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WO2008136057A1 - 電気泳動チップ及び電気泳動方法 - Google Patents

電気泳動チップ及び電気泳動方法 Download PDF

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Publication number
WO2008136057A1
WO2008136057A1 PCT/JP2007/000454 JP2007000454W WO2008136057A1 WO 2008136057 A1 WO2008136057 A1 WO 2008136057A1 JP 2007000454 W JP2007000454 W JP 2007000454W WO 2008136057 A1 WO2008136057 A1 WO 2008136057A1
Authority
WO
WIPO (PCT)
Prior art keywords
separation medium
aperture
flow channel
sample
separation
Prior art date
Application number
PCT/JP2007/000454
Other languages
English (en)
French (fr)
Inventor
Toru Kaji
Original Assignee
Shimadzu Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corporation filed Critical Shimadzu Corporation
Priority to PCT/JP2007/000454 priority Critical patent/WO2008136057A1/ja
Priority to US12/597,087 priority patent/US8834697B2/en
Priority to JP2009512795A priority patent/JP4831232B2/ja
Publication of WO2008136057A1 publication Critical patent/WO2008136057A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Electrostatic Separation (AREA)

Abstract

　透明な基板（１１）の内部に１本の流路（１２）が形成され、その両端は外方に開放された試料導入口（１３）、分離媒体充填口（１４）に接続され、さらに途中には外方に開放された試料排出口（１５）が設けられる。試料導入口（１３）と試料排出口（１５）との間が分離流路部（１２ａ）、試料排出口（１５）と分離媒体充填口（１４）との間が分離媒体導入流路部（１２ｂ）となる。分離媒体充填口（１４）に分離媒体充填装置を接続し分離流路部（１２ｂ）中に分離媒体を満たした後、分離媒体充填装置を接続したまま試料導入口（１３）に試料を滴下して分離流路部（１２ａ）に試料を導入した後、さらにバッファリザーバ（１６）、（１７）にバッファを溜めて泳動電圧を印加して泳動分析を行う。分離媒体充填装置を接続したまま繰り返し分析が行えるため、泳動の度に該充填装置を電気泳動チップから離脱させる必要がない。
PCT/JP2007/000454 2007-04-25 2007-04-25 電気泳動チップ及び電気泳動方法 WO2008136057A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PCT/JP2007/000454 WO2008136057A1 (ja) 2007-04-25 2007-04-25 電気泳動チップ及び電気泳動方法
US12/597,087 US8834697B2 (en) 2007-04-25 2007-04-25 Electrophoresis apparatus and a method for electrophoresis
JP2009512795A JP4831232B2 (ja) 2007-04-25 2007-04-25 電気泳動装置及び電気泳動方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/000454 WO2008136057A1 (ja) 2007-04-25 2007-04-25 電気泳動チップ及び電気泳動方法

Publications (1)

Publication Number Publication Date
WO2008136057A1 true WO2008136057A1 (ja) 2008-11-13

Family

ID=39943182

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/000454 WO2008136057A1 (ja) 2007-04-25 2007-04-25 電気泳動チップ及び電気泳動方法

Country Status (3)

Country Link
US (1) US8834697B2 (ja)
JP (1) JP4831232B2 (ja)
WO (1) WO2008136057A1 (ja)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011185714A (ja) * 2010-03-08 2011-09-22 Shimadzu Corp マイクロチップ電気泳動方法及び装置
JP2012202888A (ja) * 2011-03-28 2012-10-22 Shimadzu Corp 電気泳動デバイス及び電気泳動分析装置
JP2012202805A (ja) * 2011-03-25 2012-10-22 Shimadzu Corp 電気泳動装置
JP2014097463A (ja) * 2012-11-15 2014-05-29 Tadashi Kishimoto 電気泳動装置、電気泳動法および電気泳動法を用いた濃縮・分離・分析方法
WO2020067024A1 (ja) * 2018-09-25 2020-04-02 京セラ株式会社 検体処理デバイスへの検体導入方法
JP2021502556A (ja) * 2017-11-13 2021-01-28 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ エピタコ電気泳動を使用する試料分析のための装置
WO2023189163A1 (ja) * 2022-03-31 2023-10-05 京セラ株式会社 流路デバイスへの液体導入方法
JP2024546450A (ja) * 2021-12-10 2024-12-24 ビージーアイ シェンチェン マイクロ流体デバイスおよびマイクロ流体検出装置

