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WO2009041633A1 - Procédé de marquage de protéine membranaire à l'aide d'une superhélice - Google Patents

Procédé de marquage de protéine membranaire à l'aide d'une superhélice Download PDF

Info

Publication number
WO2009041633A1
WO2009041633A1 PCT/JP2008/067518 JP2008067518W WO2009041633A1 WO 2009041633 A1 WO2009041633 A1 WO 2009041633A1 JP 2008067518 W JP2008067518 W JP 2008067518W WO 2009041633 A1 WO2009041633 A1 WO 2009041633A1
Authority
WO
WIPO (PCT)
Prior art keywords
membrane protein
coiled coil
peptide
conveniently
give
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/067518
Other languages
English (en)
Japanese (ja)
Inventor
Katsumi Matsuzaki
Yoshiaki Yano
Yukihiko Sugimoto
Gozoh Tsujimoto
Nobutaka Fujii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyoto University NUC
Original Assignee
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyoto University NUC filed Critical Kyoto University NUC
Priority to JP2009534428A priority Critical patent/JP5182671B2/ja
Publication of WO2009041633A1 publication Critical patent/WO2009041633A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé par lequel une protéine membranaire est exprimée sur une membrane cellulaire sans affecter l'emplacement ou la fonction de celle-ci, et est ainsi détectée de manière commode et rapide. Entre une paire de peptides d'hélice α formant un dimère à superhélice, le peptide à hélice négatif est fusionné avec une protéine membranaire cible pour donner un marqueur. D'autre part, l'autre peptide (à savoir, le peptide à hélice positive) constituant la paire de peptides d'hélice α formant le dimère à superhélice est lié à un colorant fluorescent pour donner une sonde. Ainsi, la protéine membranaire cible est liée de façon spécifique au colorant fluorescent, de telle sorte que la protéine membranaire est détectée de manière commode et rapide.
PCT/JP2008/067518 2007-09-28 2008-09-26 Procédé de marquage de protéine membranaire à l'aide d'une superhélice Ceased WO2009041633A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009534428A JP5182671B2 (ja) 2007-09-28 2008-09-26 コイルドコイルを利用した膜タンパク質標識方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007255648 2007-09-28
JP2007-255648 2007-09-28

Publications (1)

Publication Number Publication Date
WO2009041633A1 true WO2009041633A1 (fr) 2009-04-02

Family

ID=40511513

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/067518 Ceased WO2009041633A1 (fr) 2007-09-28 2008-09-26 Procédé de marquage de protéine membranaire à l'aide d'une superhélice

Country Status (2)

Country Link
JP (1) JP5182671B2 (fr)
WO (1) WO2009041633A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130295121A1 (en) * 2005-04-15 2013-11-07 Macrogenics, Inc. Covalent Diabodies and Uses Thereof
JP2015534076A (ja) * 2012-10-23 2015-11-26 イプセン バイオイノベーション リミテッド エンドソーム輸送評価試験法
US10336791B2 (en) 2005-12-22 2019-07-02 Vib Vzw Means and methods for mediating protein interference
US11872262B2 (en) 2017-05-09 2024-01-16 Vib Vzw Means and methods for treating bacterial infections

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109900814B (zh) * 2017-12-08 2021-06-08 中国科学院大连化学物理研究所 基于糖苷键质谱可碎裂型化学交联剂的分析方法及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006343207A (ja) * 2005-06-08 2006-12-21 Univ Of Tokyo 顕微鏡解析用プローブ及び顕微鏡解析方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006343207A (ja) * 2005-06-08 2006-12-21 Univ Of Tokyo 顕微鏡解析用プローブ及び顕微鏡解析方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LIM ET AL.: ""A General Methods for Selection and Screening of Coiled Coils on the Basis of Relative Helix Orientation"", J.AM.CHEM.SOC., vol. 124, 2002, pages 8237 - 8244 *
LITOWSKI ET AL.: ""Designing Heterodimeric Two- stranded a-Helical Coiled-Coil"", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 40, 2002, pages 37272 - 37279 *
YANO ET AL.: ""Coiled-Coil Tag-Probe Systems for Quick Labeling of Membrane Receptors in Living Cells"", ACS CHEMICAL BIOLOGY, vol. 3, no. 6, 2008, pages 341 - 345 *
YANO ET AL.: ""Specific Fluorescence Labeling of Membrane Receptors in Living Cells using Coild -Coil Tag"", PEPTIDE SCIENCE, 2007, pages 395 - 398 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130295121A1 (en) * 2005-04-15 2013-11-07 Macrogenics, Inc. Covalent Diabodies and Uses Thereof
US9889197B2 (en) * 2005-04-15 2018-02-13 Macrogenics, Inc. Covalently-associated diabody complexes that possess charged coil domains and that are capable of enhanced binding to serum albumin
US10336791B2 (en) 2005-12-22 2019-07-02 Vib Vzw Means and methods for mediating protein interference
JP2015534076A (ja) * 2012-10-23 2015-11-26 イプセン バイオイノベーション リミテッド エンドソーム輸送評価試験法
US11872262B2 (en) 2017-05-09 2024-01-16 Vib Vzw Means and methods for treating bacterial infections

Also Published As

Publication number Publication date
JP5182671B2 (ja) 2013-04-17
JPWO2009041633A1 (ja) 2011-01-27

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