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WO2009046125A3 - Floating electrode optoelectronic tweezers (feoet) for manipulatiing oil-immersed droplets - Google Patents

Floating electrode optoelectronic tweezers (feoet) for manipulatiing oil-immersed droplets Download PDF

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Publication number
WO2009046125A3
WO2009046125A3 PCT/US2008/078476 US2008078476W WO2009046125A3 WO 2009046125 A3 WO2009046125 A3 WO 2009046125A3 US 2008078476 W US2008078476 W US 2008078476W WO 2009046125 A3 WO2009046125 A3 WO 2009046125A3
Authority
WO
WIPO (PCT)
Prior art keywords
droplets
photoconductive layer
feoet
electrodes
oil
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/078476
Other languages
French (fr)
Other versions
WO2009046125A2 (en
Inventor
Sungyong Park
Pei-Yu Chiou
Chenlu Pan
Michael A Teitell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2009046125A2 publication Critical patent/WO2009046125A2/en
Publication of WO2009046125A3 publication Critical patent/WO2009046125A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/02—Electrostatic separation of liquids from liquids

Landscapes

  • Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Manipulator (AREA)

Abstract

A floating electrode optoelectronic tweezers (FEOET) device is described for optical manipulation of oil-immersed droplets on a featureless photoconductive layer in response to the movement of optical images, and more preferably pairs of optical images retained in sufficient proximity for trapping droplets. In one embodiment, electrodes are attached to a photoconductive layer adapted with a chamber for retaining a liquid medium and aqueous droplets. A sufficient voltage bias is applied across the electrodes, and a 2D light pattern is directed to the photoconductive layer to form virtual electrodes which direct aqueous droplet motions through dielectrophoresis (DEP). The aqueous droplets contain chemical and biochemical contents which can be manipulated, such as being trapped, transported in 2D, merged, coalesced, delivered to wells or other structures. This droplet-based microfluidic system has wide applicability, such as in high throughput chemical and biological screening.
PCT/US2008/078476 2007-10-02 2008-10-01 Floating electrode optoelectronic tweezers (feoet) for manipulatiing oil-immersed droplets Ceased WO2009046125A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97689007P 2007-10-02 2007-10-02
US60/976,890 2007-10-02

Publications (2)

Publication Number Publication Date
WO2009046125A2 WO2009046125A2 (en) 2009-04-09
WO2009046125A3 true WO2009046125A3 (en) 2009-06-04

Family

ID=40526944

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/078476 Ceased WO2009046125A2 (en) 2007-10-02 2008-10-01 Floating electrode optoelectronic tweezers (feoet) for manipulatiing oil-immersed droplets

Country Status (1)

Country Link
WO (1) WO2009046125A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130319861A1 (en) * 2012-05-30 2013-12-05 Berkeley Lights, Inc. Outputting A Droplet Of Liquid Medium From A Device For Processing Micro-Objects In The Medium
CN105486867A (en) * 2014-09-19 2016-04-13 中国科学院沈阳自动化研究所 Method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force
TWI698282B (en) 2014-12-05 2020-07-11 加州大學董事會 Substrate, microfluidic device , method of moving a droplet in a microfluidic device and process for manipulating a droplet in a microfluidic device
WO2016172454A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Microfluidic cell structure
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
CN109689213B (en) 2016-05-26 2022-10-14 伯克利之光生命科技公司 Covalently modified surface, kit, preparation method and use
GB202109967D0 (en) * 2021-07-09 2021-08-25 Lightcast Discovery Ltd Improvements in or relating to imaging microdroplets in a microfluidic device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6734436B2 (en) * 2001-08-07 2004-05-11 Sri International Optical microfluidic devices and methods
WO2005100541A2 (en) * 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6734436B2 (en) * 2001-08-07 2004-05-11 Sri International Optical microfluidic devices and methods
WO2005100541A2 (en) * 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns

Also Published As

Publication number Publication date
WO2009046125A2 (en) 2009-04-09

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