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10967372B2 (en) * 2014-04-16 2021-04-06 International Business Machines Corporation Electro-fluidic flow probe

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001296275A (ja) * 2000-04-13 2001-10-26 Hitachi Ltd 電気泳動装置
JP2005274512A (ja) * 2004-03-26 2005-10-06 Aida Eng Ltd 電気泳動用マイクロチップ

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US5635050A (en) 1995-08-23 1997-06-03 Beckman Instruments, Inc. Electrophoretic system including means for replacing separation medium
GB9802600D0 (en) * 1998-02-07 1998-04-01 Eastman Kodak Co Liquid separation
US20020104759A1 (en) * 2000-12-07 2002-08-08 The Board Of Governors Of The University Of Alberta And Micralyne Inc. Sample analysis on a microchip
JP4362987B2 (ja) * 2001-04-09 2009-11-11 株式会社島津製作所 マイクロチップ電気泳動におけるサンプル導入方法
JP4492212B2 (ja) * 2004-05-20 2010-06-30 株式会社島津製作所 等電点電気泳動チップ及び装置
US7497937B2 (en) * 2004-09-03 2009-03-03 Combisep, Inc. Microfabricated chip and method of use
JP4375257B2 (ja) 2005-03-09 2009-12-02 株式会社島津製作所 キャピラリープレートを用いた電気泳動方法
JP4645255B2 (ja) * 2005-03-24 2011-03-09 株式会社島津製作所 分析試料調製用溶媒

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001296275A (ja) * 2000-04-13 2001-10-26 Hitachi Ltd 電気泳動装置
JP2005274512A (ja) * 2004-03-26 2005-10-06 Aida Eng Ltd 電気泳動用マイクロチップ

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011185714A (ja) * 2010-03-08 2011-09-22 Shimadzu Corp マイクロチップ電気泳動方法及び装置
JP2012202805A (ja) * 2011-03-25 2012-10-22 Shimadzu Corp 電気泳動装置
JP2012202888A (ja) * 2011-03-28 2012-10-22 Shimadzu Corp 電気泳動デバイス及び電気泳動分析装置
JP2014097463A (ja) * 2012-11-15 2014-05-29 Tadashi Kishimoto 電気泳動装置、電気泳動法および電気泳動法を用いた濃縮・分離・分析方法
US11691141B2 (en) 2017-11-13 2023-07-04 Roche Sequencing Solutions, Inc. Devices for sample analysis using epitachophoresis
JP2021502556A (ja) * 2017-11-13 2021-01-28 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ エピタコ電気泳動を使用する試料分析のための装置
JP7038209B2 (ja) 2017-11-13 2022-03-17 エフ．ホフマン－ラ ロシュ アーゲー エピタコ電気泳動を使用する試料分析のための装置
US12311357B2 (en) 2017-11-13 2025-05-27 Roche Sequencing Solutions, Inc. Devices for sample analysis using epitachophoresis
JPWO2020067024A1 (ja) * 2018-09-25 2021-08-30 京セラ株式会社 検体処理デバイスへの検体導入方法
JP7080334B2 (ja) 2018-09-25 2022-06-03 京セラ株式会社 検体処理デバイスへの検体導入方法
WO2020067024A1 (ja) * 2018-09-25 2020-04-02 京セラ株式会社 検体処理デバイスへの検体導入方法
JP2024546450A (ja) * 2021-12-10 2024-12-24 ビージーアイ シェンチェン マイクロ流体デバイスおよびマイクロ流体検出装置
WO2023189163A1 (ja) * 2022-03-31 2023-10-05 京セラ株式会社 流路デバイスへの液体導入方法
JP7374392B1 (ja) * 2022-03-31 2023-11-06 京セラ株式会社 流路デバイスへの液体導入方法

Also Published As

Publication number Publication date
US8834697B2 (en) 2014-09-16
JP4831232B2 (ja) 2011-12-07
JPWO2008136057A1 (ja) 2010-07-29
US20100116661A1 (en) 2010-05-13

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