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WO2008133929A3 - Cell permeable miniature proteins - Google Patents

Cell permeable miniature proteins Download PDF

Info

Publication number
WO2008133929A3
WO2008133929A3 PCT/US2008/005264 US2008005264W WO2008133929A3 WO 2008133929 A3 WO2008133929 A3 WO 2008133929A3 US 2008005264 W US2008005264 W US 2008005264W WO 2008133929 A3 WO2008133929 A3 WO 2008133929A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
miniature
miniature proteins
app
permeable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/005264
Other languages
French (fr)
Other versions
WO2008133929A2 (en
Inventor
Alanna S Schepartz
Douglas S Daniels
Betsy Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Publication of WO2008133929A2 publication Critical patent/WO2008133929A2/en
Publication of WO2008133929A3 publication Critical patent/WO2008133929A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/465—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention generally relates to miniature proteins, including miniature proteins that are permeable to cells. Certain aspects of the invention are generally related to miniature proteins, such as avian pancreatic polypeptide (aPP), modified such that the miniature proteins are permeable to cells. For instance, a portion of the aPP, such as the alpha helix region and/or the type II polyproline helix region, may be modified to render the region substantially cationic. As an example, one or more residues may be substituted with cationic amino acid residues such as arginine. The miniature proteins may also have additional functions, such as the ability to bind to other proteins such as Bcl2 or hDM2. Another aspect of the invention is generally directed to sequences, such as PRR or PPR, that can be added to other proteins in order to increase their cell permeability. Still other aspects of the invention are generally directed to methods of making such proteins, methods of using such proteins, kits involving such proteins, and the like.
PCT/US2008/005264 2007-04-24 2008-04-24 Cell permeable miniature proteins Ceased WO2008133929A2 (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US92637907P 2007-04-24 2007-04-24
US60/926,379 2007-04-24
US990508P 2008-01-03 2008-01-03
US61/009,905 2008-01-03
US1131108P 2008-01-16 2008-01-16
US61/011,311 2008-01-16
US6825908P 2008-03-05 2008-03-05
US61/068,259 2008-03-05

Publications (2)

Publication Number Publication Date
WO2008133929A2 WO2008133929A2 (en) 2008-11-06
WO2008133929A3 true WO2008133929A3 (en) 2008-12-24

Family

ID=39764715

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/005264 Ceased WO2008133929A2 (en) 2007-04-24 2008-04-24 Cell permeable miniature proteins

Country Status (1)

Country Link
WO (1) WO2008133929A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110818804B (en) * 2019-10-21 2021-06-04 兰州大学 Brain targeting peptide and application thereof in preparation of memory enhancing drugs

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014169274A2 (en) * 2013-04-12 2014-10-16 Yale University Modified proteins and methods of use thereof
JP6495270B2 (en) 2013-10-30 2019-04-03 ザ ユニバーシティ オブ ウェスタン オーストラリア Pharmaceutical composition containing a neuroprotective peptide
EP3145523A4 (en) * 2014-05-21 2018-02-21 President and Fellows of Harvard College Ras inhibitory peptides and uses thereof

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CAESAR CHRISTINA E B ET AL: "Membrane interactions of cell-penetrating peptides probed by tryptophan fluorescence and dichroism techniques: Correlations of structure to cellular uptake", BIOCHEMISTRY, vol. 45, no. 24, June 2006 (2006-06-01), pages 7682 - 7692, XP002498266, ISSN: 0006-2960 *
CHIN J W ET AL: "Concerted evolution of structure and function in a MINIATURE PROTEIN", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC.; US, US, vol. 123, 28 March 2001 (2001-03-28), pages 2929 - 2930, XP002196758, ISSN: 0002-7863 *
DANIELS DOUGLAS S ET AL: "Intrinsically cell-permeable miniature proteins based on a minimal cationic PPII motif.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 28 NOV 2007, vol. 129, no. 47, 28 November 2007 (2007-11-28), pages 14578 - 14579, XP002498265, ISSN: 1520-5126 *
NIARCHOS ET AL: "Characterization of a novel cell penetrating peptide derived from Bag-1 protein", PEPTIDES, ELSEVIER, AMSTERDAM, vol. 27, no. 11, 1 November 2006 (2006-11-01), pages 2661 - 2669, XP005713903, ISSN: 0196-9781 *
SADLER KRISTEN ET AL: "Translocating proline-rich peptides from the antimicrobial peptide bactenecin 7.", BIOCHEMISTRY, vol. 41, no. 48, 3 December 2002 (2002-12-03), pages 14150 - 14157, XP002498267, ISSN: 0006-2960 *
SMITH BETSY A ET AL: "Minimally cationic cell-permeable miniature proteins via alpha-helical arginine display.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 12 MAR 2008, vol. 130, no. 10, 12 March 2008 (2008-03-12), pages 2948 - 2949, XP002498264, ISSN: 1520-5126 *
THOREN P E G ET AL: "Uptake of analogs of penetratin, Tat(48-60) and oligoarginine in live cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 307, no. 1, 18 July 2003 (2003-07-18), pages 100 - 107, XP004434078, ISSN: 0006-291X *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110818804B (en) * 2019-10-21 2021-06-04 兰州大学 Brain targeting peptide and application thereof in preparation of memory enhancing drugs

Also Published As

Publication number Publication date
WO2008133929A2 (en) 2008-11-06

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