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WO2008115517A3 - Methods for identifying stem cells by detecting autofluorescence of cells and syncytia - Google Patents

Methods for identifying stem cells by detecting autofluorescence of cells and syncytia Download PDF

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Publication number
WO2008115517A3
WO2008115517A3 PCT/US2008/003604 US2008003604W WO2008115517A3 WO 2008115517 A3 WO2008115517 A3 WO 2008115517A3 US 2008003604 W US2008003604 W US 2008003604W WO 2008115517 A3 WO2008115517 A3 WO 2008115517A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
syncytia
methods
stem cells
identifying stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/003604
Other languages
French (fr)
Other versions
WO2008115517A2 (en
Inventor
Elena V Gostjeva
William G Thilly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of WO2008115517A2 publication Critical patent/WO2008115517A2/en
Publication of WO2008115517A3 publication Critical patent/WO2008115517A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57496—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving intracellular compounds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Methods are disclosed for the characterization of the stage of development or pathology of a tissue sample, and for identifying pluripotent stem cells, comprising detecting autofluoresence of cells and syncytia.
PCT/US2008/003604 2007-03-19 2008-03-19 Methods for identifying stem cells by detecting autofluorescence of cells and syncytia Ceased WO2008115517A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91880807P 2007-03-19 2007-03-19
US60/918,808 2007-03-19

Publications (2)

Publication Number Publication Date
WO2008115517A2 WO2008115517A2 (en) 2008-09-25
WO2008115517A3 true WO2008115517A3 (en) 2009-03-12

Family

ID=39529613

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/003604 Ceased WO2008115517A2 (en) 2007-03-19 2008-03-19 Methods for identifying stem cells by detecting autofluorescence of cells and syncytia

Country Status (1)

Country Link
WO (1) WO2008115517A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005265122A1 (en) 2004-06-17 2006-01-26 Massachusetts Institute Of Technology Methods for identifying stem cells based on nuclear morphotypes
US8951938B2 (en) 2007-12-20 2015-02-10 New York University Combinatorial rosamine library to detect cell-state switching
WO2010036322A1 (en) * 2008-09-24 2010-04-01 Massachusetts Institute Of Technology Methods for identifying stem cells by detecting fluorescence of cells and syncytia
US8735150B2 (en) 2010-08-24 2014-05-27 New York University Methods for detecting embryonic stem cells, induced pluripotent stem cells, or cells undergoing reprogramming to produce induced pluripotent stem cells
WO2012061073A1 (en) 2010-10-25 2012-05-10 Massachusetts Institute Of Technology Wound healing metakaryotic stem cells and methods of use thereof
WO2014072960A2 (en) * 2012-11-12 2014-05-15 National Centre For Biological Sciences A method to identify and isolate pluripotent stem cells using endogenous blue fluorescence
CN109254142B (en) * 2018-11-08 2025-04-15 贵阳德漫多医疗科技有限公司 A system and detection method for identifying tumor stem cells and detecting their drug sensitivity

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006009860A2 (en) * 2004-06-17 2006-01-26 Massachusetts Institute Of Technology Methods for identifying stem cells based on nuclear morphotypes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006009860A2 (en) * 2004-06-17 2006-01-26 Massachusetts Institute Of Technology Methods for identifying stem cells based on nuclear morphotypes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GOSTJEVA E V ET AL: "Nuclear morphotypes in human embryogenesis and carcinogenesis: Bell-shaped nuclei show stem-like properties in vivo.", ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, vol. 47, no. 6, July 2006 (2006-07-01), & 37TH ANNUAL MEETING OF THE ENVIRONMENTAL-MUTAGEN-SOCIETY; VANCOUVER, CANADA; SEPTEMBER 16 -20, 2006, pages 405, XP009102297, ISSN: 0893-6692 *
GOSTJEVA ET AL: "Bell-shaped nuclei dividing by symmetrical and asymmetrical nuclear fission have qualities of stem cells in human colonic embryogenesis and carcinogenesis", CANCER GENETICS AND CYTOGENETICS, ELSEVIER SCIENCE PUBLISHING, NEW YORK, NY, US, vol. 164, no. 1, 1 January 2006 (2006-01-01), pages 16 - 24, XP005214800, ISSN: 0165-4608 *

Also Published As

Publication number Publication date
WO2008115517A2 (en) 2008-09-25

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