz", JOURNAL OF MICROSCOPY, vol. 191, 1 July 1998 (1998-07-01), BLACKWELL SCIENCE FOR R. MICROSC. SOC UK, pages 28 - 38, XP002529810, ISSN: 0022-2720 *
GARZÓN R J ET AL: "Chromatic confocal microscopy by means of continuum light generated through a standard single-mode fibre; Chromatic confocal microscopy by means of continuum light generated through a standard single-mode fibre", JOURNAL OF OPTICS. A, PURE AND APPLIED OPTICS, vol. 6, no. 6, 1 June 2004 (2004-06-01), INSTITUTE OF PHYSICS PUBLISHING, BRISTOL, GB, pages 544 - 548, XP020081462, ISSN: 1464-4258 *
MUSSOT A ET AL: "Generation of a broadband single-mode supercontinuum in a conventional dispersion-shifted fiber by use of a subnanosecond microchip laser", OPTICS LETTERS, vol. 28, no. 19, 1 November 2003 (2003-11-01), OPT. SOC. AMERICA USA, pages 1820 - 1822, XP002529811, ISSN: 0146-9592 *

Also Published As

Publication number Publication date
US20100187438A1 (en) 2010-07-29
DE102007048135B4 (de) 2012-02-16
JP2011503525A (ja) 2011-01-27
EP2195612A2 (de) 2010-06-16
US8039815B2 (en) 2011-10-18
DE102007048135A1 (de) 2009-04-16
WO2009047189A2 (de) 2009-04-16

Similar Documents

Publication Publication Date Title
WO2009047189A3 (de) Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien
EA201000526A1 (ru) Спектрометр со светодиодной матрицей
WO2009033021A3 (en) Light source with wavelength converting phosphor
WO2008058058A3 (en) Apparatus and method for estimating filtrate contamination in a formation fluid
JP2009222531A5 (de)
WO2009066264A3 (en) Multi-spectral light source illumination and fluorescence detection
EP2086074A3 (de) Impulslaservorrichtung, Terahertz-Messvorrichtung und Terahertz-Tomographievorrichtung
WO2007127356A3 (en) Pulsed uv and visible raman laser systems
EP1959247A3 (de) Spektralmessungsvorrichtung und -messverfahren unter Verwendung der Brillouin-Streuung
CN101371128A (zh) 分析装置、真伪判定装置、真伪判定方法及地下探测方法
GB2464414A (en) Distributed sensing in an optical fiber using brillouin scattering
WO2009009198A3 (en) Sers nanotag assays
TW200720699A (en) White light-emitting device using fluorescent fiber
WO2006055735A3 (en) Scanner having spatial light modulator
WO2006134103A8 (de) Verfahren und vorrichtung zur erzeugung und detektion eines raman-spektrums
GB201102640D0 (en) System and method for detecting damage to a wind turbine blade
HK1207151A1 (en) Emission device for emitting a light beam of controlled spectrum
WO2015052590A3 (en) System and method for detecting crude oil or gas underground using light scattering spectral analyses
TW200614864A (en) Organic electroluminescent display
ATE413627T1 (de) Vorrichtung zum erzeugen von polychromatischem licht mit kontinuierlichem spektrum
GB2489157A (en) Methods for allocating commingled oil production
WO2007120319A3 (en) Raman spectroscopic apparatus utilizing internal grating stabilized semiconductor laser with high spectral brightness
TW200710532A (en) Multi-bandpass filter for a projection device
WO2011023890A3 (fr) Dispositif et procédé de mesure à fibre optique
DE602008002935D1 (de) Ilchen mittels raman-spektroskopie

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08837241

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2010527448

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008837241

Country of ref document: EP