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WO2009045550A3 - Système permettant de capturer et de modifier de grands morceaux d'adn génomique, et de construire des organismes comprenant des chloroplastes synthétiques - Google Patents

Système permettant de capturer et de modifier de grands morceaux d'adn génomique, et de construire des organismes comprenant des chloroplastes synthétiques Download PDF

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Publication number
WO2009045550A3
WO2009045550A3 PCT/US2008/011540 US2008011540W WO2009045550A3 WO 2009045550 A3 WO2009045550 A3 WO 2009045550A3 US 2008011540 W US2008011540 W US 2008011540W WO 2009045550 A3 WO2009045550 A3 WO 2009045550A3
Authority
WO
WIPO (PCT)
Prior art keywords
genomes
capturing
genomic dna
large pieces
minimal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/011540
Other languages
English (en)
Other versions
WO2009045550A2 (fr
Inventor
Mike Mendez
Bryan O'neill
Kari Mikkelson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sapphire Energy Inc
Original Assignee
Sapphire Energy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to KR1020107008929A priority Critical patent/KR101389254B1/ko
Priority to CA2698627A priority patent/CA2698627A1/fr
Priority to CN2008801097736A priority patent/CN101939423A/zh
Priority to JP2010528006A priority patent/JP5666908B2/ja
Priority to NZ583682A priority patent/NZ583682A/en
Priority to DK08836521.8T priority patent/DK2195426T3/da
Priority to AU2008307471A priority patent/AU2008307471B2/en
Priority to MX2010003157A priority patent/MX2010003157A/es
Application filed by Sapphire Energy Inc filed Critical Sapphire Energy Inc
Priority to EP08836521.8A priority patent/EP2195426B1/fr
Publication of WO2009045550A2 publication Critical patent/WO2009045550A2/fr
Publication of WO2009045550A3 publication Critical patent/WO2009045550A3/fr
Priority to IL204250A priority patent/IL204250A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'analyse fonctionnelle des gènes requiert fréquemment la manipulation de grandes régions génomiques. L'invention porte sur un vecteur navette levure-bactérie qui peut être utilisé pour cloner de grandes régions de l'ADN par recombinaison homologue. L'invention se rapporte également à un procédé qui permet d'isoler des génomes entiers, y compris des génomes de chloroplastes, ou de grandes parties de ces derniers, et de les manipuler. L'invention concerne aussi des procédés qui permettent de déterminer des génomes minimaux, des exigences de voies minimales et des génomes d'organelles minimaux.
PCT/US2008/011540 2007-10-05 2008-10-06 Système permettant de capturer et de modifier de grands morceaux d'adn génomique, et de construire des organismes comprenant des chloroplastes synthétiques Ceased WO2009045550A2 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
AU2008307471A AU2008307471B2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts
CN2008801097736A CN101939423A (zh) 2007-10-05 2008-10-06 用于捕获和修饰基因组dna大片段和用合成叶绿体构建生物体的系统
JP2010528006A JP5666908B2 (ja) 2007-10-05 2008-10-06 ゲノムｄｎａの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系
NZ583682A NZ583682A (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts
DK08836521.8T DK2195426T3 (da) 2007-10-05 2008-10-06 System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre.
KR1020107008929A KR101389254B1 (ko) 2007-10-05 2008-10-06 거대 게놈 ｄｎａ의 포획 및 변형 및 합성 엽록체를 갖는 생물의 구성을 위한 시스템
CA2698627A CA2698627A1 (fr) 2007-10-05 2008-10-06 Systeme permettant de capturer et de modifier de grands morceaux d'adn genomique, et de construire des organismes comprenant des chloroplastes synthetiques
MX2010003157A MX2010003157A (es) 2007-10-05 2008-10-06 Sistema para capturar y modificar piezas grandes de adn genomico y construir organismos con cloroplastos sinteticos.
EP08836521.8A EP2195426B1 (fr) 2007-10-05 2008-10-06 Système permettant de capturer et de modifier de grands morceaux d'adn génomique, et de construire des organismes comprenant des chloroplastes synthétiques
IL204250A IL204250A (en) 2007-10-05 2010-03-02 System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97802407P 2007-10-05 2007-10-05
US60/978,024 2007-10-05

