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WO2008125116A3 - Upar inhibition - Google Patents

Upar inhibition Download PDF

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Publication number
WO2008125116A3
WO2008125116A3 PCT/DK2008/050075 DK2008050075W WO2008125116A3 WO 2008125116 A3 WO2008125116 A3 WO 2008125116A3 DK 2008050075 W DK2008050075 W DK 2008050075W WO 2008125116 A3 WO2008125116 A3 WO 2008125116A3
Authority
WO
WIPO (PCT)
Prior art keywords
aptamers
present
prevention
proteolytic activity
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2008/050075
Other languages
French (fr)
Other versions
WO2008125116A2 (en
Inventor
Joergen Kjems
Daniel Miotto Dupont
Peter A Andreasen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aarhus Universitet
Original Assignee
Aarhus Universitet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aarhus Universitet filed Critical Aarhus Universitet
Publication of WO2008125116A2 publication Critical patent/WO2008125116A2/en
Publication of WO2008125116A3 publication Critical patent/WO2008125116A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification

Landscapes

  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to aptamers capable of binding to a receptor-binding form of urokinase-type plasminogen activator protein, u-PA. Furthermore, the present invention relates to use of the aptamers for prevention of u-PA-mediated proteolytic activity at the cell surface for the preparation of a composition for preventing or counteracting proteolytic activity on the surface of a cell. The aptamers of the present invention may additionally be used as imaging agents or used for the treatment or prevention of cancer and/or metastatic spread. Within the scope of the present invention is also pharmaceutical compositions and diagnostic kits comprising aptamers.
PCT/DK2008/050075 2007-03-31 2008-03-28 Upar inhibition Ceased WO2008125116A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200700506 2007-03-31
DKPA200700506 2007-03-31
DKPA200701559 2007-11-01
DKPA200701559 2007-11-01

Publications (2)

Publication Number Publication Date
WO2008125116A2 WO2008125116A2 (en) 2008-10-23
WO2008125116A3 true WO2008125116A3 (en) 2009-03-12

Family

ID=39864405

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2008/050075 Ceased WO2008125116A2 (en) 2007-03-31 2008-03-28 Upar inhibition

Country Status (1)

Country Link
WO (1) WO2008125116A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011341018A1 (en) * 2010-12-10 2013-07-25 Merck Patent Gmbh Aptamer labeled with F-19 nucleus for targeted molecular imaging by MRI
ES2781523T3 (en) 2012-07-12 2020-09-02 Hangzhou Dac Biotech Co Ltd Conjugates of cell binding molecules with cytotoxic agents
JP6133431B2 (en) 2012-11-24 2017-05-24 ハンジョウ ディーエーシー バイオテック シーオー．，エルティディ．Ｈａｎｇｚｈｏｕ Ｄａｃ Ｂｉｏｔｅｃｈ Ｃｏ．，Ｌｔｄ． Use of hydrophilic conjugates and conjugation reactions between drug molecules and cell binding molecules
US10464955B2 (en) 2014-02-28 2019-11-05 Hangzhou Dac Biotech Co., Ltd. Charged linkers and their uses for conjugation
CN113350518A (en) 2015-07-12 2021-09-07 杭州多禧生物科技有限公司 Conjugated bridge linkers to cell binding molecules
US9839687B2 (en) 2015-07-15 2017-12-12 Suzhou M-Conj Biotech Co., Ltd. Acetylenedicarboxyl linkers and their uses in specific conjugation of a cell-binding molecule
CN106497919A (en) 2016-11-02 2017-03-15 四川大学 A kind of DNA tetrahedrons of aptamer AS1411 modifications and preparation method thereof
CN116143678A (en) 2016-11-14 2023-05-23 杭州多禧生物科技有限公司 Conjugate linker, cell-binding molecule-drug conjugate containing the same, and preparation and application thereof
WO2019165105A1 (en) * 2018-02-26 2019-08-29 The Board Of Trustees Of The Leland Stanford Junior University Ligands of the urokinase receptor and their use in treating, detecting, and imaging cancer
PH12021553156A1 (en) 2019-06-29 2022-07-25 Hangzhou Dac Biotech Co Ltd Conjugates of tubulysin derivatives and cell binding molecules and methods of making
CN111453729B (en) * 2019-08-26 2021-06-18 山东大学 A kind of method and application of ultrasonic-assisted preparation of super activated carbon
KR20240095442A (en) 2021-11-03 2024-06-25 항저우 디에이씨 바이오테크 씨오, 엘티디 Specific conjugation of antibodies
EP4194555A1 (en) * 2021-12-09 2023-06-14 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Dna aptamers inhibiting binding of urokinase type plasminogen activator (upa) with urokinase type plasminogen activator receptor (upar) and inhibiting upa activity

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
HAMULA C.L.A. ET AL.: "Selection and analytical applications of Aptamers", TRENDS IN ANALYTICAL CHEMISTRY, vol. 25, no. 7, 2006, pages 681 - 691, XP002509244 *
JAYASENA S.D.: "Aptamers: an emerging class of molecules that rival antibodies in diagnostics", CLINICAL CHEMISTRY, vol. 45, no. 9, 1999, pages 1628 - 1650, XP002509245 *
SCHMITT M. ET AL.: "The Urokinase Plasminogen Activator system as a novel target for tumour therapy", FIBRINOLYSIS & PROTEOLYSIS, vol. 14, no. 2/3, 2000, pages 114 - 132, XP002509198 *
SCHWEINITZ A. ET AL.: "Design of novel and selective inhibitors of urokinase-type Plasminogen Activator with improved pharmakokinetic properties for use as antimetastatic agents", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, no. 32, 6 August 2004 (2004-08-06), pages 33613 - 33622, XP002509197 *
SKRYPINA N.A. ET AL.: "In vitro selection of single-stranded DNA aptamers that bind human pro-urokinase", NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS, vol. 23, no. 6-7, October 2004 (2004-10-01), pages 891 - 893, XP008100024 *
ULRICH H.: "DNA and RNA Aptamers as modulators of protein function", MEDICINAL CHEMISTRY, BENTHAM SCIENCE PUBLISHERS LTD, BUSSUM, NL, vol. 1, no. 2, 1 March 2005 (2005-03-01), pages 199 - 208, XP001248936, ISSN: 1573-4064 *
ULRICH H.: "RNA Aptamers: from basic science towards therapy", HANDBOOK OF EXPERIMENTAL PHARMACOLOGY, vol. 173, 2006, pages 305 - 326, XP001249105 *

Also Published As

Publication number Publication date
WO2008125116A2 (en) 2008-10-23

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