Publications (2)

Publication Number Publication Date
WO2009045550A2 WO2009045550A2 (fr) 2009-04-09
WO2009045550A3 true WO2009045550A3 (fr) 2009-07-30

Family

ID=40042337

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/011540 Ceased WO2009045550A2 (fr) 2007-10-05 2008-10-06 Système permettant de capturer et de modifier de grands morceaux d'adn génomique, et de construire des organismes comprenant des chloroplastes synthétiques

Country Status (12)

Country Link
EP (1) EP2195426B1 (fr)
JP (1) JP5666908B2 (fr)
KR (1) KR101389254B1 (fr)
CN (1) CN101939423A (fr)
AU (1) AU2008307471B2 (fr)
CA (1) CA2698627A1 (fr)
DK (1) DK2195426T3 (fr)
GB (2) GB2460351B (fr)
IL (1) IL204250A (fr)
MX (1) MX2010003157A (fr)
NZ (1) NZ583682A (fr)
WO (1) WO2009045550A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101932725A (zh) 2007-10-08 2010-12-29 合成基因组股份有限公司 大核酸的装配
AU2010295739B2 (en) * 2009-09-15 2016-02-25 Renew Biopharma, Inc. A system for transformation of the chloroplast genome of Scenedesmus sp. and Dunaliella sp.
CN104277974B (zh) * 2013-07-03 2017-09-15 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701B (zh) * 2013-08-29 2019-01-11 天津大学 多片段dna的酵母快速组装方法
JP6226464B2 (ja) * 2013-09-30 2017-11-08 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
WO2016033088A1 (fr) * 2014-08-25 2016-03-03 GeneWeave Biosciences, Inc. Particules de transduction non réplicatives et systèmes rapporteurs les utilisant
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN110669789B (zh) * 2019-12-04 2020-04-14 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN111440733A (zh) * 2020-02-07 2020-07-24 天津大学 产松油醇的重组酿酒酵母及构建方法及应用
CN115948450B (zh) * 2022-08-01 2024-02-13 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN116218750B (zh) * 2022-10-14 2024-11-26 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用
CN118256412B (zh) * 2024-04-01 2024-09-27 湖北大学 运动发酵单胞菌的工程菌株、制备方法及应用

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US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof

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JP4858974B2 (ja) * 2004-03-03 2012-01-18 国立大学法人 奈良先端科学技術大学院大学 葉緑体工学による植物の生産性を向上させる方法
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US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector

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Also Published As

Publication number Publication date
GB2453648A (en) 2009-04-15
AU2008307471A1 (en) 2009-04-09
KR20100085930A (ko) 2010-07-29
GB2460351A (en) 2009-12-02
AU2008307471B2 (en) 2013-05-02
GB2453648C (en) 2012-12-05
JP5666908B2 (ja) 2015-02-12
GB0913302D0 (en) 2009-09-02
KR101389254B1 (ko) 2014-04-24
MX2010003157A (es) 2010-05-21
WO2009045550A2 (fr) 2009-04-09
EP2195426B1 (fr) 2013-06-12
GB2453648C2 (en) 2012-12-19
EP2195426A4 (fr) 2010-12-01
EP2195426A2 (fr) 2010-06-16
GB2453648B (en) 2011-01-05
JP2010539979A (ja) 2010-12-24
CN101939423A (zh) 2011-01-05
GB2453648A8 (en) 2010-11-17
IL204250A (en) 2012-12-31
GB2460351B (en) 2011-03-30
NZ583682A (en) 2012-07-27
CA2698627A1 (fr) 2009-04-09
DK2195426T3 (da) 2013-09-08
GB0818260D0 (en) 2008-11-12

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